Phytochemical Screening, Phenolic Compounds Content, Antioxidant, Anti-inflammatory, and Antimicrobial Properties of Pachylobus balsamifera Guillaum from Gabon

The goal of this work is to evaluate the antioxidant anti-inflammatory properties, the phenolic compounds content, and antimicrobial potential of water–acetone, water–ethanol, and water extracts of Pachylobus balsamifera. The phenolic compounds content was evaluated to estimate their effect on the antioxidant, anti-inflammatory, and antimicrobial potential of the plant. Antioxidant activities were examined by DPPH (2,2-diphenyl-1- picrylhydrazyl) and ABTS (2,2′-azino-bis) assays. Antiinflammatory activity was determined by the proteins denaturation inhibition method. All plant extracts were evaluated against six reference strains, eleven clinical isolates, and two fungal strains. Phenols content was highest in the water–acetone and water–ethanol extracts. The water extract showed strong anti-inflammatory effect. The water–acetone extract presented a strong antioxidant property and the highest antimicrobial activities against Pseudomonas aeruginosa, Acinetobacter baumannii, Salmonella Sp., and Neisseria meningitidis. The tested microorganisms showed sensitivity to all extracts of the plant with the exception of Escherichia coli 105182 CIP, Listeria innocua LMG 135668 BHI, Salmonella enterica, Salmonella typhi, and Neisseria gonorrhea. Our results suggest that Pachylobus balsamifera extracts contain antioxidant, anti-inflammatory, and antimicrobial properties.

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Antimicrobial, antioxidant activities and toxicity on Cavia porcellus of Dialium angolense Welw. Ex Oliv, a traditional medicinal plant from Bagira in Eastern of DR Congo

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2020.13.2.0352 ◽

2020 ◽

Vol 13 (2) ◽

pp. 166-180

Author(s):

Bashige Chiribagula V ◽

Bakari Amuri S ◽

Okusa Ndjolo Philippe ◽

Kahumba Byanga J ◽

Duez P ◽

...

Keyword(s):

Antioxidant Activity ◽

Acute Toxicity ◽

Antioxidant Activities ◽

Antimicrobial Properties ◽

Salmonella Typhi ◽

Antimicrobial Activities ◽

Cavia Porcellus ◽

E Coli

Dialium angolense is used in Bagira for its various medicinal properties particularly in the management of infectious diseases. In this study, the methanol and aqueous extracts of leaves and fruits were evaluated for their in vitro antioxidant and antimicrobial properties and their in vivo toxicity on Cavia porcellus. The major phytochemical classes of extracts were screened using standard in-tube reactions. The antimicrobial study was tested on Candida albicans, Escherichia coli, Salmonella typhi, Staphylococcus aureus and Streptococcus pneumoniae using agar well diffusion and dilution methods, while the antioxidant activity was evaluated by a DPPH assay. For the acute toxicity study, animals (6/group) were orally given in a single dose 5000, 1000 or 15000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assays, 150 or 300 mg/kg BW/day were orally given, and animals observed for 28 days. Total phenolics and total flavonoids contents ranged 1.19 to 1.61 mg GAE.g-1 and 0.45 to 1.01 mg QEg-1, respectively. The extracts presented antioxidant activity with IC50 ranging 4.9 to 6.9 µg/mL. The minimal inhibitory concentration (MIC) on tested strains ranged from 1.9 to 500 µg/mL with the aqueous extract of fruits as a most active extract: MIC=1.9 µg/mL on E. coli and C. albicans. No signs of toxicity were noted during the acute and sub-acute toxicity assessments, suggesting a maximal tolerate doses (MDT) and LD50 > 15000 mg/kg BW. This study highlights the antioxidant and antimicrobial activities of Dialium angolense and suggests that further studies be directed towards the isolation of active compounds.

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Comparative Study on Phenolic Compounds and Antioxidant Activities of Hop (Humulus lupulus L.) Strobile Extracts

Plants ◽

10.3390/plants11010135 ◽

2022 ◽

Vol 11 (1) ◽

pp. 135

Author(s):

Jae Il Lyu ◽

Jaihyunk Ryu ◽

Kyoung-Sun Seo ◽

Kyung-Yun Kang ◽

Sang Hoon Park ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Phenolic Compound ◽

Antioxidant Activities ◽

Water Extract ◽

Ethanol Extract ◽

Antioxidant Property ◽

Flavonoid Compound ◽

Total Phenolic ◽

Extraction Solvent

In this study, we investigated the phenolic compounds in hop strobile extracts and evaluated their antioxidant property using DPPH and ABTS assay. The total phenolic compound (TPC) and total flavonoid compound (TFC) estimated in two different solvent extracts considerably varied depending on the extraction solvent. The most abundant phenolic compound in hop strobile was humulones (α-acid) with levels ranging from 50.44 to 193.25 µg/g. El Dorado accession revealed higher antioxidant activity in ethanol extracts (DPPH: IC50 124.3 µg/mL; ABTS: IC50 95.4 µg/mL) when compared with that of the other accessions. Correlations between DPPH (IC50) scavenging TFC in ethanol extract (TFC_E, −0.941), and TPC_E (−0.901), and between ABTS (IC50) scavenging TFC_E (−0.853), and TPC_E (−0.826), were statistically significant at p < 0.01 level, whereas no significant correlation was observed between antioxidant activities, TPC and TFC in water extract. This study is the first to report that variations in the level of phenolic contents and antioxidant activity of various hop cultivars depended on the type of extraction solvent used and the cultivation regions. These results could provide valuable information on developing hop products.

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Polyphenolic Content, Antioxidant and Antimicrobial Activities of Vernonia mespilifolia Less. Used in Folk Medicine in the Eastern Cape Province, South Africa

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x18773990 ◽

2018 ◽

Vol 23 ◽

pp. 2515690X1877399 ◽

Cited By ~ 9

Author(s):

Jeremiah Oshiomame Unuofin ◽

Gloria Aderonke Otunola ◽

Anthony Jide Afolayan

Keyword(s):

Antioxidant Activities ◽

Folk Medicine ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

In Vitro Assays ◽

Eastern Cape ◽

Polyphenolic Content ◽

Ethanol Extracts ◽

Bacteria And Fungi

Vernonia mespilifolia Less. is a shrub of the Asteraceae family used in the South African traditional medicine system for the management of weight loss, hypertension, and heartwater disease. There is a need for scientific evaluation to validate its ethnomedicinal usage. In vitro assays were conducted to evaluate the polyphenolic content, antioxidant and antimicrobial properties of different solvent extracts (acetone, aqueous, and ethanol) of the whole plant of Vernonia mespilifolia spectrophotometric and agar dilution techniques, respectively. The result revealed varying amounts of polyphenolics in the different solvent extracts corresponding to the antioxidant activities. Also, only the acetone and ethanol extracts inhibited the growth of the selected bacteria and fungi. These findings reveal that the extracts have strong bioactive compounds and hence support its ethnomedicinal application.

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Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities

Zeitschrift für Naturforschung C ◽

10.1515/znc-2020-0257 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Gamze Göger ◽

Muhammed Allak ◽

Ali Şen ◽

Fatih Göger ◽

Mehmet Tekin ◽

...

Keyword(s):

Fatty Acids ◽

Essential Oil ◽

Antioxidant Activities ◽

Antimicrobial Activities ◽

Antidiabetic Activity ◽

Gram Negative Bacteria ◽

Inhibitory Effects ◽

Dicaffeoylquinic Acid ◽

Anti Inflammatory ◽

Phytochemical Profiles

Abstract Phytochemical profiles of essential oil (EO), fatty acids, and n-hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of Cota altissima L. J. Gay (syn. Anthemis altissima L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and β-caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and α-glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. CAM showed the highest antioxidant activity against DPPH and ABTS radicals with IC50 values of 126.60 and 144.40 μg/mL, respectively. In the anti-inflammatory activity, CAE demonstrated the highest antilipoxygenase activity with an IC50 value of 105.40 μg/mL, whereas, CAD showed the best inhibition of α-glucosidase with an IC50 value of 396.40 μg/mL in the antidiabetic activity. CAH was effective against Staphylococcus aureus at MIC = 312.5 µg/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of C. altissima.

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Oriental Hornet (Vespa orientalis) Larval Extracts Induce Antiproliferative, Antioxidant, Anti-Inflammatory, and Anti-Migratory Effects on MCF7 Cells

Molecules ◽

10.3390/molecules26113303 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3303

Author(s):

Amina M. G. Zedan ◽

Mohamed I. Sakran ◽

Omar Bahattab ◽

Yousef M. Hawsawi ◽

Osama Al-Amer ◽

...

Keyword(s):

Phenolic Compounds ◽

Aqueous Extract ◽

Antioxidant Activities ◽

Individual Treatment ◽

Alcoholic Extract ◽

Anticancer Effect ◽

Mcf7 Cells ◽

Vespa Orientalis ◽

Anti Inflammatory

The use of insects as a feasible and useful natural product resource is a novel and promising option in alternative medicine. Several components from insects and their larvae have been found to inhibit molecular pathways in different stages of cancer. This study aimed to analyze the effect of aqueous and alcoholic extracts of Vespa orientalis larvae on breast cancer MCF7 cells and investigate the underlying mechanisms. Our results showed that individual treatment with 5% aqueous or alcoholic larval extract inhibited MCF7 proliferation but had no cytotoxic effect on normal Vero cells. The anticancer effect was mediated through (1) induction of apoptosis, as indicated by increased expression of apoptotic genes (Bax, caspase3, and p53) and decreased expression of the anti-apoptotic gene Bcl2; (2) suppression of intracellular reactive oxygen species; (3) elevation of antioxidant enzymes (CAT, SOD, and GPx) and upregulation of the antioxidant regulator Nrf2 and its downstream target HO-1; (4) inhibition of migration as revealed by in vitro wound healing assay and downregulation of the migration-related gene MMP9 and upregulation of the anti-migratory gene TIMP1; and (5) downregulation of inflammation-related genes (NFκB and IL8). The aqueous extract exhibited the best anticancer effect with higher antioxidant activities but lower anti-inflammatory properties than the alcoholic extract. HPLC analysis revealed the presence of several flavonoids and phenolic compounds with highest concentrations for resveratrol and naringenin in aqueous extract and rosmarinic acid in alcoholic extract. This is the first report to explain the intracellular pathway by which flavonoids and phenolic compounds-rich extracts of Vespa orientalis larvae could induce MCF7 cell viability loss through the initiation of apoptosis, activation of antioxidants, and inhibition of migration and inflammation. Therefore, these extracts could be used as adjuvants for anticancer drugs and as antioxidant and anti-inflammatory agents.

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Antioxidant activities and A549 lung adenocarcinomic cells against capacity from various extracts of Agrimonia pilosa Ledeb

Journal of Ayurvedic and Herbal Medicine ◽

10.31254/jahm.2018.4304 ◽

2018 ◽

Vol 4 (3) ◽

pp. 114-119

Author(s):

Quang Ung Le ◽

◽

Horng Liang Lay ◽

Ming Chang Wu ◽

◽

...

Keyword(s):

Phenolic Compounds ◽

High Performance ◽

Antioxidant Activities ◽

Radical Scavenging ◽

A549 Cells ◽

Total Phenolic ◽

Root Extract ◽

Inhibitory Capacity ◽

Ethanol Extracts

Agrimonia pilosa Ledeb (AL) has received considerable attention as a herbal medicine for its applications in ethnopharmacology with heath benefits. This study aimed to investigate antioxidant activities and A549 growth inhibitory capacity from its root extract (RE) and aerial parts extract (AE). The 50% ethanol extracts were used for the tests. The total polyphenol content and the antioxidant effects comprising ABTS+ and DPPH free radical scavenging activities were evaluated. Phenolic compounds in the extracts were isolated using high performance liquid chromatography (HPLC). Lactate dehydrogenase released in medium was also evaluated. Total phenolic and flavonoid content, and in vitro antioxidant potential of the RE were higher (p<0.01) than that of the AE. Two individual phenolic compounds consisting of 4-hydroxybenzoic acid and p-coumaric acid were firstly identified in both by HPLC. The RE exhibited higher A549 inhibitory capacity compared to the AE and activated the apoptotic proteins of bcl-2, bax, bad, caspase-3 and caspase-9 in A549. In conclusion, the AL extracts were more effective in antioxidant and A549 cells inhibitory capacity.

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Alteration in Phenolic Compounds and Antioxidant Activities of Aronia melanocarpa Ethanol Extracts following Fermentation Using Different Strains of Leuconostoc mesenteroides to Develop Natural Antibiotic Alternative

Korean Journal of Organic Agricultue ◽

10.11625/kjoa.2014.22.4.825 ◽

2014 ◽

Vol 22 (4) ◽

pp. 825-839 ◽

Cited By ~ 1

Author(s):

Joo Hwan Hwang ◽

◽

Ju Hui Kang ◽

Ki Hwan Lee ◽

Jae Hoon Lee ◽

...

Keyword(s):

Phenolic Compounds ◽

Leuconostoc Mesenteroides ◽

Antioxidant Activities ◽

Aronia Melanocarpa ◽

Ethanol Extracts ◽

Different Strains

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Comparison of Antioxidant and Antimicrobial Properties for Ginkgo biloba and Rosemary (Rosmarinus officinalis L.) from Egypt

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4118928 ◽

2013 ◽

Vol 41 (1) ◽

pp. 126 ◽

Cited By ~ 8

Author(s):

Hossam S. EL-BELTAGI ◽

Mona H. BADAWI

Keyword(s):

Phenolic Compounds ◽

Ginkgo Biloba ◽

Metal Ion ◽

Antioxidant Activities ◽

Antimicrobial Activities ◽

Rosmarinus Officinalis ◽

Carnosic Acid ◽

Diffusion Method ◽

Total Phenolic ◽

Agar Disc

The widespread use of medicinal plants for health purposes has increased dramatically due to their great importance to the public health. In this study levels of phenolic, flavonoid contents of Ginkgo biloba and Rosmarinus officinalis from Egypt were determined. HPLC was used to identify and quantify the phenolic compounds in selected plants. The plant extracts were evaluated for their antioxidant activities using various antioxidant methodologies, (i) scavenging of free radicals using 2, 2-diphenyl-1-picrylhydrazyl, (ii) metal ion chelating capacity, and (iii) scavenging of superoxide anion radical. The antimicrobial activity of both plantâ€™s extracts were evaluated against a panel of microorganisms by using agar disc diffusion method. The total phenolic content (75.30 and 98.31 mg/g dry weight in G. biloba and R. officinalis, respectively) was significantly (p<0.05) different. Among the identified phenolic compounds, quercetin, kaempferol and caffeic acid were the predominant phenolic compounds in Ginkgo biloba, whereas carnosic acid, rosmarinic acid, narinigen and hispidulin were the predominant phenolic compound in Rosmarinus officinalis leaves. The antioxidant activity increased with the concentration increase. The R. officinalis was more active than G. biloba extract against Gram-negative bacteria. This study reveals that the consumption of these plants would exert several beneficial effects by virtue of their antioxidant and antimicrobial activities.

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