THE INFLUENCE OF THE PHYSIOLOGICAL STATE OF RABBITS ON HEMATOLOGICAL INDICATORS WITH CHANGES IN THE AREA OF THE PLANTARY SURFACE OF THE FEET

The influence of the physiological state of rabbits (pregnancy and lactation) on the hematological parameters of blood during changes in the area of the plantar surface of the feet of the hind limbs in conditions of cage keeping was studied. In the body of pregnant females, with the appearance of signs of hyperkeratosis and alopecia in the studied area of the feet, an increase in hemoglobin level occurs, an increase in MCH (mean corpuscular hemoglobin), MCV (Mean Corpuscular Volume), MCHC (corpuscular hemoglobin concentration) occurs, followed by a decrease in the development of hemorrhagic changes. The level of red blood cells has slight fluctuations. In lactating females, with the occurrence of alopecia and hyperkeratosis, an increase in the level of erythrocytes and hematocrit occurs with a simultaneous decrease in hemoglobin, and against the background of the development of hemorrhages, these indicators decrease, a decline in the values of MCH, MCHS and an increase in MCV are recorded. Regardless of the physiological state of the animal and changes in the area of the feet, the erythrocyte sedimentation rate (ESR) value and the level of leukocytes fluctuate within the limits of physiological values, which indicates the absence of an inflammatory process in the body. he revealed hematological changes indicate the development of adaptive processes in the event of hyperkeratosis and alopecia in the area of the plantar surface of the feet at various physiological conditions of rabbits with subsequent decompensating against the background of hemorrhagic changes.

Download Full-text

Age-Sex Related in Hematological Values of Sandalwood Pony Horses (Equus caballus) in East Sumba, NTT

Journal of Veterinary and Animal Sciences ◽

10.24843/jvas.2020.v03.i02.p06 ◽

2020 ◽

Vol 3 (2) ◽

Author(s):

Cynthia Dewi Gaina ◽

Antin Y. N. Widi ◽

Agus Saputra

Keyword(s):

Hematological Parameters ◽

Age Groups ◽

Hemoglobin Concentration ◽

Physiological Status ◽

Published Data ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Hematological Indicators ◽

Age And Sex ◽

Hematological Values

Normal hematological values need to be defined for each category of horse, like age and sex to obtain precise diagnostic results. No published data on hematology exist for Sandalwood horses that are extensively reared in East Sumba, NTT. The main objective of this research was to compare the hematological characteristic of horse group based on age and sex. Blood samples from sixteen horses were collected based on sex into male (n=6), female (n=10) and age groups of 2-3 years (n=4) and 6-10 years old (n=12). About 3 ml of blood sample was collected aseptically from jugular vein of each of these animals in sterile vacutainer tube. The following variables were measured: erythrocyte, leucocyte, platelet, hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and mean platelet volume (MPV). These hematological parameters were calculated with the automatic hematology analyzer at Health Laboratory in Kupang, NTT. This result was analyzed by using student T-test and analysis of variance. These results would contribute to better understanding of the hematological indicators for estimating the physiological status of Sandalwood horse in East Sumba as it can be used as valuable information of its health physiological status.

Download Full-text

Physiological and hematological parameters of sheep reared in the tropics and subtropics

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-12204 ◽

2021 ◽

Vol 73 (3) ◽

pp. 622-630

Author(s):

L. Seixas ◽

V. Peripolli ◽

D. A.E. Façanha ◽

V. Fischer ◽

C.H.E.C. Poli ◽

...

Keyword(s):

Reference Values ◽

Hematological Parameters ◽

Physiological State ◽

Hemoglobin Concentration ◽

Time Of Day ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Normal Range ◽

The Tropics ◽

Species Being

ABSTRACT This study aimed to determine the physiological and hematological parameters of sheep in Brazil. Therefore, 5,081 observations were used from previous experiments with animals from different Brazilian regions with the time of day, gender, breed, age, physiological characteristics, hematological and climatic variables. The animals were classified according to their stress level, and only data of non-stressed animals were used to calculate the reference values. Respiratory rate was the parameter that most differed from the normal range, with values above the limits usually reported for the species, being higher in commercial wool sheep. For hematological variables, differences were observed between the present results and the previously reported values in the literature. A higher number of red blood cells (RBC), lower hemoglobin and mean corpuscular hemoglobin concentration (MCHC) were observed in young animals. A higher number of RBC and MCHC were noted in sheep crossed with local breeds. Considering that physiological and hematological parameters are indicators of the animal's pathological or physiological state, for a correct interpretation of the results, the use of reference values based on animals with similar racial characteristics and subjected to similar environmental conditions is recommended.

Download Full-text

Blood Donation in Nigeria: Standard of the Donated Blood

Journal of Laboratory Physicians ◽

10.4103/0974-2727.105589 ◽

2012 ◽

Vol 4 (02) ◽

pp. 094-097 ◽

Cited By ~ 4

Author(s):

Nwogoh Benedict ◽

Awodu Omolade Augustina ◽

Bazuaye Godwin Nosakhare

Keyword(s):

Blood Donation ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Mean Cell Volume ◽

Benin City ◽

Cell Counts ◽

Significant Difference ◽

The Mean

ABSTRACT Objective: The objective was to determine the basic hematological parameters of remunerated blood donors in Benin City and to compare them with those of voluntary donors. Materials and Methods: This is a prospective study conducted in a tertiary health facility in Benin City. Pretransfusion samples were obtained from blood bags after gentle mixing and analyzed for hematological parameters. Samples were analyzed using the hematology autoanalyzer MODEL SYSMEX KN21. Result: A total of 215 samples were obtained comprising 160 remunerated (paid) and 55 voluntary donor samples. In the paid donors, the mean hemoglobin concentration (Hb) and hematocrit (HCT) 7.7±2.9 and 28.8±8.5 respectively. This was significantly lower than those of voluntary donors who had 13.9±1.2 and 42.2±3.3 with P < 0.001. The mean values of the red cell counts (RBC), white cell counts (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in paid donors as P-values were <0.001. MCV was significantly low but not compared to the other parameters as P=0.04. There was no significant difference in the platelet count. Conclusion: Paid donors in Benin City have significantly lower hematological parameters than controls.

Download Full-text

Statin and anemia in chronic kidney disease (CKD): an experimental study

MATEC Web of Conferences ◽

10.1051/matecconf/201819707003 ◽

2018 ◽

Vol 197 ◽

pp. 07003 ◽

Cited By ~ 1

Author(s):

P N Cahyawati ◽

A A S A Aryastuti ◽

M B T Ariawan ◽

N Arfian ◽

Ngatidjan Ngatidjan

Keyword(s):

Chronic Kidney Disease ◽

Kidney Disease ◽

Hematological Parameters ◽

Treatment Options ◽

White Blood Cells ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Simvastatin Group ◽

Group 5

Anemia is a common feature of chronic kidney disease (CKD). The current management of patients with anemia in CKD is controversial. These make anemia is one of the leading causes poor outcome in CKD patients. Our study focus to assess the effect of statin on hemoglobin level with an experimental model of CKD. We perform the animal model of CKD by subtotal nephrectomy procedure. A total of 20 male swiss was used in these study. All the animal divided into 4 groups: sham group (S), nephrectomy (Nx), simvastatin group 5 mg/kgBB (SV5), and simvastatin group 10 mg/kgBB (SV10). Hematological parameters (hemoglobin (Hb), white blood cells (WBC), red blood cell (RBC), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)), and serum creatinine are measured at the end of the study. The hemoglobin levels in the Ux group decreased significantly compared with the S group (11.02 ± 0.46 vs 14.3 ± 0.15, p<0.0001). While in the SV group, statin administration led to an increase in hemoglobin levels compared with the Ux group but not statistically significant (SV5: 13.26±0.34; Sv10: 12.68±0.26). These results suggest that administration of statins in CKD may improve the condition of anemia by an unknown mechanism, while still requiring other treatment options to maximize the effect of therapy.

Download Full-text

Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

The Scientific World JOURNAL ◽

10.1155/2014/491415 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Madhusmita Das ◽

Pravati Kumari Mahapatra

Keyword(s):

Blood Cell ◽

Blood Serum ◽

Blood Cells ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Reference Ranges ◽

Corpuscular Hemoglobin ◽

Differential Leukocyte Count ◽

Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

Download Full-text

Hematological parameters of Hoplias malabaricus (Characiformes: Erythrinidae) parasitized by Monogenea in lagoons in Pirassununga, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612013000400003 ◽

2013 ◽

Vol 22 (4) ◽

pp. 457-462 ◽

Cited By ~ 4

Author(s):

Lincoln Lima Corrêa ◽

Letícia Cucolo Karling ◽

Ricardo Massato Takemoto ◽

Paulo Sérgio Ceccarelli ◽

Marlene Tiduko Ueta

Keyword(s):

Condition Factor ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Good Health ◽

Blood Parameters ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Hoplias Malabaricus ◽

Relative Condition Factor ◽

The Mean

Hematology is an important pathological and diagnostic tool. This paper describes the hematological parameters of 76 specimens of Hoplias malabaricus infested with monogenean parasites, which were collected from two lagoons. The hematological parameters included: erythrocyte count (Er), microhematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood parameters showed no significant changes associated with the infestation. Water temperature was found to be negatively correlated with MCV and Hct, with values of rs= −0.52, p<0.0001 and rs= −0.48, p<0.0001, respectively. The mean Relative Condition Factor was Kn=1.01, indicating good health conditions of the fish in these lagoons. No correlation was found between the monogenean infestation and the blood parameters, or between the environmental factors and the monogeneans.

Download Full-text

Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000400007 ◽

2007 ◽

Vol 50 (4) ◽

pp. 619-626 ◽

Cited By ~ 21

Author(s):

Nilton Massuo Ishikawa ◽

Maria José Tavares Ranzani-Paiva ◽

Julio Vicente Lombardi ◽

Cláudia Maris Ferreira

Keyword(s):

Blood Cell ◽

Cell Count ◽

Oreochromis Niloticus ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Control Group ◽

Mean Corpuscular Hemoglobin ◽

Blood Cell Count ◽

Corpuscular Hemoglobin ◽

Set Up

Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

Download Full-text

Is erythrocyte size a strategy to avoid hypoxia in Wiegmann’s Torquate Lizards (Sceloporus torquatus)? Field evidence

Canadian Journal of Zoology ◽

10.1139/cjz-2014-0265 ◽

2015 ◽

Vol 93 (5) ◽

pp. 377-382 ◽

Cited By ~ 11

Author(s):

Juan C. González-Morales ◽

Erendira Quintana ◽

Hector Díaz-Albiter ◽

Palestina Guevara-Fiore ◽

Victor Fajardo

Keyword(s):

Physiological Response ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Mean Corpuscular Hemoglobin Concentration ◽

Corpuscular Hemoglobin ◽

Serum Electrolytes ◽

Blood Samples ◽

Field Evidence ◽

Different Altitudes

This study examined changes in certain hematological parameters in a reptilian model naturally exposed to altitude-associated hypoxia. Four populations of the Mexican lizard Sceloporus torquatus Wiegmann, 1828 (Wiegmann’s Torquate Lizard) from different altitudes were sampled to evaluate erythrocyte count (Erc), hematocrit (Hct), mean corpuscular hemoglobin concentration (MCHC), and erythrocyte size (Ers). Blood was also assayed to determine hemoglobin ([Hb]), glucose, lactate, and electrolyte concentrations. Erc was performed using a Neubauer hemocytometer. Hct was calculated as percentage of packed cell volume by centrifuging blood samples. [Hb] was determined using a Bausch and Lomb Spectronic colorimeter. MCHC was calculated with the formula 100 × [Hb]/Hct. Ers was calculated from blood smear microphotographs analyzed with the Sigma Scan Pro software. Values of serum electrolytes (sodium (Na+), potassium (K+), and calcium (Ca2+)), pH, glucose, and lactate from blood samples were obtained through a blood electrolyte analyzer. Highland populations of S. torquatus exhibited a significant increase in Erc, Hct, Ers, and [Hb]. In contrast, MCHC showed no correlation with altitude. Additionally, significant differences in lactate, Na+, K+, and Ca2+ were observed in highland populations. In general, we found that most hematological parameters were significantly different among lizard populations from different altitudes. This is the first study to report changes in Ers in relation to altitude, which could be a physiological response to hypoxia.

Download Full-text

Impact of Camel's Milk on Aluminum Chloride (Alcl3) - Induced Toxicity in Rats

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2782 ◽

2019 ◽

Vol 16 (3) ◽

pp. 669-679

Author(s):

Hala A. Abdolhaleem ◽

Magda Abd Elaziz ◽

Mostafa M. Bashandy ◽

Wafai Z. A. Mikhail

Keyword(s):

Aluminum Chloride ◽

Histopathological Examination ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Negative Control ◽

Camel Milk ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Renal Functions ◽

Camel's Milk

The present study was carried out to evaluate the efficiency of camel's milk to ameliorate the toxicity of aluminum chloride AlCl3 on some hematological parameters; hepatic,renal functions andlipids profile; as well ashistopathological alterations of some organs. Forty rats (8 / group) were divided into 5 treatment groups:Group1: Normal rats (negative control); Group2: AlCl3induced toxicity rats (positive control); Group3: AlCl3induced toxicity rats fed with raw camel milk; Group4: AlCl3 induced toxicity rats fed with heat treated camel milk; andGroup5: AlCl3 induced toxicity rats fed with sweet acidophilus camel milk.Rats were treatedby 5ml camel’s milk 10 min before the administration of 1 ml AlCl3 (0.5 mg / kgbody weight); and had their respective doses daily for 30 successive days orally. AlCl3 oral administration resulted in a significant decrease in red blood cells count (RBC's), significant increase in mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH); while hemoglobin (Hb), hematocrite (Hct), platelets(plt), reticulocytes (Ret), mean corpuscular hemoglobin concentration (MCHC) did not revealed significant changes; the obtained anemia was macrocytic normochromic. The lipids profile; hepatic and renal functions showed non significant changes between different groups; however, histopathological examination showed variable alterations of varying severity in some organs; besides their response to camel's milk administration. Camel’s milk administration in groups 3, 4, 5 alleviated the toxic effect of AlCl3 with variable degrees between different groups.

Download Full-text

Increasing running volume elicits hematological changes in trained endurance runners: a case study (El aumento del volumen de entrenamiento de carrera induce alteraciones hematológicas en corredores entrenados: un estudio de caso)

Retos ◽

10.47197/retos.v0i35.59237 ◽

2018 ◽

pp. 117-120

Author(s):

José Augusto Rodrigues dos Santos

Keyword(s):

Hemoglobin Concentration ◽

Mean Corpuscular Hemoglobin ◽

Corpuscular Hemoglobin ◽

Endurance Running ◽

Mean Cell Volume ◽

Endurance Runners ◽

Hematological Indicators ◽

Hematological Changes ◽

Trained Runners

Abstract. Background: Endurance running training induces several hematological changes that increase the capture, transport and delivery of the oxygen to the exercising muscles. Objective: This study aimed to verify how a dramatic increase in running volume induced new alterations in several hematological indicators in previously trained endurance runners. Methods: Three subjects (PL: 26 years, 169,5 cm; HP, 27 years, 167,9 cm; MC, 27 years, 180,7 cm) running 10-12 km/day, increased their running volume to prepare the participation in a 100-km ultra-marathon. New training program included 10-12 training sessions per week, totalizing 200-260 km. Average daily running volume was 35.8±6.2 km. The parameters analyzed were: hemoglobin, erythrocytes, hematocrit, mean cell volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), leukocytes, neutrophils, eosinophils, lymphocytes, and monocytes. Results: Erythrocyte count, hemoglobin, and hematocrit decreased 6.5%, 5.1% and 6.7%, respectively for the average of the three runners. Leukocytes, neutrophils, eosinophils, lymphocytes, and monocytes showed different alterations among participants with all the values remaining within normal reference values. Conclusion: Well-trained runners show further hematological alterations when training volume is dramatically increased, which can be seen as the specific adaptation to the new training level. It seems that hemogram is more sensitive than leucogram to the increase in running volume.Resumen. Antecedentes: El entrenamiento de resistencia aeróbica induce varios cambios hematológicos que aumentan la captura, el transporte y la entrega del oxígeno a los músculos en ejercicio. Objetivo: El objetivo de este estudio fue verificar cómo un aumento dramático en el volumen de corrida indujo nuevas alteraciones en varios indicadores hematológicos en corredores previamente entrenados. Métodos: Tres sujetos (PL: 26 años, 169,5 cm, HP, 27 años, 167,9 cm, MC, 27 años, 180,7 cm) corriendo 10-12 km / día, aumentaron su volumen de carrera para preparar la participación en un ultra maratón de 100 km. El nuevo programa de entrenamiento incluyó 10-12 sesiones de entrenamiento por semana, totalizando 200-260 km. El promedio diario de volumen de carrera fue de 35,8 ± 6,2 km. Los parámetros analizados fueron hemoglobina, eritrocitos, hematocrito, volumen celular medio (MCV), hemoglobina corpuscular media (MCH), concentración media de hemoglobina corpuscular (MCHC), leucocitos, neutrófilos, eosinofilos, linfocitos y monocitos. Resultados: El recuento de eritrocitos, la hemoglobina y el hematocrito disminuyeron 6,5%, 5,1% y 6,7%, respectivamente, para el promedio de los tres corredores. Los leucocitos, neutrófilos, eosinofilos, linfocitos y monocitos mostraron diferentes alteraciones entre los participantes con todos los valores restando dentro de los valores normales de referencia. Conclusión: Corredores bien entrenados muestran alteraciones hematológicas adicionales cuando el volumen de entrenamiento aumenta drásticamente, lo que puede ser visto como la adaptación específica al nuevo nivel de entrenamiento. Parece que el hemograma es más sensible que el leucograma al aumento en el volumen de entrenamiento de carrera.

Download Full-text