scholarly journals IN VITRO STUDIES OF ELECTROCHEMICAL PURIFICATION OF FOUNDATION SOIL FROM OIL PRODUCTS

2019 ◽  
Vol 21 (4) ◽  
pp. 168-180
Author(s):  
E. A. Shabanov ◽  
S. M. Prostov ◽  
O. V. Gerasimov
Keyword(s):  
Electric Current ◽  
Contaminated Soils ◽  
Electrical Resistance ◽  
Physical And Mechanical Properties ◽  
Polluted Soil ◽  
Oil Products ◽  
Soil Decontamination ◽  
Foundation Soil ◽  
Laboratory Setup

Purpose: The aim of this work is the operational electrophysical method of the control for contamination and purification of soil from oil products and the identification of changes in the soil properties in electrochemical processing to determine rational modes of processing.Methods: Investigations are performed on the laboratory setup including the two -electrode scheme of electric treatment of the artificially polluted soil .The setup is equipped with a system of micro sensors of specific electrical resistance. Traditional laboratory tests are used to determine the physical and mechanical properties of soils. The possibility of control for soil purification by electrochemical method using a system of continuous operational geophysical monitoring is considered.Results: Changes in the physical properties of contaminated soils are experimentally confirmed when exposed to the electric current. A physical model is created for coagulation of oil products in pores during the electrical processing. It increases the amount of sand and clay components with changes in the electrical resistance of soil. The physical processes in the clean and polluted soil are compared. It is shown that when electric current passes through contaminated soils, oil products in pores transfer to a solid, less toxic state, which leads to a change in the specific electrical soil resistance throughout the zone processed. This pattern should be used to control soil decontamination.

Applications of Bentonite to Soil Decontamination

2018 ◽  
Vol 69 (7) ◽  
pp. 1695-1698
Author(s):  
Marin Rusanescu ◽  
Carmen Otilia Rusanescu ◽  
Gheorghe Voicu ◽  
Mihaela Begea
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Laboratory Experiments ◽  
Experimental Studies ◽  
Heavy Metal Concentration ◽  
Polluted Soil ◽  
Raw Material ◽  
Soil Decontamination ◽  
Metal Retention ◽  
Removal Of Heavy Metals

A calcium bentonite from Orasu Nou deposit (Satu Mare Romania) was used as raw material. We have conducted laboratory experiments to determine the influence of bentonite on the degree of heavy metal retention. It has been observed that the rate of retention increases as the heavy metal concentration decreases. Experimental studies have been carried out on metal retention ( Zn) in bentonite. In this paper, we realized laboratory experiments for determining the influence of metal (Zn) on the growth and development of two types of plants (Pelargonium domesticum and Kalanchoe) and the effect of bentonite on the absorption of pollutants. These flowers were planted in unpolluted soil, in heavy metal polluted soil and in heavy metal polluted soil to which bentonite was added to observe the positive effect of bentonite. It has been noticed that the flowers planted in unpolluted soil and polluted with heavy metals to which bentonite has been added, the flowers have flourished, the leaves are still green and the plants whose soils have been polluted with heavy metals began to dry after 6 days, three weeks have yellowish leaves and flowers have dried. Experiments have demonstrated the essential role of bentonite for the removal of heavy metals polluted soil.

scholarly journals Thermal and non-thermal effects of capacitive–resistive electric transfer application on different structures of the knee: a cadaveric study

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jacobo Rodríguez-Sanz ◽  
Albert Pérez-Bellmunt ◽  
Carlos López-de-Celis ◽  
Orosia María Lucha-López ◽  
Vanessa González-Rueda ◽  
...  
Keyword(s):  
Low Power ◽  
Electric Current ◽  
Thermal Effects ◽  
Sports Medicine ◽  
Current Flow ◽  
In Vitro Study ◽  
Science Data ◽  
Fresh Frozen ◽  
Inflammatory Processes

AbstractCapacitive–resistive electric transfer therapy is used in physical rehabilitation and sports medicine to treat muscle, bone, ligament and tendon injuries. The purpose is to analyze the temperature change and transmission of electric current in superficial and deep knee tissues when applying different protocols of capacitive–resistive electric transfer therapy. Five fresh frozen cadavers (10 legs) were included in this study. Four interventions (high/low power) were performed for 5 min by a physiotherapist with experience. Dynamic movements were performed to the posterior region of the knee. Capsular, intra-articular and superficial temperature were recorded at 1-min intervals and 5 min after the treatment, using thermocouples placed with ultrasound guidance. The low-power protocols had only slight capsular and intra-capsular thermal effects, but electric current flow was observed. The high-power protocols achieved a greater increase in capsular and intra-articular temperature and a greater current flow than the low-power protocols. The information obtained in this in vitro study could serve as basic science data to hypothesize capsular and intra-articular knee recovery in living subjects. The current flow without increasing the temperature in inflammatory processes and increasing the temperature of the tissues in chronic processes with capacitive–resistive electric transfer therapy could be useful for real patients.

scholarly journals Influences of advanced glycosylation end products on the inner blood–retinal barrier in a co-culture cell model in vitro

2020 ◽  
Vol 15 (1) ◽  
pp. 619-628
Author(s):  
Chen Yuan ◽  
Ya Mo ◽  
Jie Yang ◽  
Mei Zhang ◽  
Xuejun Xie
Keyword(s):  
Electrical Resistance ◽  
Cell Model ◽  
Polyclonal Antibodies ◽  
Time Dependent ◽  
Dependent Manner ◽  
Blood Retinal Barrier ◽  
Advanced Glycosylation End Products ◽  
End Products ◽  
Advanced Glycosylation

AbstractAdvanced glycosylation end products (AGEs) are harmful factors that can damage the inner blood–retinal barrier (iBRB). Rat retinal microvascular endothelial cells (RMECs) were isolated and cultured, and identified by anti-CD31 and von Willebrand factor polyclonal antibodies. Similarly, rat retinal Müller glial cells (RMGCs) were identified by H&E staining and with antibodies of glial fibrillary acidic protein and glutamine synthetase. The transepithelial electrical resistance (TEER) value was measured with a Millicell electrical resistance system to observe the leakage of the barrier. Transwell cell plates for co-culturing RMECs with RMGCs were used to construct an iBRB model, which was then tested with the addition of AGEs at final concentrations of 50 and 100 mg/L for 24, 48, and 72 h. AGEs in the in vitro iBRB model constructed by RMEC and RMGC co-culture led to the imbalance of the vascular endothelial growth factor (VEGF) and pigment epithelial derivative factor (PEDF), and the permeability of the RMEC layer increased because the TEER decreased in a dose- and time-dependent manner. AGEs increased VEGF but lowered PEDF in a dose- and time-dependent manner. The intervention with AGEs led to the change of the transendothelial resistance of the RMEC layer likely caused by the increased ratio of VEGF/PEDF.

scholarly journals Effect of Propolis Preparations on Transepithelial Electrical Potential, Resistance, and Ion Transport in In Vitro Study

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Paulina Smyk ◽  
Iga Hołyńska-Iwan ◽  
Dorota Olszewska-Słonina
Keyword(s):  
Electrical Resistance ◽  
Ussing Chamber ◽  
Electrical Potential ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
Ringer Solution ◽  
Chloride Ions ◽  
Propolis Extract ◽  
Ions Transport

Background. Propolis and its ethanol extract show positive germicidal, bacteriostatic, and anti-inflammatory antioxidants and regenerative properties after use on the surface of the skin. Propolis is in common use in production of cosmetics and in folk medicine. The influence of this resinous mixture on ion channels, channels located in skin cells membranes and skin electrical resistance, was not explained. Objective. The main aim of the study was the evaluation of electrophysiological skin parameters during mechanical and chemical-mechanical stimulation after use of ethanol extract of propolis and propolis ointment in comparison with iso-osmotic Ringer solution. Methods. Skin fragments were taken from white New Zealand rabbits and distributed into three experimental groups which were incubated in ethanol extract of propolis (EEP), propolis ointment, and Ringer solution. Then they were placed in a Ussing chamber to measure electrophysiological parameters values. Results. In this study the influence of EEP on changes in value of electrical potential during block of chloride ions transport at the same time was observed. Ethanol propolis extract dissolved in water increases the transepidermal sodium ions transport in contrast to propolis ointment. Conclusion. The way of preparation cosmetics, which contain propolis, has effects on transepidermal ions transport in the rabbit’s skin. The value of skin electrical resistance is changing with penetration depth of active propolis substances contained in cosmetics.

Coupling in vitro assays with sequential extraction to investigate cadmium bioaccessibility in contaminated soils

Chemosphere ◽  
2021 ◽  
pp. 132655
Author(s):  
Shi–Wei Li ◽  
Minghui Chang ◽  
Xiaoyue Huang ◽  
Helian Li ◽  
Hong–Bo Li ◽  
...  
Keyword(s):  
Sequential Extraction ◽  
Contaminated Soils ◽  
In Vitro Assays

scholarly journals In vitro growth and indoleacetic acid production by Mesorhizobium loti SEMIA806 and SEMIA816 under the influence of copper ions

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Jéssica Dutra Vieira ◽  
Paulo Roberto Diniz Da Silva ◽  
Valdir Marcos Stefenon
Keyword(s):  
Contaminated Soils ◽  
Acid Production ◽  
Indoleacetic Acid ◽  
Copper Ions ◽  
Control Culture ◽  
Control Treatment ◽  
Symbiotic Interaction ◽  
Mesorhizobium Loti ◽  
Significant Difference

The indoleacetic acid produced by symbiotic bacteria is an important phytohormone signaling microbe-plant interaction, being therefore essential for rhizoremediation. In this study, the effect of different concentrations of copper ions on the bacterial growth and indoleacetic acid production was investigated in two strains of Mesorhizobium loti in in vitro conditions, aiming to determine critical concentrations of this heavy metal for rhizoremediation of contaminated soils using this bacterium. The experiment consisted on a control culture without copper and three treatments supplemented with 10 mg.L-1, 20 mg.L-1 or 50 mg.L-1 of CuSO4. For both strains, the growth stopped after 48h and no significant difference was observed across treatments. The production of indoleacetic acid by the control treatment without copper was significantly higher in comparison to the copper- containing treatments. Mesorhizobium loti SEMIA806 and SEMIA816 are resistant to up to 50 mg.L-1 of CuSO4 in the culture medium, presenting effective growth. The synthesis of indoleacetic acid was strongly reduced but not excluded by ions copper in the medium. So, it is expected that environmental copper found in the soil up to the concentration of 50 mg.L-1 will not preclude the symbiotic interaction between M. loti and leguminous host plant in rhizoremediation enterprises.

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