ANALISIS KUALITAS AIR PADA KOLAM PENDEDERAN IKAN LELE DUMBO (Clarias gariepinus) DENGAN PENAMBAHAN TEPUNG DAUN SINGKONG (Manihot utillisima) SEBAGAI PAKAN BUATAN

This study aims to determine the effect of artificial feed by adding cassava leaf flour (Manihot utillisima) to the water quality of the African catfish nursery pond (Clarias gariepinus). This study used the T test (one sample T test) to compare the treatment with artificial feed and commercial feed consisting of 3 replications. The feeding dose was 3% of the weight of the fish for 30 days of maintenance with the frequency of feeding twice a day. Parameters observed were NH3, NO2, NO3, pH, temperature, DO and survival of African catfish. The results showed that the provision of artificial feed in the African catfish nursery did not have a significant effect (P> 0.05) on the observed water quality parameters. The quality of water obtained during maintenance by providing commercial and artificial feed is still supporting the survival of African catfish.

Investigation of Phytoplankton and Physico-chemical Parameters in Nursery, Growout and Broodstock Ponds

The study measures the relationship between physicochemical variables with the cell density of phytoplankton in different stages of pond – nursery ponds, grow out ponds and brood stock ponds. The study was conducted on nine fish ponds as three from each category of pond at Natore Government Fish Farm in Bangladesh, during the months of January to June in 2012. The observed physicochemical variables– water temperature, transparency, dissolved oxygen, pH, ammonia-nitrogen, total alkalinity and total hardness – were found within the standard ranges. Four groups of phytoplankton– Bacillariophyceae, Chlorophyceae, Cyanophyceae and Euglenophyceae– werefound among the ponds where Euglenophyceae was recorded highest number almost in all ponds over the study period. Total abundance of different groups of phytoplankton was recorded as mean (±SD) cell density (cell/l) 62.77±2.16×104, 47.22±0.69×104, and 77.12±3.42×104 in nursery pond, grow out pond and brood stock pond, respectively. Overall phytoplankton was found better in brood stock pond than others. Total phytoplankton density has been exhibited significantly positive correlation with DO and inverse relation with water temperature, pH, ammonia-nitrogen and total alkalinity in case of nursery pond. In case of grow out pond, total phytoplankton density has been exhibited significantly positive correlation with temperature and transparency, and significantly negative correlation with others physicochemical characteristics. In case of brood stock pond, total phytoplankton density has no significant relationship with any physicochemical variables of water.

Kerusakan Dan Jumlah Hemosit Udang Windu (Penaeus monodon Fab.) Yang Mengalami Zoothamniosis

Tiger shrimp (Penaeus monodon Fab.) is one of the biggest non-oil and natural gas export fisheries commodity. In the other hand, the culture of this shrimp is highly affected by infectious pathogens and disease control is ciliated protozoa, Zoothamnium penaei that play an important role as ectocommensals on the body surface of tiger shrimp and causes “udang lumutan” disease. The aim of this research are to know the destruction and mean total haemocyte count of the Tiger shrimp between healthy and easy, middle and heavy infected degree of Zoothamnium penaei. The healthy and was identified infected Zoothamnium penaei of Tiger shrimp samples collected from one of the nursery pond in Lamongan that divided of 50 healthy indivisuals and infected Zoothamnium penaei (50 individuals for each infestation degree). Haemolymph (haemocytes) was obtained from the ventral part of the haemocoel of the second abdominal segment using 1 ml syringe filled with EDTA (Ethylene Diamine Tetra Acetic Acid). The variable observed were destruction of haemocyte and mean total haemocyte count during 7 days. ANOVA (Analysis Of Varian) was used for mean total haemocyte count change data analysis followed by Duncan’s Multiple Range Test with 5% significantly. The research results revealed that Tiger shrimp that easy, middle and heavy infected of Zoothamnium penaei no destruction occurs, but Tiger shrimp that heavy infected of Zoothamnium penaei occurs haemocyte sitoplasma destruction. The highest mean total haemocyte count change was 61,64x106 ± 3,56x106 cells ml-1 in Tiger shrimp that middle infected of Zoothamnium penaei, while the lowest mean total haemocyte count change was 41,04x106 ± 3,64x106 cells ml-1 occur in Tiger shrimp that heavy infected of Zoothamnium penaei.

Dynamics of the bacterial community in a channel catfish nursery pond with a cage–pond integration system

The changes in the bacterial community composition in a channel catfish nursery pond with a cage–pond integration system were investigated by sequencing of the 16S rRNA gene through Illumina MiSeq sequencing platforms. A total of 1 362 877 sequences and 1440 operational taxonomic units were obtained. Further analysis showed that the dominant phyla in the cage and pond groups were similar, including Actinobacteria, Cyanobacteria, Proteobacteria, and Bacteroidetes, although a significant difference was detected between them by ANOSIM (P < 0.05). Temporal changes and site variation were significantly related to the variation of the bacterial community. A comprehensive analysis of the diversity and evenness of the bacterial 16S rRNA gene, redundancy analysis (RDA), and partial Mantel test showed that the bacterial community composition in a cage–pond integration system was shaped more by temporal variation than by site variation. RDA also indicated that water temperature, total dissolved solids, and Secchi depth had the largest impact on bacterial populations.

Effects of stocking density on growth, survival and production of mirror carp (Cyprinus carpio var. specularis) spawn in nursery pond

The research work was conducted to evaluate the effects of stocking density on growth, survival and production of mirror carp (Cyprinus carpio var. specularis) spawn in nursery pond for a period of 35 days. Three treatments differing in stocking density of hatchling viz., T1:172900 individuals/ha, T2:148200 individuals/ha and T3:123500 individuals/ha were employed each having three replicates. Fries were fed same diet in three different treatments consisting of (28.5%) at the rate of 6-10% of body weight. The Physico-chemical characteristics of pond water were measured weekly. The mean values of some water quality parameters such as temperature (°C) were 30.41 ±1.15 (T1), 30.38±1.08 (T2), 30.45±1.10 (T3); transparency (cm) 40.00±0.90 (T1), 39.74±0.51 (T2), 39.93±0.70 (T3); dissolved oxygen (mg/l) 6.79±0.45 (T1), 6.71±0.38 (T2), 7.02±0.29 (T3); pH 7.55±0.18 (T1), 7.67±0.16 (T2), 7.62±0.20 (T3) and alkalinity (mg/l) 116.20±0.64 (T1), 99.36±0.45 (T2), 96.47±0.93 (T3) from 35 days respectively. Except alkalinity no significant value were found for parameters. Sampling was also done weekly. The mean value of final weight (g) was 0.35±0.004 (T1), 0.42±0.008 (T2), 0.59±0.006 (T3). The survival rate of Cyprinus carpio var. specularis was 54.20% (T1), 62.90% (T2) and 74.56% (T3) respectively. The highest survivability was found in T3. The production (kg/ha) of Cyprinus carpio var. specularis was 76.32±4.96 (T1), 77.60±5.19 (T2), 91.04±6.02 (T3) respectively. Significantly (p< 0.05) highest production was found in T3.Asian J. Med. Biol. Res. September 2016, 2(3): 429-435

Investigation of Phytoplankton and Physico-chemical Parameters in Nursery, Growout and Broodstock Ponds

The study measures the relationship between physico-chemical variables with the cell density of phytoplankton in nursery, growout and broodstock ponds of fish. This study was conducted at Natore Government Fish Farm in Bangladesh from January to June, 2012. The observed physico-chemical variables like water temperature, transparency, dissolved oxygen, pH, ammonia-nitrogen, total alkalinity and total hardness were found within the standard ranges. Phytoplankton belonging to bacillariophyceae, chlorophyceae, cyanophyceae and euglenophyceae were found among the ponds but euglenophyceae with highest abundance was recorded in almost all the ponds. Total abundance of different groups of phytoplankton was recorded as mean (±SD) cell density (cell/l) (62.77±2.16)×104, (47.22±0.69)×104, and (77.12±3.42)×104 in nursery pond, growout pond and broodstock pond, respectively. Overall phytoplankton abundance was more in broodstock pond than in others. Total phytoplankton density has exhibited significantly positive correlation with dissolved oxygen (DO) and inverse relation with water temperature, pH, ammonia-nitrogen and total alkalinity in case of nursery pond. For growout pond, total phytoplankton density has exhibited significantly positive correlation with temperature and transparency, and significantly negative correlation with other physico-chemical characteristics. In case of broodstock pond, total phytoplankton density has no significant relationship with any physico-chemical variables of water. Keywords: Nursery pond; Growout pond; Broodstock pond; Phytoplankton; Water quality, physico-chemical parameters © 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v5i3.14886 J. Sci. Res. 5 (3), 555-571 (2013)

Efficacy of two inducing agents PG and DoM+SGnRH in the induced breeding of the major carp, kalibaus (Labeo calbasu)

An experiment on the induced breeding of the endangered fish, Labeo calbasu (Hamilton-Buchanam) was conducted in the Fish Seed Multiplication Farm,Rajshahi to know the efficacy of two inducing agents (PG and DoM+SGnRH). Three breeding trials of each inducing agent were performed. A total of 24 females weighing from 1.5 kg to 2 kg were given an initial and a resolving dose of 1.5 mg and 6 mg PG extract per kg body weight respectively as treatment-1. On the other hand, a total of 24 females weighing from 1.5 kg to 2 kg were given a single dose of 12 mg DoM + SGnRH/kg as treatment-2. In case of treatment-1, 12 males weighing from 1.5 kg to 1.95 kg were administered a single dose of 1.5 mg PG/kg body weight during resolving dose of female. In treatment-2, 12 males weighing from 1.5 kg to 1.8 kg were administered 3 mg DoM+SGnRH /kg body weight during initial dose of females. In treatment-1, the time interval between initial and resolving dose was 5 hours and ovulation occurred in all the injected females within 6 hours after resolving dose. Ovulation occurred within 6 to 8 hours after the injection of inducing agents for treatment-2. The mean rates of ovulation, fertilization and hatching were 100%, 77.36% and 74.5% respectively in treatment-1. On the contrary, the mean rates of ovulation, fertilization and hatching were 83.33%, 63.83% and 59.66% in treatment-2. Hatchery produced fry were reared in nursery pond for 40 days. In nursery pond. Flour, oil cake and wheat bran were applied as nursery feeds. Both the inducing agents were effective in respect of overall breeding performance. But the best results were obtained with PG although in case of DoM+SGnRH complete breeding takes place within short time with less labour and cost than that of PG.DOI: http://dx.doi.org/10.3329/ujzru.v31i0.15376Univ. j. zool. Rajshahi Univ. Vol. 31, 2012 pp. 23-26