Variability Studies in Melon (Cucumis melo.L) F2 Population of Kashi Madhu x COHB38 for Powdery Mildew Resistance

2019 ◽  
Vol 7 (4) ◽  
pp. 407-413
Author(s):  
K.R. Kavya ◽  
Manchali Shivapriya
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Continuous Distribution ◽  
F2 Population ◽  
Progress Curve ◽  
Powdery Mildew Disease ◽  
Percent Disease Index ◽  
Natural Field Condition ◽  
Moderately Resistant ◽  
Segregating Population

The present investigation was carried at College of Horticulture, Bengaluru. Evaluation of melon F2 population (Kashi Madhu X COHB38) was conducted during rabi 2016 for resistance to powdery mildew disease under natural field condition. Percent Disease Index (PDI) and AUDPC (Area Under Disease Progress Curve) value was calculated to assess the reaction of F2 (Kashi Madhu X COHB38) segregating population of melon along with the parents (COHB38 and Kashi Madhu) and F1. Based on PDI for powdery mildew disease, 152 F2 plants were classified into different categories. Twenty five F2s were resistant (0-25% PDI), 27 were moderately resistant (25.1-40% PDI), 88 were susceptible (40.1-60% PDI) and 12 F2s were highly susceptible (>60% PDI). Among 152 F2 plants,    F2 -34 was found to be highly resistant with zero PDI and zero AUDPC value (no disease) followed by F2- 46 and 92 with PDI of 13.89 % and 16.11 % and 46.94 and 55.00 AUDPC value, respectively. The PDI of the disease showed a continuous distribution from highly resistant to highly susceptible phenotypes, without showing any typical segregation pattern. Int. J. Appl. Sci. Biotechnol. Vol 7(4): 407-413

scholarly journals Evaluation of Powdery Mildew Resistance in Hydrangea macrophylla

2011 ◽  
Vol 29 (2) ◽  
pp. 60-64 ◽  
Author(s):  
Mark T. Windham ◽  
Sandra M. Reed ◽  
Margaret T. Mmbaga ◽  
Alan S. Windham ◽  
Yonghao Li ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Late Summer ◽  
Sources Of Resistance ◽  
Mildew Resistance ◽  
Potted Plants ◽  
Hydrangea Macrophylla ◽  
Erysiphe Polygoni ◽  
High Level ◽  
Moderately Resistant

Abstract Powdery mildew, Erysiphe polygoni DC, can be a significant problem on Hydrangea macrophylla (Thunb.) Ser. in the landscape in late summer to fall and during greenhouse propagation or production of potted plants. Because very little information related to sources of resistance is available, 90 H. macrophylla cultivars were evaluated for resistance to powdery mildew over a 3-year period. This included 69 H. macrophylla ssp. macrophylla and 18 H. macrophylla ssp. serrata (Thunb.) Makino cultivars, along with three cultivars that are hybrids between the two subspecies. Significant differences among cultivars to powdery mildew were found in all three study years. Three cultivars, ‘Amagi Amacha’, ‘Shirofuji’ and ‘Veitchii’, were among the most resistant each year. ‘Diadem’, ‘Komachi’, and ‘Omacha’ were highly resistant in 2006 and 2008, but only moderately resistant in 2007. ‘Komachi’ and ‘Shirofuji’ were considered unsuitable for breeding purposes as they do not appear to produce fertile flowers. ‘Veitchii’ was the only member of H. macrophylla ssp. macrophylla with a high level of powdery mildew resistance; while all other resistant cultivars were members of H. macrophylla ssp. serrata, not all members of this subspecies are resistant. Results of this study will be useful in breeding powdery mildew resistant H. macrophylla.

Genetic Mapping of Adult-Plant Resistance Genes to Powdery Mildew in Triticale

2021 ◽  
Author(s):  
Mateusz Maksymilian Dyda ◽  
Mirosław Tyrka ◽  
Gabriela Gołębiowska ◽  
Marcin Rapacz ◽  
Maria Wędzony
Keyword(s):  
Powdery Mildew ◽  
Candidate Genes ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Doubled Haploids ◽  
Rapid Development ◽  
Genetic Maps ◽  
Adult Plant ◽  
Mildew Resistance ◽  
Progress Curve

Abstract Triticale is a cereal of high economic importance, however along with the increase in the area of this cereal, it is more often infected by the fungal pathogen Blumeria graminis, which causes powdery mildew. The rapid development of molecular biology techniques, in particular methods based on molecular markers may be an important tool used in modern plant breeding. Development of genetic maps, location of the QTLs defining the region of the genome associated with resistance and selection of markers linked to particular trait can be used to select resistant genotypes as well as to pyramidize several resistance genes in one variety. In this paper we present a new, high-density genetic map of triticale doubled haploids (DH) population ‘Grenado’ x ‘Zorro’ composed of DArT, silicoDArT and SNP markers. Composite interval mapping method was used to detect eight QTL regions associated with the area under disease progress curve (AUDPC) and 15 regions with the average value of powdery mildew infection (avPM) based on observation conducted in 3-year period in three different locations across the Poland. Two regions on rye chromosome 4R, and single loci on 5R and 6R were reported for the first time as regions associated with powdery mildew resistance. Among all QTLs, 14 candidate genes were identified coded cyclin-dependent kinase, serine/threonine-protein kinase-like protein as well as AMEIOTIC 1 homolog DYAD-like protein, DETOXIFICATION 16-like protein and putative disease resistance protein RGA3. Three of identified candidate genes were found among newly described QTL regions associated with powdery mildew resistance in triticale.

scholarly journals Susceptibility of Eastern Ninebark (Physocarpus opulifolius (L.) Maxim.) Cultivars to Powdery Mildew

2011 ◽  
Vol 29 (3) ◽  
pp. 105-107 ◽  
Author(s):  
J.D. Lubell ◽  
M.H. Brand ◽  
J.M. Lehrer
Keyword(s):  
Powdery Mildew ◽  
Disease Severity ◽  
Powdery Mildew Resistance ◽  
The Other ◽  
Mildew Resistance ◽  
Leaf Drop ◽  
Powdery Mildew Disease ◽  
Physocarpus Opulifolius ◽  
Morning Star

Abstract Powdery mildew disease severity was assessed on ten eastern ninebark (Physocarpus opulifolius (L.) Maxim.) cultivars. The green foliage cultivar ‘Nanus’ was resistant to powdery mildew. Among the deep purple foliage cultivars, ‘ Seward’ Summer Wine® exhibited better resistance than ‘Monlo’ Diablo® and was nearly as resistant as ‘Nanus’. ‘Seward’ Summer Wine® is a hybrid between ‘Nanus’ and ‘Monlo’ Diablo® and probably derives its mildew resistance from ‘Nanus’. ‘Monlo’ Diablo® had reasonably good mildew resistance. Yellow foliage cultivars ‘Dart's Gold’, ‘Morning Star’ and ‘Nugget’, which were highly susceptible to powdery mildew, were unattractive due to substantial leaf drop, leaf disfigurement and shoot brooming. ‘Luteus’ exhibited better powdery mildew resistance than the other yellow foliage cultivars. ‘Mindia’ Coppertina® and ‘Center Glow’, two recent purple foliage introductions with orange-copper new foliage, exhibited levels of mildew intermediate between purple and yellow foliage cultivars. These ‘Monlo’ Diablo® × ‘Dart's Gold’ hybrids probably owe their reduced mildew resistance to their ‘Dart's Gold’ lineage.

scholarly journals Screening of sesame genotypes against powdery mildew disease caused by Erysiphe cichorecearum

2020 ◽  
Vol 13 (2) ◽  
pp. 175-179
Author(s):  
S.D. Jagtap ◽  
B.C. Game ◽  
P.E. More
Keyword(s):  
Powdery Mildew ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Combining Ability ◽  
Yield Losses ◽  
Night Temperature ◽  
Genetic Character ◽  
Powdery Mildew Disease ◽  
Sesame Genotypes ◽  
Moderately Resistant

Powdery mildew disease of sesame occurs on epidemic scale in areas of high rainfall and humidity coupled with low night temperature causing considerable yield losses. Use of host plant resistance is the practical approach to manage this disease, but proper resistance sources with combining ability for the trait are unknown. Hence, an experiment was conducted to determine resistance in sesame genotypes against powdery mildew disease. Among the twenty four genotypes screened, none was found resistant while, nine genotypes exhibited moderately resistant to tolerant reaction and 15 genotypes exhibited susceptible reaction. Apparent rate of infection value varied and at times they did not remain consistent for given genotype and also did not show a particular trend which is attributed to genetic character of the genotype. The AUDPC values differed considerably for different genotypes. The values of AUDPC and apparent rate of infection of susceptible varieties were high as compared to moderately susceptible varieties. Genotype ‘JLS-302-11’ and ‘JLT-7’ having minimum AUDPC and apparent rate of infection value showed lowest intensity of powdery mildew while, genotype ‘JLT-408’ having maximum AUDPC and apparent rate of infection value showed highest intensity of powdery mildew.

scholarly journals HvGCN2 silencing in barley displays enhanced Blumeria graminis f. sp. hordei susceptibility

2018 ◽  
Vol 70 (3) ◽  
pp. 513-520
Author(s):  
Ibrahim Ozturk ◽  
Figen Ersoy ◽  
Mahinur Akkaya
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Blumeria Graminis ◽  
Virus Induced Gene Silencing ◽  
Incompatible Interaction ◽  
Resistance Response ◽  
Mildew Resistance ◽  
Barley Powdery Mildew ◽  
Powdery Mildew Disease ◽  
Crop Disease

Powdery mildew disease, caused by Blumeria graminis f. sp. hordei (Bgh), which belongs to the order Erysiphales, is a major crop disease. The general control nondepressible-2 (GCN2) gene of barley was previously found to be overexpressed during the powdery mildew resistance response. Recently, Arabidopsis thaliana GCN2 (AtGCN2) was shown to be involved in disease resistance against biotrophic and necrotrophic pathogens. In order to understand the function of Hordeum vulgare GCN2 (HvGCN2) in the barley powdery mildew resistance response, this gene was silenced by barley stripe mosaic virus (BSMV), mediated by virus-induced gene silencing (VIGS). This is the first study showing the potential importance of HvGCN2 in powdery mildew disease of barley. Based on our observations, when HvGCN2 was silenced on average by 53.5%, Bgh development was increased by 18.7 to 32.1%, which was determined by primary, secondary and longest hyphae measurements. The number of germinated spores also increased 2.8-fold in HvGCN2 silenced plants compared to control plants (BSMV:00). On the other hand, under the resistant condition, no difference was observed in HvGCN2- silenced plants compared to non-silenced lines although the gene was found to be overexpressed in incompatible interaction.

scholarly journals Powdery Mildew Resistance of the Lithuanian Winter Wheat Breeding Material

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Žilvinas Liatukas ◽  
Vytautas Ruzgas
Keyword(s):  
Powdery Mildew ◽  
Winter Wheat ◽  
Powdery Mildew Resistance ◽  
Quantitative Resistance ◽  
Wheat Breeding ◽  
Resistance Level ◽  
Mildew Resistance ◽  
Breeding Material ◽  
Progress Curve ◽  
Wheat Lines

Powdery Mildew Resistance of the Lithuanian Winter Wheat Breeding Material At the Lithuanian Institute of Agriculture, during 2004-2006, resistance to powdery mildew of approximately 1,500 winter wheat lines was assessed in check and competitive trial nurseries. Our experimental evidence showed that there were no genotypes with effective resistance singlegenes among the lines tested. Effective powdery mildew resistance from start to end of vegetation season depended on the quantitative resistance level. The most resistant lines were evaluated with a score of 2 and area under the disease progress curve (AUDPC) values ranging between 1.0-5.4. The most susceptible genotypes from the collection nurseries had score 8-9 and AUDPC values ranging between 1350-2220. The correlations between maximal disease severity and AUDPC values were strong (r = 0.79-0.92). Genotypes with AUDPC values up to 10 represented 93 lines or 7% in the check trial nursery and 22 lines or 9% in the competitive trial nursery. Lines evaluated with a score 4-5 and AUDPC value 100-300 dominated in 2004. In the next year the dominant genotypes had resistance scores 3-4 and AUDPC value 50-200. The highest powdery mildew resistance (score 2 and AUDPC value 1.0) was identified for the lines Maverich/Victo, Flair/Lut.9392, Strumok/Lut.9321, Zentos/Lut.97-6, Strumok/Lut.9313, Dirigent/Cortez in 2006.

Genetic Diversity Analysis and Validation of Microsatellite Markers Linked with Tolerance to Powdery Mildew Disease in Mungbean [Vigna radiata (L.) Wilczek]

2021 ◽  
Author(s):  
B.S. Pavithra ◽  
Laxmipreeya Behera ◽  
K.C. Samal
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Vigna Radiata ◽  
Powdery Mildew Resistance ◽  
Grain Legume ◽  
Phenotypic Traits ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
A Genome ◽  
Powdery Mildew Disease

Background: Mungbean [Vigna radiata (L.) Wilczek] is a self-pollinated diploid grain legume (2n=2x=22) crop and has a genome size of 560 Mb. The present study was concentrate to portray the nature and extent of genotypic variation exists among mungbean collections for a range of traits of potential agronomic and adaptive interests. Many diseases affect mungbean, causes the major constraint in the increasing production among which Powdery mildew disease caused by Erisyphepolygoni is economically significant because it reduces photosynthetic activity and physiological changes which results in 20-40 per cent reduction in yield. Some gene-specific marker were analysed and found associated with powdery mildew resistance in mungbean genotypes.Methods: The present investigation was carried out to evaluate thirty-one mungbean genotypes (including four checks) collected from the different parts of India. The genotypes were sown in an incomplete augmented bock design along with four checks varieties. viz. ‘Kamdev’, ‘OBBGG-52’, ‘IPM-02-14’ and ‘IPM-02-3’. All the mungbean genotypes were evaluated for different phenotypic traits and their tolerance to powdery mildew disease at two cropping seasons as well as at two different locations in Odisha. In this investigation, seven molecular markers viz., VrCSSTS1, VrCSSTS2, VrCSSSR3, CEDG191, MB-SSR238, CEDG166, CEDG282 were analysed.Result: SSR marker such as VrCSSSR and VrCSSTS linked with powdery mildew resistance gene were tested in different genotypes with known powdery mildew reaction and the results showed a consistent association of the marker in all the powdery mildew resistant genotypes and absent in all the powdery mildew susceptible genotypes. The results confirmed the validation of these markers with the powdery mildew resistance gene in different genetic backgrounds. Similarly, CEDG191, CEDG166, CEDG282 markers, reported to be linked to powdery mildew resistance, amplified the respective marker fragment of 100 to 300 bp in mungbean genotypes and were polymorphic. The above PCR-based and locus-specific markers could be employed for marker-assisted breeding (MAB) program as well as genotype conservation. These linked markers will boost the efficiency and precision of powdery mildew resistance breeding in mungbean.

Evaluation of resistant genotypes and their characterization using molecular markers linked for powdery mildew resistance in cucumber (Cucumis sativus L.)

2022 ◽  
pp. 1-6
Author(s):  
Susheel Sharma ◽  
Aejaz Ahmad Dar ◽  
Sachin Gupta ◽  
Ravinder Singh
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Breeding Strategies ◽  
Linkage Studies ◽  
Cucumis Sativus L ◽  
Moderately Resistant ◽  
Resistant Genotypes

Abstract Powdery mildew (PM) is one of the most severe fungal diseases of cucumber that limits its production worldwide. In this study, 140 genotypes of cucumber were screened for disease resistance under field and artificial conditions, and then validated with eight known SSR markers linked to PM resistance. Among these genotypes, genotype GS140 was found resistant (R), whereas GS148, GS16 and GS70 were moderately resistant, and GS169 was found to be tolerant. Of all the eight markers, only C31, C80, C162, SSR16472 and SSR16881 amplified the reported linked allele. The 127 bp allele of SSR16881 was found to be associated with the lowest disease severity of 37.65%. The associated markers could further be verified for their usability using linkage studies and the contrast genotypes in the present study could serve as a tool for selection in future marker-assisted selection breeding strategies for PM resistance.

scholarly journals Validation of molecular marker associated with powdery mildew resistance in mungbean

2018 ◽  
Vol 78 (3) ◽  
Author(s):  
Sumangala Bhat ◽  
S. K. Pooja ◽  
A. P. Sarkale
Keyword(s):  
Powdery Mildew ◽  
Ssr Markers ◽  
Ssr Marker ◽  
Powdery Mildew Resistance ◽  
Phenotypic Variance ◽  
Single Marker Analysis ◽  
Mildew Resistance ◽  
F2 Population ◽  
Single Marker ◽  
Erysiphe Polygoni

Powdery mildew caused by Erysiphe polygoni, is one of the major diseases of mungbean (Vigna radiata L. Wilczek), causes yield loss up to 20-40%. The present investigation was undertaken to study the SSR markers associated with powdery mildew resistance in mungbean. The resistant line, TARM1 was crossed with highly susceptible, but popular variety DGGV2 and the F1s were selfed to obtain F2. F2 population was evaluated for response to PM under field conditions. Of the 64 SSR markers studied, only four were found to be polymorphic between two parents (TARM1 and DGGV2). Single marker analysis indicated that one SSR marker (MB-SSR238) showed association with powdery mildew resistance in mungbean, explaining the phenotypic variance of 11.64%.

scholarly journals Field screening of rice (Oryza sativa L.) genotypes for resistance to bacterial leaf blight (Xanthomonas oryzae pv. oryzae) disease

2021 ◽  
Vol 4 (1) ◽  
pp. 42-49
Author(s):  
Basistha Acharya ◽  
Sujata KC
Keyword(s):  
Oryza Sativa L ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Progress Curve ◽  
Natural Field Condition ◽  
Rice Genotypes ◽  
Different Genetic Background ◽  
Moderately Resistant ◽  
Resistant Genotypes

Rice is the most important crop in Nepal followed by maize and wheat in terms of area (1.4 million ha), production (5.15 million) and productivity (3.5 mt/ha). Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae is considered to be the most widespread and destructive disease of rice in both irrigated and rainfed environments in Nepal. Use of host plant resistance is the most feasible and economical way to combat this disease. However, breakdown of resistance of a variety often occurs after few years of release due to genetic adaptation of the pathogen. Regular screening of the genotypes is utmost for developing the resistant genotypes. Considering the point, this investigation effort has been made to screen the rice genotypes against BLB under natural field condition at Khajura, Banke during 2018 and 2019. In the study, plants were assessed by measuring disease severity (percentage of leaf diseased) and area under the disease progress curve (AUDPC). Differences in resistance among the rice genotypes were observed in both of the tested year. Among the screened 150 rice genotypes during 2018, 6 genotypes scored resistant (R), 81 moderately resistant (MR), 59 moderately susceptible (MS) and 4 susceptible (S) to BLB. Whereas, in 2019, among the tested 315 rice genotypes, none of the genotypes were resistant, 183 MR, 131 MS and 1 S to BLB disease. This showed that the tested genotypes were composed of different genetic background.  

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