scholarly journals Protective Effects of N. sativa against the Adverse Effects of Aflatoxicosis in Ducklings

2014 ◽  
Vol 14 (2) ◽  
pp. 81-90
Author(s):  
SKL Karn
Keyword(s):  
Adverse Effects ◽  
Biochemical Parameters ◽  
Feed Additive ◽  
Protective Effects ◽  
Glutathione Level ◽  
Histopathological Changes ◽  
Glutathione S Transferase ◽  
Total Proteins ◽  
Test Animal ◽  
Toxigenic Strain

Aflatoxin produced by Aspergillus flavus toxigenic strain was selected as a potent and widely distributed hepatotoxin that induces much health and economic hazards in animals and humans. A. flavus was cultivated on rice to produce the aflatoxin used in the present study. It was aimed to study the adverse effects of aflatoxin on some biochemical parameters in serum and liver of ducklings as well as to evaluate the possible protective effects of N.sativa crushed seeds against the adverse effects of this. Total proteins, albumin, globulins, alanine aminotransferase (ALT) (EC 2.6.1.1) and aspartate aminotransferase (AST) (EC 2.6.1.2), cholesterol and triacylglycerols were measured in the serum of the test animal. The levels of hepatic malondialdhyde and glutathione level, glutathione S-transferase (GST) (EC: 2.5.1.18) activity, aflatoxin residues and histopathological changes were also measured. The obtained results suggest that the addition of N. sativa to duckling’s ration has a protective effect against aflatoxicosis. The result concluded that aflatoxin had hepatotoxic effects through decrease of total proteins, albumin, glutathione and glutathione S-transferase. In addition, aflatoxin induced histopathological changes of liver of the test animal and residues of aflatoxin were measured. So, we advice to use N. sativa as a feed additive for controlling aflatoxicosis in poultry farm   DOI: http://dx.doi.org/10.3126/njst.v14i2.10419   Nepal Journal of Science and Technology Vol. 14, No. 2 (2013) 81-90  

scholarly journals S-Methyl Cysteine Protective Effects in Oreochromis Niloticus Fish Contaminated by Thiobencarb Herbicide

2020 ◽  
Author(s):  
Mostafa Ali Elmadawy ◽  
Walied Abdo ◽  
Amira Alaa El-Dein Omar ◽  
Nadia B. Mahfouz
Keyword(s):  
Adverse Effects ◽  
Histopathological Examination ◽  
Genotoxic Effect ◽  
Control Group ◽  
Protective Effects ◽  
Glutathione S Transferase ◽  
Commercial Feed ◽  
Significant Difference ◽  
Treated Fish

Thiobencarb which is a carbamate herbicide is used for managing undesirable weeds during rice cultivation in Egypt. This study was designed to investigate the adverse effects of a field dose of thiobencarb on Nile tilapia and ameliorating the role of the low dose of S-methyl cysteine (SMC). Experimental fishes were divided into four groups; first group was reared without any treatments and served as a control group; the second group was exposed to thiobencarb (36µg/L); the third group was fed on a commercial feed containing 200 mg of SMC/Kg in conjunction with thiobencarb added to aquarium (36µg/L) while, the fourth group was fed on a feed containing 200 mg of SMC/Kg only. Fishes were sacrificed at the end of the experimental course (two months) and sampling was carried out. Catalase, Glutathione S Transferase activities, Glutathione reduced, and Malondialdhyde levels were assayed. Genotoxic effect of thiobencarb and SMC on treated fish was investigated in erythrocytes, gills, and liver tissues using micronucleus and comet assay. Histopathological examination of livers, gills, and brain was also carried out. The results indicated that fish exposed to thiobencarb indicated herbicide dependent oxidative stress and genotoxic effect justified by a significant difference in antioxidant biomarkers as well as nuclear abnormalities and comet parameters compared to control values. Moreover, histopathological findings were in line with other results. SMC ameliorated the adverse effects which were effective in the improvement of DNA and oxidative damage in thiobencarb intoxicated fish.

scholarly journals Insights into the ecotoxicological perturbations induced by the biocide abamectin in the white snail, Theba pisana

2021 ◽  
Author(s):  
Mohamed Radwan ◽  
Amira Gad
Keyword(s):  
Biochemical Parameters ◽  
Glycogen Content ◽  
Land Snail ◽  
Energy Reserves ◽  
Glutathione S Transferase ◽  
Total Proteins ◽  
Time Intervals ◽  
Theba Pisana ◽  
Glutamyl Transferase ◽  
Lethal Doses

Abstract Abamectin (avermectin B1, ABM) has been widely used as a biocide in agriculture, veterinary and medicine worldwide. In the current study, we aimed to evaluate the acute toxicity and sub-lethal biochemical responses of ABM on the non-target land snail, Theba pisana. Mortality of snails increased with the dose increase, resulting 48h- LD50 value of 1.048 µg/snail. Sub-lethal effects were studied on the survivors of 20% and 60% LD50 ABM doses and the biochemical parameters were assessed for up to 7 days of exposure. The results showed a decrease in glycogen content and lipids for two sub-lethal doses after all time intervals, whereas increased the level of total proteins after exposure to 60% LD50 ABM. Overall, the tested sub-lethal doses significantly decreased the total energy reserves. ABM-exposure to snails elevated γ-Glutamyl transferase (γ-GT) and Lactate dehydrogenase (LDH) activities at all-time intervals. A significant increase of Glutathione-S-transferase (GST) activity was also recorded in snails exposed to 20% and 60% LD50 after 7 days and all time intervals, respectively. However, ABM inhibited the activity of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) after 7 days of exposure. Our investigation provides new insights into the disturbances of energy reserves and enzyme activities in T. pisana snails that can be used as useful sentinel organism. Indeed, these tested biochemical parameters of the snails are sensitive and may be used as biomarkers for assessing ABM toxicity.

scholarly journals Cyanobacteria, cyanotoxins, and their histopathological effects on fish tissues in Fehérvárcsurgó reservoir, Hungary

2021 ◽  
Vol 193 (9) ◽  
Author(s):  
Damjana Drobac Backović ◽  
Nada Tokodi ◽  
Zoran Marinović ◽  
Jelena Lujić ◽  
Tamara Dulić ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Fish Tissue ◽  
Freshwater Ecosystems ◽  
Abramis Brama ◽  
Histopathological Changes ◽  
Fish Tissues ◽  
The World ◽  
Encoding Gene ◽  
Microcystis Wesenbergii ◽  
Histopathological Effects

AbstractCyanobacteria are important members of lake plankton, but they have the ability to form blooms and produce cyanotoxins and thus cause a number of adverse effects. Freshwater ecosystems around the world have been investigated for the distribution of cyanobacteria and their toxins and the effects they have on the ecosystems. Similar research was performed on the Fehérvárcsurgó reservoir in Hungary during 2018. Cyanobacteria were present and blooming, and the highest abundance was recorded in July (2,822,000 cells/mL). The species present were Aphanizomenon flos-aquae, Microcystis flos-aquae, Microcystis wesenbergii, Cuspidothrix issatschenkoi, Dolichospermum flos-aquae, and Snowella litoralis. In July and September, the microcystin encoding gene mcyE and the saxitoxin encoding gene sxtG were amplified in the biomass samples. While a low concentration of microcystin-RR was found in one water sample from July, analyses of Abramis brama and Carassius gibelio caught from the reservoir did not show the presence of the investigated microcystins in the fish tissue. However, several histopathological changes, predominantly in gills and kidneys, were observed in the fish, and the damage was more severe during May and especially July, which coincides with the increase in cyanobacterial biomass during the summer months. Cyanobacteria may thus have adverse effects in this ecosystem.

scholarly journals Dietary Black Seed Effects on Growth Performance, Proximate Composition, Antioxidant and Histo-Biochemical Parameters of a Culturable Fish, Rohu (Labeo rohita)

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 48
Author(s):  
Maria Latif ◽  
Mehwish Faheem ◽  
Asmatullah ◽  
Seyed Hossein Hoseinifar ◽  
Hien Van Doan
Keyword(s):  
Growth Performance ◽  
Proximate Composition ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Labeo Rohita ◽  
Nigella Sativa ◽  
Growth Promoter ◽  
Feeding Trial ◽  
Glutathione S Transferase ◽  
Black Seed

This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa) supplementation on the growth performance, muscles proximate composition, antioxidant and histo-biochemical parameters of rohu (Labeo rohita). Fingerlings (8.503 ± 0.009 g) were fed on 0.0%, 1% and 2.5% black seed supplemented diets for 28 days. Fish sampling was done on the 7th, 14th, 21st and 28th day of experiment. The results of the present study indicated that black seed supplementation significantly increased growth performance and muscles protein contents of rohu over un-supplemented ones. Lipid peroxidation levels significantly decreased in all the studied tissues (liver, gills, kidney and brain) of black seed fed rohu, whereas the antioxidant enzymes (catalase, glutathione-S-transferase, glutathione peroxidase and reduced glutathione) activities were increased in all the studied tissues of black seed supplemented rohu at each sampling day. The hepatic-nephric marker enzymes levels were decreased for black seed fed rohu. The present study showed that tested black seed levels are safe for rohu. Black seed is cheaply available in local markets of Pakistan; therefore, based on the results of the present study, it is suggested that black seed has potential to be used as natural growth promoter and antioxidant in the diet of rohu.

Aluminium Administration is Associated with Biochemical Parameters Histopathological Changes in Rat Liver

Metabolism ◽  
2021 ◽  
Vol 116 ◽  
pp. 154505
Author(s):  
Demmouche Abbassia ◽  
H. Bekhadda ◽  
A.E. Menadi ◽  
D. Ferrag ◽  
Khalloua Zine Charaf ◽  
...  
Keyword(s):  
Rat Liver ◽  
Biochemical Parameters ◽  
Histopathological Changes

scholarly journals Effect of Humic Substances as Feed Additive on the Growth Performance, Antioxidant Status, and Health Condition of African Catfish (Clarias gariepinus, Burchell 1822)

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2266
Author(s):  
Markéta Prokešová ◽  
Milena Bušová ◽  
Mahyar Zare ◽  
Hung Quang Tran ◽  
Eliška Kučerová ◽  
...  
Keyword(s):  
Growth Performance ◽  
Humic Substances ◽  
Biochemical Parameters ◽  
Antioxidant Status ◽  
Clarias Gariepinus ◽  
Feed Additive ◽  
African Catfish ◽  
Growth Promoter ◽  
Total N ◽  
Positive Effects

In the present study, a possible nature immunostimulant and growth promoter—humic substances (HS) originating from Siberian leonardite mineraloid—were tested on juvenile Clarias gariepinus performance. Feed additive was applied onto commercial pelleted feed at four HS levels—0, 1, 3, and 6% w/w (HS0, HS1, HS3, HS6, respectively). Diets were tested in five repetitions (in total, n = 1800 individuals, mean body weight 28.1 ± 6.2 g) for 56 days. Growth and production parameters, fish condition and somatic indices, and overall mortality were evaluated after 14, 28, 42, and 56 days of exposure. Whereas, plasma samples were collected only after 0, 28, and 56 days, when fish health status was assessed with biochemical parameters (total proteins, TP; alanine aminotransferase, ALT; aspartate aminotransferase, AST; lactate dehydrogenase, LDH; cholesterol, CHOL; triglycerides, TAG) and fish antioxidant status with glutathione (reduced glutathione, GSH; oxidized glutathione, GSSG; glutathione ratio GSH/GSSG). Although a significantly positive effect of HS feed additive on growth performance was not found in the present study, moderately positive effects were found regarding biochemical parameters (ALT, AST, LDH, CHOL, and TAG) and antioxidants (GSH/GSSG ratio) that were improved especially in the HS3 group.

Women's perceptions of the safety of the pill: a survey in eight developing countries

1987 ◽  
Vol 19 (3) ◽  
pp. 313-321 ◽  
Author(s):  
Gary S. Grubb
Keyword(s):  
Developing Countries ◽  
Family Planning ◽  
Adverse Effects ◽  
Birth Defects ◽  
Health Risks ◽  
Scientific Evidence ◽  
Protective Effects ◽  
Gallup Poll ◽  
The Us ◽  
Women’S Perceptions

SummaryIn January 1985, a Gallup poll sponsored by the American College of Obstetrics and Gynecologists reported that 76% of the US women sampled thought that there were substantial risks with using the pill, 31% thought the pill caused cancer and 64% thought the risk of childbearing was equal to or less than that in taking the pill. To assess the perceptions of the pill's safety internationally, a survey of 100–150 urban, middle-class women aged 18–45 years was conducted in each of eight countries in the developing world. There were striking similarities in perceptions of the pill's health effects between countries: (1) taking the pill is considered to have substantial health risks by 50–75% and is thought to be more hazardous than childbearing by over 40% of respondents except those in the African samples; (2) women who had used the pill are as unaware as those who had not of possible serious cardiovascular adverse effects; (3) the protective effects of the pill are virtually unknown; (4) the greatest inconsistency with scientific evidence concerns the risks of sterility and birth defects attributed to pill use. With information from this survey, family planning programmes can rectify almost universal misperceptions of the pill's safety when counselling new and continuing pill users.

Study on Kidney Function in Female Workers Exposed to Perchlorethylene

1990 ◽  
Vol 9 (6) ◽  
pp. 377-380 ◽  
Author(s):  
A. Vyskočil ◽  
S. Emminger ◽  
J. Tejral ◽  
Z. Fiala ◽  
E. Ettlerova ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Biochemical Parameters ◽  
Renal Damage ◽  
Ambient Air ◽  
Exposed Group ◽  
Control Group ◽  
Total Proteins ◽  
Dry Cleaning ◽  
Female Workers ◽  
Occupationally Exposed

1 Biochemical markers of kidney damage were examined in 16 female workers chronically exposed to tetrachlorethylene (TCE) in five dry-cleaning shops. The results were compared with those obtained in 13 females non-occupationally exposed to organic solvents. 2 The intensity of exposure was monitored by personal environmental monitoring. The time-weighed average exposure to TCE amounted to 157 mg m-3 (range 9-799 mg m-3). A satisfactory agreement was found between the concentration of TCE in ambient air sampled with the charcoal tube method and with a passive dosimeter. 3 The urinary excretion of lysozyme was increased in the exposed group. No difference was found in the urinary excretion of albumin, β2-microglobulin, lactate dehydrogenase, total proteins or glucose. The prevalence of abnormal values of biochemical parameters in the exposed group did not differ from that observed in the control group. No correlation was found between the level of TCE exposure and biochemical parameters. 4 The present study suggests that chronic exposure to TCE does not lead to renal damage.

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