scholarly journals Comparison of Biofilm Producing and Non-Producing Escherichia coli Isolated from Urine Samples of Patients Visiting a Tertiary Care Hospital of Morang, Nepal

2019 ◽  
Vol 6 ◽  
pp. 76-81
Author(s):  
Manita Tumbahangphe ◽  
Bijay Kumar Shrestha ◽  
Jenish Shakya ◽  
Hemanta Khanal
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Urine Samples ◽  
Diffusion Method ◽  
Care Hospital ◽  
Microtitre Plate ◽  
Plate Method ◽  
E Coli

Objectives: The main objective of this study was to determine the prevalence of Escherichia coli among urinary tract infection (UTI) suspected patients visiting tertiary care hospital and to assess the biofilm producing ability of E. coli isolates. Methods: A prospective cross-sectional study was carried out in Biratnagar Metropolitan city, Eastern Nepal from December 2018 to May 2019. During the study 400 urine samples were collected from UTI suspected patients visiting a tertiary care hospital of Biratnagar. Urine samples were cultured by using semi-quantitative culture technique and identified. Antibiotic susceptibility testing was done by Kirby-Bauer Disk Diffusion method according to CLSI (2011) guidelines. Biofilm assays were performed by microtitre plate method. Results: This study reported 15% prevalence of E. coli out of 400 urine samples. 100% of E. coli isolates showed resistance to both Ampicillin and Amoxicillin while 100% were sensitive to Chloramphenicol. 70% (42/60) isolates were Multi Drug Resistance (MDR)E. coli. The maximum isolates (86.66%) were found to be biofilm producers by microtitre plate method. Resistance to other antibiotics such as Nalidixic acid (71.11% vs 46.66%), Norfloxacin (53.33% vs 46.66%), Cotrimoxazole (42.22% vs 26.66%) was comparatively higher among biofilm producers than non-biofilm producers. There was a significance of association between biofilm and MDR (p<0.05). Conclusion: There is relation between the ability of biofilm formation and drug resistance in the bacterium resulting to the failure of antibacterial drugs.

scholarly journals Antibacterial effect of green tea extract against multi drug resistant Escherichia coli isolated from urine sample of patients visiting tertiary care hospital of Eastern Nepal

2020 ◽  
Vol 8 (1) ◽  
pp. 45-51
Author(s):  
Kabita Giri ◽  
Bijay Kumar Shrestha ◽  
Jenish Shakya ◽  
Shiv Nandan Sah ◽  
Hemanta Khanal
Keyword(s):  
Escherichia Coli ◽  
Green Tea ◽  
Antibacterial Effect ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Green Tea Extract ◽  
Care Hospital ◽  
Drug Resistant ◽  
E Coli ◽  
Tea Extract

Urinary tract infections (UTIs) caused by drug resistant (DR) Uropathogenic Escherichia coli have become a significant worldwide public health problem. Green tea (Camellia sinensis), has been reported to have antimicrobial activities against various pathogenic bacteria. The main aim of our study was to estimate the antibacterial effect of green tea extract against drug resistant Uropathogenic E. coli isolated from urine samples of patients visiting in tertiary care hospital from eastern Nepal. During the study 360 urine samples were collected from UTI suspected patients visiting a tertiary care hospital of Biratnagar. Urine samples were cultured by using semi-quantitative culture technique and bacteria was identified by standard microbiological procedure. Antibiotic susceptibility testing was done by Kirby-Bauer Disk Diffusion method according to NCCLS (2011) guidelines. The antibacterial effect of green tea extract was performed by preparing the 95% ethanol extract in Soxhlet apparatus which was dispensed in DMSO solution and sterilized by membrane filtration. Antibacterial activity of Green Tea Extract against MDR Uropathogenic Escherichia coli was performed by making different concentration of green tea. The overall prevalence of E. coli was 27.22% in study population whereas the prevalence of MDR E. coli was 21.08%. All the isolated E. coli exhibited 100% sensitivity towards Nitrofurantoin and it was still a drug of choice for the treatment of Urinary tract infection caused by E. coli. The green tea extracts exhibited effective antibacterial activity against MDR E. coli. The MIC of Green Tea Extract was found to be 600µg/ml for 24 MDR isolates and 1000µg/ml for remaining 11 isolates. Based on the present study it is concluded that Green Tea extracts have great potential as an antimicrobial agent against E. coli. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 45-51

scholarly journals Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal

2016 ◽  
Vol 5 (2) ◽  
pp. 51-55 ◽  
Author(s):  
Bigu Kumar Chaudhari ◽  
Ganesh Kumar Singh ◽  
Kamal Prasad Parajuli ◽  
Kewal Shrestha
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Diffusion Method ◽  
Care Hospital ◽  
E Coli ◽  
Medical College ◽  
Tract Infections

Background Urinary Tract Infection (UTI) is one of the most common infectious diseases which affect almost all ages groups of population. Production of â-lactamases is responsible for antibacterial resistance which is frequently observed in Enterobacteriaceae isolates, particularly by E. coli and Klebsiella pneumoniae. This investigation has been carried out to determine the current status of prevalence and susceptibility of uropathogens isolated among the patients at tertiary care hospital in eastern Nepal.Material and Methods This study was done at the department of Microbiology, Nobel Medical College Teaching Hospital, Biratnagar, Nepal during May 1st 2015 to October 31st 2015. Midstream cleancatch urine was sampled from 1730 suspected urinary tract infection patients of different age and sex groups. Uropathogens were recognized in term of standard and specific microbiological techniques and antimicrobial susceptibility pattern was determined by Kirby Bauer Disc diffusion method following Clinical and Laboratory Standards Institute (CLSI) guidelines.Results Out of 1730 suspected specimens Culture resulted a total of 761 (43.98 %) positive and 969 (56.02%) negative among that significant growths of uropathogens including 700 (91.98 %) unimicrobial and 60 (7.88 %) polymicrobial growths. In term of Gender distribution 443 (25.60 %) were male and 1287 (74.40 %) were female hence the ratio is 0.34:1, respectively. E. coli was the leading isolate (66 %), followed by Klebsiella spp. (12 %), Enterococcus spp. (8 %), Pseudomonas spp. (6 %), Acinetobacter anitratus (5 %), Proteus spp. (3 %).Conclusion The high frequency of multidrug resistance in bacterial uropathogens was seen. Principally, resistance patterns were seen higher for amoxycillin, co-trimoxazole, flouroquinolones and third-generation cephalosporins, Existing uropathogens highlights the highest rate of vulnerability to nitrofurantoin, amikacin and gentamicin which provide much better antibiotic coverage and can be adapted for practical treatment of urinary tract infections.  Journal of Nobel Medical College Vol.5(2) 2016; 51-55

scholarly journals ESBL-producing Escherichia coli and Klebsiella pneumoniae at a tertiary care hospital in Saudi Arabia

2014 ◽  
Vol 8 (09) ◽  
pp. 1129-1136 ◽  
Author(s):  
Ali Mohammed Somily ◽  
Hanan A Habib ◽  
Muhammad M Absar ◽  
Muhammad Z Arshad ◽  
Kutubu Manneh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clavulanic Acid ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
University Hospital ◽  
Care Hospital ◽  
Microbiology Laboratory ◽  
Blood Samples ◽  
E Coli ◽  
Sterile Body Fluids

Introduction: The increasing frequency  and antibiotic resistance among extended-spectrum β-lactamases (ESBLs)-producing bacteria are posing a serious threat. This study sought to investigate the frequency and antibiotic susceptibility of ESBL-producing E. coli and K. pneumoniae at a tertiary care hospital. Methodology: Data were collected from samples sent to the microbiology laboratory between 2006 and 2010 at King Khalid University Hospital, Riyadh. ESBLs were confirmed using Etest strips of cefotaxime/cefotaxime + clavulanic acid, ceftazidime/ceftazidime + clavulanic acid, and cefepime/cefepime + clavulanate. Results: Out of 17,105 samples, 1,076 (6.3%) ESBL-producing isolates of E. coli (808) and K. pneumoniae (268) were confirmed. Among these, 680 (63.2%) isolates were found in urine samples, followed by 287 (26.7%) in superficial swabs, deep wounds swabs, tissues and sterile body fluids, 71 (6.6%) in respiratory, and 38 (3.5%) in blood samples. The overall frequency rates of ESBL E. coli and K. pneumoniae were 6.6% and 5.5%, respectively. The frequency of ESBL-producing E. coli and K. pneumoniae increased significantly during the study period. E. coli resistance against cotrimoxazole was 71.1%, followed by ciprofloxacin (68.2%) and gentamicin (47%). Similarly, 62.7% of K. pneumoniae isolates were resistant to gentamicin, 59.5% to cotrimoxazole, and 49.8% to ciprofloxacin. There was no statistically significant change in antimicrobial resistance over the study period. Conclusions: Although the frequency rates of ESBL-producing E. coli and K. pneumoniae increased, no change in the anti-microbial susceptibility was observed over the study period.

scholarly journals Molecular characterization and antimicrobial susceptibility profile of New Delhi metallo-beta-lactamase-1-producing Escherichia coli among hospitalized patients

2018 ◽  
Vol 10 (02) ◽  
pp. 149-154
Author(s):  
Anjali Agarwal ◽  
Jyoti Srivastava ◽  
Ujjwal Maheshwari ◽  
Mohd Iftikhar
Keyword(s):  
Escherichia Coli ◽  
Tertiary Care Hospital ◽  
Treatment Options ◽  
Tertiary Care ◽  
Hospitalized Patients ◽  
New Delhi ◽  
Care Hospital ◽  
Beta Lactamase ◽  
Cross Sectional ◽  
E Coli

ABSTRACT BACKGROUND: Carbapenemase-producing microorganisms are becoming a major concern among hospital-acquired infections. There is also increased multidrug resistance seen among these isolates. AIMS: We have conducted this study to determine the prevalence of New Delhi metallo-beta-lactamase-1 (NDM-1) gene-producing Escherichia coli among hospitalized patients in a tertiary care hospital in Northern India. SETTINGS AND DESIGN: The study was conducted in the Department of Microbiology with the tertiary care hospital settings. It was a prospective cross-sectional observational study conducted during January 2014–August 2014. MATERIALS AND METHODS: A total of 500 nonduplicate E. coli samples were processed. The isolates with reduced susceptibility to ertapenem, i.e., zone diameter between 19 and 21 mm, were considered carbapenemase producers. These isolates were subjected to modified Hodge test for phenotypic confirmation. Polymerase chain reaction was performed on all the screened isolates for molecular detection of NDM-1 gene. STATISTICAL ANALYSIS USED: Chi-square test was used to analyze the data and P < 0.05 was considered statistically significant. RESULTS: Out of 500 E. coli isolates, 61 (12.2%) were screened for carbapenemase production. 47 (9.4%) isolates were positive by modified Hodge test and 36 (7.2%) isolates showed the presence of blaNDM-1 gene (P < 0.05). CONCLUSION: There is an increased prevalence of NDM-1 gene-producing E. coli isolates. These carbapenemase-producing isolates are more resistant to other group of antibiotics (aminoglycosides, fluoroquinolones along with β-lactam group). Early detection of blaNDM-1 gene can help in choosing the effective treatment options for hospitalized patients in time, thereby reducing the risk of mortality.

scholarly journals Nosocomial blood-stream infections from extended-spectrum-beta-lactamase-producing Escherichia coli and Klebsiella pneumonia from GB Pant Hospital, New Delhi

2010 ◽  
Vol 4 (08) ◽  
pp. 517-520 ◽  
Author(s):  
Juhi Taneja ◽  
Bibhabati Mishra ◽  
Archana Thakur ◽  
Vinita Dogra ◽  
Poonam Loomba
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Test Method ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Care Hospital ◽  
E Coli ◽  
Extended Spectrum ◽  
Esbl Producers

Background: Nosocomial septicemia due to extended spectrum beta-(β)-lactamase (ESBL) producing Klebsiella pneumoniae and Escherichia coli are a therapeutic challenge due to resistance. Knowledge of disease burden and resistance patterns is required for proper and timely management. We report the prevalence and antimicrobial susceptibility of ESBL producing E. coli and K .pneumoniae from septicemia at a tertiary care hospital. Methodology: A total of 2,870 blood samples of suspected cases of septicemia were studied between January and December 2009. Antimicrobial susceptibility was determined by Kirby Bauer's disc diffusion method and MICs for imipenem, meropenem, and ertapenem were determined using the E-test. All isolates of E. coli and K. pneumoniae were tested for ESBL production by E-test method. Results: Forty-one (70.7%) K. pneumoniae isolates and ten (41.7%)  E. coli isolates were ESBL producers. Two (5%) of ESBL producing K. pneumoniae isolates, but no E. coli isolates, were resistant to carbapenems. In vitro, all ESBL producers were sensitive to tigecycline. Conclusion: Our data indicated that the prevalence of ESBL-producing E. coli and K. pneumonia strains isolated from blood cultures from hospitalized patients is high. ESBL-producing organisms were found to be more susceptible to meropenem than to imipenem and ertapenem. Tigecycline is active against all the ESBL or multidrug resistant (MDR) E. coli and Klebsiella spp. isolates.

Emergence of Third generation Cephalosporin Resistant Escherichia coli in infants: Retrospective Study from a Tertiary Care Hospital in South India

2020 ◽  
Vol 18 ◽  
Author(s):  
Santhosh J. Thattil ◽  
Thekkuttuparambil A. Ajith
Keyword(s):  
Retrospective Study ◽  
Tertiary Care Hospital ◽  
Throat Swab ◽  
Tertiary Care ◽  
Generation Cephalosporin ◽  
Urine Samples ◽  
Care Hospital ◽  
Third Generation ◽  
E Coli ◽  
Sensitivity Pattern

Aim: This study was aimed to evaluate the emergence of third generation cephalosporin resistant Escherichiacoli isolated from the culture of various biological fluids of infants in a tertiary care hospital. Background: :Resistance to commonly used antibiotics is consistently increasing during the last decades and it was found varying with time and geographical location. Objectives: To determine the prevalence of third generation cephalosporin sensitivity pattern of E. coli isolated from urine, stool, and throat swab culture from laboratory records. Methods: Retrospective study included the laboratory report of antibiotic sensitivity conducted on infants specimens during the period of 2 years. Third generation cephalosporin sensitivity pattern of E. coli isolated from urine, stool, and throat swab culture were collected and data were statistically analyzed. Results: Reports of 351 samples with positive E. coli were included in the study. More samples were analyzed from infants above 6 months of age with female dominance (184/351). Among the specimens tested, urine samples were highest 128/351 (35.0%). Cephalosporin resistant strain was found in 243/351 samples (69.2%) (p=0.0463) with 218/243 (89.7%) isolated from infants above 6 months of age and 25/243 (10.2%) were from infants below 6 months of age (p=0.038). The frequency of cephalosporin resistant E.coli in urine specimens of male and female infants was significant (p=0.0001). Conclusion: Third generation cephalosporin resistant E. coli strain was found in 69.2% of isolates and more in urine samples from female infants above 6 months of age. The clinicians should consider the emergence of cephalosporin resistantE. coli while selecting antibiotics as the empirical treatment.

Composition of Escherichia coli population in the neonatal gut: phylogroups and virulence determinants

2013 ◽  
Vol 62 (11) ◽  
pp. 1680-1687 ◽  
Author(s):  
Parijat Das ◽  
Arun Kumarendu Singh ◽  
Suchandra Mukherjee ◽  
Krishnan Rajendran ◽  
Dhira Rani Saha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Neonatal Intensive Care ◽  
Tertiary Care Hospital ◽  
Multinomial Logistic Regression ◽  
Tertiary Care ◽  
Care Hospital ◽  
Virulence Determinants ◽  
Phylogenetic Groups ◽  
E Coli ◽  
Insight Into

The composition of Escherichia coli in the neonatal gut has rarely been studied in developing countries. To gain insight into the composition of E. coli in the neonatal gut and to assess factors that could influence colonization by E. coli, analysis of the phylogenetic groups and virulence determinants of E. coli isolated from the guts of neonates in a tertiary care hospital was carried out. The distribution of the phylogroups of 124 E. coli isolates recovered showed that phylogroups A (23 %) and B1 (49 %) accounted for 72 % of the isolates. Isolates of the phylogenetic group B2 were rare (8 %). Virulence factors were also rare with the exception of aerobactin (iucC), which was detected in 45 % of the isolates and was significantly associated with phylogroup B1. Multinomial logistic regression established that colonization with phylogroup B1 was associated with a stay in the neonatal intensive care unit; phylogroup A was associated with a stay on the ward; and phylogroups B2 and D were associated with neonates delivered vaginally. Evaluation of the effect of different E. coli phylogroups, with and without identified virulence determinants, on the gut of neonatal mice showed histopathological changes in the mucosa. The severity of the changes could be correlated with the presence of virulence determinants, irrespective of the phylogroup.

Sign in / Sign up

Export Citation Format

Share Document