d-Galactose High-Dose Administration Failed to Induce Accelerated Aging Changes in Neurogenesis, Anxiety, and Spatial Memory on Young Male Wistar Rats

Background: 3,4-Methylenedioxymethamphetamine is psychoactive and hallucinogenic and has been shown to produce neurotoxicity both in animals and in humans. Recently, vasodilator drugs such as pentoxifylline (PTX) have been introduced as an alternative with neuroprotective effects. There is no study about the protective effect of PTX on hippocampal apoptosis due to high-dose administration of 3,4-Methylenedioxymethamphetamine (MDMA), so in this study, the protective effect of PTX on the hippocampus of male Wistar rats following high-dose of the drug has been investigated. Materials and Methods: Twenty-four male Wistar rats weighing 250-300 g were randomly divided into four groups: control, sham (MDMA injection), experimental (MDMA+PTX injection), and vehicle (MDMA+saline) groups. Two weeks later, the brains were removed and prepared for TUNEL and western blot techniques. Concomitantly the hippocampus was removed to study the change in Bcl-2 and BAX mRNA expression with quantitative real-time polymerase chain reaction. Results: Data showed that the number of apoptotic bodies significantly decreased in the experimental group compared to the other groups, except for in control. Also, further investigation revealed that BAX reduced considerably, while Bcl-2 mRNA expression increased dramatically after PTX treatment. Conclusions: Our results suggest that PTX may be a neuroprotective agent, and its neuroprotective potential may contribute to reducing the severity of lesions in the hippocampus following a high dose administration of MDMA. [GMJ.2019;8:e963]

Download Full-text

Vitamin A deficiency regulates the expression of ferritin in young male Wistar rats

Revista de Nutrição ◽

10.1590/1678-9865202134e200297 ◽

2021 ◽

Vol 34 ◽

Author(s):

Mauricio RESTREPO-GALLEGO ◽

Luis Eduardo DÍAZ ◽

Juan David OSPINA-VILLA ◽

Danny CHINCHILLA-CÁRDENAS

Keyword(s):

Iron Deficiency ◽

Vitamin A ◽

Biochemical Parameters ◽

Serum Iron ◽

Wistar Rats ◽

Vitamin A Deficiency ◽

Young Male ◽

Control Group ◽

Transferrin Receptors ◽

Male Wistar Rats

ABSTRACT Objective Iron deficiency and vitamin A deficiency are two of the main micronutrient deficiencies. Both micronutrients are essential for human life and children's development. This study aimed to investigate the effects of vitamin A deficiency on ferritin and transferrin receptors' expression and its relationship with iron deficiency. Methods Five diets with different vitamin A-to-iron ratios were given to thirty five 21-day-old male Wistar rats (separated in groups of seven animals each). The animals received the diet for six weeks before being euthanized. Serum iron and retinol levels were measured as biochemical parameters. Their duodenums, spleens, and livers were analyzed for the expression of ferritin and transferrin receptors by Western Blotting. Results Regarding biochemical parameters, the results show that when both vitamin A and iron are insufficient, the serum iron content (74.74µg/dL) is significantly lower than the control group (255.86µg/dL). The results also show that vitamin A deficiency does not influence the expression of the transferrin receptor, but only of the ferritin one. Conclusion Vitamin A deficiency regulates the expression of ferritin in young male Wistar rats.

Download Full-text

The effect of heat treatment and particle size of bran on mineral absorption in rats

British Journal Of Nutrition ◽

10.1079/bjn19820132 ◽

1982 ◽

Vol 48 (3) ◽

pp. 467-475 ◽

Cited By ~ 14

Author(s):

Andrea Caprez ◽

Susan J. Fairweather-Tait

Keyword(s):

Heat Treatment ◽

Particle Size ◽

Isotope Dilution ◽

Wistar Rats ◽

Specific Radioactivity ◽

Young Male ◽

Breakfast Cereal ◽

Synthetic Diet ◽

Male Wistar Rats ◽

The Mean

1. The effect of heat treatment of bran on true zinc absorption was measured using an isotope-dilution technique. A bran-based breakfast cereal (heated to 204° for 40 min during manufacture) was incorporated into a semi-synthetic diet at a level of 180 g/kg, A parallel diet was formulated containing an identical weight of untreated bran from the same source plus other ingredients used to make the cereal.2. Young male Wistar rats (mean weight 80 g) were injected intramuscularly with 65Zn to label body Zn. They were given the heat-treated- and untreated-bran diets for 9 d. During the last 6 d of this period Zn intakes and faecal and urinary Zn were measured in order to calculate apparent Zn retention. True Zn retention was measured by taking into account losses of Zn of endogenous origin (labelled with 65Zn), by measuring faecal and urinary 65Zn taking the mean specific radioactivity of Zn in kidneys and upper small intestine to represent specific radioactivity of endogenous origin.3. Heat treatment of bran removed approximately one-third of the phytate, but this was not enough to improve Zn absorption from the diet. True Zn retention measured by isotope dilution was significantly higher (P < 0.02) than apparent Zn retention measured bv the conventional balance technique.4. The hypothesis that a reduction in particle size of bran would improve mineral availability was tested by feeding coarse and milled bran (100 g/kg diet) in a semi-synthetic diet to rats and measuring true Fe and apparent Zn absorptions. The importance of phytate was also investigated by feedino a diet containing dephytinized bran.5. Male Wistar rats (mean weight 172 g) were given diets containing coarse, milled or dephytinized bran for 9 d. Fe and Zn intakes were measured and faeces and urine collected for Fe and Zn analysis.6. The mean (±SE) particle size of the bran was reduced on milling from 3.5 (± 1.8) to 0.2–0.5 mm. There were no differences in the fraction of Fe retained between the three groups. Particle size had a small effect on Zn retention which was marginally higher in rats on the milled-bran diet (0.126 (± 0.023)) than in those on the coarse-bran diet (0.087 (± 0.012)). Total removal of phytate had a greater effect and apparent Zn retention from the dephytinized-bran diet was significantly higher (0.182 (±0.027), P < 0.01).

Download Full-text

Protein and energy metabolism of young male Wistar rats fed conjugated linoleic acid as structured triacylglycerol

Archives of Animal Nutrition ◽

10.1080/1745039x.2010.486594 ◽

2010 ◽

Vol 64 (4) ◽

pp. 322-336 ◽

Cited By ~ 6

Author(s):

Henry Jørgensen ◽

Christina Hørup Hansen ◽

Huiling Mu † ◽

Kirsten Jakobsen

Keyword(s):

Linoleic Acid ◽

Energy Metabolism ◽

Conjugated Linoleic Acid ◽

Wistar Rats ◽

Young Male ◽

Male Wistar Rats ◽

Structured Triacylglycerol

Download Full-text

Induction of muricidal behavior by ACTH or adrenalectomy in young male wistar rats

Brain Research Bulletin ◽

10.1016/0361-9230(94)00175-z ◽

1995 ◽

Vol 36 (2) ◽

pp. 119-123 ◽

Cited By ~ 7

Author(s):

S. Miachon ◽

B. Claustrat ◽

R. Cespuglio

Keyword(s):

Wistar Rats ◽

Young Male ◽

Muricidal Behavior ◽

Male Wistar Rats

Download Full-text

The effects of the intraamygdalar melatonin injections on the anxiety like behavior and the spatial memory performance in male Wistar rats

Behavioural Brain Research ◽

10.1016/j.bbr.2011.03.029 ◽

2011 ◽

Vol 222 (1) ◽

pp. 141-150 ◽

Cited By ~ 31

Author(s):

Alper Karakaş ◽

Hamit Coşkun ◽

Aliye Kaya ◽

Ayşegül Kücük ◽

Bülent Gündüz

Keyword(s):

Spatial Memory ◽

Wistar Rats ◽

Memory Performance ◽

Male Wistar Rats

Download Full-text

Effect of Heweianshen Decoction on Orexin-A and Cholecystokinin-8 Expression in Rat Models of Insomnia

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/8034263 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4

Author(s):

Fagen Li ◽

Shaodan Li ◽

Yi Liu ◽

Ke Cao ◽

Minghui Yang

Keyword(s):

Rat Model ◽

Wistar Rats ◽

High Dose ◽

Intragastric Administration ◽

Group Model ◽

Model Group ◽

Orexin A ◽

Blank Group ◽

Rat Models ◽

Male Wistar Rats

Objective. To study the effect of Heweianshen decoction (HAD) on orexin-A and cholecystokinin-8 (CCK-8) expression in rat models of insomnia caused by injecting parachlorophenylalanine (PCPA) intraperitoneally.Methods. Fifty male Wistar rats were randomly divided into five groups (10 rats in each group): blank group, model group, and low-, medium-, and high-dose HAD-treated groups. A rat model of insomnia was established by injecting intraperitoneally with PCPA (300 mg/kg body weight). Rats were given normal saline (10 mL/kg) or 5.25, 10.5, and 21 g/kg HAD by intragastric administration once a day for 6 days. After that, the rats were sacrificed to collect the hypothalamus for tests, using radioimmunoassay to detect the expression of orexin-A and CCK-8.Results. Heweianshen decoction reduced the expression of orexin-A and increased the expression of CCK-8 in the hypothalamus of rat model of insomnia.Conclusion. The therapeutic effect of HAD on insomnia is partially attributed to the decreased expression of orexin-A and increased expression of CCK-8.

Download Full-text

Hepatotherapeutic Tendency of Citrullus lanatus Rind Methanolic Extract on Liver Markers in Male Wistar Rats

Asian Journal of Advanced Research and Reports ◽

10.9734/ajarr/2019/v4i130101 ◽

2019 ◽

pp. 1-10

Author(s):

Ilochi Ogadinma ◽

Chuemere Arthur Nwafor ◽

Bassy Samuel

Keyword(s):

Liver Function ◽

Wistar Rats ◽

Liver Enzymes ◽

Methanolic Extract ◽

Citrullus Lanatus ◽

Left Ventricular ◽

Normal Male ◽

High Dose ◽

Serum Total Bilirubin ◽

Male Wistar Rats

It is a common practice to dispose the peel or rind of fruits. Interestingly, some parts of fruits humans find unacceptable to eat actually possess bioactive nutrients that can be used for medicinal purposes. The effect of methanolic extract of Citrullus lanatus rind on liver function in normal male wistar rats was studied. 24 wistar rats with body weight between 150-250 g were used for this study. The animals were randomly divided into four groups, with 6 rats in each. Group 1 contained the control given normal saline and feed; group 2, low dose, 50mg/kg of methanolic extract of Citrullus lanatus rind was administered, group 3 and 4 were administered medium and high dose of Citrullus lanatus rind extract 100 mg/kg and 200 mg/kg respectively. The Citrullus lanatus rind extract was administered via orogastric route and the experiment lasted for a period of 56 days. Blood samples were collected by left ventricular cardiac puncture for liver function test at the last day of the experiment. The outcome of this research revealed that medium and high dose administration of citrullus lanatus rind significantly (p≤0.05) reduced the serum level of liver enzymes alanine transaminase, aspartate transaminase and alkaline phosphatase and also total protein.There was a non-significant (P≤0.05) change in serum total bilirubin and albumin when all doses were compared to the control. Prolonged and moderate ingestion of Citrullus lanatus rind may be of benefit in regulating blood level of liver enzymes; hence, this part of the fruit has therapeutic value.

Download Full-text