scholarly journals Comparative Effects of Industrial Effluents and sub-metropolitan Sewage of Biratnagar on Germination and seedling growth of Rice and Blackgram

Our Nature ◽  
1970 ◽  
Vol 1 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Bhabindra Niroula
Keyword(s):  
Toxic Effect ◽  
Seedling Growth ◽  
Industrial Effluents ◽  
Lethal Effect ◽  
Toxic Effects ◽  
Black Gram ◽  
Leather Industry ◽  
Iron And Steel ◽  
Power House ◽  
Industry Effluent

Comparative effects of effluents from six major industries viz. Diesel Power House, Hetaunda Iron and Steel, Hulas Wire, Himalaya Soap and Chemicals, Leather Industry, Shah Udyog and sub-metropolitan Sewage of Biratnagar on germination and seedling growth of rice and black gram were studied. Effluent of Himalaya Soap and Chemicals showed toxic lethal effect on both the test crops. On germination rice remained more sensitive and susceptible to the toxic effects of industrial effluents but black gram proved to be more tolerant. Effluents of Diesel power House and Shah Udyog remained toxic for seedling growth of black gram as their effects were significant while Leather Industry effluent showed toxic effect on rice for germination as well as seedling growth. Keywords: Industrial effluents, Sewage, Germinationdoi:10.3126/on.v1i1.296Our Nature (2003) 1: 10-14

THE UTILIZATION OF SINGLE ORGANIC NITROGEN COMPOUNDS BY WHEAT SEEDLINGS AND BY PHYTOPHTHORA PARASITICA

1957 ◽  
Vol 35 (4) ◽  
pp. 445-448 ◽  
Author(s):  
William Newton
Keyword(s):  
Growth Inhibition ◽  
Toxic Effect ◽  
Seedling Growth ◽  
Organic Nitrogen ◽  
The Other ◽  
Toxic Effects ◽  
Nitrogen Compounds ◽  
Optical Isomers ◽  
Wheat Seedlings ◽  
Aseptic Conditions

The growth of wheat seedlings under aseptic conditions is shown to be well supported by L-alanine, glycine, and urea, but not so well as by nitrate. On the other hand, D-alanine and the optical isomers of leucine exerted toxic effects; seedling growth in these solutions was less than in the cultures receiving no nitrogen, and the presence of nitrate in the nutrients did not reduce their toxic effects. However, the toxic effect of D-alanine was partially offset by L-alanine. A somewhat parallel influence upon the growth of wheat seedlings and P. parasitica was exerted by D- and L-alanine, D- and L-leucine, and glycine, but not by urea. The latter poorly supported the fungus although it helped the growth of wheat. D-leucine is more toxic to P. parasitica than L-leucine, less toxic to wheat seedlings as measured by growth inhibition, but more toxic as measured by chlorotic lesions in the foliage.

scholarly journals Isolation, Screening, Characterization and Identification of Alkaline Protease Producing Bacteria from Leather Industry Effluent

2021 ◽  
Author(s):  
Chandran Masi ◽  
Getachew Gemechu ◽  
Mesfin Tafesse
Keyword(s):  
Alkaline Protease ◽  
Protease Activity ◽  
Bacterial Species ◽  
Skim Milk ◽  
Industrial Effluents ◽  
Leather Industry ◽  
Skim Milk Agar ◽  
Secondary Screening ◽  
Agar Media ◽  
Industry Effluent

Abstract BackgroundA wide variety of Bacterial species produces protease enzyme and the application of same enzyme have been manipulated precisely and used in various biotechnological areas including industrial and environmental sectors. The main aim of this research study was to isolate, screen and identify protease producing bacteria which were sampled from leather industry effluent present in the outer skirts of Addis Ababa, Ethiopia.PurposeTo isolated alkaline protease producing bacteria from leather industrial effluents and to characterization (Secreening and identification).MethodsSample collected from Modji leather industrial effluents and stored in the microbiology lab. After isolated bacteria from effluent using serial dilution and followed by isolate protease producing bacteria using skim milk agar media. After studying Primary and secondary screening using zonal inhibition methods to select potential protease producing bacteria using skim milk agar media. Finally to characterization and identification of potential bacteria using biochemical methods, protein estimation, biomass, protease assay and gene sequencing (16S rRNA) method to finalized best protease producing bacteria. ResultsTwenty-eight different bacterial colonies were isolated initially from the leather industry effluent sample situated at Modjo town of Ethiopia. The isolated bacteria were screened using primary screening method with skim milk agar medium. Three isolates namely MS12, ML5 and ML12 showing highest zone of proteolysis as a result of casein degradation on the agar plates were selected and subjected to secondary screening. Further secondary secreening confirmed that MS12, ML5 and ML12 has efficient proteolytic activity and can be considered as potent protease producer. The three isolates were then subjected to morphological and biochemical tests to identify probably bacterial species and all the three bacterial isolates were found out to be of Bacillus species. Shake flask method was carried out to identify the most potent one having greater biomass production capabilities, protein quantity and protease activity. ML12 isolated from leather effluent waste showed highest Protein(170mg/ml), Protease activity(19U/ml), high biomass production and the same was subjected to molecular identification using 16s sequencing and a Phylogenetic tree was constructed to identify the closest neighbor. The isolate ML12 is 97.87% homologous to Bacillus cereus strain (KY995152.1) and 97.86% homologous to Bacillus cereus strain (MK968813.1).ConclusionsThis study has revealed that the leather industry effluent site has significant feature of housing potent bacterial species producing protease of commercial value. Being one among the most widely used enzyme, comparatively. Protease holds a larger scope for research and commercialization any other type of enzymes. There is a need to develop novel protease enzymes for further necessary applications of these enzymes. Moreover, enzyme produced by bacteria which are present in effluents are a greater boon to establish the significance of converting industrial wastes to a highly valuable enzymes especially like proteases.

scholarly journals Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Chandran Masi ◽  
Getachew Gemechu ◽  
Mesfin Tafesse
Keyword(s):  
Bacillus Cereus ◽  
Alkaline Protease ◽  
Protease Activity ◽  
Bacterial Species ◽  
Skim Milk ◽  
Industrial Effluents ◽  
Leather Industry ◽  
Skim Milk Agar ◽  
Secondary Screening ◽  
Industry Effluent

Abstract Background A wide variety of bacterial species produces protease enzyme, and the application of the same enzyme has been manipulated precisely and used in various biotechnological areas including industrial and environmental sectors. The main aim of this research study was to isolate, screen, and identify alkaline protease-producing bacteria that were sampled from leather industry effluent present in the outer skirts of Addis Ababa, Ethiopia. Purpose To isolate and characterize the alkaline protease-producing bacteria from leather industrial effluents. Methods Samples are collected from Modji leather industrial effluents and stored in the microbiology lab. After isolated bacteria from effluent using serial dilution and followed by isolated protease-producing bacteria using skim milk agar media. After studying primary and secondary screening using zonal inhibition methods to select potential protease-producing bacteria using skim milk agar media. Finally, to identify the potential bacteria using biochemical methods, bacterial biomass, protease activity, and gene sequencing (16S rRNA) method to finalize the best alkaline protease producing bacteria identified. Results First twenty-eight different bacterial colonies were isolated initially from the leather industry effluent sample situated at the Modjo town of Ethiopia. The isolated bacteria were screened using the primary and secondary screening method with skim milk agar medium. At the primary level, we selected three isolates namely ML5(14 mm), ML12(18 mm), and MS12 (15 mm), showing the highest zone of proteolysis as a result of casein degradation on the agar plates were selected and subjected to primary screening. Further secondary screening confirmed that the zone of inhibition methods ML5 (14.00±0.75 mm), ML12 (19.50±0.66 mm), and MS12 (15.00±1.32 mm) has efficient proteolytic activity and can be considered as effective protease producer. The three isolates were then subjected to morphological and biochemical tests to identify probably bacterial species, and all the three bacterial isolates were found out to be of Bacillus species. The shake flask method was carried out to identify the most potent one having greater biomass production capabilities and protease activity. ML12 isolated from leather effluent waste showed the highest protease activity (19 U/ml), high biomass production, and the same was subjected to molecular identification using 16s sequencing and a phylogenetic tree was constructed to identify the closest neighbor. The isolate ML12 (Bacillus cereus strain -MN629232.1) is 97.87% homologous to Bacillus cereus strain (KY995152.1) and 97.86% homologous to Bacillus cereus strain (MK968813.1). Conclusions This study has exposed that from twenty-eight different bacterial samples isolated from leather industry effluent; further primary and secondary screening methods were selected three potential alkaline protease strains. Finally, based on its biochemical identification, biomass, and protease activity, ML12 (Bacillus cereus strains) is the best strain identified. The alkaline protease has the significant feature of housing potent bacterial species for producing protease of commercial value.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

Sewage water irrigation effects on some potentially toxic trace elements in soil and potato plants in northwestern India

2000 ◽  
Vol 80 (3) ◽  
pp. 465-471 ◽  
Author(s):  
M. S. Brar ◽  
S. S. Malhi ◽  
A. P. Singh ◽  
C. L. Arora ◽  
K. S. Gill
Keyword(s):  
Solanum Tuberosum ◽  
Ground Water ◽  
Toxic Elements ◽  
Crop Yields ◽  
Solanum Tuberosum L ◽  
Sewage Water ◽  
Potentially Toxic Elements ◽  
Leather Industry ◽  
Northwestern India ◽  
Industry Effluent

Sewage water is used for irrigation to improve crop yields, but it may affect the soil and crop quality. The effects of discharging the effluents of a leather complex on the concentrations of some potentially toxic elements (Cu, Fe, Mn, Zn, Al, As, Cr and Ni) in sewage water and the effects of irrigation with contaminated sewage water on the concentrations of these elements in soils and in potato (Solanum tuberosum L.) leaves and tubers were studied near Jalandhar city, Punjab, in northwestern India. Two treatments were 10 yr of irrigation with 300 mm of sewage water + 300 mm of ground water per annum (SW) and with 600 mm of ground water per annum (GW). Soils of the study fields were Typic Ustochripts with sandy to loamy sand texture. The concentrations of all elements except As increased following the addition of leather complex effluents in the sewage water, with the biggest increase in the concentration of Cr (from 2.7 mg to 14.0 mg Cr L−1). The SW treatment increased concentrations of all elements in soil except As, and the increase was significant to 60 cm depth for Fe, Mn, Zn, Al and Ni and to 30 cm depth for Cu and Cr. Irrigation with SW also increased the concentrations of these elements in potato leaves and tubers and the increase was generally higher in leaves than in tubers. The proportional increase of Cu, Fe, Zn and Al was less in plants than in soils, that of Mn and Cr was almost similar in plants and in soil, and that of Ni was more in plants than in soil. These elements, which accumulate in soils and crops, may become health hazards to humans and/or animals. Therefore, continued monitoring of the concentrations of potentially toxic elements in soil and plants and/or treatment of sewage water before using for irrigation is needed. Key words: Irrigation, leather industry effluent, Solanum tuberosum, potentially toxic elements, sewage water

Toxic Effects of Amino-Acids and Amines on Seedling Growth

Nature ◽  
1947 ◽  
Vol 160 (4059) ◽  
pp. 222-223 ◽  
Author(s):  
L. J. AUDUS ◽  
J. H. QUASTEL
Keyword(s):  
Amino Acids ◽  
Seedling Growth ◽  
Toxic Effects

scholarly journals Comparison between freeze and spray drying to obtain powder Rubrivivax gelatinosus biomass

2013 ◽  
Vol 33 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Edson Francisco do Espírito Santo ◽  
Leandro Kanamaru Franco de Lima ◽  
Ane Pamela Capucci Torres ◽  
Gabriela de Oliveira ◽  
Elisa Helena Giglio Ponsano
Keyword(s):  
Spray Drying ◽  
Freeze Drying ◽  
Bacterial Biomass ◽  
Quality Criterion ◽  
Industrial Effluents ◽  
Product Recovery ◽  
Animal Origin ◽  
Freeze Dried ◽  
Rubrivivax Gelatinosus ◽  
Industry Effluent

The use of colorants in products of animal origin is justified by the improvement in the color of foods since this attribute is considered a quality criterion. These additives can be produced using industrial effluents as substrates and appropriate organisms, such as Rubrivivax gelatinosus. Oxycarotenoids represent a class of carotenes responsible for the pigmentation of animals and vegetables. R. gelatinosus grows in fish industry effluent with the resulting production of a bacterial biomass containing oxycarotenoids. The purpose of this study was to compare the use of two drying processes - spray and freeze drying - to obtain powder biomass in terms of the process parameters (yield, productivity, and product recovery) and the product characteristics (color, proximate composition, and oxycarotenoids). No difference was detected in the yield between these techniques, while productivity was higher using spray drying. Higher product recovery and moisture were achieved with freeze drying, while ash was higher with spray drying. The freeze dried biomass was redder, darker and less saturated than the spray dried biomass. No difference in oxycarotenoids was detected between the biomasses. Although it results in lower recovery rate, spray drying was faster and more productive, and it provided the same yield as freeze drying, which makes it the method of choice for obtaining R. gelatinosus biomass.

scholarly journals ALLELOPATHIC EFFECT OF A WEED SPECIES, CYPERUS ROTUNDUS L. AND CLEOME VISCOSA L. ON GROWTH AND DEVELOPMENT OF BLACK GRAM (VIGNA MUNGO (L.) HEPPER.)

2015 ◽  
Vol 2 (2) ◽  
pp. 123-128
Author(s):  
Prabhakaran J ◽  
Kavitha D ◽  
Arumugam K
Keyword(s):  
Seedling Growth ◽  
Vigna Mungo ◽  
Cyperus Rotundus ◽  
Allelopathic Effect ◽  
Aqueous Extracts ◽  
Black Gram ◽  
Weed Species ◽  
Weight Content ◽  
Whole Plants ◽  
Cleome Viscosa

The present study was aimed to investigate the allelopathic influence of two weed species Cyperus rotundus L. and Cleome viscosa L. against the growth of Black gram (Vigna mungo (L.) Hepper). Aqueous extracts (5%, 10%, 25%, 50%, 75% and 100% concentrations) of whole plants of C. rotundus and C. viscosa were employed to investigate their influence on the germination and seedling growth of the black gram. The aqueous whole plants extracts ( from 5% to 100% concentrations) of C. rotundus and C. viscosa exhibited an inhibitory effects on all the parameters employed in the germination percentage, root and shoot growth, fresh and dry weight, content of chlorophyll, starch, sugar and protein contents of Black gram over control. The extracts of both the weeds caused a minimum inhibition on germination and seedling growth of Black gram at 5% concentration and the intensity of inhibition increased when increased extract concentrations. The aqueous extracts of C. rotundus and C. viscosa showed an inhibitory effect on Black gram but their effect was more severe on black gram by the extract treatments of C. rotundus than that of C. viscosa.

scholarly journals Growth and leaf epidermal response of three Sesamum indicum varieties to industrial effluent irrigation

2017 ◽  
Vol 52 (1) ◽  
pp. 1-6 ◽  
Author(s):  
AA Abdul Rahaman ◽  
OM Olaniran ◽  
FA Oladele
Keyword(s):  
Seedling Growth ◽  
Stomatal Density ◽  
Industrial Effluent ◽  
Industrial Effluents ◽  
Sesamum Indicum ◽  
Proper Treatment ◽  
Trichome Density ◽  
Stomatal Index ◽  
Stomatal Size ◽  
Epidermal Features

The effect of industrial effluents was studied with respect to growth and leaf anatomy of three Sesamum indicum varieties (NGB 00931, NGB 00937 and NGB 00939). Industrial effluents (25%, 50%, 75% and 100%) from two industries are used to irrigate the plants. Although, the control plants possessed larger leaves and longer stems than the effluent-treated plants, at lower concentration, the plant growth is relatively higher. Gradual decrease in the germination of seeds and seedling growth with increase in effluent concentration was observed. The best germination and seedling growth was observed at the 25% concentration. Leaf epidermal features (stomatal density, stomatal index, stomatal size, trichome density, tricome index, trichome size and number of epidermal cells) are more influenced in the effluent-treated plants than in the control plants from the Peace Standard Pharmaceutical Industry than in the effluent from the Global Soap & Detergent Industry. Thus the industrial effluents can be safely used for irrigation purposes with proper treatment and dilution at 25%.Bangladesh J. Sci. Ind. Res. 52(1), 1-6, 2017

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