Estimation of additive and epistatic gene effects for phenotypic and biochemical traits in double hyploid lines of winter rape seed ,(Brassica napus L.)

2019 ◽  
Vol 79 (3) ◽  
Author(s):  
Jan Bocianowski ◽  
Kamila Nowosad ◽  
Agnieszka Dobrzycka ◽  
Joanna Wolko
Keyword(s):  
Brassica Napus ◽  
Genetic Parameters ◽  
Gene Interaction ◽  
Epistatic Effect ◽  
Brassica Napus L ◽  
Small Individual ◽  
Gene Effects ◽  
Epistatic Effects ◽  
Significant Additive Effect ◽  
Number Of Branches

In this paper 60 doubled haploid lines of winter oilseed rape (Brassica napus L.) were studied. Genetic parameters as additive and epistatic effects were estimated for 24 traits. The results indicate the importance of both these effects for number of branches per plant, number of siliques per plant, linoleic acid, total of glucosinolates, total of alkenyl glucosinolates, gluconapin, glucobrassicanapin, progoitryn, napoleiferin and indolyl in both years of this study. Statistically significant epistatic effect and non-significant additive effect for thousand seed weight means that this trait was determined by genes with small individual effects but strong gene by gene interaction effects. Confounding epistatic effects in models suggested that inheritance of this trait is complex and polygenic.

Screening rhizobacteria for improving the growth, yield, and oil content of canola (Brassica napus L.)

2004 ◽  
Vol 55 (2) ◽  
pp. 187 ◽  
Author(s):  
H. N. Asghar ◽  
Z. A. Zahir ◽  
M. Arshad
Keyword(s):  
Brassica Napus ◽  
Grain Yield ◽  
Root Length ◽  
Oil Content ◽  
Growth Promotion ◽  
Brassica Napus L ◽  
Growth Promoting ◽  
1000 Grain Weight ◽  
Number Of Branches

One hundred rhizobacteria previously isolated from the rhizospheres of Brassica species were screened for their growth promoting activity in Brassica napus L. under gnotobiotic conditions. Results revealed that 58% of the rhizobacteria increased root length (up to 139%), 39% enhanced shoot length (up to 78%), and shoot weight (up to 72%) of Brassica napus L. Based upon growth promotion of B. napus seedlings under gnotobiotic conditions, 10 promising plant-growth-promoting rhizobacteria (PGPR) were selected and tested for their effectiveness in growth promotion, yield, and oil content of B. napus grown in pots. The pot trials revealed that inoculation with selected PGPR increased plant height, root length, number of branches per plant, stem diameter, number of pods per plant, 1000-grain weight, grain yield, and oil content over a range of 7–57% above the uninoculated control. These isolates were then assayed for their ability to produce auxins in vitro in the presence and absence of L-tryptophan. Regression analysis showed that in vitro auxin production by these bacteria was significantly related to the number of branches and oil content of B. napus. It is highly likely that improvement in growth and yield of the inoculated plants is due to an increase in the number of branches per plant, since there was a positive correlation of this growth parameter with the number of pods per plant, 1000-grain weight, grain yield, and seed oil content. Results indicated that simultaneous screening of rhizobacteria for growth promotion under gnotobiotic conditions and in vitro production of auxins could be a useful approach for selecting effective PGPR.

scholarly journals Genetic Parameters and QTLs for Total Phenolic Content and Yield of Wheat Mapping Population of CSDH Lines under Drought Stress

2019 ◽  
Vol 20 (23) ◽  
pp. 6064 ◽  
Author(s):  
Czyczyło-Mysza ◽  
Cyganek ◽  
Dziurka ◽  
Quarrie ◽  
Skrzypek ◽  
...  
Keyword(s):  
Drought Stress ◽  
Total Phenolic Content ◽  
Genetic Parameters ◽  
Phenolic Content ◽  
Dry Weight ◽  
Total Phenolic ◽  
Doubled Haploid Population ◽  
Severe Drought ◽  
Gene Effects ◽  
Significant Additive Effect

A doubled haploid population of 94 lines from the Chinese Spring × SQ1 wheat cross (CSDH) was used to evaluate additive and epistatic gene action effects on total phenolic content, grain yield of the main stem, grain number per plant, thousand grain weight, and dry weight per plant at harvest based on phenotypic and genotypic observations of CSDH lines. These traits were evaluated under moderate and severe drought stress and compared with well-watered plants. Plants were grown in pots in an open-sided greenhouse. Genetic parameters, such as additive and epistatic effects, affecting total phenolic content, were estimated for eight year-by-drought combinations. Twenty-one markers showed a significant additive effect on total phenolic content in all eight year-by-drought combinations. These markers were located on chromosomes: 1A, 1B, 2A, 2B, 2D, 3A, 3B, 3D, 4A, and 4D. A region on 4AL with a stable QTL controlling the phenolic content, confirmed by various statistical methods is particularly noteworthy. In all years and treatments, three markers significantly linked to QTLs have been identified for both phenols and yield. Thirteen markers were coincident with candidate genes. Our results indicated the importance of both additive and epistatic gene effects on total phenolic content in eight year-by-drought combinations.

Triple test cross analysis in four sesame crosses (Sesamum indicum L.)

2001 ◽  
Vol 137 (2) ◽  
pp. 185-193 ◽  
Author(s):  
B. R. BAKHEIT ◽  
A. A. ISMAIL ◽  
A. A. EL-SHIEMY ◽  
F. S. SEDEK
Keyword(s):  
Plant Height ◽  
Seed Yield ◽  
Epistatic Effect ◽  
Gene Effects ◽  
Partial Dominance ◽  
Triple Test Cross ◽  
Test Cross ◽  
Triple Test ◽  
Oil Percentage ◽  
Number Of Branches

The present study was carried out during 1996, 1997 and 1998 to estimate the additive, dominance and epistatic components of genetic variation for yield, yield components and wilt infection by using 90 triple test cross families and their parents, F1 and F2 in each of four sesame crosses, i.e. Bengalian×Giza 32 (TTC1), Intro 259×Giza 25 (TTC2), Intro 413×Intro 777 (TTC3) and Shandaweel 5×Line 107 (TTC4). Mean squares from the genetic analysis of variance and the overall epistatic gene effects were highly significant differences for all studied characters. The [i] type (additive×additive) was the most important epistatic effect for height of the first capsule (TTC4), number of branches/plant (TTC1 and TTC3), length of the fruiting zone (TTC1, TTC2 and TTC3), days to the first flower and number of capsules/plant (TTC1, TTC2 and TTC4), 1000-seed (TTC3), wilt infection percentage (TTC3 and TTC4) and plant height, capsule length and seed yield/plant in all crosses. The ratio of (H/D)1/2 confirmed the presence of partial dominance for all studied traits. The highest proportion of recombinant lines was obtained for days to the first flower, number of branches/plant, height of the first capsule and seed yield/plant in TTC3 and plant height, capsule length, length of the fruiting zone number of capsules/plant, 1000-seed weight and oil percentage in TTC2.

scholarly journals The Rlm13 Gene, a New Player of Brassica napus–Leptosphaeria maculans Interaction Maps on Chromosome C03 in Canola

2021 ◽  
Vol 12 ◽  
Author(s):  
Harsh Raman ◽  
Rosy Raman ◽  
Yu Qiu ◽  
Yuanyuan Zhang ◽  
Jacqueline Batley ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Linkage ◽  
Leptosphaeria Maculans ◽  
Genotyping By Sequencing ◽  
Gene Interaction ◽  
Nucleotide Polymorphisms ◽  
Single Spore ◽  
Brassica Napus L ◽  
Single Spore Isolate ◽  
Trait Locus

Canola exhibits an extensive genetic variation for resistance to blackleg disease, caused by the fungal pathogen Leptosphaeria maculans. Despite the identification of several Avr effectors and R (race-specific) genes, specific interactions between Avr-R genes are not yet fully understood in the Brassica napus–L. maculans pathosystem. In this study, we investigated the genetic basis of resistance in an F2:3 population derived from Australian canola varieties CB-Telfer (Rlm4)/ATR-Cobbler (Rlm4) using a single-spore isolate of L. maculans, PHW1223. A genetic linkage map of the CB-Telfer/ATR-Cobbler population was constructed using 7,932 genotyping-by-sequencing-based DArTseq markers and subsequently utilized for linkage and haplotype analyses. Genetic linkage between DArTseq markers and resistance to PHW1223 isolate was also validated using the B. napus 60K Illumina Infinium array. Our results revealed that a major locus for resistance, designated as Rlm13, maps on chromosome C03. To date, no R gene for resistance to blackleg has been reported on the C subgenome in B. napus. Twenty-four candidate R genes were predicted to reside within the quantitative trait locus (QTL) region. We further resequenced both the parental lines of the mapping population (CB-Telfer and ATR-Cobbler, > 80 × coverage) and identified several structural sequence variants in the form of single-nucleotide polymorphisms (SNPs), insertions/deletions (InDels), and presence/absence variations (PAVs) near Rlm13. Comparative mapping revealed that Rlm13 is located within the homoeologous A03/C03 region in ancestral karyotype block “R” of Brassicaceae. Our results provide a “target” for further understanding the Avr–Rlm13 gene interaction as well as a valuable tool for increasing resistance to blackleg in canola germplasm.

A study of interaction of additive, dominance and epistatic gene effects with micro- and macro-environments in two tomato triple test crosses

1984 ◽  
Vol 103 (1) ◽  
pp. 53-57 ◽  
Author(s):  
R. P. Singh ◽  
S. Singh
Keyword(s):  
Flowering Time ◽  
Final Height ◽  
Gene Effects ◽  
Epistatic Effects ◽  
Triple Test ◽  
Maturity Period ◽  
Plant Yields ◽  
Number Of Branches

SummarySixty families of each of two tomato triple test crosses (S 120 × EC 61747 and EC 31513 × Pusa Ruby) were raised in completely randomized blocks in two replications with two fertilizer levels. Perkins & Jinks' (1971) analysis was used to detect and measure the interactions of additive, dominance and epistatic effects of genes with micro- and macro-environments for flowering time, maturity period, number of branches, final height, number of locules, number of fruits per plant, yields per plant and weight per fruit. Additive and dominance gene effects were almost equally sensitive to micro- and to macro-environments. The j and l type epistasis was more sensitive to the environments than the i type epistasis.

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