Isolation and Identification of Some Major Water-soluble Peptides in Feta Cheese

Proteolysis is one of the major biochemical events which takes place during cheese ripening and its degradation products, amino acids and peptides, have a considerable influence on the sensory characteristics of cheese (Urbach, 1993). Primary proteolysis leads to the formation of large water-insoluble peptides and smaller water-soluble peptides. Several peptides from bovine milk cheeses have been isolated and identified, particularly from Cheddar cheese (e.g., McSweeney et al. 1994; Singh et al. 1994, 1995, 1997; Gouldsworthy et al. 1996; Fernández et al. 1998). However, there are few data available on the identification of peptides from ewes’ milk cheeses, although Michaelidou et al. (1998) identified some major peptides in the water-soluble fraction of Feta cheese (ewe's milk cheese).

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Analyzing the Carotenoid Composition of Melilot (Melilotus officinalis (L.) Pall.) Extracts and the Effects of Isolated (All-E)-lutein-5,6-epoxide on Primary Sensory Neurons and Macrophages

Molecules ◽

10.3390/molecules26020503 ◽

2021 ◽

Vol 26 (2) ◽

pp. 503

Author(s):

Györgyi Horváth ◽

Eszter Csikós ◽

Eichertné Violetta Andres ◽

Tímea Bencsik ◽

Anikó Takátsy ◽

...

Keyword(s):

Liquid Chromatography ◽

Sensory Neurons ◽

Biological Activities ◽

Water Soluble ◽

Soluble Form ◽

Primary Sensory Neurons ◽

Isolation And Identification ◽

Melilotus Officinalis ◽

Carotenoid Composition ◽

Anti Inflammatory

Melilotus officinalis is known to contain several types of secondary metabolites. In contrast, the carotenoid composition of this medicinal plant has not been investigated, although it may also contribute to the biological activities of the drug, such as anti-inflammatory effects. Therefore, this study focuses on the isolation and identification of carotenoids from Meliloti herba and on the effect of isolated (all-E)-lutein 5,6-epoxide on primary sensory neurons and macrophages involved in nociception, as well as neurogenic and non-neurogenic inflammatory processes. The composition of the plant extracts was analyzed by high performance liquid chromatography (HPLC). The main carotenoid was isolated by column liquid chromatography (CLC) and identified by MS and NMR. The effect of water-soluble lutein 5,6-epoxide-RAMEB (randomly methylated-β-cyclodextrin) was investigated on Ca2+-influx in rat primary sensory neurons induced by the activation of the transient receptor potential ankyrin 1 receptor agonist to mustard-oil and on endotoxin-induced IL-1β release from isolated mouse peritoneal macrophages. (all-E)-Lutein 5,6-epoxide significantly decreased the percent of responsive primary sensory neurons compared to the vehicle-treated stimulated control. Furthermore, endotoxin-evoked IL-1β release from macrophages was significantly decreased by 100 µM lutein 5,6-epoxide compared to the vehicle-treated control. The water-soluble form of lutein 5,6-epoxide-RAMEB decreases the activation of primary sensory neurons and macrophages, which opens perspectives for its analgesic and anti-inflammatory applications.

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Isolation and identification of rumen bacteria capable of anaerobic rutin degradation

Canadian Journal of Microbiology ◽

10.1139/m69-247 ◽

1969 ◽

Vol 15 (12) ◽

pp. 1365-1371 ◽

Cited By ~ 39

Author(s):

K. -J. Cheng ◽

G. A. Jones ◽

F. J. Simpson ◽

M. P. Bryant

Keyword(s):

Volatile Fatty Acids ◽

Rumen Fluid ◽

Anaerobic Bacteria ◽

Heterocyclic Ring ◽

Ring Structure ◽

Water Soluble ◽

Rumen Bacteria ◽

Isolation And Identification ◽

Pure Cultures ◽

Rutin Degradation

Fifteen strains of bacteria capable of degrading rutin anaerobically were isolated from bovine rumen contents and identified by morphological and biochemical evidence as strains of Butyrivibrio sp. Three cultures from a laboratory collection of 53 strains of rumen bacteria also used rutin anaerobically. Two, Butyrivibrio fibrisolvens D1 and Selenomonas ruminantium GA192, cleaved the glycosidic bond of rutin and fermented the sugar but did not degrade the insoluble aglycone produced; the third strain, Peptostreptococcus sp. B178, degraded the substrate to soluble products. Butyrivibrio sp. C3 degraded rutin, quercitrin, and naringin to water-soluble products, showing that the organism cleaved the heterocyclic ring of these compounds. Butyrivibrio sp. C3 fermented the sugar moiety of hesperidin but did not cleave the heterocyclic ring. It did not attack quercetin, taxifolin, protocatechuic acid, or phloroglucinol. In a medium containing rumen fluid, Butyrivibrio sp. C3 degraded rutin more than twice as fast as it did in a medium containing enzymatic casein hydrolyzate, volatile fatty acids, yeast extract, and hemin in place of rumen fluid.The observations reported in this paper are believed to represent the first recorded demonstration of degradation of the heterocyclic ring structure of rutin and other bioflavonoids in pure cultures of anaerobic bacteria.

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Isolation and identification of seven metabolites of a water-soluble platelet aggregation inhibitor in rat urine

Xenobiotica ◽

10.3109/00498259509046680 ◽

1995 ◽

Vol 25 (11) ◽

pp. 1247-1257 ◽

Cited By ~ 3

Author(s):

M. Tanaka ◽

F. Ishikawa ◽

H. Hakusui

Keyword(s):

Platelet Aggregation ◽

Water Soluble ◽

Platelet Aggregation Inhibitor ◽

Isolation And Identification ◽

Rat Urine ◽

Aggregation Inhibitor

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Production, isolation and identification of low molecular mass peptides from blue cheese by high performance liquid chromatography

Journal of Dairy Research ◽

10.1017/s0022029900029940 ◽

1991 ◽

Vol 58 (3) ◽

pp. 363-372 ◽

Cited By ~ 35

Author(s):

Dolores Gonzalez de Llano ◽

M. Carmen Polo ◽

Mercedes Ramos

Keyword(s):

Amino Acids ◽

Molecular Mass ◽

High Performance ◽

Phosphotungstic Acid ◽

Soluble Fraction ◽

Water Soluble ◽

Isolation And Identification ◽

Blue Cheese ◽

Water Soluble Fraction ◽

Gel Permeation

SummaryThe blue cheese nitrogenous fractions soluble in water and in 5% phosphotungstic acid (PTA) were analysed by HPLC after 3–180 d ripening. In the water-soluble fraction, in addition to four or five major peaks corresponding to amino acids, there were many minor peaks, which increased during ripening. The low molecular mass peptides, soluble in 5% PTA, showed ripening-induced increments. A method combining precipitation with 5% PTA, gel permeation and subsequent HPLC was used to isolate some peptides of cheese. Four peptides containing between seven and ten residues were isolated and their amino acid composition and N-terminal residues determined.

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THE EXTRACELLULAR POLYSACCHARIDE OF GELATINOUS STRAINS OF CHROMOBACTERIUM VIOLACEUM

Canadian Journal of Microbiology ◽

10.1139/m60-017 ◽

1960 ◽

Vol 6 (2) ◽

pp. 153-163 ◽

Cited By ~ 7

Author(s):

William A. Corpe

Keyword(s):

Uronic Acid ◽

Extracellular Polysaccharide ◽

Amino Sugar ◽

Water Soluble ◽

Chromobacterium Violaceum ◽

Isolation And Identification ◽

Isolation And Purification ◽

Viscous Solution ◽

Hydrolysis Of

A method for the isolation and purification of the voluminous extracellular polysaccharide of Chromobacterium violaceum is presented. The purified product was fibrous when wet but dried into a tough, pliable film which was completely water soluble, forming a highly viscous solution. Hydrolysis of the polysaccharide, isolation and identification of the components established the presence of glucose as the principal sugar. A uronic acid and an amino sugar not conclusively identified were also present. Glucose, uronic acid, and the amino sugar were found in an approximate 5:1:1 ratio.

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Migration of water-soluble nitrogenous compounds of Feta cheese from the cheese blocks into the brine

International Dairy Journal ◽

10.1016/j.idairyj.2004.09.013 ◽

2005 ◽

Vol 15 (6-9) ◽

pp. 663-668 ◽

Cited By ~ 14

Author(s):

A.-M. Michaelidou ◽

E. Alichanidis ◽

A. Polychroniadou ◽

G. Zerfiridis

Keyword(s):

Water Soluble ◽

Nitrogenous Compounds ◽

Feta Cheese

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Studies on the Water-Soluble Constituents of Crude Drugs. VI. : Isolation and Identification of γ-Guanidinobutyric Acid and α, β-Diaminopropionic Acid from Semen Trichosanthes cucumeroides

YAKUGAKU ZASSHI ◽

10.1248/yakushi1947.88.4_488 ◽

1968 ◽

Vol 88 (4) ◽

pp. 488-489 ◽

Cited By ~ 4

Author(s):

TAKAO MURAKAMI ◽

NOBORU MORI ◽

MOTOO NAGASAWA

Keyword(s):

Water Soluble ◽

Isolation And Identification ◽

Crude Drugs ◽

Alpha Beta ◽

Diaminopropionic Acid

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Evaluation of Physiochemical Screening and Standardization on the Root of Rotula aquatica Lour.

Indian Journal of Pharmaceutical and Biological Research ◽

10.30750/ijpbr.1.1.6 ◽

2013 ◽

Vol 1 (01) ◽

pp. 63-67

Author(s):

Abhishek B. ◽

Avinash Saini

Keyword(s):

Moisture Content ◽

Water Soluble ◽

Isolation And Identification ◽

Crude Drug ◽

Successive Extraction ◽

The Family ◽

Microscopic Studies ◽

Crude Drugs ◽

Chloroform Methanol

The root of Rotula aquatica is also called as pashanbed, belonging to the family Borogenaceae. It is widely distributed in India from kumaun to Assam and western to southern India.The medicinal values of plant lie in their component phytochemicals such as alkaloids, flavonoids, phenolic compounds and other nutrients like as amino acid, proteins, which produce a definite physiological action on the human body. The proximate values such as moisture content, ash value, extractive value, were carried out. The dried roots powder was subjected to successive extraction with different solvents like petroleum ether, benzene, chloroform, methanol, ethanol and water. Macroscopic as well as microscopic studies of any crude drug are the primary steps to establish its botanical quality control before going to other studies. Hence Standardization of crude drug plays a very important role in identifying the purity and quality of crude drugs. The present investigation reveals Standardization which include Moisture Content, Total ash, Acid insoluble ash, Water soluble ash, Water soluble Extractive value, Alcohol soluble Extractive value, Phytochemical screening, and further isolation and identification of Phytoconstituents.

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