scholarly journals Phenotyping Peanut Genotypes for Drought Tolerance

2016 ◽  
Vol 43 (1) ◽  
pp. 36-48 ◽  
Author(s):  
J.M. Luis ◽  
P. Ozias-Akins ◽  
C.C. Holbrook ◽  
R.C. Kemerait ◽  
J.L. Snider ◽  
...  

ABSTRACT Drought and heat stress can result in aflatoxin contamination of peanuts especially when this occurs during the last three to six wk of the growing season. Identifying drought-tolerant genotypes may aid in development of peanuts that are less susceptible to aflatoxin contamination. Research was conducted to phenotype seven peanut genotypes based on their response to drought stress. Six peanut genotypes that have exhibited lower aflatoxin and/or drought tolerance in previous researches (Tifguard, Tifrunner, Florida-07, PI 158839, NC 3033, C76-16) were compared to an aflatoxin-susceptible genotype, A72. The phenotyping methods included visual ratings, chlorophyll fluorescence (PIABS, ϕEO, and Fv/Fm), SPAD chlorophyll meter reading (SCMR), normalized difference vegetation index (NDVI), canopy temperature (CT), canopy temperature depression (CTD), and pod yield. Based on these traits, Tifguard and Tifrunner exhibited greater drought tolerance mechanisms than the other genotypes and may be good candidates to be incorporated in future drought tolerance studies. After the aflatoxin content of the different genotypes was measured, aflatoxin contamination showed high correlations with visual ratings (0.85), CTD (0.81), NDVI (0.79), and CT (0.73), and moderate correlations with Fv/Fm (0.62) and SCMR (0.57) (P ≥ 0.05). These easily measurable, rapid and cost-effective phenotyping methods may be used as alternative to more tedious and costly methods of identifying genotypes that are less susceptible to aflatoxin contamination. Using a combination of these methods is beneficial but not always practical. The combined use of visual ratings, CTD and NDVI is advised for initial evaluation of drought tolerance in peanut genotypes.

2000 ◽  
Vol 27 (2) ◽  
pp. 45-48 ◽  
Author(s):  
C. C. Holbrook ◽  
C. K. Kvien ◽  
K. S. Rucker ◽  
D. M. Wilson ◽  
J. E. Hook ◽  
...  

Abstract Peanuts become contaminated with aflatoxins when subjected to prolonged periods of heat and drought stress. The effect of drought tolerance on aflatoxin contamination is not known. The objectives of this research were to evaluate preharvest aflatoxin contamination in peanut genotypes known to have drought tolerance and to determine the correlation of drought tolerance characteristics with aflatoxin contamination. Twenty genotypes with different levels of drought tolerance were grown in Yuma, AZ (a desert environment) and under rain-protected shelters in Tifton, GA. Two drought-tolerant genotypes (PI 145681 and Tifton 8) and an intolerant genotype (PI 196754) were selected for further examination in a second experiment with two planting dates in 1997 at Tifton. Drought and heat stress conditions were imposed for the 40 d preceding harvest. The drought-intolerant genotype had greater preharvest aflatoxin contamination than Florunner (the check cultivar) in the tests conducted in 1997. Both drought-tolerant genotypes had less preharvest aflatoxin contamination than Florunner in these tests. Significant positive correlations were observed between aflatoxin contamination and leaf temperature and between aflatoxin contamination and visual stress ratings. Leaf temperature and visual stress ratings are less variable and less expensive to measure than aflatoxin contamination. Leaf temperature and visual stress ratings maybe useful in indirectly selecting for reduced aflatoxin contamination in breeding populations.


2018 ◽  
Vol 8 ◽  
pp. 91-100
Author(s):  
Belete Berhanu ◽  
Ethiopia Bisrat

Ethiopia is endowed with water and has a high runoff generation area compared to many countries, but the total stored water only goes up to approximately 36BCM. The problem of water shortage in Ethiopia emanates from the seasonality of rainfall and the lack of infrastructure for storage to capture excess runoff during flood seasons. Based on this premise, a method for a syndicate use of topography, land use and vegetation was applied to locate potential surface water storing sites. The steady-state Topographic Wetness Index (TWI) was used to represent the spatial distribution of water flow and water stagnating across the study area and the Normalized Difference Vegetation Index (NDVI) was used to detect surface water through multispectral analysis. With this approach, a number of water storing sites were identified in three categories: primary sources (water bodies based), secondary sources (Swampy/wetland based) and tertiary sources (the land based). A sample volume analysis for the 120354 water storing sites in category two, gives a 44.92BCM potential storing capacity with average depth of 4 m that improves the annual storage capacity of the country to 81BCM (8.6 % of annual renewable water sources). Finally, the research confirmed the TWI and NDVI based approach for water storing sites works without huge and complicated earth work; it is cost effective and has the potential of solving complex water resource challenges through spatial representation of water resource systems. Furthermore, the application of remote sensing captures temporal diversity and includes repetitive archives of data, enabling the monitoring of areas, even those that are inaccessible, at regular intervals.


Fire ◽  
2021 ◽  
Vol 4 (4) ◽  
pp. 68
Author(s):  
Sarah A. Lewis ◽  
Peter R. Robichaud ◽  
Andrew T. Hudak ◽  
Eva K. Strand ◽  
Jan U. H. Eitel ◽  
...  

As wildland fires amplify in size in many regions in the western USA, land and water managers are increasingly concerned about the deleterious effects on drinking water supplies. Consequences of severe wildfires include disturbed soils and areas of thick ash cover, which raises the concern of the risk of water contamination via ash. The persistence of ash cover and depth were monitored for up to 90 days post-fire at nearly 100 plots distributed between two wildfires in Idaho and Washington, USA. Our goal was to determine the most ‘cost’ effective, operational method of mapping post-wildfire ash cover in terms of financial, data volume, time, and processing costs. Field measurements were coupled with multi-platform satellite and aerial imagery collected during the same time span. The image types spanned the spatial resolution of 30 m to sub-meter (Landsat-8, Sentinel-2, WorldView-2, and a drone), while the spectral resolution spanned visible through SWIR (short-wave infrared) bands, and they were all collected at various time scales. We that found several common vegetation and post-fire spectral indices were correlated with ash cover (r = 0.6–0.85); however, the blue normalized difference vegetation index (BNDVI) with monthly Sentinel-2 imagery was especially well-suited for monitoring the change in ash cover during its ephemeral period. A map of the ash cover can be used to estimate the ash load, which can then be used as an input into a hydrologic model predicting ash transport and fate, helping to ultimately improve our ability to predict impacts on downstream water resources.


Drones ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. 43 ◽  
Author(s):  
Sathishkumar Samiappan ◽  
Lee Hathcock ◽  
Gray Turnage ◽  
Cary McCraine ◽  
Jonathan Pitchford ◽  
...  

Wildfires can be beneficial for native vegetation. However, wildfires can impact property values, human safety, and ecosystem function. Resource managers require safe, easy to use, timely, and cost-effective methods for quantifying wildfire damage and regeneration. In this work, we demonstrate an approach using an unmanned aerial system (UAS) equipped with a MicaSense RedEdge multispectral sensor to classify and estimate wildfire damage in a coastal marsh. We collected approximately 7.2 km2 of five-band multispectral imagery after a wildfire event in February 2016, which was used to create a photogrammetry-based digital surface model (DSM) and orthomosaic for object-based classification analysis. Airborne light detection and ranging data were used to validate the accuracy of the DSM. Four-band airborne imagery from pre- and post-fire were used to estimate pre-fire health, post-fire damage, and track the vegetation recovery process. Immediate and long-term post-fire classifications, area, and volume of burned regions were produced to track the revegetation progress. The UAS-based classification produced from normalized difference vegetation index and DSM was compared to the Landsat-based Burned Area Reflectance Classification. Experimental results show the potential of using UAS and the presented approach compared to satellite-based mapping in terms of classification accuracies, turnaround time, and spatial and temporal resolutions.


2018 ◽  
Vol 10 (4) ◽  
pp. 575-583 ◽  
Author(s):  
Fereshteh JOKAR ◽  
Rahmatollah KARIMIZADEH ◽  
Asad MASOUMIASL ◽  
Reza AMIRI FAHLIANI

Durum wheat (Triticum durum L.) is used for the preparation of multiple food products, including pasta and bread. Its production is restricted due to diverse environmental stresses i.e. drought and heat stress. Here, comparative analysis of durum wheat varieties was done by studying canopy temperature depression (CTD) and chlorophyll content (CHL), yield and yield contributing traits to evaluate their performance under stress and low stress conditions. Twelve durum wheat genotypes were studied under stressful and low-stress conditions in Gachsaran region of Iran. CTD and CHL were measured at two stages, from the emergence of fifty percent of inflorescence (ZGS 54) to watery ripe stage (ZGS 71). According to stress tolerance index (STI), mean productivity (MP) and geometric mean productivity (GMP) indices, genotype G10 exhibited the most, while genotype G6, the least relative tolerance, respectively. Based on MP and GMP, genotype G10 was found to be drought tolerant, while genotype G2 displayed the lowest amount of MP and GMP. Therefore these genotypes are recommended to be used as genitors in artificial hybridization for improvement of drought tolerance in other cultivars. All indices had high correlation with grain yield under stress and non-stress condition, indicating more suitability of these indices for selection of resistant genotype. Results of the present study showed that among drought tolerance indices, harmonic mean (HM), GMP, CTD and modified STI index (K2STI) can be used as the most suitable indicators for screening drought tolerant cultivars.


2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Manuela Signorini ◽  
Anna-Sofie Stensgaard ◽  
Michele Drigo ◽  
Giulia Simonato ◽  
Federica Marcer ◽  
...  

Various ticks exist in the temperate hilly and pre-alpine areas of Northern Italy, where Ixodes ricinus is the more important. In this area different tick-borne pathogen monitoring projects have recently been implemented; we present here the results of a twoyear field survey of ticks and associated pathogens, conducted 2009-2010 in North-eastern Italy. The cost-effectiveness of different sampling strategies, hypothesized a posteriori based on two sub-sets of data, were compared and analysed. The same two subsets were also used to develop models of habitat suitability, using a maximum entropy algorithm based on remotely sensed data. Comparison of the two strategies (in terms of number of ticks collected, rates of pathogen detection and model accuracy) indicated that monitoring at many temporary sites was more cost-effective than monthly samplings at a few permanent sites. The two model predictions were similar and provided a greater understanding of ecological requirements of I. ricinus in the study area. Dense vegetation cover, as measured by the normalized difference vegetation index, was identified as a good predictor of tick presence, whereas high summer temperatures appeared to be a limiting factor. The study suggests that it is possible to obtain realistic results (in terms of pathogens detection and development of habitat suitability maps) with a relatively limited sampling effort and a wellplanned monitoring strategy.


Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 192 ◽  
Author(s):  
Abdulwahab S. Shaibu ◽  
Clay Sneller ◽  
Babu N. Motagi ◽  
Jackline Chepkoech ◽  
Mercy Chepngetich ◽  
...  

In order to integrate genomics in breeding and development of drought-tolerant groundnut genotypes, identification of genomic regions/genetic markers for drought surrogate traits is essential. We used 3249 diversity array technology sequencing (DArTSeq) markers for a genetic analysis of 125 ICRISAT groundnut mini core collection evaluated in 2015 and 2017 for genome-wide marker-trait association for some physiological traits and to determine the magnitude of linkage disequilibrium (LD). Marker-trait association (MTA) analysis, probability values, and percent variation modelled by the markers were calculated using the GAPIT package via the KDCompute interface. The LD analysis showed that about 36% of loci pairs were in significant LD (p < 0.05 and r2 > 0.2) and 3.14% of the pairs were in complete LD. The MTAs studies revealed 20 significant MTAs (p < 0.001) with 11 markers. Four MTAs were identified for leaf area index, 13 for canopy temperature, one for chlorophyll content and two for normalized difference vegetation index. The markers explained 20.8% to 6.6% of the phenotypic variation observed. Most of the MTAs identified on the A subgenome were also identified on the respective homeologous chromosome on the B subgenome. This could be due to a common ancestor of the A and B genome which explains the linkage detected between markers lying on different chromosomes. The markers identified in this study can serve as useful genomic resources to initiate marker-assisted selection and trait introgression of groundnut for drought tolerance after further validation.


PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0242472
Author(s):  
A. S. Nehe ◽  
M. J. Foulkes ◽  
I. Ozturk ◽  
A. Rasheed ◽  
L. York ◽  
...  

Bread wheat (Triticum aestivum L) is one of the three main staple crops worldwide contributing 20% calories in the human diet. Drought stress is the main factor limiting yields and threatening food security, with climate change resulting in more frequent and intense drought. Developing drought-tolerant wheat cultivars is a promising way forward. The use of holistic approaches that include high-throughput phenotyping and genetic markers in selection could help in accelerating genetic gains. Fifty advanced breeding lines were selected from the CIMMYT Turkey winter wheat breeding program and studied under irrigated and semiarid conditions in two years. High-throughput phenotyping was done for wheat crown root traits and canopy senescence dynamics using vegetation indices (green area using RGB images and Normalized Difference Vegetation Index using spectral reflectance). In addition, genotyping by KASP markers for adaptability genes was done. Overall, under semiarid conditions yield reduced by 3.09 t ha-1 (-46.8%) compared to irrigated conditions. Genotypes responded differently under drought stress and genotypes 39 (VORONA/HD24-12//GUN/7/VEE#8//…/8/ALTAY), 18 (BiII98) and 29 (NIKIFOR//KROSHKA) were the most drought tolerant. Root traits including shallow nodal root angle under irrigated conditions and root number per shoot under semiarid conditions were correlated with increased grain yield. RGB based vegetation index measuring canopy green area at anthesis was better correlated with GY than NDVI was with GY under drought. The markers for five established functional genes (PRR73.A1 –flowering time, TEF-7A –grain size and weight, TaCwi.4A - yield under drought, Dreb1- drought tolerance, and ISBW11.GY.QTL.CANDIDATE- grain yield) were associated with different drought-tolerance traits in this experiment. We conclude that–genotypes 39, 18 and 29 could be used for drought tolerance breeding. The trait combinations of canopy green area at anthesis, and root number per shoot along with key drought adaptability makers (TaCwi.4A and Dreb1) could be used in screening drought tolerance wheat breeding lines.


2021 ◽  
Vol 32 (2) ◽  
pp. 96
Author(s):  
Dhuha S. Al-Khafaji ◽  
Asraa Khtan Abdulkareem ◽  
Qusai Y. Al-Kubaisi

To improve the management of water resources in Iraq, there are several methods, including the use of rainwater harvesting techniques. In this study, the Digital Elevation Model (DEM) and Landsat satellite imagery were used under the GIS environment to identify the suitable zones for rainwater harvesting. The accomplishment of rainwater harvesting systems strongly depends on their technical designing and identifying the suitable sites. Six criteria have been used to identify the rainwater harvesting sites in the Diyala governorate. The procedure of identifying the suitable sites for rainwater harvesting was applied twice for the Diyala governorate. Firstly, it was applied by using the criteria of rainfall, slope, stream order, distance to roads, and land use, and secondly, rainfall, slope, stream order, distance to roads, and Normalized Difference Vegetation Index (NDVI) criteria were used for this purpose. As a result, the study area was divided into three suitability zones: low, moderate, and high according to the specific criteria that were used to identify the rainwater harvesting suitable sites. It was found that in the application of land use criterion the low suitability zone represents 26%, 58% represents the moderate, and 16% for the high suitability zone, while in the method of NDVI it was found that 29% represents the zone that has low suitability, 57% represents the moderate, and 14% represents the high suitability zone. The compared results led to conclude that the land use is the most influential criterion for identifying the rainwater harvesting suitability sites and found that most of the Eastern parts of Diyala governorate are promising areas for rainwater harvesting and ArcGIS is a very useful, time-saving, and cost-effective tool for identifying the rainwater harvesting suitable sites.


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