Effect of seed treatment using Mancozeb and Ridomil fungicides on Rhizobium strain performance, nodulation and yield of soybean (Glycine max L.)

2021 ◽  
Vol 4 (2) ◽  
pp. 86-97
Author(s):  
Zerihun Getachew ◽  
Lejalem Abeble

The viability of commercial Rhizobium strains (SB-14 and SB-12) were inoculated and fungicides (Mancozeb and Ridomil) were used as seed dressed on soybean seed to investigate their effect on nodulation, plant growth and seed yield of soybean. Application of Rhizobial inoculants alone gave the highest nodulation and shoot dry weight performance as well as seed yield of soybean on both sites. SB-12 inoculant had significantly shown to be more effective than SB-14 inoculant in increasing nodulation and thus produced higher plant growth and seed yield. Rhizobial survival on the seeds was severely affected by both fungicides, resulting in decreased nodulation, plant growth and seed yield for both inoculants. However, Ridomil fungicide gave the lowest nodulation and seed yield when applied with either SB-12 or SB-14 Rhizobial strains. The strains differed in their sensitivity to Mancozeb fungicide that with strain SB-12 showed a slight effect or no effect on survival of rhizobium, nodulation and yield of soybean. Seed-dressing of mancozeb and ridomil resulted in reduction of seed yield by 882.8 kg ha-1 and 1154.7 kg ha-1, respectively with SB-12 strain. The present results indicate that inoculated Rhizobium inoculants differ in their capacity to develop resistance to the two dressed fungicides. Seed treatment with Mancozeb in combination with SB-12 strain slightly affected the survival of the inoculated strain. Consequently, mancozeb fungicide may be compatable with survival of the inoculated SB-12 Rhizobia. The results also indicate that the suppressive effects of seed-applied fungicides on Rhizobium strains survival and nodulation development depend on specific strain and fungicide. Soybean seeds inoculated with SB-12 may not need management with fungicides or lower concentration of Mancozeb that could be compatible with SB-12 to suppress soil-borne pathogens for both Assosa and Begi sites, western Ethiopia.

2020 ◽  
Vol 27 (2) ◽  
pp. 251-266
Author(s):  
Muhammad Ehsan Safdar ◽  
Muhammad Ather Nadeem ◽  
Abdul Rehman ◽  
Amjed Ali ◽  
Nasir Iqbal ◽  
...  

Little is known about best herbicidal weed option for weed eradication in soybean in agro-climatic circumstances of Sargodha, Punjab, Pakistan. A two year field study was accomplished at College of Agriculture experimental site Sargodha in spring seasons of 2018 and 2019 to evaluate the efficacy of different herbicides adjacent to major weeds present in soybean. The study consisted of 8 herbicide treatments including two pre-emergence herbicides (pendimethalin at 489.1 g a.i. ha-1, pendimethalin + S-metolachlor at 731.1 g a.i. ha-1) which are applied immediately after sowing and six post-emergence herbicides (oxyfluorfen at 237.1 g a.i. ha-1, metribuzin at 518.7 g a.i. ha-1, quizalofop-p-ethyl at 148.2 g a.i. ha-1, acetochlor at 741 g a.i. ha-1, halosulfuron at 37 g a.i. ha-1and topramezone at 21.5 g a.iha-1) which were used 25 days subsequent to sowing. In contrast to control, all herbicides have shown significant decline in weed density (up to 94%) and dry weight (up to 88%); and caused significant increases in plant height (up to 85%), pod bearing branches (up to 77%), number of pods per plant (up to 83%), 100-seed weight (up to 37%) and seed yield (up to 160%) of soybean. Among herbicides, topramezone at 21.5 g a.i ha-1 gave significantly the highest (1234 and 1272 kg ha-1 in the year 2018 and 2019) seed yield of soybean and HEIs (1.28 and 1.03 in year 2018 and 2019, respectively). However, oxyfluorfen at 237.1 g a.i. ha-1, pendimethalin + S-metolachlor at 731.1 g a.i. ha-1, pendimethalin at 489.1 g a.i. ha-1, quizalofop-p-ethyl at 148.2 g a.i.ha-1 followed it. The regression analysis depicted a significant negative moderate relationship of soybean seed yield with weed dry weight (R2 = 0.7074), and pods per plant (R2 = 0.7012) was proved to be the main yield component responsible for higher yield of soybean.


2009 ◽  
Vol 89 (2) ◽  
pp. 265-272
Author(s):  
L. R. Brown ◽  
D. E. Robinson ◽  
K. Chandler ◽  
C. J. Swanton ◽  
R. E. Nurse ◽  
...  

There have been anecdotal accounts of increased crop sensitivity due to herbicide drift followed by an in-crop herbicide. An experiment was conducted from 2005 to 2007 at Elora, Ridgetown, and Woodstock, Ontario, to determine the effects of simulated mesotrione drift followed by in-crop applications of glyphosate, imazethapyr, bentazon and glyphosate plus chlorimuron on glyphosate-resistant soybean [Glycine max (L.) Merr.] visual injury, plant height, plant density, shoot dry weight, and seed yield. As the rate of simulated mesotrione drift increased, there was an increase in soybean injury and a decrease in shoot dry weight, height, and yield. Simulated mesotrione drift followed by bentazon resulted in synergistic responses in injury shortly after application in some environments. This increase in injury was transient, with no synergistic responses in density, shoot dry weight, and yield. In contrast, antagonistic responses were observed when glyphosate, imazethapyr, or glyphosate plus chlorimuron were applied after simulated mesotrione drift in some environments. Further research is required to develop a better understanding of the interactions of drift followed by the application of an in-crop herbicide. Key words: Bentazon, chlorimuron, glyphosate, imazethapyr, mesotrione, synergism


2015 ◽  
Vol 68 ◽  
pp. 160-165
Author(s):  
D.R.W. Kandula ◽  
H. Alizadeh ◽  
C.S.P. Teixiera ◽  
D. Gale ◽  
A. Stewart ◽  
...  

Camelina sativa seedling emergence and plant growth can be reduced by the soilborne pathogen Rhizoctonia solani The effect of a mixture of Trichoderma atroviride isolates applied in granule and prill formulations at a rate equivalent to 15 kg/ha on two varieties of C sativa (Suneson and 4164) was evaluated in glasshouse experiments using R solani infested soil The bioinoculant treatments significantly increased seedling emergence by 3060 and increased total shoot and root dry weight by 48 fold over that of the untreated control In a field experiment in 20122013 using C sativa variety 4164 the prill formulation of the bioinoculant was applied at sowing Seedling emergence was not significantly increased but shoot dry weight was increased by 20 and seed yield was increased by 25 by the bioinoculant treatment over that of the control Trichoderma bioinoculants may have a role in the integrated crop management of this second generation biofuel crop


2020 ◽  
Vol 40 (01) ◽  
Author(s):  
N. Hangsing ◽  
L. Tzudir ◽  
A. P. Singh

The field experiment conducted in the experimental farm of School of Agricultural Sciences and Rural Development (SASRD), Nagaland University during kharif season (June- September) of 2016 revealed that spacing at 30 x 10 cm recorded significantly the higher plant height (48.10 cm), number of leaves (5.47), shoot dry weight (2.88 g plant-1), LAI (2.88) and yield attributes such as number of pods plant-1 (17.00), length of pods (6.81 cm), seed yield (688.54 kg ha-1) and stover yield (1917.81 kg ha-1) respectively. Application of phosphorus at 40 kg ha-1 recorded significantly the higher plant height (49.37 cm) , number of leaves (5.73), shoot dry weight (3.34 g plant-1) , LAI (3.34) , CGR (6.37 g m-2 day-1) and yield attributes such as number of pods plant-1 (17.56) , length of pods (6.91 cm), seed yield (737.42 kg ha-1) and stover yield (1973.01 kg ha-1) respectively.


Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 574
Author(s):  
Yun-Yin Feng ◽  
Jin He ◽  
Yi Jin ◽  
Feng-Min Li

Both water stress and P deficit limit soybean seed yield, but the effects of water regimes and P application rates, their interaction on P status, acquisition, and partitioning, and their roles in yield performance have not been well-studied. Two soybean genotypes (Huangsedadou (HD) and Zhonghuang 30 (ZH)) with contrasting seed yield and root dry weight (DW) were used to investigate the P status, P acquisition, P partitioning, and yield formation under two water regimes (well-watered (WW) and cyclic water stress (WS)) and three P rates (0 (P0), 60 (P60), and 120 (P120) mg P kg−1 dry soil). The results show that increased P and water supply increased the seed yield, shoot and root DW and P concentrations and accumulations in different organs. Cultivar ZH had a significantly higher seed yield than HD at P60 and P120 under WS and at P0 under WW, but a lower seed yield at P60 and P120 under WW. Cultivar ZH had a significantly higher P harvest index and P acquisition efficiency, but a significantly lower shoot and root DW than HD. The interaction between water treatments and P rates had significant effects on leaf and stem P concentration. Cultivar ZH had significantly lower P partitioning to leaves and stems but significantly higher P partitioning to seeds than HD. The seed yield was positively correlated with leaf and seed P accumulations and P acquisition efficiency under WS. We conclude that (1) adequate water supply improved the P mobilization from leaves and stems at maturity, which may have improved the seed yield; and (2) the high P acquisition efficiency is coordination to high P partition to seeds to produce a high seed yield under water- and P-limited conditions.


Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.


Weed Science ◽  
1990 ◽  
Vol 38 (2) ◽  
pp. 113-118 ◽  
Author(s):  
S. Kent Harrison

Multiple regression and response surface plots were used to analyze the effects of common lambsquarters population density and interference duration on weed growth and soybean seed yield. Under favorable growing conditions in 1986, weed biomass production at all population densities and interference durations was four to five times that produced in 1987, under less favorable conditions. However, there was no significant treatment by year interaction for soybean seed yield reduction by common lambsquarters, and production of each kg/ha weed biomass resulted in an average soybean yield reduction of 0.26 kg/ha. Utilizing 5% yield loss as an arbitrary threshold level, the regression equation predicted a common lambsquarters density threshold of 2 plants/m of row for 5 weeks of interference after crop emergence and 1 plant/m of row for 7 weeks. Seed production by individual common lambsquarters plants was highly correlated (r=0.92) with weed dry weight, and seed production ranged from 30 000 to 176 000 seeds/plant.


2021 ◽  
Vol 11 (01) ◽  
pp. 736-748
Author(s):  
M. M. Hossain ◽  
◽  
S. Yesmin ◽  
M. Z. Islam ◽  
M. A. Hossain ◽  
...  

An experiment was conducted at the Sher-e-Bangla Agricultural University farm from April to June 2017 in Kharif season to evaluate the performance of two mungbean varieties under different NPK fertilizers doses in field conditions. The experiment was laid out in Randomized Complete Block Design (RCBD), comprising six treatments with three replications. In the experiment, varieties used were BARI mung-6 and BARI mung-5 and the combination of the treatment were T0 = (control), T1 (10-40-25 NPK kg ha-1), T2 (10-60-45 NPK kg ha-1), T3 (20-50-35 NPK kg ha-1), T4 (30-40-25 NPK kg ha-1) and T5 (30-40-45 NPK kg ha-1), respectively. Mungbean cultivars responded noticeably to the supplementary NPK fertilizers as the crop characters were significantly influenced by different levels of NPK fertilizers. Data were recorded on plant height, leaves plant-1, branches plant-1, pods plant-1, seeds pod-1, 1000-seed weight, seed, and straw yield at different days after sowing. Of the two varieties, BARI mung-6 gave the highest seed yield (1.72 t ha-1) next to the higher plant growth and straw yield On the contrary, BARI mung-5 cultivar produced the lowest seed yield (1.57t ha-1), plant growth, and straw yield. The results showed that T3 (20-50-35 NPK kg ha-1) treatment was the best treatment as regards plant growth and yield parameters. The highest seed yield was produced by treated plot T3 (20-50-35 NPK kg ha-1) over the untreated control plot, T1, T2, T4, and T5, respectively. As for the combined effect, V1T3 (BARI mung-6 and T3 = 20kg N + 50kg P + 35kg K ha-1) performed the best results in all growth and development characters. Therefore, the combined application of 20-50-35 kg NPK ha-1 might be considered to be found optimal to get a considerable seed yield of mungbean variety BARI mung-6.


2021 ◽  
Author(s):  
Leila Tabande ◽  
Mozhgan Sepehri ◽  
Jafar Yasrebi ◽  
Mehdi Zarei ◽  
Reza Ghasemi-Fasaei ◽  
...  

Abstract Zinc oxide nanoparticles (ZnO-NPs) are among the most commonly used nano-fertilizers (NF). However, elevated levels of ZnO-NPs in soil may affect plant growth and development due to its potential toxicity when accumulated in large amounts in plant tissues. This research was conducted using an in situ rhizobox system with the aims of evaluating Zinc uptake from nano-zinc oxide amended rhizosphere soil by alfalfa plant and the effect of plant growth promoting microorganisms on alleviating the phytotoxicity of ZnO-NPs. Treatments included microbial inoculations (Sinorhizobium meliloti, Serendipita indica) and different ZnO-NPs concentrations (0, 400 and 800 mg Kg− 1) with three replications. The results indicated that S. indica minimized the phytotoxicity of ZnO-NPs to alfalfa by enhancing growth rate and decreasing Zinc (Zn) translocation from root to shoot. Compared with plants inoculated with S. meliloti, co-inoculation with S. indica increased the shoot dry weight by 18.33% and 8.05% at 400 and 800 mg Kg− 1ZnO-NPs. However, at the highest level of ZnO-NPs (800 mg kg− 1), root inoculation of S. indica and S. indica + S. meliloti decreased Zn transfer factor by 60.2% and 44.3% compared to S. meliloti, respectively. Furthermore, a distinct relation between tolerance of S. indica-colonized plant to ZnO-NPs and the ability of S. indica in inhibiting or retarding degradation of polyunsaturated lipids through prevention of excess reactive oxygen species formation was observed. Malondialdehyde content of inoculated plants with S. indica either alone or in combination with S. meliloti was significantly lower than non-inoculated plants (p < 0.01). Zn-induced oxidative stress was mitigated by S. indica through enhanced activities of catalase and peroxidase enzymes. The findings of the present study indicate the potential use of endophytes fungus S. indica for ensuring food safety and security, and human health in heavy metal–polluted soil by reducing the phytoavailability of heavy metals in the aerial parts of the host plants.


2020 ◽  
Vol 4 (1) ◽  
pp. 229-238
Author(s):  
Dayang Rahmanita Simanjuntak ◽  
Halimursyadah Halimursyadah ◽  
Syamsuddin Syamsuddin

Abstrak. Biological seed treatment merupakan salah satu perlakuan benih menggunakan mikroorganisme seperti rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian ini bertujuan untuk mengetahui jenis rizobakteri dan kerapatan inokulum yang dapat meningkatkan viabilitas dan vigor benih cabai kadaluarsa. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor dan 3 ulangan. Faktor pertama adalah jenis rizobakteri (R) terdiri atas lima taraf yaitu R1: Necercia sp; R2:Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. Faktor kedua adalah kerapatan inokulum rizobakteri terdiri dari tiga taraf yaitu K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. Hasil penelitian ini menunjukkan bahwa perlakuan benih menggunakan rizobakteri jenis Necercia sp dengan kerapatan inokulum 108 cfu/ml nyata meningkatkan vigor benih pada tolok ukur  indeks vigor yaitu 40% dan Pseudomonas capacia dengan kerapatan inokulum 109 cfu/ml juga merupakan kombinasi perlakuan terbaik dalam meningkatkan berat kering kecambah normal yaitu 69,33 mg.Treatment Of Plant Growth Promoting Rhizobacteria (PGPR)With Multiple Levels of Rhizobacteria Inoculum Density On Viability and Vigor Of Expired Red Chilli Seeds (Capsicum annuum L.Abstract. Biological seed treatment is one of the seed treatment using microorganisms such as plant growth-promoting rhizobacteria (PGPR). This study aims to determine the type of rhizobacteria and inoculum density that can increase the viability and vigor of expired chili seeds. This research uses Completely Randomized Design (CRD) factorial pattern with 2 factors and 3 replications. The first factor is the type of rhizobacteria (R) consists of five levels, namely R1: Necercia sp; R2: Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. The second factor is the density of rhizobacteria inoculum consisting of three levels namely K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. The results of this study showed that the seed treatment using the Necercia sp-type rizobacteria with 108 cfu/ml inoculum density significantly increased the seed vigor on the vigor index benchmark of 40% and Pseudomonas capacia with 109cfu/ml inoculum density was also the best treatment combination in increasing dry weight normal sprout is 69,33 mg. 


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