scholarly journals Morphological and morphometric analysis of tissue changes during implantation of polypropylene mesh implants modified with carbon nanotubes and antiseptics

2018 ◽  
Vol 22 (1) ◽  
pp. 19-23
Author(s):  
R.A. Lutkovsky
Keyword(s):  
Carbon Nanotubes ◽  
Connective Tissue ◽  
Polypropylene Mesh ◽  
Antimicrobial Properties ◽  
Tissue Response ◽  
Digital Data ◽  
Connective Tissue Capsule ◽  
Series Of Experiments ◽  
Tissue Reactions ◽  
Tissue Capsule

Despite the introduction of modern materials for the plasticity of tissues in hernia, the results of treatment of this pathology are not entirely satisfied with the surgeons. A large number of complications and hernias recurrences, encourages the search for materials that would not cause tissue reactions to the foreign body and would have the proper antimicrobial properties. We have developed new mesh implants from modified polypropylene with carbon nanotubes and an antiseptic. The aim of the study is to conduct a comparative analysis of the tissue response to the implantation of mesh implants from polypropylene modified with carbon nanotubes and the antiseptic polyhexamethylene guanidine chloride. During the research, international norms and laws of Ukraine on bioethics adhered to. The study was performed on 70 laboratory rats in two series of experiments on 35 animals in each. In the first series, a polypropylene mesh was implanted, and in the second — a developed mesh. The animals were withdrawn from the experiment 3, 7, 14, 21, 30 and 90 days from the start of the experiment, and the material was taken for study. The selected abdominal wall tissues along with the mesh implants were fixed, poured into paraffin and slides were prepared, stained with hematoxylin-eosin and van Gieson, after what was examined under a microscope. The obtained digital data of the studies, in both series of experiments, were compared and subjected to statistical processing. The conducted studies made it possible to establish that caused inflammation by the implantation of the developed nanocomposite implants in the tissues disappear more rapidly than due to classical implants from polypropylene. At the same time, the formation of a connective tissue capsule that dissociated the implants from the tissues was completed before the 14th day of the experiment. At that time around the classical polypropylene implants during this observation period in the tissues minor inflammatory phenomena were observed, and the formation of the connective tissue capsule was completed until to 30 days of observation. So, high biocompatibility of the developed implants with tissues, what creates the prospect of their application in the clinic for the plastic of tissues was experimentally established.

scholarly journals A Quiescent Colossal Radicular Cyst: A Diverse Histological Presentation

2013 ◽  
Vol 4 (4) ◽  
pp. 286-290
Author(s):  
V Reshma ◽  
Radhika M Bavle ◽  
BK Varsha ◽  
BM Kavya
Keyword(s):  
Connective Tissue ◽  
Periodontal Ligament ◽  
Radicular Cyst ◽  
Cell Infiltrate ◽  
Jaw Cysts ◽  
Connective Tissue Capsule ◽  
Tissue Capsule

ABSTRACT Radicular cysts are the most frequent inflammatory cysts comprising of about 52 to 68% of all the jaw cysts. It arises from the epithelial residues in the periodontal ligament space following pulpal necrosis. The distinguished lining of the cystic lumen is hyperplastic stratified squamous nonkeratinizing epithelium showing arcading pattern with dense infammatory cell infiltrate, cholesterol clefts, Russell bodies and Ruston bodies in the connective tissue capsule. Here, we present an unusual large radicular cyst lined almost entirely by quiescent atrophic epithelium, which is innocuous, persistent, has enlarged overtime, with minimal infammatory cells in the connective tissue capsule. How to cite this article Reshma V, Bavle RM, Varsha BK, Kavya BM. A Quiescent Colossal Radicular Cyst: A Diver Histological Presentation. World J Dent 2013;4(4):286-290.

scholarly journals Implants and bone technology with the use of doped calcium carbonate phosphates

2020 ◽  
pp. 54-61
Author(s):  
L. F. Koroleva ◽  
◽  
L. P. Larionov ◽  
M. N. Dobrinskaya ◽  
◽  
...  
Keyword(s):  
Drug Delivery ◽  
Calcium Carbonate ◽  
Connective Tissue ◽  
Blood Vessels ◽  
Bone Repair ◽  
Living Organism ◽  
Nerve Endings ◽  
Histological Studies ◽  
Connective Tissue Capsule ◽  
Tissue Capsule

The possibility of obtaining an alloplastic biomaterial for the implant based on doped calcium carbonate-phosphates and polycaprolactone is studied. Nanocrystalline calcium carbonate-phosphate doped with cations of iron, magnesium, potassium, zinc, manganese, and silicon intended for bone repair by drug delivery is investigated. Histological studies have revealed that samples after 60 days in a living organism are covered by a connective tissue capsule. The formation of blood vessels and nerve endings is observed in the capsule.

scholarly journals SOME TOPOGRAPHICAL AND HISTOLOGICAL STUDIES OF THYROID AND PARATHYROD GLANDS OF MOORHEN (Gallinula c. chloropus)

2021 ◽  
Vol 22 (1) ◽  
pp. 85-96
Author(s):  
K.K. Kadhim ◽  
N.S. Al-Samarrae ◽  
J.Y. Al-Fayas
Keyword(s):  
Thyroid Gland ◽  
Connective Tissue ◽  
Parathyroid Glands ◽  
Histological Studies ◽  
Connective Tissue Capsule ◽  
Small Branch ◽  
Thyroid Glands ◽  
The Common ◽  
Parenchymal Cells ◽  
Tissue Capsule

 The thyroid gland of Moorhen has two separated lobes. These lobes were located in the throracic inlet and receive blood supply from the cranial, middle and caudal thyroid arteries. The histological organization of the thyroid gland in Moorhen is surrounded by a distinct connective tissue capsule and the parenchymal cells were arranged into colloid filled follicles enmeshed in the highly vascular interstitial connective tissue. The bilaterally paired, round to oval, parathyroid glands in Moorhen were located intrathoracically near or close to the caudal pole of the thyroid glands. They receive blood by short branches from caudal thyroid artery and small branch from the common carotid artery. The parathyroid glands in Moorhen have a thin connective tissue capsule. Its parenchymal cells were arranged into an irregular, anastomosing cords of chief cells. No oxyphil cells were found in the parathyroid glands of Moorhen.

Alteration of deposition pattern and pulmonary response as a result of improved dispersion of aspirated single-walled carbon nanotubes in a mouse model

2008 ◽  
Vol 294 (1) ◽  
pp. L87-L97 ◽  
Author(s):  
R. R. Mercer ◽  
J. Scabilloni ◽  
L. Wang ◽  
E. Kisin ◽  
A. R. Murray ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Connective Tissue ◽  
Single Walled Carbon Nanotubes ◽  
Tissue Response ◽  
Mouse Lung ◽  
Single Walled Carbon ◽  
Inflammatory Phase ◽  
Macrophage Phagocytosis ◽  
Granulomatous Lesions ◽  
Walled Carbon Nanotubes

Nanoparticles have a fundamental dimension of <100 nm. However, on suspension in media, agglomerates of nanoparticles are the more common structure. This is particularly evident in prior intratracheal instillation or aspiration studies of single-walled carbon nanotubes (SWCNT), in which granulomatous lesions encased by epithelioid macrophages were produced by large agglomerates. In this study, we tested the hypothesis of whether exposure to more dispersed SWCNT structures would alter pulmonary distribution and response. A dispersed preparation of single-walled carbon nanotubes (DSWCNT) with a mean diameter of 0.69 μm was given by pharyngeal aspiration to C57BL/6 mice. Electron microscopy demonstrated a highly dispersed, interstitial distribution of DSWCNT deposits by 1 day postexposure. Deposits were generally <1 μm. Macrophage phagocytosis of DSWCNT was rarely observed at any time point. Lung responses were studied by lavage and morphometry at 1 h, 1 day, 7 day, and 1 mo after a single DSWCNT exposure of 10 μg/mouse. Lung sections and lavage cells demonstrated an early, transient neutrophilic and inflammatory phase that rapidly resolved and was similar to that observed with large agglomerates. No granulomatous lesions or epithelioid macrophages were detected. Morphometric measurement of Sirius red staining was used to assess the connective tissue response. The average thickness of connective tissue in alveolar regions was 0.10 ± 0.02, 0.09 ± 0.02, 0.10 ± 0.01, 0.48 ± 0.04, and 0.88 ± 0.19 μm for PBS and 1-h, 1-day, 7-day, and 1-mo postexposure groups, respectively. The results demonstrate that dispersed SWCNT are rapidly incorporated into the alveolar interstitium and that they produce an increase in collagen deposition.

Evaluation of biocompatibility of an experimental membrane for glucose sensors: the results of a prospective experimental controlled preclinical study involving laboratory animals

2017 ◽  
Vol 63 (4) ◽  
pp. 219-226 ◽  
Author(s):  
Yaroslav M. Stanishevskiy ◽  
Nadezhda P. Sachivkina ◽  
Yuriy V. Tarasov ◽  
Yury I. Philippov ◽  
Sergey A. Sokolov ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Membrane Surface ◽  
Glucose Sensors ◽  
Laboratory Animals ◽  
Skin Reactions ◽  
Inflammatory Reactions ◽  
Connective Tissue Capsule ◽  
Coated Membranes ◽  
Coated Membrane ◽  
Tissue Capsule

An increase in the accuracy of monitoring of glucose concentration indicators and an increase in the running time of glucose sensors are promising directions in the field of diabetology. One of the ways to extend the lifetime of a sensor is its complete implantation excluding direct communication with the skin surface. For effective long-term functioning in the patient’s body, the surface of an implantable sensor should be highly biocompatibile: it should not induce allergic and inflammatory reactions as well as the demarcation reaction (formation of a dense connective tissue capsule). Earlier, a group of authors developed a glucose-permeable membrane and a biocompatible coating comprising a complex of nadroparin with transesterified polyethylene glycol and γ-aminopropyl triethoxysilane, which formed a protein repellent hydrogel on the membrane surface. Aims. To evaluate the biocompatibility of the experimental coated membrane implanted into laboratory animals. Methods. The experimental prospective controlled study involved 60 laboratory animals (Wistar albino rats). The animals were divided into 3 groups of 20 animals each. Animals of each group were implanted with the standard, or experimental, or experimental coated membrane. After implantation, the skin condition in the implantation area was visually assessed for 90 days. After 90 days, the tissue condition around the implant was evaluated histologically. Results. No serious allergic or inflammatory reactions in the implantation area were detected in all three groups of animals within 90 days of the follow-up period. In the case of the experimental coated membrane, a significantly low score was graded based on visual assessment of the skin reactions. In the histological analysis, the tissue condition in the implantation area of the coated membranes was characterized by significantly lower density of a connective tissue capsule and the presence of vascularization areas at the contact between of the membrane surface and the surrounding tissue. Conclusion. In experimental animals, the tested coating significantly inhibits formation of a connective tissue capsule around the implant and reduces the intensity of skin reactions after implantation. Further clinical studies of coated membranes in humans are required to verify their biocompatibility.

Microsporidial Infection of English Sole, Parophrys vetulus

1969 ◽  
Vol 26 (8) ◽  
pp. 2215-2218 ◽  
Author(s):  
S. R. Wellings ◽  
L. E. Ashley ◽  
G. E. McArn
Keyword(s):  
Connective Tissue ◽  
Intermediate Layer ◽  
English Sole ◽  
Central Mass ◽  
Connective Tissue Capsule ◽  
Parophrys Vetulus ◽  
Tissue Capsule

Infection of two English sole, Parophrys vetulus, probably by Glugea hertwigi, is described. Numerous Glugea cysts (xenomas), measuring 0.5–1.0 mm in diameter, were located throughout the entire intestine distal to the stomach and including the pyloric caecae. A few cysts were located in the mesentery, pancreas, and liver. Microscopically, the cysts were composed of a hyaline connective tissue capsule, enclosing an intermediate layer of syncytium and a central mass of mature spores.

scholarly journals Tissue toxicity of root canal sealers

2004 ◽  
Vol 51 (3) ◽  
pp. 136-141 ◽  
Author(s):  
Mirjana Vujaskovic ◽  
Dragoljub Bacetic
Keyword(s):  
Connective Tissue ◽  
Root Canal ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Control Material ◽  
Tissue Sections ◽  
Subcutaneous Connective Tissue ◽  
Tissue Reactions ◽  
Root Canal Sealers ◽  
Observation Period

The aim of this study was to evaluate tissue response to root canal sealers Tubuliseal and Sealapex. The sealers were freshly mixed and injected in the dorsal subcutaneous connective tissue of 12 Wistar rats.The observation periods were 7 days, 21days and 60 days. Four operative areas were formed ( 2 for test sealers, Tubliseal or Sealapex and 2 for control material) on each animal. Tissue sections were taken from selected sites. Each section included skin, subcutaneous connective tissue and underlying muscle tissue.All blocks were processed with the use of standardized histological procedures.The tissue reactions were studied under light microscopy. Different grades of tissue reaction to the tested materials were recorded as mild, moderate or severe inflammation. After seven days both root canal sealers showed severe inflammatory reaction of connective tissue in experimental animals.Tubuliseal caused prolonged moderate and mild inflammation. Sealapex caused mild inflammation which diminished at the end of the observation period. The results of this study demonstrated that Sealapex was better tolerated by tissue than Tubliseal.

scholarly journals COMPARATIVE ULTRASTRUCTURAL CHARACTERISTICS OF THE EFFECT OF “LIGHTWEIGHT” AND “HEAVYWEIGHT” POLYPROPYLENE MESHES ON THE PRO-CESSES OF ANTERIOR ABDOMINAL WALL TISSUE REGENERATION WHEN PERFORMING RETROMUSCULAR ALLOGENEOPLASTY

2018 ◽  
Vol 17 (2) ◽  
pp. 76-83
Author(s):  
V. I. Pyatnochk ◽  
I. Ya. Dzyubanovsʹkyy ◽  
K. S. Volkov
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Anterior Abdominal Wall ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Ultrastructural Changes ◽  
Lightweight Mesh ◽  
Aseptic Inflammation ◽  
Ultrastructural Studies ◽  
Tissue Reactions

The ultrastructural changes of the tissues of musculo-aponeurotic layer of anterior abdominal wall after implantation of the “lightweight” and “heavyweight” polypropylene meshes into the retrometallic space were studied in the experiment. Significant differences of the tissue reactions at different periods of the experiment on the implanted material were evidenced. The presence of a moderately pronounced inflammatory tissue reaction was noticed on the 14th day of the study in the area of the implantation of the “lightweight” polypropylene mesh compared to the “heavyweight” one: the inflammatory response included acute manifestations of aseptic inflammation with a significant tissue response. On the 28th day of the experiment, in cases of implantation of the “lightweight” mesh, the stabilization of tissue reaction to the implant with reduction of inflammatory changes was observed, as evidenced by a significantly smaller number of leukocyte infiltration sites, a significant improvement in vascularization and development of collagen fibres around the mesh material with fibroblasts, which was not observed in the area of implantation of the “heavyweight” polypropylene mesh. The conducted morphological and ultrastructural studies have prioritized the use of “lightweight” meshes when choosing material to perform allohernioplasty.

scholarly journals Biomechanical Characteristics of Light Synthetic Materials for Hernioplasty (Experimental Study)

2018 ◽  
Vol 11 (3) ◽  
pp. 154-160 ◽  
Author(s):  
Boris Semenovich Sukovatykh ◽  
Andrey Alexeevich Netiaga ◽  
Yuri Yurievich Blinkov ◽  
Yuri Yurievich Polevoy ◽  
Valery Anatolievich Zhukovskii
Keyword(s):  
Abdominal Wall ◽  
Experimental Studies ◽  
Biomechanical Properties ◽  
Breaking Load ◽  
Control Series ◽  
Connective Tissue Capsule ◽  
Synthetic Materials ◽  
Significant Difference ◽  
Series Of Experiments ◽  
Tissue Capsule

Importance of the topic. One of the directions of improving hernioplasty efficiency with the use of light synthetic materials is including the strengthening zones in their structure. Purpose. To compare functional effectiveness of the light and the light strengthened polypropylene endoprostheses for hernioplasty on the base of studying biomechanical characteristics of anterior abdominal wall tissues after their implantation in experiment. Materials and methods. Experimental studies were conducted on 36 rabbits of “chinchilla”  breed. All animals were divided into 2 series of experiments. In the first (control) series the light polypropylene endoprosthesis was implanted, while in the second (experimental) series there was used a new cancellated  endoprosthesis from monofilamentous polypropylene, consisting of the basic and strengthened zones in the form of horizontally located, alternating with each other monothreads of the higher thickness. Pathomorphological changes and biomechanical properties of the abdominal wall tissues were studied macroscopically in the experiment by implantation of endoprostheses in onlay position on the 7th, 30th and 90th  days. Results and their discussion. There was proved that in direction of the loop column as well as in the  loop row after implantation of the studied materials breaking load increased from the 7th to the 90th day of experiment, that was related to the formation of the connective tissue capsule around materials. The light strengthened endoprosthesis was more durable and stronger in 1,6 times compare to the light analog at all stages of observation. Along the loop column there was no significant difference detected. In both series of experiment in the direction of the loop column as well as in  loop row the reduction of sample lengthening took place. Along the loop column indicators of lengthening, characterizing elasticity of the prosthesis aponeurosis, in the series of the light strengthened endoprosthesis were 1,4-1,6 times lower than in its light analog. Along the loop row there was no significant difference of indicators. Conclusion. Introduction of the strengthened zones in a light endoprosthesis increases its durability with preservation of the material’s elasticity.

scholarly journals Bilateral adrenal-renal fusion: A radiological diagnosis

2019 ◽  
Vol 5 (2) ◽  
pp. 20180108
Author(s):  
Rebecca Bamford ◽  
Josephine Bretherton ◽  
Nicola Rosenfelder ◽  
James Bell
Keyword(s):  
Connective Tissue ◽  
Embryonic Development ◽  
Histological Examination ◽  
Adrenal Glands ◽  
Normal Anatomy ◽  
True Incidence ◽  
Connective Tissue Capsule ◽  
Mesenchymal Tissue ◽  
Renal Fascia ◽  
Tissue Capsule

In normal anatomy, the kidneys and adrenal glands are contained within the renal fascia and separated by a connective tissue capsule derived from mesenchymal tissue. Incomplete encapsulation can occur during embryonic development, resulting in adrenal-renal fusion. The true incidence of this developmental anomaly is unknown, as it has primarily been described in the literature following incidental detection on surgical or histological examination. We report the first documented case of bilateral adrenal-renal fusion, diagnosed radiologically.

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