Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Background: 3,4-Methylenedioxymethamphetamine is psychoactive and hallucinogenic and has been shown to produce neurotoxicity both in animals and in humans. Recently, vasodilator drugs such as pentoxifylline (PTX) have been introduced as an alternative with neuroprotective effects. There is no study about the protective effect of PTX on hippocampal apoptosis due to high-dose administration of 3,4-Methylenedioxymethamphetamine (MDMA), so in this study, the protective effect of PTX on the hippocampus of male Wistar rats following high-dose of the drug has been investigated. Materials and Methods: Twenty-four male Wistar rats weighing 250-300 g were randomly divided into four groups: control, sham (MDMA injection), experimental (MDMA+PTX injection), and vehicle (MDMA+saline) groups. Two weeks later, the brains were removed and prepared for TUNEL and western blot techniques. Concomitantly the hippocampus was removed to study the change in Bcl-2 and BAX mRNA expression with quantitative real-time polymerase chain reaction. Results: Data showed that the number of apoptotic bodies significantly decreased in the experimental group compared to the other groups, except for in control. Also, further investigation revealed that BAX reduced considerably, while Bcl-2 mRNA expression increased dramatically after PTX treatment. Conclusions: Our results suggest that PTX may be a neuroprotective agent, and its neuroprotective potential may contribute to reducing the severity of lesions in the hippocampus following a high dose administration of MDMA. [GMJ.2019;8:e963]

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d-Galactose High-Dose Administration Failed to Induce Accelerated Aging Changes in Neurogenesis, Anxiety, and Spatial Memory on Young Male Wistar Rats

Rejuvenation Research ◽

10.1089/rej.2015.1684 ◽

2015 ◽

Vol 18 (6) ◽

pp. 497-507 ◽

Cited By ~ 17

Author(s):

Armando Cardoso ◽

Sara Magano ◽

Francisco Marrana ◽

José P. Andrade

Keyword(s):

Spatial Memory ◽

Wistar Rats ◽

Accelerated Aging ◽

Young Male ◽

High Dose ◽

Dose Administration ◽

Male Wistar Rats

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Possible Synergistic Antidiabetic Effects of Quantified Artemisia judaica Extract and Glyburide in Streptozotocin-Induced Diabetic Rats via Restoration of PPAR-α mRNA Expression

Biology ◽

10.3390/biology10080796 ◽

2021 ◽

Vol 10 (8) ◽

pp. 796

Author(s):

Abdulaziz S. Saeedan ◽

Gamal A. Soliman ◽

Rehab F. Abdel-Rahman ◽

Reham M. Abd-Elsalam ◽

Hanan A. Ogaly ◽

...

Keyword(s):

Lipid Profile ◽

Mrna Expression ◽

Wistar Rats ◽

Diabetic Rats ◽

High Dose ◽

Oral Antidiabetic Drugs ◽

Insulin Levels ◽

Oral Antidiabetic ◽

Male Wistar Rats ◽

Dose Combination

Several members of the genus Artemisia are used in both Western and African traditional medicine for the control of diabetes. A considerable number of diabetic patients switch to using oral antidiabetic drugs in combination with certain herbs instead of using oral antidiabetic drugs alone. This study examined the effect of Artemisia judaica extract (AJE) on the antidiabetic activity of glyburide (GLB) in streptozotocin (STZ)-induced diabetes. Forty-two male Wistar rats were divided into seven equal groups. Normal rats of the first group were treated with the vehicle. The diabetic rats in the second–fifth groups received vehicle, GLB (5 mg/kg), AJE low dose (250 mg/kg), and AJE high dose (500 mg/kg), respectively. Groups sixth–seventh were treated with combinations of GLB plus the lower dose of AJE and GLB plus the higher dose of AJE, respectively. All administrations were done orally for eight weeks. Fasting blood glucose (FBG) and insulin levels, glycated hemoglobin (HbA1c) percentage, serum lipid profile, and biomarkers of oxidative stress were estimated. The histopathological examination of the pancreas and the immunohistochemical analysis of anti-insulin, anti-glucagon, and anti-somatostatin protein expressions were also performed. The analysis of the hepatic mRNA expression of PPAR-α and Nrf2 genes were performed using quantitative RT-PCR. All treatments significantly lowered FBG levels when compared with the STZ-control group with the highest percentage reduction exhibited by the GLB plus AJE high dose combination. This combination highly improved insulin levels, HbA1c, and lipid profile in blood of diabetic rats compared to GLB monotherapy. In addition, all medicaments restored insulin content in the β-cells and diminished the levels of glucagon and somatostatin of the α- and δ-endocrine cells in the pancreatic islets. Furthermore, the GLB plus AJE high dose combination was the most successful in restoring PPAR-α and Nrf2 mRNA expression in the liver. In conclusion, these data indicate that the GLB plus AJE high dose combination gives greater glycemic improvement in male Wistar rats than GLB monotherapy.

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Protective effect of vitamin C against ivermectin induced nephrotoxicity in different age groups of male wistar rats: bio-histopathological study

Anatomy & Cell Biology ◽

10.5115/acb.21.124 ◽

2021 ◽

Author(s):

Shereen E. Tawfeek ◽

Ayat M. Domouky ◽

Reham H. Abdel-Kareem

Keyword(s):

Vitamin C ◽

Protective Effect ◽

Wistar Rats ◽

Age Groups ◽

Histopathological Study ◽

Male Wistar Rats

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Protective effect of ethanolic extract of seeds of Coriandrum sativum l. in acetic acid-induced ulcerative colitis in male wistar rats

Journal of Integrated Health Sciences ◽

10.4103/2347-6486.239502 ◽

2013 ◽

Vol 1 (2) ◽

pp. 95 ◽

Cited By ~ 1

Author(s):

M Mohan ◽

S Poojari ◽

R Bhargavi

Keyword(s):

Ulcerative Colitis ◽

Acetic Acid ◽

Protective Effect ◽

Wistar Rats ◽

Ethanolic Extract ◽

Coriandrum Sativum ◽

Male Wistar Rats

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Differences in the Role of HDACs 4 and 5 in the Modulation of Processes Regulating MAFbx and MuRF1 Expression during Muscle Unloading

International Journal of Molecular Sciences ◽

10.3390/ijms21134815 ◽

2020 ◽

Vol 21 (13) ◽

pp. 4815 ◽

Cited By ~ 1

Author(s):

Ekaterina P. Mochalova ◽

Svetlana P. Belova ◽

Tatiana Y. Kostrominova ◽

Boris S. Shenkman ◽

Tatiana L. Nemirovskaya

Keyword(s):

Skeletal Muscle ◽

Mrna Expression ◽

Histone Deacetylases ◽

Wistar Rats ◽

Hindlimb Suspension ◽

Skeletal Muscle Atrophy ◽

E3 Ligases ◽

Male Wistar Rats ◽

Muscle Unloading

Unloading leads to skeletal muscle atrophy via the upregulation of MuRF-1 and MAFbx E3-ligases expression. Reportedly, histone deacetylases (HDACs) 4 and 5 may regulate the expression of MuRF1 and MAFbx. To examine the HDAC-dependent mechanisms involved in the control of E3-ubiquitin ligases expression at the early stages of muscle unloading we used HDACs 4 and 5 inhibitor LMK-235 and HDAC 4 inhibitor Tasqinimod (Tq). Male Wistar rats were divided into four groups (eight rats per group): nontreated control (C), three days of unloading/hindlimb suspension (HS) and three days HS with HDACs inhibitor LMK-235 (HSLMK) or Tq (HSTq). Treatment with LMK-235 diminished unloading-induced of MAFbx, myogenin (MYOG), ubiquitin and calpain-1 mRNA expression (p < 0.05). Tq administration had no effect on the expression of E3-ligases. The mRNA expression of MuRF1 and MAFbx was significantly increased in both HS and HSTq groups (1.5 and 4.0 folds, respectively; p < 0.05) when compared with the C group. It is concluded that during three days of muscle unloading: (1) the HDACs 4 and 5 participate in the regulation of MAFbx expression as well as the expression of MYOG, ubiquitin and calpain-1; (2) the inhibition of HDAC 4 has no effect on MAFbx expression. Therefore, HDAC 5 is perhaps more important for the regulation of MAFbx expression than HDAC 4.

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Protective effect of Abutilon indicum against lead‐induced reproductive toxicity in male Wistar rats

Journal of Cellular Biochemistry ◽

10.1002/jcb.28395 ◽

2019 ◽

Vol 120 (7) ◽

pp. 11196-11205 ◽

Cited By ~ 3

Author(s):

Santhoshkumar R. ◽

Asha Devi S.

Keyword(s):

Protective Effect ◽

Wistar Rats ◽

Reproductive Toxicity ◽

Male Wistar Rats ◽

Abutilon Indicum

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Protective effect of antioxidant rich aqueous curry leaf ( Murraya koenigii ) extract against gastro-toxic effects of piroxicam in male Wistar rats

Toxicology Reports ◽

10.1016/j.toxrep.2014.06.007 ◽

2014 ◽

Vol 1 ◽

pp. 987-1003 ◽

Cited By ~ 7

Author(s):

Syed Benazir Firdaus ◽

Debosree Ghosh ◽

Aindrila Chattyopadhyay ◽

Mousumi Dutta ◽

Sudeshna Paul ◽

...

Keyword(s):

Protective Effect ◽

Wistar Rats ◽

Toxic Effects ◽

Murraya Koenigii ◽

Male Wistar Rats

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Effect of Heweianshen Decoction on Orexin-A and Cholecystokinin-8 Expression in Rat Models of Insomnia

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/8034263 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4

Author(s):

Fagen Li ◽

Shaodan Li ◽

Yi Liu ◽

Ke Cao ◽

Minghui Yang

Keyword(s):

Rat Model ◽

Wistar Rats ◽

High Dose ◽

Intragastric Administration ◽

Group Model ◽

Model Group ◽

Orexin A ◽

Blank Group ◽

Rat Models ◽

Male Wistar Rats

Objective. To study the effect of Heweianshen decoction (HAD) on orexin-A and cholecystokinin-8 (CCK-8) expression in rat models of insomnia caused by injecting parachlorophenylalanine (PCPA) intraperitoneally.Methods. Fifty male Wistar rats were randomly divided into five groups (10 rats in each group): blank group, model group, and low-, medium-, and high-dose HAD-treated groups. A rat model of insomnia was established by injecting intraperitoneally with PCPA (300 mg/kg body weight). Rats were given normal saline (10 mL/kg) or 5.25, 10.5, and 21 g/kg HAD by intragastric administration once a day for 6 days. After that, the rats were sacrificed to collect the hypothalamus for tests, using radioimmunoassay to detect the expression of orexin-A and CCK-8.Results. Heweianshen decoction reduced the expression of orexin-A and increased the expression of CCK-8 in the hypothalamus of rat model of insomnia.Conclusion. The therapeutic effect of HAD on insomnia is partially attributed to the decreased expression of orexin-A and increased expression of CCK-8.

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Cerebroprotective Effect ofMoringa oleiferaagainst Focal Ischemic Stroke Induced by Middle Cerebral Artery Occlusion

Oxidative Medicine and Cellular Longevity ◽

10.1155/2013/951415 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 30

Author(s):

Woranan Kirisattayakul ◽

Jintanaporn Wattanathorn ◽

Terdthai Tong-Un ◽

Supaporn Muchimapura ◽

Panakaporn Wannanon ◽

...

Keyword(s):

Oxidative Stress ◽

Ischemic Stroke ◽

Protective Effect ◽

Middle Cerebral Artery ◽

Cerebral Artery ◽

Brain Infarction ◽

Artery Occlusion ◽

High Dose ◽

Infarction Volume ◽

Male Wistar Rats

The protection against ischemic stroke is still required due to the limitation of therapeutic efficacy. Based on the role of oxidative stress in stroke pathophysiology, we determined whetherMoringa oleifera, a plant possessing potent antioxidant activity, protected against brain damage and oxidative stress in animal model of focal stroke.M. oleiferaleaves extract at doses of 100, 200 and 400 mg·kg−1was orally given to male Wistar rats (300–350 g) once daily at a period of 2 weeks before the occlusion of right middle cerebral artery (Rt.MCAO) and 3 weeks after Rt.MCAO. The determinations of neurological score and temperature sensation were performed every 7 days throughout the study period, while the determinations of brain infarction volume, MDA level, and the activities of SOD, CAT, and GSH-Px were performed 24 hr after Rt.MCAO. The results showed that all doses of extract decreased infarction volume in both cortex and subcortex. The protective effect of medium and low doses of extract in all areas occurred mainly via the decreased oxidative stress. The protective effect of the high dose extract in striatum and hippocampus occurred via the same mechanism, whereas other mechanisms might play a crucial role in cortex. The detailed mechanism required further exploration.

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