Circulating Serum Amyloid A, hs-CRP and Vitamin D Levels in Postmenopausal Osteoporosis

Background: Both vitamin D and inflammation were investigated as important players in the pathogenesis of postmenopausal osteoporosis. This study compared vitamin D, inflammatory the biomarkers serum levels and their association with bone mineral density (BMD) in case and control groups to evaluate the possible immune-regulatory effect of vitamin D in this population. Materials and Methods: Participants in post-menopausal age, were categorized to 44 osteoporotic vs. 44 healthy aged-matched women according to WHO criteria. Total BMD, T- scores, Z-scores as well as fracture risk were measured in both groups, using Hologic system Dual-energy X-ray absorptiometry (DEXA). Serum 25-OH vitamin D, high sensitive CRP (hs-CRP) and serum amyloid A (SAA) were compared between groups. The association between serum biomarkers level and BMD were also investigated. The same evaluations were performed for vitamin D deficient (<20 ng/mL) and non-deficient (≥20 ng/mL) subgroups. Results: Vitamin D deficiency was higher in the osteoporotic group (32.6%) in comparison with the control group (25.6%), but the differences were not significant (P=0.47). There were no significant differences in serum levels of hs-CRP and SAA (P=0.83 and P=0.39) as well. No significant association between serum inflammatory biomarkers, vitamin D, and BMD were detected (P≥0.05). The results were the same for vitamin D deficient and non-deficient subgroups (P≥0.05). Conclusion: In the current study, the beneficial effects of vitamin D as a result of its immune-regulatory mechanisms was not reached. Larger scale studies might pave the way to define vitamin D benefits in postmenopausal osteoporosis. [GMJ.2019;8:e1548]

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Vitamin D status, bone mineral density and body mass index in patients with newly diagnosed postmenopausal osteoporosis and healthy menopausal women

10.35465/27.3.2019.pp18-28 ◽

2019 ◽

pp. 18-28

Keyword(s):

Vitamin D ◽

Alkaline Phosphatase ◽

Postmenopausal Osteoporosis ◽

Control Group ◽

Winter Period ◽

Mineral Density ◽

Vitamin D Levels ◽

Menopausal Women ◽

Calcium Phosphorus ◽

D 12

This study aims to assess vitamin D levels in women with postmenopausal osteoporosis and healthy women going through menopause. Two groups of menopausal women took part in the study – 41 women with osteoporosis and 22 without osteoporosis. The levels of vitamin D, parathormone, alkaline phosphatase, calcium and phosphorus were examined during the autumn-winter period. Calcium, phosphorus and alkaline phosphatase were established within the reference range in both groups. A negative correlation with increase of parathormone levels among the patients with osteoporosis and vitamin D deficiency was found (r = -0.46, p<0.01). 16 women with osteoporosis (39%) were with normal levels of vitamin D, 14 (34,1%) were with insufficiency and 11 (26,9%) with deficiency. Within the control group, 8 women (36,4%) were with normal levels of vitamin D, 12 (54,5%) were with insufficiency and 2 (9,1%) with deficiency.

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Association of serum amyloid A levels with adipocyte size and serum levels of adipokines: Differences between men and women

Cytokine ◽

10.1016/j.cyto.2009.08.005 ◽

2009 ◽

Vol 48 (3) ◽

pp. 260-266 ◽

Cited By ~ 13

Author(s):

Kajsa Sjöholm ◽

Magdalena Lundgren ◽

Maja Olsson ◽

Jan W. Eriksson

Keyword(s):

Serum Amyloid A ◽

Serum Levels ◽

Serum Amyloid ◽

Adipocyte Size ◽

Men And Women ◽

Amyloid A

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The Correlations Between Concentrations of Myeloperoxidase, Serum Amyloid-A Protein and Scretory Phospolipase A-2 with Proinflammatory HDL in Healthy Male Person

The Indonesian Biomedical Journal ◽

10.18585/inabj.v1i1.83 ◽

2009 ◽

Vol 1 (1) ◽

pp. 53

Author(s):

Marita Kaniawati ◽

Andi Wijaya ◽

Anwar Susanto

Keyword(s):

Serum Amyloid A ◽

Independent Predictor ◽

Multiple Logistic Regression Analysis ◽

Serum Amyloid ◽

Multiple Logistic Regression ◽

Amyloid A ◽

Inflammatory Status ◽

Serum Amyloid A Protein ◽

Low Hdl ◽

Hs Crp

BACKGROUND: Low-HDL cholesterol is a risk factor of CAD. Although levels of HDLC are within normal limit in some patients, they suffer CAD. These normal HDL-C levels might become pro-inflammatoric. This study is to measure the correlations between myeloperoxidase (MPO), serum amyloid-A (SAA) protein, and secretoryphospholipase-A2 (sPLA2) with inflammatory status of HDL-C.METHODS: This was a cross-sectional study recruited 49 subjects with high HDL-C (> 40 mg/dL) and 31 subjects with low HDL-C (< 40 mg/dL). HDL-C was determined into antiinflammatory and proinflammatory based on levels of Apo A-1 and hs-CRP. Concentrations of MPO, SAA and s-PLA2 were measured by ELISA method. Levels of Apo A-1 was determined by immunoturbidimetric method. Multiple logistic regression analysis was done using inflammatory status of HDL-C as dependent variables and levels of MPO, SAA, sPLA2, ages, total cholesterol and triglycerides as independent variables.RESULTS: Patient’s age was 43.4 + 8.3 year, HDL-C was 43.1 + 9.5 mg/dL, Apo A-1 was 128.3 + 21.5 mg/dL, hs-CRP was 1.92 + 3.0 mg/dL. Concentrations of MPO, SAA and sPLA2 successively were 63.2 + 16.9 ng/mL, 7015.6 + 5021.1 ng/mL and 1340.2 + 406.3 pg/mL. Multiple logistic regression analysis showed that SAA is an independent predictor of pro-inflammatory status of HDL-C in high HDL-C group with prevalence ratio of 11.74 (95% CI : 2.51 – 54.84; P = 0.002). In contrast, MPO and sPLA2 were not independent predictor with PR of 1.26 (95% CI : 0.30 – 5.23; P = 0.75) and of 0.94 (95% CI : 0.23 – 3.91; P = 0.93).CONCLUSIONS: SAA is an independent predictor of pro-inflammatory HDL-C even in subjects with high HDL-C.KEYWORDS: Atherosclerosis, Apo A-I, serum amyloid A protein, secretory phospholipase A2, myeloperoxidase

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Serum Amyloid a Protein and C-Reactive Protein — a Comparison of Serum Levels in Cancer and Non-Malignant Disease

Protides of the Biological Fluids ◽

10.1016/b978-0-08-030764-0.50085-9 ◽

1984 ◽

pp. 375-378

Author(s):

J.G. RAYNES ◽

E.H. COOPER

Keyword(s):

Malignant Disease ◽

Serum Amyloid A ◽

Protein A ◽

Serum Levels ◽

Serum Amyloid ◽

C Reactive Protein ◽

Reactive Protein ◽

Amyloid A ◽

Serum Amyloid A Protein

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Physiotherapy protocol during initial postoperative period of arthroscopy in horses

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5655 ◽

2018 ◽

Vol 38 (12) ◽

pp. 2201-2206

Author(s):

Fernanda C. Stievani ◽

Thais S.L. Machado ◽

Kaio B. Bezerra ◽

Marilene M. Silva ◽

Raquel Y.A. Baccarin ◽

...

Keyword(s):

Synovial Fluid ◽

Physical Examination ◽

Postoperative Period ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Control Group ◽

Physical Analysis ◽

Amyloid A ◽

Significant Difference ◽

Significant Change

ABSTRACT: This study evaluated the effects of a physiotherapy protocol applied in joints with osteochondritis dissecans submitted to arthroscopy. Twelve horses totaling twenty joints were used and divided into two uniform groups, according to articular lesion grade. Treated Group (TG) received the physiotherapy protocol (cryotherapy, passive rage motion and controlled exercise) that initiate just after anesthetic recovery and extended for five days. Control Group (CG) remained resting in stall during the same period. Physical examination and synovial fluid analysis were used to evaluate the treatment. The synovial fluid examination consisted of physical analysis (color, aspect, and viscosity), mucin clot evaluation, Serum Amyloid A, Prostaglandin E2 and urea concentration. Synovial samples were collected by arthrocentesis at the beginning of the surgical procedure (D1), 48 hours (D3) and 96 hours (D5) after surgery. Before arthroscopy and daily during the postoperative period joints were evaluated by physical exam: superficial temperature (°C), range of motion (degrees) and circumference (centimeters). The joint physical examination showed no significant difference between groups and neither along the days for the same group. The parameters of synovial fluid showed difference over the moments in each group but didn’t have difference between groups. Color and aspect had the same patterns across moments, in CG fluid had significant change when compared D1 with D3 (color and aspect: p<0.001) and D5 (color: p<0.001; aspect: p<0.05) becoming mostly bloody and cloudy in D3 and D5. However in TG the difference was significant just between D1 and D3 (color and aspect: p<0.05), showing an improvement of synovial fluid in D5 (color and aspect: p>0.05). Viscosity and mucin clot evaluation showed significant change in CG between D1 and D3 (viscosity: p<0.01; mucin clot: p<0.05) and between D1 and D5 (viscosity: p<0.01;mucin clot: p<0.01). In TG no significant difference of viscosity and mucin clot was observed over the moments, showing an early improvement of synovial fluid quality. The Serum Amyloid A concentration showed an extremely significant increase in CG (p<0.001) when compared D1 (1217.13±664.47μg/mL) and D3 (42423.80±52309.31μg/mL). The comparison between D1 and D5 in CG, and across moments in TG, had no statistical difference. The PGE2 eicosanoid remained statistically unchanged all over the time. Urea showed significant increase in D3 when compared to D1 (p<0.001) in CG, and had no variation in TG. The physiotherapy protocol minimized the inflammatory mediators and provided minor alterations in synovial fluid after arthroscopy.

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Detection of serum amyloid-A concentration in the calf clinically diagnosed with pneumonia, enteritis and pneumoenteritis

Ciência Rural ◽

10.1590/0103-8478cr20150571 ◽

2016 ◽

Vol 46 (2) ◽

pp. 293-299 ◽

Cited By ~ 1

Author(s):

Mustafa Kabu ◽

Bulent Elitok ◽

Ismail Kucukkurt

Keyword(s):

Veterinary Medicine ◽

Blood Cell ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Control Group ◽

Amyloid A ◽

Tnf Α ◽

Concentration Measurements ◽

Severity Of The Disease ◽

Commercial Kits

ABSTRACT: The aim of this study is to determine serum amyloid-A (SAA) concentration in the cases of pneumonia, pneumoenteritis, and enteritis which are frequently encountered in calves in veterinary medicine. Although a great deal of experimental studies has been conducted in this field, studies on naturally infected calves are quite few. Eighty calves at the age of 0-6 months were used in the study and the calves were divided into four groups. Due to the clinical examination, the calves diagnosed with pneumonia (Group P; n=20), with pneumoenteritis (Group PE; n=20) and with enteritis (Group E; n=20) formed the disease group as the healthy ones formed the control (Group C; n=20) group. After the body temperatures of all calves were taken, blood samples were obtained from Jugular vein for haematological and biochemical measurements. As haematological, white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) measurements were performed in Veterinary Hematology Analyzer. Serum amyloid-A (SAA), interleukin 1 (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) concentration measurements were carried out with ELISA reader by using commercial kits. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin (ALB), total bilirubin (T. Bil), total protein (TP), gamma glutamyltransferase (GGT), blood urea nitrogen (BUN) concentration measurements were conducted in autoanalyzer by using commercial kits. In all disease groups (P, PE, and E) body temperature, haematologic parameters (WBC, RBC, Hb and Hct), serum biochemical parameters (AST, ALT, ALB, T. Bil, TP, GGT and BUN), SAA concentration and serum concentrations of cytokines (IL-1β, IL-6 and TNF-α) were determined to be higher in comparison to the control group (P<0.005). According to these findings, routine measurement of serum SAA concentration in veterinary medicine is considered to be beneficial in determining the severity of the disease, in selecting the proper treatment, in monitoring the applied treatment, and detecting subclinical diseases. In the light of these findings we acknowledge that routine measurements of serum SAA concentration from the moment the calves are diagnosed with pneumonia, enteritis and pneumoenteritis in veterinary medicine until the actual cause is determined (bacteria, virus, parasites, etc.) would avail the clinician to, identify the severity of the disease, select the appropriate treatment and monitor the effectiveness of the treatment.

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Are Vitamin D Levels Associated With Risk of Deep Neck Infection?

Ear Nose & Throat Journal ◽

10.1177/0145561319865498 ◽

2019 ◽

pp. 014556131986549

Author(s):

Mustafa Sıtkı Gozeler ◽

Muhammed Sedat Sakat ◽

Korhan Kilic ◽

Abdulkadir Sahin ◽

Arzu Tatar ◽

...

Keyword(s):

Vitamin D ◽

Immune System ◽

Vitamin D Deficiency ◽

Serum Levels ◽

Control Group ◽

Deep Neck Infection ◽

Neck Infection ◽

Vitamin D Receptors ◽

Vitamin D Levels ◽

25 Hydroxy Vitamin D

Deep neck infection (DNI) refers to infections in spaces created by superficial and deep cervical fascia around the muscles and organs in the neck. Vitamin D is highly important for an effective immune system. Vitamin D receptors (VDR) have been identified in immune system cells, and particularly in T and B lymphocytes, macrophages, and dendritic cells. Vitamin D deficiency is thought to result in impaired immune response, decreased leukocyte chemotaxis, and an increased disposition to infection. The purpose of this study was to investigate whether vitamin D deficiency is an underlying occult factor in the development of DNI. Sixty-five patients aged 6 to 90, diagnosed with DNI, and 70 healthy age- and sex-compatible cases were included in the study. Serum levels of calcium, phosphorus, parathyroid hormone, and 25-hydroxy vitamin D (25(OH)D) were determined in each case. 25-hydroxy vitamin D levels above 20 ng/mL were regarded as normal, 12 to 20 ng/mL as insufficient, 5 to 12 ng/mL as deficient, and less than 5 ng/mL as severely deficient. Mean serum 25(OH)D levels were 10.4 (6.2) ng/mL in the patient group and 15.5 (6.4) ng/mL in the control group ( P < .01). This difference was statistically significant ( P < .01). Vitamin D was within normal limits in 9.2% (n = 6) of cases in the study group, insufficient in 29.2% (n = 19), deficient in 35.3% (n = 23), and severely deficient in 26.2% (n = 17). The equivalent values in the control group were 21.4% (n = 15), 48.5% (n = 34), 30% (n = 21), and 0% (n = 0). Serum 25(OH)D levels were significantly lower in patients with DNI compared to the healthy cases; 25(OH)D levels may be a factor in the development of DNI.

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Can YKL-40 be an Inflammatory Biomarker in Vitamin D Deficiency?

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000545 ◽

2019 ◽

Vol 89 (5-6) ◽

pp. 309-313

Author(s):

Ummugulsum Can ◽

Saliha Uysal ◽

Ayse Ruveyda Ugur ◽

Aysun Toker ◽

Uysaler Aslan ◽

...

Keyword(s):

Vitamin D ◽

Vitamin D Deficiency ◽

Serum Levels ◽

Normal Serum ◽

Plasma Vitamin ◽

Inflammatory Biomarker ◽

Vitamin D Levels ◽

Group 2 ◽

Hs Crp ◽

Group 1

Abstract. Vitamin D deficiency is associated with several non-homeostatic conditions and/or diseases like inflammation, atherosclerosis, cardiovascular disease and mortality. YKL-40 is a glycoprotein, secreted by macrophages, neutrophils and different cell types and it is also associated with inflammation and pathological tissue remodeling. In this study, we aimed to evaluate relationship between the proinflammatory biomarkers YKL-40 and hs-CRP levels and vitamin D deficiency. Our study group includes 45 subjects with vitamin D deficiency (Group 1) (20 M, 25 F; mean age 37.72 ± 7.70 years) and 40 age and sex-matched healthy subjects with normal serum levels of vitamin D (Group 2) (19 M, 21 F; mean age 39.26 ± 7.41 years). Plasma 25 (OH) vitamin D levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Plasma YKL-40 analysis was performed by ELISA. Serum hs-CRP levels were measured by nephelometric method. Plasma vitamin D levels below 20 ng/mL were accepted as vitamin D deficiency. Although we could not find any significant differences by means of serum hs-CRP levels between Group 1 and Group 2 (2.21 (0.27–11.70); 1.79 (0.16–9.85) mg/L, p = 0.247), plasma YKL-40 levels were significantly higher in group 1 than group2 (70.47 (17.84–198.50); 47.14 (4.80–135.48) ng/mL, p = 0.047). In literature, vitamin D deficiency is associated with inflammation. In our study, we found similar hs-CRP levels between groups and higher YKL-40 levels in group 1. Vitamin D deficiency may be related to high YKL-40 levels in terms of causing chronic inflammation.

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Serum Amyloid A Level in Women with Unexplained Infertility

QJM ◽

10.1093/qjmed/hcab115.039 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Mostafa Ibrahim ◽

Hayam Fathy Mohammad ◽

Nashwa Nagy el-Khazragy ◽

Zeinab Saad Abd El wahab

Keyword(s):

Research Group ◽

Inflammatory Process ◽

Serum Amyloid A ◽

Research Study ◽

Maternity Hospital ◽

Unexplained Infertility ◽

Serum Amyloid ◽

Control Group ◽

Chronic Inflammatory Process ◽

Amyloid A

Abstract Background By various molecular and cellular research efforts it was displayed that folliculogenesis, ovulation, and corpus luteum formation is considered as an inflammatory processes that hav raised the research interest to investigate that serum amyloid A as an inflammatory mediator to reveal its possible role in infertility. Objective This study aims to assess the association between SAA and unexplained infertility in women. Methods The current research study have been conducted at Ain Shams University Maternity Hospital during the period from September 2018 to April 2019. Women approached were recruited from outpatient clinic of Ain Shams University Maternity Hospital. The recruited 90 research study subjects have been divided into two research groups Results Serum amyloid A was statistically significantly higher among the unexplained research group versus control group (SAA had excellent discriminative value with an area under the ROC curve (AUC) of 0.992 (95% CI = 0.945 to 1.000, Pvalue <0.0001). A best cutoff criterion is SAA of 28.7 mg/l (sensitivity = 98%, specificity = 96%, J-index = 0.93)) denoting that amyloid A as an acute phase inflammatory modulator is higher among the unexplained research group reflecting a form of chronic inflammatory process in those category of cases. Analytical research results of multivariable binary logistic regression analysis as regards the correlation between SAA and unexplained infertility after adjustment for age and BMI, it was revealed that serum amyloid A an independent predictor for unexplained infertility. Conclusion s: Serum amyloid A as a biomarker for could be implemented as a predictability tool for unexplained infertility since it is a reflector of an chronic inflammatory process that is ongoing that could affect the process of implantation and ovulation however the clear molecular and cellular processes linked to the pathophysiological process that reflects the fertility hindering process should be implemented in future research efforts taking in consideration to have multicentric fashion of research with larger sample sizes to verify the value of serum amyloid A as biomarker that reflects the fertility potential besides the routine infertility work up.

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