scholarly journals Biodegradation of spent engine oil by bacteria isolated from the rhizosphere of legumes grown in contaminated soil

2014 ◽  
Vol 3 (2) ◽  
pp. 63-75 ◽  
Author(s):  
HY Ismail ◽  
UJJ Ijah ◽  
ML Riskuwa ◽  
II Allamin
Keyword(s):  
Molecular Weight ◽  
Contaminated Soil ◽  
Rhizosphere Soil ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Lablab Purpureus ◽  
Degrading Bacteria ◽  
Spent Engine Oil

Biodegradation of spent engine oil (SEO) by bacteria isolated from the rhizosphere of Cajan cajan and Lablab purpureus was investigated. It was with a view to determining most efficient bacterial species that could degrade SEO in phytoremediation studies. Hydrocarbon degrading bacteria were isolated and identified by enrichment culture technique using oil agar supplemented with 0.1% v/v SEO. Total heterotrophic and oil utilizing bacterial count showed the occurrence of large number of bacteria predominantly in the rhizosphere soil, ranging between 54×108 - 144×108 CFU/g and 4×108- 96×108 CFU/g respectively. Percentage of oil utilizing bacteria ranged between 0% (uncontaminated non rhizosphere soil) to 76% (contaminated rhizosphere). Turbidimetrically, five bacterial species namely Pseudomonas putrefacience CR33, Klebsiella pneumonia CR23, Pseudomonas alcaligenes LR14, Klebsiella aerogenes CR21, and Bacillus coagulans CR31 were shown to grow maximally and degraded the oil at the rate of 68%, 62%, 59%, 58%and 45% respectively. Chromatographic analysis using GC-MS showed the presence of lower molecular weight hydrocarbons in the residual oil (indicating degradation) after 21 days, whereas the undegraded oil (control) had higher molecular weight hydrocarbons after the same period. The species isolated were shown to have high ability of SEO biodegradation and therefore could be important tools in ameliorating SEO contaminated soil. DOI: http://dx.doi.org/10.3126/ije.v3i2.10515 International Journal of the Environment Vol.3(2) 2014: 63-75

scholarly journals Microbial Treatment of Soil Contaminated with Spent engine Oil / Biotreatment of Soil Contaminated with Spent Engine by Microorganisms

2018 ◽  
Author(s):  
A. A. Ayandele
Keyword(s):  
Organic Matter ◽  
Contaminated Soil ◽  
Heterotrophic Bacteria ◽  
Ph Value ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Degrading Bacteria ◽  
Spent Engine Oil ◽  
Test Organisms

AbstractThe potential of six microorganisms (Pseudomonas aeruginosa, Micrococcus sp, Flavobacterium sp, Rhizopus sp, Penicillium sp and Fusarium sp) isolated from hydrocarbon contaminated site were evaluated for their biodegradation ability. The soil samples were contaminated with 5% (w/v) of spent engine oil and the rate of biodegradation of the oil was studied for a period of 10weeks under greenhouse experiment. The total heterotrophic bacteria count (THBC), total hydrocarbon degrading bacteria count (THDBC), physicochemical and heavy metals properties of the soil samples and Total Petroleum Hydrocarbon (TPH) were determined after treatment with test organisms. THBC and THDBC ranged from 0.175 to 0.280 CFUg-1 and 0.47 CFUg-1 respectively for the control plot, while THBC is ranging from 0.197 to 0.275 CFUg-1 and THDBC was 0.180 to 0. 473 CFUg-1 for the contaminated plot. There was a slight increase in the pH value of the contaminated soil sample and the treated soil samples as the experimental weeks increased. The results obtained showed a significant decrease (at p ≤ 0.05) in the nutrients content of the soil samples. There was an increase from 1.09 in the control to 15.5% in the content of organic matter after contamination and from 1.88% to 26.8% in the % of organic matter too. There was a significant reduction (at p ≤ 0.05) in the concentration of Fe, Zn, Pb, Cd, Cu, Cr and Ni after 10 weeks of incubation with the tested organisms. Plant growth in the treated contaminated soil samples ranged from 32.6cm to 38.6cm, while that of the control 1 (Uncontaminated soil) was 51.2cm and 19.7cm high was observed in the Control 2 (contaminated untreated soil) after 22 days of the experiment. The TPH degradation (% loss) ranged from 79.7 to 89. 2% after 10 weeks of treatment. P. aeruginosa had the highest level of degradation (89.2%), while Micrococcus sp and Rhizopus sp had the least degradation at 79.9%.All the microorganisms used in this study had the abilities to remediating soil contaminated with spent engine oil and the remediated soil samples were able to support the growth of Zea mays at 5% (w/v) level of contamination.

Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State

2021 ◽  
Vol 4 (2) ◽  
pp. 35-38
Author(s):  
Hussaini Shettima ◽  
Ibrahim Alkali Allamin ◽  
Nasir Halima ◽  
Haruna Yahya Ismail ◽  
Yusuf Musa
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Bacillus Coagulans ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Hydrocarbon Utilizing Bacteria

The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road, and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.

Gravimetric Profile of Hydrocarbon Degrading Bacterial and Fungal Isolates from Contaminated Soil Samples in Ado-Ekiti, Nigeria

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Olayinka O. Idris ◽  
◽  
Olayinka T. Ogunmefun ◽  
Cinderella N. Tuesimi
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Soil Samples ◽  
Engine Oil ◽  
Water Retention Capacity ◽  
Trichoderma Spp ◽  
Fungal Isolates ◽  
Degrading Bacteria ◽  
Spent Engine Oil

One of the biological compounds limiting soil water retention capacity is oil when present due to its hydro-nature. However, some microorganisms exhibit the capacity to degrade oil as a source of carbon, whereby the soil quality is retained and enhanced. Hence, the gravimetric profile of hydrocarbon degrading bacteria and fungi isolated from oil contaminated soil samples was investigated. Soil samples were collected from surface and 10m depth from six different mechanic workshops and generator sites. The pour plate technique was used to isolate the microorganisms. All pure isolates were sub-cultured using Bushnell Haas agar and the isolated bacteria were identified by their morphological and biochemical characteristics. The soil samples pH range was 4.3 - 6.4. Bacteria isolated included Pseudomonas spp., Staphylococcus spp., Microccocus spp., Acinetobacter spp., and Bacillus spp. The fungi isolated included Aspergillus spp., Rhizopus spp., Candida spp., Trichoderma spp. and Penicillium spp. Degradation of kerosene, diesel, crude oil, engine oil, and spent engine oil was allowed using Acinetobacter baumanni, P. aeruginosa, B. subtilis, and S. aureus. Gravimetric analyses were used to determine the percentage of petroleum hydrocarbon degraded by bacterial isolates. The highest percentage of degradation was between P. aeruginosa and B. subtilis. Pseudomonas aeruginosa degraded 97.4% diesel, 88.2% kerosene, 71.3% crude oil, 80.7% engine oil and 78.2% spent engine oil; while Bacillus subtilis degraded 71% diesel, 97% kerosene, 89.6% crude oil, 87% engine oil and 72.6% spent engine oil. This study revealed that bacterial and fungal isolates from oil contaminated soils exhibited the potentials to degrade oil and bioremediation using these microorganisms was possible.

scholarly journals Phytoremediation of Spent Oil and Palm Kernel Sludge Contaminated Soil Using Sunflower (Helianthus annuus) L

2021 ◽  
Vol 25 (5) ◽  
pp. 877-885
Author(s):  
A.J. Odebode ◽  
K.L. Njoku ◽  
A.A. Adesuyi ◽  
M.O. Akinola
Keyword(s):  
Helianthus Annuus ◽  
Plant Height ◽  
Palm Oil ◽  
Contaminated Soil ◽  
Palm Kernel ◽  
Engine Oil ◽  
Palm Kernel Oil ◽  
Kernel Oil ◽  
Spent Engine Oil ◽  
And Control

This study was carried out to investigate the phytotoxicity of spent engine oil and palm kernel sludge on seed germination, seedling early growth and survival of sunflower (Helianthus annuus L) and its phytoremediating potential. 8.0 kg topsoil mixed with 2, 4, 6, 8 and 10% (w/v) of spent engine oil and palm kernel sludge, while the control was not mixed with spent oil and sludge (0%). The seeds were sown on these soils and monitored daily. Parameters taken were; plant height, leaf number and stem girth. The result showed that spent engine oil treated plants adversely affected growth compared to palm kernel sludge plants and control which performed better. For plant height, the mean stem girth for control at 2nd week was 0.40±0.05 mm, spent engine oil was 5.96±0.97 palm kernel oil effluent was 14.73±1.16 and at 12th week, control was 1.30±0.05 while for SEO the plant had withered and 124.6±9.02 for POE. Number of leaves at the 12th week was 26.00±2.08 in the control, 8.66±0.66, for spent engine oil at 4%, while for palm oil effluent it was 27.66±0.66, at 4%, concentration respectively. Stem girth at 2 weeks for spent engine oil was 0.19±0.05 at 2%, 0.43±0.03 for palm kernel oil effluent and at the 12th week of planting at 10% concentration was 1.63±0.08 for palm kernel oil effluent, and all plants had withered off for spent engine oil at same concentration at the 12th week. Also, spent engine oil at all concentrations delayed the germination of Helianthus annuus by 2days compared to control. Comparison analysis test showed that growth in untreated plants were significantly higher (p>0.05) than spent oil and palm kernel sludge treated plants. Similar result was observed for leaf number and stem girth which had higher mean value in palm kernel sludge and control compared to spent oil. Sunflower grown in 8% and 10% palm kernel sludge contaminated soil also flowered eight days earlier than control plants, while spent oil treated plant did not. The result shows that sunflower cannot tolerate high (4%, 6%, 8% and 10%) concentrations of spent engine oil in soil compared to palm oil effluent. Therefore, spent engine oil should be properly disposed because of its adverse effect on the growth and yield of sunflower.

scholarly journals Phenylobacterium Korensee Best Indigenous Petroleum Hydrocarbon Degrading Bacteria Isolated from Contaminated Soil of Bahror, Alwar Region, India

2019 ◽  
Vol 10 (08) ◽  
pp. 20203-20211 ◽  
Author(s):  
Mohan Lal Kuri ◽  
Vidhya Kumari ◽  
Shikha Roy
Keyword(s):  
Contaminated Soil ◽  
Engine Oil ◽  
Contaminated Site ◽  
Spectroscopy Analysis ◽  
Global Issue ◽  
Degrading Bacteria ◽  
Term Basis ◽  
Agar Media ◽  
Gas Chromatography Mass Spectroscopy

Contamination of soil, water and air due to hydrocarbons are a global issue and bioremediation provides probably the best way to remediate the contaminants. The current study shows the biodegradation of crude oil, diesel and used engine oil by a newly isolated Phenylobacterium korensee from contaminated soil of Bahror, Alwar, Rajasthan. Hydrocarbon degrading strain was screened on BHA (Bushnell Haas Agar) media supplemented with 2T engine oil as sole carbon source. The strain was found to be degrading at 1%, 4% and 10% of used 2T engine oil respectively after 14 days. Degradation was confirmed both gravimetrically and by Gas Chromatography Mass Spectroscopy analysis. The degradation was found very well at long term basis. The optimization of growth also studied at temperature and pH basis also. The significance of the study is that the percentage degradation of the complex petroleum supplements used in the study was found to be far higher than some of the previously reported values and this bacterial strain was firstly found from this contaminated site.

scholarly journals BIO STIMULATORY INFLUENCE OF COMPOST AND INORGANIC FERTILIZER ON BACTERIAL DEGRADATION OF SPENT ENGINE OIL CONTAMINATED SOIL

2020 ◽  
Vol 4 (3) ◽  
pp. 624-630
Author(s):  
A. Adeleye ◽  
Victor O. Onokebhagbe ◽  
A. Akindiya ◽  
M. E. Nkereuwem
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Inorganic Fertilizer ◽  
Engine Oil ◽  
Bacterial Degradation ◽  
Incubation Study ◽  
Study Results ◽  
Spent Engine Oil ◽  
Hydrocarbon Utilizing Bacteria

The harmful effects of spent engine oil on aquatic and terrestrial ecosystems have been well established in literature. This study was conducted to assess the bio stimulatory influence of amendments; compost and inorganic fertilizer on bacterial degradation of spent engine oil contaminated soil.  500 g of unpolluted soil was collected and artificially contaminated with 5, 10 and 15% (v/w) spent engine oil. The experiment was laid out in a completely randomized design. Each amendment (50 g) was subsequently mixed thoroughly with the spent engine oil contaminated soil at varying levels except nine experimental bottles used as control. Incubation study was carried out and lasted for four weeks. Estimation of total petroleum hydrocarbon, pH, electrical conductivity, temperature and bacterial population density was done within two weeks interval during the incubation study. Results obtained indicate that compost significantly enhanced total petroleum hydrocarbon reduction by 87% while inorganic fertilizer facilitated 62% total petroleum hydrocarbon reduction on 5% spent engine oil contamination level respectively. Estimation of hydrocarbon utilizing bacteria on 5, 10 and 15% spent engine oil contaminated soil indicated that compost enhanced higher hydrocarbon utilizing bacteria thereby influencing bacterial degradation than those stimulated with inorganic fertilizer. pH remained in the range of alkalinity (7.8 and 8.06) for compost and 6.2 and 6.7 for inorganic fertilizer. The potential that compost recorded in influencing bacterial degradation of spent engine oil contaminated soil in this study has made it a good bio stimulant for the bioremediation of hydrocarbon polluted environments.

scholarly journals Characterization of hydrocarbon utilizing bacteria in waste engine oil-impacted sites

2020 ◽  
Author(s):  
Iniobong Ime James ◽  
Mayen Godwin Ben ◽  
Agnes Monday Jones ◽  
Patience Saturday Akpan ◽  
Idorenyin Idorenyin Eka ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Oil Pollution ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Engine Oil ◽  
Soil Physicochemical Properties ◽  
Selective Enrichment ◽  
Waste Engine Oil ◽  
Enrichment Technique ◽  
Degrading Bacteria

Changes in soil physicochemical properties and bacterial species present in soil contaminated with waste engine oil were evaluated at three auto-mechanical workshops in Uyo, Nigeria. This work was aimed at isolating and identifying hydrocarbon degrading bacteria from waste engine oil polluted soil, and assessing their hydrocarbon-utilizing ability. Waste engine oil pollution affected soils significantly with increases in soil physicochemical properties, and heterotrophic bacterial population counts. Eight bacterial species Corynebacterium kutscheri, Pseudomonas aeruginosa, Flavobacterium aquatile, Serratia odorifera, Micrococcus agilis, Staphylococcus aureus, Micrococcus luteus and Bacillus substilis were isolated by the selective enrichment technique and screened for hydrocarbon utilization capability in mineral salt media with 1% (v/v) waste engine oil as a sole carbon and energy source. The extent of bacterial growth observed was related to the ability of organisms to biodegrade hydrocarbons present in the medium bacterium species, which showed varying hydrocarbon utilization during the 15 days of incubation. Growth in hydrocarbon medium was the most efficient in cultures of Corynebacterium kutscheri. All isolates also showed variable emulsification ability, with Corynebacterium kutscheri, showing the highest ability. These results demonstrate the presence of indigenous bacteria in hydrocarbon-polluted soils and the potential toward the remediation of hydrocarbons.

scholarly journals Isolation, Characterization and Bioremediation Potentials of Spent Engine Oil Degrading Fungi from Contaminated Soil

2018 ◽  
Vol 4 (1) ◽  
pp. 253-255
Author(s):  
D.A. Duniya ◽  
D.B. Maikaje ◽  
Y.A. Umar ◽  
Daniel Abba ◽  
P. Omokunmi
Keyword(s):  
Contaminated Soil ◽  
Engine Oil ◽  
Spent Engine Oil
Sign in / Sign up

Export Citation Format

Share Document