The Effect of Single Type Black Garlic (Allium Sativum L.) Extract on the Cell of Langerhans Islets and the Kidney Tubular Microscopy in Male Wistar Rats (Rattus Norvegicus) Models of Diabetes Mellitus

2020 ◽  
Vol 11 (06) ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Allium Sativum ◽  
Single Type ◽  
Langerhans Islets ◽  
Male Wistar Rats

scholarly journals EFEKTIFITAS EKSTRAK DAUN KEMBANG BULAN (Tithonia diversifolia) TERHADAP GAMBARAN HISTOPATOLOGI PANKREAS PADA TIKUS YANG DIINDUKSI ALOKSAN

2021 ◽  
Vol 11 (1) ◽  
pp. 15-24
Author(s):  
Ruswandono Wirjatmadja ◽  
Rondius Solfaine ◽  
Dian Ayu Kasrtikasari ◽  
Anisa Novia Wati
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Inflammatory Cells ◽  
Tithonia Diversifolia ◽  
The Moon ◽  
Male Wistar Rats ◽  
Made In

This study was conducted to determine the histopathological picture of the pancreas by induction of alloxan and extracts of the moon flower (tithonia diversifolia) in wistar rats (rattus norvegicus). A total of 24 male Wistar rats with 4 treatments were control P0, alloxan P1 with 1% CMC Na, P2 alloxan with 100mg / kgBB extract, alloxan P3 with 200mg / kgBB extract. Mice after said diabetes mellitus with a number> 135 mg / dL were then given treatment with 1% CMC Na on P1 and lunar leaf extract at P2 and P3, necropsy was carried out on the 15th day and the pancreatic organs were taken to be made in the form of preparations with coloring. HE, lesions observed Degeneration, Inflammatory Cells and Necrosis using a microscope, the results were analyzed using Kruskal wallis followed by Mann withney. The data obtained by degeneration in the kruskal wallis test were very significantly different because there were significant differences (P = 0.01), inflammation cells in the Kruskal wallis test were very significantly different because there were significant differences (P = 0.00), and for necrosis in the Kruskal wallis test significantly different because there are significant differences (0.02).

scholarly journals Effect of Caffeic Acid Phenethyl Ester Provision on Fibroblast Growth Factor-2, Matrix Metalloproteinase-9 Expression, Osteoclast and Osteoblast Numbers during Experimental Tooth Movement in Wistar Rats (Rattus norvegicus)

2021 ◽  
Author(s):  
Ida Bagus Narmada ◽  
Paristyawati Dwi Putri ◽  
Lucky Lucynda ◽  
Ari Triwardhani ◽  
I Gusti Aju Wahju Ardani ◽  
...  
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Movement ◽  
Caffeic Acid Phenethyl Ester ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Male Wistar Rats ◽  
Metalloproteinase 9

Abstract Objectives To investigate the effect of caffeic acid phenethyl ester (CAPE) provision on matrix metalloproteinase-9 (MMP-9), fibroblast growth factor-2 (FGF-2) expression, osteoclast and osteoblast numbers during experimental orthodontic tooth movement (OTM) in male Wistar rats (Rattus norvegicus). Materials and Methods Forty-eight healthy male Wistar rats (R. norvegicus), 16 to 20 weeks old with 200 to 250 g body weight (bw) were divided into several groups as follows: K1: OTM for 3 days; K2: OTM for 7 days; K3: OTM for 14 days; KP1: OTM and CAPE for 3 days; KP2: OTM and CAPE for 7 days; and KP3: OTM and CAPE for 14 days. A nickel titanium closed coil spring 8.0 mm long with 10 g/mm2 was installed between the upper left first molar and upper central incisor to move molar mesially. CAPE provision with a dose of 20 mg/kg bw of animal studies was done per orally. Immunohistochemistry was done to examine MMP-9 expression and osteoclast number in compression side as well as FGF-2 expression and osteoblast number in tensile side of the OTM. Statistical Analysis One-way analysis of variance test and Tukey’s honest significant difference test were performed to determine the difference between the groups (p < 0.05). Results MMP-9 expression and osteoclast numbers in the compression side were significantly different between the groups. Similarly, FGF-2 expression and osteoclast numbers in the tensile side were significantly different between the groups. Conclusions CAPE provision during OTM increases the number of osteoblasts and the FGF-2 expression significantly in the tensile side. Osteoclast numbers and MMP-9 expression significantly decrease in the compression side.

scholarly journals Increase of collagen in diabetes-related traumatic ulcers after the application of liquid smoke coconut shell

2017 ◽  
Vol 50 (2) ◽  
pp. 71
Author(s):  
Meircurius Dwi Condro Surboyo ◽  
Ira Arundina ◽  
Retno Pudji Rahayu
Keyword(s):  
Diabetes Mellitus ◽  
Topical Application ◽  
Wistar Rats ◽  
Healing Process ◽  
Coconut Shell ◽  
Diabetic Patients ◽  
Process Indicator ◽  
Liquid Smoke ◽  
Patients With Diabetes ◽  
Male Wistar Rats

Background: Traumatic ulcers in patients with diabetes mellitus may experience delayed healing despite their diabetic condition being controlled. Liquid smoke coconut shell containing phenolic compounds can potentially accelerate the healing process. One healing process indicator is the increased number of fibroblasts, another being the increased amount of collagen. Purpose: This study aimed to analyze the amount of collagen in traumatic ulcers in diabetics after application of liquid smoke coconut shell. Methods: Alloxan was induced in twenty-four male Wistar rats as models of diabetes mellitus. A traumatic 10 mm ulcer was made along the labial fornix incisive inferior with a round, stainless steel blade before liquid smoke coconut shell and benzydamine hydrochloride (as the control) was administrated once a day. A biopsy of the labial fornix incisive inferior was subsequently performed after the topical application for 5 and 7 days. Histological assessment was conducted to analyze the amount of collagen by means of Masson Trichome staining. Results: Histologically, the topical application of liquid smoke coconut shell for 5 days significantly increased the amount of collagen, higher than that of benzydamine hydrochloride as the control (p=0.006) (p<0.05). Meanwhile, the topical application of liquid smoke coconut shell for 7 days made the concentration of collagen no significantly different from that of benzydamine hydrochloride as the control (p=0.156) (p>0.05). Conclusion: Liquid smoke coconut shell applied for 5 days increase the amount of collagen in traumatic ulcers in diabetic patients.

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

Chronic administration of gabapentin and a gabapentin-carbamazepine combination reversibly suppress testicular function in male Wistar rats ( Rattus norvegicus )

2017 ◽  
Vol 24 (2) ◽  
pp. 63-69 ◽  
Author(s):  
Opeyemi Samson Osuntokun ◽  
Gbola Olayiwola ◽  
Ayowole Oladele ◽  
Idowu Ola ◽  
Oludele AyokaAbiodun
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Chronic Administration ◽  
Testicular Function ◽  
Male Wistar Rats

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals PENGARUH JAMU DENGAN Tribulus terrestris TERHADAP KUALITAS SPERMA TIKUS WISTAR JANTAN (Rattus norvegicus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Delano Pelealu ◽  
Lydia Tendean ◽  
Benny Wantouw
Keyword(s):  
Experimental Study ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Normal Morphology ◽  
Normal Sperm ◽  
Male Wistar Rats

Abstract: It is known that Tribulus terrestris can improve sperm quality. One of the Indonesian traditional medicine, jamu, contains this Tribulus terrestris. This study aimed to investigate the effect of jamu containing Tribulus terrestris on sperm quality which consisted of concentration, motility, and morphology of spermatozoa of male wistar rats (Rattus norvegicus). This was an experimental study. Nine male wistar rats were randomly divided into 3 groups of 3 rats each: Group P0 (control), group P1 (given 5 mg of Tribulus terrestris), and group P2 (given 10 mg of Tribulus terrestris). This study was conducted for 52 days. The results showed that the spermatozoa concentration of P1 was 49x106 sperm/ml suspension and of P2 53x106 sperm/ml suspension; both were lower than of P0 (59x106 sperm/ml suspension). The sperm motility category (A+B) of P1 was 33% and of P2 (37%); both were lower than of P0 (45%). The motility category A was not found in P1 and P2. The percentage of spermatozoa with normal morphology of P1 was 45% and the abnormal ones was 55%, meanwhile in P2 there was 49% of normal sperm morphology and 51% of abnormal morphology. Conclusion: Jamu containing Tribulus terrestris had no effect in improvement of the sperm quality.Keywords: jamu, Tribulus terrestris, sperm qualityAbstrak: Tribulus terrestris dikenal sebagai bahan yang dapat memperbaiki kualitas sperma. Salah satu jenis jamu yang diproduksi di Indonesia mengandung Tribulus terrestris Penelitian ini bertujuan untuk mengetahui pengaruh jamu dengan Tribulus terrestris terhadap konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar jantan (Rattus norvegicus). Penelitian ini menggunakan metode eksperimental. Sampel 9 ekor tikus wistar jantan (Rattus norvegicus) dibagi menjadi 3 kelompok yakni, kelompok P0 (kontrol); kelompok P1 (pemberian Tribulus terrestris 5 mg); dan kelompok P2 (pemberian Tribulus terrestris 10 mg). Penelitian dilakukan selama 52 hari. Hasil penelitian menunjukkan bahwa konsentrasi spermatozoa P1 49x106 sperma/ml suspensi dan P2 53x106 sperma/ml suspensi lebih rendah dibandingkan P0 59x106 sperma/ml suspensi. Motilitas spermatozoa kategori A+B pada P1 (33%) dan P2 (37%) lebih rendah dibandingkan pada P0 (45%). Tidak ditemukan motilitas kategori A pada P1 dan P2. Morfologi spermatozoa normal pada P1 sebesar 45% dan abnormal 55%. P2 memiliki morfologi spermatozoa normal sebesar 49% dan abnormal 51%. Simpulan: Pemberian jamu dengan Tribulus terrestris tidak meningkatkan kualitas sperma yang mencakup konsentrasi, motilitas, dan morfologi spermatozoa.Kata kunci: jamu, Tribulus terrestris, kualitas sperma

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