scholarly journals Effect of In Vitro Digestion on the Antioxidant and Angiotensin-Converting Enzyme Inhibitory Potential of Buffalo Milk Processed Cheddar Cheese

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1661
Author(s):  
Amal Shaukat ◽  
Muhammad Nadeem ◽  
Tahir Mahmood Qureshi ◽  
Rabia Kanwal ◽  
Muhammad Sultan ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Antioxidant Activities ◽  
Cheddar Cheese ◽  
In Vitro Digestion ◽  
Ace Inhibition ◽  
Black Pepper ◽  
Buffalo Milk ◽  
Converting Enzyme ◽  
Inhibitory Potential

The purpose of this study was to develop an in-vitro digestion protocol to evaluate the antioxidant potential of the peptides found in processed cheddar cheese using digestion enzymes. We first studied antioxidant and angiotensin-converting enzyme (ACE) inhibition and antioxidant activities of processed cheddar cheese with the addition of spices e.g., cumin, clove, and black pepper made from buffalo milk and ripened for 9 months. Then we conducted an in vitro digestion of processed cheddar cheese by gastric and duodenal enzymes. Freeze-dried water (WSE) and ethanol-soluble fractions (ESE) of processed cheddar cheese were also monitored for their ACE inhibition activity and antioxidant activities. In our preliminary experiments, different levels of spices (cumin, clove, and black pepper) were tested into a cheese matrix and only one level 0.2 g/100 g (0.2%) based on cheese weight was considered good after sensory evaluation. Findings of the present study revealed that ACE-inhibitory potential was the highest in processed cheese made from buffalo milk with the addition of 0.2% cumin, clove, and black pepper. A significant increase in ACE-inhibition (%) of processed cheddar cheese, as well as its WSE and ESE, was obtained. Lower IC50 values were found after duodenal phase digestion compared to oral phase digestion.

scholarly journals Effect of In Vitro Digestion on the Antioxidant and Angiotensin Converting Enzyme (ACE)-Inhibitory Potential of Buffalo Milk Processed Cheddar Cheese

2021 ◽  
Author(s):  
Amal Shaukat ◽  
Muhammad Nadeem ◽  
Tahir Mahmood Qureshi ◽  
Rabiak Kanwal ◽  
Muhammad Sultan ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Antioxidant Activities ◽  
Cheddar Cheese ◽  
In Vitro Digestion ◽  
Ace Inhibition ◽  
Black Pepper ◽  
Buffalo Milk ◽  
Converting Enzyme ◽  
Freeze Dried

The purpose of this study was to develop an in-vitro digestion protocol to evaluate the antioxidant potential of the peptides found in processed cheddar cheese using digestion enzymes. We studied first antioxidant and angiotensin converting enzyme (ACE) inhibition and antioxidant activities of processed cheddar cheese with the addition of spices e.g. cumin, clove and black pepper made from buffalo milk and ripened for 9 months. Then we conducted an in vitro digestion of processed cheddar cheese by gastric and duodenal enzymes. Freeze dried water (WSE) and ethanol soluble fractions (ESE) of processed cheddar cheese were also monitored for their ACE inhibition activity and antioxidant activities. In our preliminary experiments, different levels of spices (cumin, clove and black pepper) were tested into cheese matrix and only one level 0.2g/100g (0.2%) on the basis of cheese weight was considered good concerning sensory evaluation. Significant increase in ACE-inhibition (%) of processed Cheddar cheese as well as its WSE and ESE was obtained. Lower IC50 values were found after duodenal phase digestion compared to oral phase digestion.

scholarly journals ANGIOTENSIN CONVERTING ENZYME INHIBITION POTENTIAL OF ANNAPAVALA CHENDHURAM FOR THE TREATMENT OF HYPERTENSION - AN IN–VITRO ASSAY

AYUSHDHARA ◽  
2020 ◽  
pp. 2599-2604
Author(s):  
Sabari Girija N ◽  
Sinekha M A ◽  
Guptaj S ◽  
Shanmugapriya P ◽  
Madhavan R
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Ace Inhibition ◽  
In Vitro Assay ◽  
Lifestyle Changes ◽  
Angiotensin Converting Enzyme Inhibition ◽  
Uv Spectrophotometry ◽  
Test Drug ◽  
Converting Enzyme ◽  
Vitro Assay

Hypertension is that the most noteworthy risk factor for cardiovascular diseases and stroke. Dietary and lifestyle changes play the foremost part to decrease the hazard of hypertension and other related wellbeing complications. Angiotensin Converting Enzyme (ACE) inhibitors play a major role in treating hypertension. Annapavala chendhuram is a herbo – mineral Siddha formulation comes under the type of 32 internal medicines of Siddha. Hypolipidemic activity of Annapavala chendhuram has been proven by some research studies. Hence, the purpose of the present study was to evaluate the ACE inhibition activity on Annapavala chendhuram by using an in-vitro assay. The ACE inhibition assay was evaluated by UV Spectrophotometry technique based on the hydrolysis of histidyl-hippuryl-leucine (HHL) by ACE. About 50µL test sample with varying concentration (100- 500 µg/ml) along with standard captopril (100µg/ml) added with 50µL of ACE and some process had continued. The present study indicates that the test drug Annapavala chendhuram was effective in inhibiting the enzyme ACE dose-dependently. Maximum percentage inhibition of about 53.24±8.403% was observed at 500μg/ml when compared to that of the Captopril, a standard ACE enzyme inhibitor agent with the maximum inhibition 86.98 ± 6.375 at the concentration of 100μg/ml. It was concluded that the test drug Annapavala chendhuram possess significant anti-hypertensive property in protein denaturation assay. So, further in-vitro evaluation of ACE inhibitory activity on Siddha herbal preparations and clinical trials will be the need of the hour.

scholarly journals Production of Ulvan Oligosaccharides with Antioxidant and Angiotensin-Converting Enzyme-Inhibitory Activities by Microbial Enzymatic Hydrolysis

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 160
Author(s):  
Yueh-Hao Ronny Hung ◽  
Guan-Wen Chen ◽  
Chorng-Liang Pan ◽  
Hong-Ting Victor Lin
Keyword(s):  
Enzymatic Hydrolysis ◽  
Antioxidant Activities ◽  
Ace Inhibition ◽  
Aeromonas Salmonicida ◽  
Water Soluble ◽  
Hot Water ◽  
Converting Enzyme ◽  
Green Seaweed ◽  
Ft Ir

Seaweed oligosaccharides have attracted attention in food, agricultural, and medical applications recently. Compared to red and brown seaweeds, fewer studies have focused on the biological activity of green seaweed’s oligosaccharides. This study aimed to produce bioactive ulvan oligosaccharides via enzymatic hydrolysis from green seaweed Ulva lactuca. Ulvan, a water-soluble polysaccharide, was obtained by hot water extraction. Two isolated marine bacteria, Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108, were used to produce multiple hydrolases, such as ulvanolytic enzymes, amylase, cellulase, and xylanase, to degrade the ulvan extract. An ultrafiltration system was used to separate the enzymatic hydrolysate to acquire the ulvan oligosaccharides (UOS). The characteristics of the ulvan extract and the UOS were determined by yield, reducing sugar, uronic acid, sulfate group, and total phenols. The FT-IR spectrum indicated that the ulvan extract and the UOS presented the bands associated with O-H, C=O, C-O, and S=O stretching. Angiotensin I converting enzyme (ACE) inhibition and antioxidant activities in vitro were evaluated in the ulvan extract and the UOS. These results provide a practical approach to producing bioactive UOS by microbial enzymatic hydrolysis that can benefit the development of seaweed-based products at the industrial scale.

scholarly journals Discovery of Novel Angiotensin-Converting Enzyme Inhibitory Peptides from Todarodes pacificus and Their Inhibitory Mechanism: In Silico and In Vitro Studies

2019 ◽  
Vol 20 (17) ◽  
pp. 4159 ◽  
Author(s):  
Dingyi Yu ◽  
Cong Wang ◽  
Yufeng Song ◽  
Junxiang Zhu ◽  
Xiaojun Zhang
Keyword(s):  
Angiotensin Converting Enzyme ◽  
In Silico ◽  
Ace Inhibition ◽  
Degree Of Hydrolysis ◽  
Converting Enzyme ◽  
Inhibitory Peptides ◽  
Todarodes Pacificus ◽  
Ace Inhibitory Peptides ◽  
Ace Inhibitory

In order to rapidly and efficiently excavate antihypertensive ingredients in Todarodes pacificus, its myosin heavy chain was hydrolyzed in silico and the angiotensin-converting enzyme (ACE) inhibitory peptides were predicted using integrated bioinformatics tools. The results showed the degree of hydrolysis (DH) theoretically achieved 56.8% when digested with papain, ficin, and prolyl endopeptidase (PREP), producing 126 ACE inhibitory peptides. By predicting the toxicity, allergenicity, gastrointestinal stability, and intestinal epithelial permeability, 30 peptides were finally screened, of which 21 had been reported and 9 were new. Moreover, the newly discovered peptides were synthesized to evaluate their in vitro ACE inhibition, showing Ile-Ile-Tyr and Asn-Pro-Pro-Lys had strong effects with a pIC50 of 4.58 and 4.41, respectively. Further, their interaction mechanisms and bonding configurations with ACE were explored by molecular simulation. The preferred conformation of Ile-Ile-Tyr and Asn-Pro-Pro-Lys located in ACE were successfully predicted using the appropriate docking parameters. The molecular dynamics (MD) result indicated that they bound tightly to the active site of ACE by means of coordination with Zn(II) and hydrogen bonding and hydrophobic interaction with the residues in the pockets of S1 and S2, resulting in stable complexes. In summary, this work proposed a strategy for screening and identifying antihypertensive peptides from Todarodes pacificus.

scholarly journals In vitro antidiabetic and inhibitory potential of turmeric (Curcuma longa L) rhizome against cellular and LDL oxidation and angiotensin converting enzyme

2013 ◽  
Vol 51 (12) ◽  
pp. 3910-3917 ◽  
Author(s):  
P. C. Lekshmi ◽  
Ranjith Arimboor ◽  
V. M. Nisha ◽  
A. Nirmala Menon ◽  
K. G. Raghu
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Curcuma Longa ◽  
Ldl Oxidation ◽  
Converting Enzyme ◽  
Inhibitory Potential

EFFECT OF STRAIN ENTEROCOCCUS FAECALIS AN1 ON RELEASE OF BIOACTIVE PEPTIDES FROM WHEY PROTEINS IN IN VITRO SIMULATED GASTROINTESTINAL CONDITIONS

2020 ◽  
Vol 03 (04) ◽  
pp. 64-68
Author(s):  
Rugiya Sabir Mustafayeva ◽  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Enterococcus Faecalis ◽  
Dairy Products ◽  
Bioactive Peptides ◽  
Whey Proteins ◽  
Ace Inhibition ◽  
Converting Enzyme ◽  
Gastrointestinal Conditions ◽  
Casein Hydrolyzate

The aim of the study was to study the potential of the proteolytic strain Enterococcus faecalis AN1 to generate inhibition of angiotensin converting enzyme (ACE), as well as to determine the effect of subsequent hydrolysis with pepsin and pancreatin in vitro simulated gastrointestinal system on this activity. Analysis of substrate hydrolysis and peptide formation was performed using SDS-PAGE and electrophoresis by RP-HPLC. Casein hydrolyzate with proteases of the strain showed the ability to produce peptides with ACE inhibition activity, which shows the use of these strains in the development of functional dairy products with antihypertensive properties. The studied strain has the potential to produce functional dairy products. Key words: lactic acid bacteria, proteases, caseins, bioactive peptides, angiotensin converting enzyme

scholarly journals Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

2021 ◽  
Vol 44 (2) ◽  
pp. 222-228
Author(s):  
T. R. Hakim ◽  
A. Pratiwi ◽  
Jamhari Jamhari ◽  
N. A. Fitriyanto ◽  
Rusman Rusman ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Dry Matter ◽  
Ace Inhibition ◽  
Converting Enzyme ◽  
Inhibition Activity ◽  
Collagen Hydrolysate ◽  
Molecular Weights ◽  
Ic50 Value ◽  
Inhibitory Potential ◽  
Ace Inhibitory

The study was designed to determine the potential of collagen hydrolysate produced from the skin of Kacang goat through chymotrypsin hydrolysis to be used as an inhibitor of angiotensin converting enzyme (ACE). This research was conducted in three replications, with the measured parameters include ACE inhibitory potential and collagen hydrolysate fractionation. The results showed that collagen extraction of Kacang goat skin by chymotrypsin hydrolysis yielded 9.74% (dry matter, v/v) collagen, with pH at 6.6. The extracted collagen contained α1, α2, and β collagen chains with molecular weights of 151 kDa, 141 kDa, and 240 kDa, respectively. Furthermore, the collagen hydrolysis produced protein peptides confirmed at molecular weights of 43 to 107 kDa. The hydrolysate fractionation at molecular weights of <3 kDa, 3-5 kDa, and >5 kDa showed proteins concentrations of 2.33 mg/mL, 3.81 mg/mL, and 3.93 mg/mL, respectively. The hydrolysate fractionation with molecular weight <3 kDa showed to have ACE inhibition activity with the IC50 value of 0.47 mg/mL. The study concluded that collagen hydrolysate extracted from the skin of Kacang goat had a promising potential as a source of antihypertensive agent.

scholarly journals Renal endothelial function is associated with the anti-proteinuric effect of ACE inhibition in 5/6 nephrectomized rats

2016 ◽  
Vol 310 (10) ◽  
pp. F1047-F1053 ◽  
Author(s):  
Simone Vettoretti ◽  
Peter Vavrinec ◽  
Peter Ochodnicky ◽  
Leo E. Deelman ◽  
Dick De Zeeuw ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Endothelial Function ◽  
Renal Injury ◽  
Renal Damage ◽  
Enzyme Inhibitor ◽  
Ace Inhibition ◽  
Variable Degree ◽  
Converting Enzyme ◽  
Variable Response

In healthy rats, the physiological variation of baseline endothelial function of intrarenal arteries correlates with the severity of renal damage in response to a subsequent specific renal injury. However, whether such a variation in endothelial function may also condition or predict the variable response to angiotensin-converting enzyme-inhibiting treatment in these individuals has not been addressed before. To study this, 5/6 nephrectomy was performed to induce renal injury and chronic kidney disease in a group of healthy Wistar rats. At the time of nephrectomy, interlobar arteries were obtained from the extirpated right kidney and studied in vitro for endothelium-dependent relaxation to acetylcholine. Six weeks thereafter, treatment with lisinopril was started ( n = 11) and continued for 9 wk. Proteinuria (metabolic cages) and systolic blood pressure (SBP; tail cuff) were evaluated weekly, and these were analyzed in relation to renal endothelial function at baseline. 5/6 Nephrectomy induced an increase in SBP and progressive proteinuria. Treatment with lisinopril reduced SBP and slowed proteinuria, albeit to a variable degree among individuals. The acetylcholine-induced renal artery dilation at baseline negatively correlated with lisinopril-induced reduction of proteinuria ( r2 = 0.648, P = 0.003) and with the decrease in SBP ( r2 = 0.592, P = 0.006). Our data suggest that angiotensin-converting enzyme-inhibitor attenuates the progression of renal damage the most in those individuals with decreased basal renal endothelial-mediated vasodilation.

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