In vitro-digested milk proteins: Evaluation of angiotensin-1-converting enzyme inhibitory and antioxidant activities, peptidomic profile, and mucin gene expression in HT29-MTX cells

The present study investigated angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities of barbel muscle protein hydrolysate prepared with Alcalase. The barbel muscle protein hydrolysate displayed a high ACE inhibitory activity (CI50=0.92 mg/mL). The antioxidant activities of protein hydrolysate at different concentrations were evaluated using variousin vitroantioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method and reducing power assay. The barbel muscle protein hydrolysate exhibited an important radical scavenging effect and reducing power. These results obtained byin vitrosystems obviously established the antioxidant potency of barbel hydrolysate to donate electron or hydrogen atom to reduce the free radical. Furthermore, these bioactive substances can be exploited into functional foods or used as source of nutraceuticals.

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Production of Ulvan Oligosaccharides with Antioxidant and Angiotensin-Converting Enzyme-Inhibitory Activities by Microbial Enzymatic Hydrolysis

Fermentation ◽

10.3390/fermentation7030160 ◽

2021 ◽

Vol 7 (3) ◽

pp. 160

Author(s):

Yueh-Hao Ronny Hung ◽

Guan-Wen Chen ◽

Chorng-Liang Pan ◽

Hong-Ting Victor Lin

Keyword(s):

Enzymatic Hydrolysis ◽

Antioxidant Activities ◽

Ace Inhibition ◽

Aeromonas Salmonicida ◽

Water Soluble ◽

Hot Water ◽

Converting Enzyme ◽

Green Seaweed ◽

Ft Ir

Seaweed oligosaccharides have attracted attention in food, agricultural, and medical applications recently. Compared to red and brown seaweeds, fewer studies have focused on the biological activity of green seaweed’s oligosaccharides. This study aimed to produce bioactive ulvan oligosaccharides via enzymatic hydrolysis from green seaweed Ulva lactuca. Ulvan, a water-soluble polysaccharide, was obtained by hot water extraction. Two isolated marine bacteria, Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108, were used to produce multiple hydrolases, such as ulvanolytic enzymes, amylase, cellulase, and xylanase, to degrade the ulvan extract. An ultrafiltration system was used to separate the enzymatic hydrolysate to acquire the ulvan oligosaccharides (UOS). The characteristics of the ulvan extract and the UOS were determined by yield, reducing sugar, uronic acid, sulfate group, and total phenols. The FT-IR spectrum indicated that the ulvan extract and the UOS presented the bands associated with O-H, C=O, C-O, and S=O stretching. Angiotensin I converting enzyme (ACE) inhibition and antioxidant activities in vitro were evaluated in the ulvan extract and the UOS. These results provide a practical approach to producing bioactive UOS by microbial enzymatic hydrolysis that can benefit the development of seaweed-based products at the industrial scale.

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Amelioration of Cigarette Smoke-Induced Mucus Hypersecretion and Viscosity by Dendrobium officinale Polysaccharides In Vitro and In Vivo

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/8217642 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Rui Chen ◽

Yingmin Liang ◽

Mary Sau Man Ip ◽

Kalin Yanbo Zhang ◽

Judith Choi Wo Mak

Keyword(s):

Gene Expression ◽

Cigarette Smoke ◽

Secretory Granules ◽

Dendrobium Officinale ◽

In Vivo Models ◽

Mucus Hypersecretion ◽

Mucin Gene ◽

Starting Point

Chronic obstructive pulmonary disease (COPD), characterized by oxidative stress and inflammation, is one of the leading causes of death worldwide, in which cigarette smoke (CS) is the major risk factor. Dendrobium officinale polysaccharides (DOPs) are the main active ingredients extracted from Dendrobium officinale, which have been reported to have antioxidant and anti-inflammatory activity as well as inhibition of mucin gene expression. This study is aimed at investigating the effect of DOPs on CS-induced mucus hypersecretion and viscosity in vitro and in vivo. For in vitro study, primary normal human bronchial epithelial cells (HBECs) differentiated at the air-liquid interface (ALI) culture for 28 days were stimulated with cigarette smoke medium (CSM) in the absence or presence of various concentrations of DOPs or N-acetylcysteine (NAC) for 24 hours. For in vivo study, male Sprague-Dawley rats were randomized to sham air (SA) as control group or CS group for 56 days. At day 29, rats were subdivided and given water as control, DOPs, or NAC as positive control as a mucolytic drug via oral gavage for the remaining duration. Samples collected from apical washing, cell lysates, bronchoalveolar lavage (BAL), and lung tissues were evaluated for mucin gene expression, mucus secretion, and viscosity. DOPs ameliorated the CS-induced mucus hypersecretion and viscosity as shown by the downregulation of MUC5AC mRNA, MUC5AC secretary protein, and mucus viscosity via inhibition of mucus secretory granules in both in vitro and in vivo models. DOPs produced its effective effects on the CS-induced mucus hypersecretion and viscosity via the inhibition of the mucus secretory granules. These findings could be a starting point for considering the potential role of DOPs in the management of the smoking-mediated COPD. However, further research is needed.

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Effect of retinoic acid on mucin gene expression in rat airways in vitro

Biochemical Journal ◽

10.1042/bj2980759b ◽

1994 ◽

Vol 298 (3) ◽

pp. 759-759

Keyword(s):

Gene Expression ◽

Retinoic Acid ◽

Mucin Gene

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Effect of In Vitro Digestion on the Antioxidant and Angiotensin Converting Enzyme (ACE)-Inhibitory Potential of Buffalo Milk Processed Cheddar Cheese

10.20944/preprints202106.0528.v1 ◽

2021 ◽

Author(s):

Amal Shaukat ◽

Muhammad Nadeem ◽

Tahir Mahmood Qureshi ◽

Rabiak Kanwal ◽

Muhammad Sultan ◽

...

Keyword(s):

Angiotensin Converting Enzyme ◽

Antioxidant Activities ◽

Cheddar Cheese ◽

In Vitro Digestion ◽

Ace Inhibition ◽

Black Pepper ◽

Buffalo Milk ◽

Converting Enzyme ◽

Freeze Dried

The purpose of this study was to develop an in-vitro digestion protocol to evaluate the antioxidant potential of the peptides found in processed cheddar cheese using digestion enzymes. We studied first antioxidant and angiotensin converting enzyme (ACE) inhibition and antioxidant activities of processed cheddar cheese with the addition of spices e.g. cumin, clove and black pepper made from buffalo milk and ripened for 9 months. Then we conducted an in vitro digestion of processed cheddar cheese by gastric and duodenal enzymes. Freeze dried water (WSE) and ethanol soluble fractions (ESE) of processed cheddar cheese were also monitored for their ACE inhibition activity and antioxidant activities. In our preliminary experiments, different levels of spices (cumin, clove and black pepper) were tested into cheese matrix and only one level 0.2g/100g (0.2%) on the basis of cheese weight was considered good concerning sensory evaluation. Significant increase in ACE-inhibition (%) of processed Cheddar cheese as well as its WSE and ESE was obtained. Lower IC50 values were found after duodenal phase digestion compared to oral phase digestion.

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Shigella dysenteriae Modulates BMP Pathway to Induce Mucin Gene Expression In Vivo and In Vitro

PLoS ONE ◽

10.1371/journal.pone.0111408 ◽

2014 ◽

Vol 9 (11) ◽

pp. e111408 ◽

Cited By ~ 6

Author(s):

Ashidha Gopal ◽

Soumya Chidambaram Iyer ◽

Udhayakumar Gopal ◽

Niranjali Devaraj ◽

Devaraj Halagowder

Keyword(s):

Gene Expression ◽

Shigella Dysenteriae ◽

Mucin Gene ◽

Bmp Pathway

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Effect of In Vitro Digestion on the Antioxidant and Angiotensin-Converting Enzyme Inhibitory Potential of Buffalo Milk Processed Cheddar Cheese

Foods ◽

10.3390/foods10071661 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1661

Author(s):

Amal Shaukat ◽

Muhammad Nadeem ◽

Tahir Mahmood Qureshi ◽

Rabia Kanwal ◽

Muhammad Sultan ◽

...

Keyword(s):

Angiotensin Converting Enzyme ◽

Antioxidant Activities ◽

Cheddar Cheese ◽

In Vitro Digestion ◽

Ace Inhibition ◽

Black Pepper ◽

Buffalo Milk ◽

Converting Enzyme ◽

Inhibitory Potential

The purpose of this study was to develop an in-vitro digestion protocol to evaluate the antioxidant potential of the peptides found in processed cheddar cheese using digestion enzymes. We first studied antioxidant and angiotensin-converting enzyme (ACE) inhibition and antioxidant activities of processed cheddar cheese with the addition of spices e.g., cumin, clove, and black pepper made from buffalo milk and ripened for 9 months. Then we conducted an in vitro digestion of processed cheddar cheese by gastric and duodenal enzymes. Freeze-dried water (WSE) and ethanol-soluble fractions (ESE) of processed cheddar cheese were also monitored for their ACE inhibition activity and antioxidant activities. In our preliminary experiments, different levels of spices (cumin, clove, and black pepper) were tested into a cheese matrix and only one level 0.2 g/100 g (0.2%) based on cheese weight was considered good after sensory evaluation. Findings of the present study revealed that ACE-inhibitory potential was the highest in processed cheese made from buffalo milk with the addition of 0.2% cumin, clove, and black pepper. A significant increase in ACE-inhibition (%) of processed cheddar cheese, as well as its WSE and ESE, was obtained. Lower IC50 values were found after duodenal phase digestion compared to oral phase digestion.

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RNA-seq analysis of bronchial organoids reveals ACE2 expression levels before and after SARS-CoV-2 infection

10.31219/osf.io/hdwrp ◽

2020 ◽

Author(s):

Edward Chen

Keyword(s):

Gene Expression ◽

Respiratory Symptoms ◽

Lung Cells ◽

Converting Enzyme ◽

Rna Seq ◽

Angiotensin Converting Enzyme 2 ◽

Expression Levels ◽

Before And After ◽

Insight Into

The SARS-CoV-2 virus invades human cells via the angiotensin-converting enzyme 2 (ACE2)receptor. ACE2 expression in the lung cells creates a target for the SARS-CoV-2 virus, andconsequently infected individuals often see respiratory symptoms. Here, RNA sequencing(RNA-seq) datasets from in vitro human bronchial organoids (hBO) are analyzed to identifyunderlying gene expression changes as a result of an infection by SARS-CoV-2. Throughdifferential expression analysis, it was found that ACE2 expression levels did not changesignificantly after SARS-CoV-2 infection, but other genes that encode for major immunesignaling pathways were upregulated. This study can lend insight into potential targets for drugdevelopment and treatment for the COVID-19 disease.

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In vitro Angiotesin-1-converting enzyme, α-amylase and α-glucosidase inhibitory and antioxidant activities of Luffa cylindrical (L.) M. Roem seed protein hydrolysate

Heliyon ◽

10.1016/j.heliyon.2019.e01634 ◽

2019 ◽

Vol 5 (5) ◽

pp. e01634 ◽

Cited By ~ 7

Author(s):

Rotimi Olusanya Arise ◽

Jalil James Idi ◽

Iseoluwa Maureen Mic-Braimoh ◽

Emmanuel Korode ◽

Risikat Nike Ahmed ◽

...

Keyword(s):

Seed Protein ◽

Antioxidant Activities ◽

Protein Hydrolysate ◽

Converting Enzyme

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Phytochemicals and In Vitro Bioactivities of Aqueous Ethanolic Extracts from Common Vegetables in Thai Food

Plants ◽

10.3390/plants10081563 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1563

Author(s):

Uthaiwan Suttisansanee ◽

Parunya Thiyajai ◽

Parisut Chalermchaiwat ◽

Khanitha Wongwathanarat ◽

Kanchana Pruesapan ◽

...

Keyword(s):

Angiotensin Converting Enzyme ◽

Fruits And Vegetables ◽

Antioxidant Activities ◽

Enzymatic Reaction ◽

Solanum Melongena ◽

World Health ◽

Converting Enzyme ◽

Plant Samples ◽

Health Organization

Non-communicable diseases (NCDs) are the leading global cause of death. The World Health Organization (WHO) has endorsed the consumption of fruits and vegetables because they are rich in phytochemicals that sustainably ameliorate the occurrence of NCDs. Thai food contains many spices and vegetables with recognized health benefits. Quality control of plant samples encountered a bottleneck in the field and comparative studies of plant control origins including species or cultivar identification, growing area and appropriate harvesting time are limited. To address this issue, all plant samples used in this study were cultivated and controlled by the Department of Agriculture, Ministry of Agriculture and Cooperatives, Thailand. The samples were phytochemically screened and determined their health-promoting bioactivities via antioxidant activities and inhibition of NCD-related enzymes including lipase (obesity), α-amylase and α-glucosidase (diabetes), angiotensin-converting enzyme (hypertension), as well as acetylcholinesterase, butyrylcholinesterase and β-secretase (Alzheimer’s disease). The non-enzymatic reaction toward glycation was also evaluated. The results showed that Senegalia pennata subsp. insuavis (Lace) Maslin, Seigler & Ebinger, Citrus hystrix DC. and Solanum melongena ‘Kermit’ extracts exhibited high antioxidant activities. Moreover, Citrus hystrix DC. extract was a potent inhibitor against lipase, angiotensin-converting enzyme and butyrylcholinesterase, while Coriandrum sativum L. and Psophocarpus tetragonolobus (L.) DC. were potent anti-diabetic agents and Senegalia pennata subsp. insuavis (Lace) Maslin, and Seigler & Ebinger was a potent anti-glycation agent. Our data provide a comparative analysis of ten vegetables to encourage healthy food consumption and development to control NCDs in Thailand in the future.

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