Incidence and antibiotic susceptibility pattern of Escherichia coli and Staphylococcus aureus isolated from meat pie sold in a Nigerian North Central town

Background and Objectives: Meat pie is a popular ready-to-eat food sold in Nigeria and is consumed by people of all classes and category. The study aimed to determine the incidence and susceptibility of Escherichia coli and Staphylococcus aureus isolated from meat pie to antibiotics commonly administered in Makurdi.Material and Methods: A total of 180 samples were collected and evaluated for bacterial contamination and presence of antibiotic-resistant Escherichia coli and Staphylococcus aureus. Contaminants were isolated and identified using biochemical test. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer disc diffusion method.Results: Eleven bacterial genera was identified. Bacillus spp (85%) occurred most frequently, followed by Staphylococcus aureus (38.9%), while Edwardsiella spp (2.8%) occured the least. Staphylococcus aureus was highly resistant to Cloxacillin (87.1%) but highly susceptible to Ofloxacin (88.6%). Escherichia coli was resistant to Amoxycillin, Tetracycline, Cloxacillin and Augmentin but susceptible to Gentamicin and Ofloxacin. Conclusion: Meat pie sold in Makurdi habours Staphylococcus aureus and Escherichia coli with multiple antibiotic resistance. Regulation of the production and retail process of meat pie is advocated as a possible means of reducing contamination and the risk of transferring antibiotic resistant bacteria to consumers.

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Antibacterial Activities of Naturally Occurring Compounds against Antibiotic-Resistant Bacillus cereus Vegetative Cells and Spores, Escherichia coli, and Staphylococcus aureus

Journal of Food Protection ◽

10.4315/0362-028x-67.8.1774 ◽

2004 ◽

Vol 67 (8) ◽

pp. 1774-1778 ◽

Cited By ~ 60

Author(s):

MENDEL FRIEDMAN ◽

ROBERT BUICK ◽

CHRISTOPHER T. ELLIOTT

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Bacillus Cereus ◽

Resistant Bacteria ◽

Vegetative Cells ◽

Cinnamon Oil ◽

Antibiotic Resistant ◽

Thyme Oil ◽

Oregano Oil ◽

And Staphylococcus Aureus

After demonstrating the lack of effectiveness of standard antibiotics against the acquired antibiotic resistance of Bacillus cereus (NCTC 10989), Escherichia coli (NCTC 1186), and Staphylococcus aureus (ATCC 12715), we showed that the following natural substances were antibacterial against these resistant pathogens: cinnamon oil, oregano oil, thyme oil, carvacrol, (S)-perillaldehyde, 3,4-dihydroxybenzoic acid (β-resorcylic acid), and 3,4-dihydroxyphenethylamine (dopamine). Exposure of the three pathogens to a dilution series of the test compounds showed that oregano oil was the most active substance. The oils and pure compounds exhibited exceptional activity against B. cereus vegetative cells, with oregano oil being active at nanogram per milliliter levels. In contrast, activities against B. cereus spores were very low. Activities of the test compounds were in the following approximate order: oregano oil > thyme oil ≈ carvacrol > cinnamon oil > perillaldehyde > dopamine>β-resorcylic acid. The order of susceptibilities of the pathogens to inactivation was as follows: B. cereus (vegetative) ≫S. aureus ≈ E. coli ≫ B. cereus (spores). Some of the test substances may be effective against antibiotic-resistant bacteria in foods and feeds.

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Hypochlorite-Activated Fluorescence Emission and Antibacterial Activities of Imidazole Derivatives for Biological Applications

Frontiers in Chemistry ◽

10.3389/fchem.2021.713078 ◽

2021 ◽

Vol 9 ◽

Author(s):

Thanh Chung Pham ◽

Van-Nghia Nguyen ◽

Yeonghwan Choi ◽

Dongwon Kim ◽

Ok-Sang Jung ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Stokes Shift ◽

Fluorescence Emission ◽

Antibacterial Activities ◽

Resistant Bacteria ◽

Antibiotic Resistant ◽

Fluorogenic Probe ◽

High Fluorescence

The ability to detect hypochlorite (HOCl/ClO−) in vivo is of great importance to identify and visualize infection. Here, we report the use of imidazoline-2-thione (R1SR2) probes, which act to both sense ClO− and kill bacteria. The N2C=S moieties can recognize ClO− among various typical reactive oxygen species (ROS) and turn into imidazolium moieties (R1IR2) via desulfurization. This was observed through UV–vis absorption and fluorescence emission spectroscopy, with a high fluorescence emission quantum yield (ՓF = 43–99%) and large Stokes shift (∆v∼115 nm). Furthermore, the DIM probe, which was prepared by treating the DSM probe with ClO−, also displayed antibacterial efficacy toward not only Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) but also methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum ß-lactamase–producing Escherichia coli (ESBL-EC), that is, antibiotic-resistant bacteria. These results suggest that the DSM probe has great potential to carry out the dual roles of a fluorogenic probe and killer of bacteria.

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Sensitivity of antibiotic resistant and antibiotic susceptible Escherichia coli, Enterococcus and Staphylococcus strains against ozone

Journal of Water and Health ◽

10.2166/wh.2015.291 ◽

2015 ◽

Vol 13 (4) ◽

pp. 1020-1028 ◽

Cited By ~ 6

Author(s):

Stefanie Heß ◽

Claudia Gallert

Keyword(s):

Escherichia Coli ◽

Biofilm Formation ◽

Resistance Pattern ◽

Resistant Bacteria ◽

Antibiotic Resistant ◽

E Coli ◽

Enterococcus Casseliflavus ◽

Subsurface Layers ◽

Wastewater Samples ◽

And Staphylococcus Aureus

Tolerance of antibiotic susceptible and antibiotic resistant Escherichia coli, Enterococcus and Staphylococcus strains from clinical and wastewater samples against ozone was tested to investigate if ozone, a strong oxidant applied for advanced wastewater treatment, will affect the release of antibiotic resistant bacteria into the aquatic environment. For this purpose, the resistance pattern against antibiotics of the mentioned isolates and their survival after exposure to 4 mg/L ozone was determined. Antibiotic resistance (AR) of the isolates was not correlating with higher tolerance against ozone. Except for ampicillin resistant E. coli strains, which showed a trend towards increased resistance, E. coli strains that were also resistant against cotrimoxazol, ciprofloxacin or a combination of the three antibiotics were similarly or less resistant against ozone than antibiotic sensitive strains. Pigment-producing Enterococcus casseliflavus and Staphylococcus aureus seemed to be more resistant against ozone than non-pigmented species of these genera. Furthermore, aggregation or biofilm formation apparently protected bacteria in subsurface layers from inactivation by ozone. The relatively large variance of tolerance against ozone may indicate that resistance to ozone inactivation most probably depends on several factors, where AR, if at all, does not play a major role.

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Laboratory-based Surveillance of Clinical Isolates of Antibiotic-resistant Bacteria and Related Risk Factors-Extended-spectrum .BETA.-lactamase-producing Escherichia coli and Methicillin-resistant Staphylococcus aureus-

Iryo Yakugaku (Japanese Journal of Pharmaceutical Health Care and Sciences) ◽

10.5649/jjphcs.36.648 ◽

2010 ◽

Vol 36 (9) ◽

pp. 648-656

Author(s):

Takako Imazeki ◽

Yoko Kiyota ◽

Yoshiko Watanabe ◽

Norio Nishiyama ◽

Kazunori Ozeki ◽

...

Keyword(s):

Risk Factors ◽

Escherichia Coli ◽

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Resistant Bacteria ◽

Beta Lactamase ◽

Antibiotic Resistant ◽

Methicillin Resistant ◽

Extended Spectrum Beta Lactamase ◽

Related Risk

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Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2020.v32.i11.2190 ◽

2020 ◽

pp. 795

Author(s):

Rania Benjamaa ◽

Abdelkarim Moujanni ◽

Anass Terrab ◽

Rabiaa Eddoha ◽

Maryam Benbachir ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Health Concern ◽

Resistant Bacteria ◽

Antibiotic Residues ◽

Antibiotic Resistant ◽

Liquid Chromatography Tandem Mass

Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

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SYNTHESIS OF SILVER NANOPARTICLES FUNCTIONALIZED WITH AQUEOUS EXTRACT (Burseragraveolens) AND ANTIMICROBIAL EVALUATION IN Escherichia coli, Staphylococcus aureus AND Klebsiellapneumoniae

Periódico Tchê Química ◽

10.52571/ptq.v15.n29.2018.282_periodico29_pgs_282_291.pdf ◽

2018 ◽

Vol 15 (29) ◽

pp. 282-291

Author(s):

D. GANCHALA ◽

J. L. GU.CORONADO ◽

E. JARA ◽

L. MENESES ◽

E. GRANDA ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Infectious Diseases ◽

Aqueous Extract ◽

Antimicrobial Effect ◽

Disc Diffusion ◽

Total Inhibition ◽

Antimicrobial Evaluation ◽

And Staphylococcus Aureus

Currently, resistance to antibiotics has become a problem for the treatment of infectious diseases. In this study, silver nanoparticles were synthesized using the aqueous extract of Palo santo (Burseragraveolens), for its biocide action, in order to determine its antimicrobial effect on Escherichia coli and Staphylococcus aureus. Different concentrations of nanoparticles (100 until1000 ppm) were evaluated at contact times of 1, 2, 5 and 10 minutes for 1500 and 15000 CFU/mL. Additionally, to determine effectiveness, disc diffusion tests using various concentrations of nanoparticles (500-1000 ppm) were performed on S.aureus and K. pneumoniae. According to the results of antimicrobial effect for E.coli, the effectiveness of nanoparticles can be established at all contact times for 1500 CFU from 800 ppm and for 15000 CFU from 400 ppm. With S.aureus, total inhibition was showed from 800 ppm in 1500 CFU at 5 minutes of contact and between 500-1000 ppm with 15000 CFU at minute one. S. aureus and K.pneumoniae tests showed sensitivity with nanoparticles. The average of susceptibility for S.aureus had the lowest inhibition between 500-600 ppm and the highest at 700-800 ppm. The average susceptibility for K. pneumoniae is more constant in terms of inhibition at 600-1000 ppm. This study demonstrated the antimicrobial effect of silver nanoparticles synthesized under the specified conditions.

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Occurrence of Antibiotic Resistant Bacteria in Environmental Wastes

Our Nature ◽

10.3126/on.v7i1.2563 ◽

1970 ◽

Vol 7 (1) ◽

pp. 151-157

Author(s):

R.C. Poudel ◽

D.R. Joshi ◽

N.R. Dhakal ◽

A.B. Karki

Keyword(s):

Staphylococcus Aureus ◽

Sewage Sludge ◽

Antibiotic Susceptibility ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Multidrug Resistant Bacteria ◽

Antibiotic Resistant ◽

Antibiotic Susceptibility Pattern ◽

Antibiotic Susceptibility Test ◽

Enterococcus Spp

Microbial resistance to antibiotics has been emerging in environmental isolates. This study was carried out from October 2008 to January 2009 to describe the antibiotic susceptibility pattern of the bacteria isolated from sewage sludge, biowaste and bioslurry samples. A total of 49 identified isolates were taken for antibiotic susceptibility test. Amikacin and Gentamicin were the effective antibiotics for the Gram negative bacteria, comparatively Escherichia coli was the most sensitive. Similarly, all isolates of Staphylococcus aureus and Enterococcus spp. were sensitive to Tetracycline and Erythromycin respectively and none of these isolates were resistant to Vancomycin. Out of 13 S. aureus, five (38.5%) were Methicillin resistant Staphylococcus aureus (MRSA). The multidrug resistant (MDR) isolates accounted for 46.9%, the highest percentage of MDR isolates was seen in Pseudomonas aeruginosa (9/12, 75.0%). The proper management of multidrug resistant bacteria present in waste is suggestive for environmental and public health.Key words: Multidrug resistant bacteria, Bio-waste, Sewage sludge, BioslurryDOI: 10.3126/on.v7i1.2563Our Nature (2009) 7:151-157

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Chlorhexidine Resistance in Antibiotic-Resistant Bacteria Isolated From the Surfaces of Dispensers of Soap Containing Chlorhexidine

Infection Control and Hospital Epidemiology ◽

10.1086/501996 ◽

2002 ◽

Vol 23 (11) ◽

pp. 692-695 ◽

Cited By ~ 56

Author(s):

Steven E. Brooks ◽

Mary A. Walczak ◽

Rizwanullah Hameed ◽

Patrick Coonan

Keyword(s):

Staphylococcus Aureus ◽

Bacterial Contamination ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

Gram Negative ◽

Methicillin Resistant

AbstractBacterial contamination with pan-resistant Acinetobacter and Klebsiella, multidrug-resistant Pseudomonas, and methicillin-resistant Staphylococcus aureus (MRSA) was noted on the surfaces of dispensers of hand soap with 2% chlorhexidine. Gram-negative isolates could multiply in the presence of 1% chlorhexidine. In contrast, MRSA was inhibited in vitro by chlorhexidine at concentrations as low as 0.0019%.

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