scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

scholarly journals The supplementation effect of Red Dragon fruit’s skin extract on the fasting blood glucose and lipid profiles in male Wistar rats with diabetes mellitus and dyslipidemia

2020 ◽  
Vol 3 (3) ◽  
pp. 87-92
Author(s):  
Stefani Marietta ◽  
AAG Budhiarta ◽  
I Wayan Weta
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Mean Difference ◽  
Control Group ◽  
Skin Extract ◽  
Before And After ◽  
Male Wistar Rats ◽  
Mean Differences

Background: Flavonoids, saponins, tannins, phenols, and vitamin-C contained in the Red Dragon fruit’s skin have a positive impact on glycemic control and lipid oxidation. This study aimed to determine the effect of Red Dragon fruit’s skin extract on reducing the fasting blood glucose (FBG) and improving the lipid profile of Wistar rats with diabetes and dyslipidemia. Methods: A randomized pre-test post-test control group experimental study was done on 22 male Wistar rats, aged 2-3 months that suffered from diabetes and dyslipidemia. Subjects were divided into the control group (given 2cc distilled water + 9 mg metformin) and the treatment group (given 160 mg red dragon fruit’s skin extract + 9 mg metformin) for 14 days. FBG and lipid profile measurements were done before and after the treatment. Data were analyzed using the compare mean test. Results: There was no significant mean difference of GDP between groups before (p=0.414) and after treatment (p=0.125), total cholesterol between groups before (p = 0.572) and after treatment (p=0.361), triglycerides between groups before (p=0.073) and after treatment (p=0.111). There was a significant mean difference of HDL between groups before (p=0.003) and after treatment (p=0.047), LDL between groups before (p=0.006) and after treatment (p=0.043). Although there were significant mean differences in HDL and LDL between groups before and after treatment, the pre-post treatment of HDL and LDL mean differences showed no significant mean difference (p=0.328 and p=0.704 consecutively). Conclusion: Red Dragon fruit’s skin extract treatment did not significantly reduce the mean FBG and lipid profile levels.

scholarly journals Combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract increased pancreatic beta cells number but have no effect toward glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus)

2019 ◽  
Vol 3 (2) ◽  
pp. 33
Author(s):  
Meiyati Panambunan ◽  
Wimpie Pangkahila ◽  
Anak Agung Gede Budhiarta
Keyword(s):  
Treatment Group ◽  
Beta Cells ◽  
Combination Treatment ◽  
Rattus Norvegicus ◽  
Pancreatic Beta Cells ◽  
Wistar Rats ◽  
Glycated Albumin ◽  
Field Of View ◽  
Control Group ◽  
Male Wistar Rats

Introduction: Diabetes mellitus is a complex and progressive disease which often lead to several debilitating complications that partly caused by by free radicals which can be overcome with antioxidants. Bay leaves contain essential oils, tannins, flavonoids and terpenoids which have considerable antioxidant properties. Therefore, this study aims to determine the effect combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract toward pancreatic beta cells density and glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus). Methods: An experimental posttest only control group study was conducted using 36 albino male rats, aged 2-3 months, weighing 180-200 grams. The rats were divided into 2 groups (n= 18) with control group treated with 1.8mg/200g body weight vildagliptin and 2cc placebo (aquabidest) while the treatment group received 1.8mg/200g BW vildagliptin and 250 mg/200g BW bay leaves extract. All treatment lasted for 21 days. Results: The results showed that pancreatic beta cell counts in the treatment group  was significantly higher than the control group  (109.07 ± 20.47 cells/field of view vs 90.87 ± 13.91 cells/field of view; p<0.01). However, the levels of Glycated albumin between two groups were not significantly different (treatment vs control: 17.33 ± 4.51 vs 20.18 ± 4.57; p=0.068). Conclusion: This study suggested that combination treatment of vildagliptin and bay leaf extract increased pancreatic beta cells but did not reduce glycated albumin levels in diabetic male Wistar rats.

scholarly journals Pemberian Ekstrak Daun Gandarusa (Justicia gendarussa Burm. f.) Menurunkan Kadar F2-isoprostan Urin Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
DG Ika Akpriyanti ◽  
Wimpie Pangkahila ◽  
IGM Aman
Keyword(s):  
Treatment Group ◽  
Physical Training ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Oxygen Species ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

Abstract: Excessive physical activity increases the consumption of oxygen which can result in increased levels of reactive oxygen species (ROS) causing oxidative stress characterized by the elevation of F2-isoprostane as a byproduct of lipid peroxidation. Willow (Justicia gendarussa Burm. f.) has an antioxidant activity that is able to inhibit and break the chain reaction of free radicals and prevent lipid peroxidation. This study was aimed to prove that willow leaf extract could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training. This was a true experimental study using 16 male Wistar rats (Rattus norvegicus), aged 2-3 months, weighing 180-200 g as subjects. The control group (P0) was treated with a placebo and excessive physical training, and the treatment group (P1) was treated with the willow leaf extract of 200mg/rat/day and excessive physical training. Before and after 14 days of treatment, the urine samples were collected and their levels of F2-isoprostane 8-isoPGF2α were examined by using enzyme immunoassay kit (EIA). The results showed that the mean level of F2-isoprostane in the control group (P0) before treatment (pretest) was 5.20±0.483 ng / mL while in the treatment group (P1) was 5.46±0.655 ng / mL (P > 0.05). On the other hand, after treatment for 7 days (post-test), the mean level of F2-isoprostane in the control group (P0) was 5.34±0.476 ng/mL and in the treatment group (P1) was 3.61±0.389 ng/mL (P < 0.01). Conclusion: The extract of willow (Justicia gendarussa Burm. f.) leaf could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training.Keywords: willow leaf, F2-isoprostane, excessive physical trainingAbstrak: Aktivitas fisik berlebih akan meningkatkan komsumsi oksigen yang dapat mengakibatkan peningkatan kadar reactive oxygen species (ROS) sehingga menyebabkan stres oksidatif yang ditandai dengan meningkatnya F2-isoprostan sebagai salah satu produk peroksidasi lipid. Tanaman gandarusa (Justicia gendarussa Burm. f.) mengandung antioksidan yang dapat mencegah reaksi peroksidasi lipid. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun gandarusa dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih. Jenis penelitian ialah eksperimental dengan subjek 16 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 6 bulan, berat badan 180-200 gr, dengan kadar F2-isoprostan >2ng/mL. Kelompok kontrol (P0) diberikan pelatihan fisik berlebih dan plasebo aquadest 2ml sedangkan kelompok perlakuan (P1) diberikan pelatihan fisik berlebih dan ekstrak daun gandarusa dosis 200mg/ekor/hari. Sebelum dan setelah 14 hari perlakuan, sampel urin ditampung dan diperiksa kadar F2-isoprostan menggunakan 8-isoPGF2α enzyme immunoassay kit (EIA) dari assay design untuk data pretest dan post-test. Hasil penelitian menunjukkan rerata kadar F2-isoprostan pada kelompok P0 sebelum perlakuan (pretest) ialah 5,20±0,483 ng/mL, sedangkan pada kelompokP1 ialah 5,46±0,655 ng/mL (P > 0,05). Setelah perlakuan selama 14 hari (post-test), rerata kadar F2-isoprostan pada kelompok P0 ialah 5,34±0,476 ng/mL dan pada kelompok P1 ialah 3,61±0,389 ng/mL (P < 0,01). Simpulan: Ekstrak daun gandarusa (Justicia gendarussa burm. f.) dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih.Kata kunci: daun gandarusa, F2-isoprostan, pelatihan fisik berlebih

scholarly journals The Effect of Chlorella vulgaris on Lipid Profile Wistar Strain Rats (Rattus norvegicus Berkenhout, 1769) Under Induced Stress

2019 ◽  
Vol 7 (1) ◽  
pp. 44
Author(s):  
Farah Nadia Karima ◽  
Mulyati Sarto
Keyword(s):  
Lipid Profile ◽  
Total Cholesterol ◽  
Chlorella Vulgaris ◽  
Rattus Norvegicus ◽  
Blood Lipid ◽  
Control Group ◽  
Stress Induction ◽  
Cholesterol Levels ◽  
Induced Stress ◽  
Wistar Strain

Stress is a psychiatric/psychological disorder characterized by a sense of disability, despair, anhedonia, decreased activity, and pessimism. Stress can affect the physiological condition of the body with symptoms of lipid metabolism disorders. Chlorella vulgaris is a microalgae that is known to have the potential as an alternative antidepressant drug. The study was conducted to determine the lipid profile of blood wistar strain rats after stress induction and the effect of administration of Chlorella vulgaris on blood lipid profiles of stress-induced wistar rats. This research is an experimental study using 5 treatments with 25 models of Rattus norvegicus wistar strain, 2 months old. The treatment group consisted of 1 control group, 1 stress group, and 3 groups of treatment variations, namely the treatment of antidepressant medication, the treatment of administration of cultivated Chlorella vulgaris, and the treatment of commercial Chlorella. Stress induction is carried out by treatments that given to the rats randomly, namely cold water, warm water, wet cage, dark-light cycle, and sound wave exposure for 40 days. Examination of blood lipid profiles was carried out on Day 0 after mice were acclimated, day 40 after rats were induced stress, and day 56 after rats were given treatment of cultivated Chlorella vulgaris. The results obtained were the stress conditions of the lipid profile of the wistar strain of rats which had increased were total cholesterol levels of the control group and triglyceride levels in all groups while those who had decreased were cholesterol levels other than the control group, HDL levels in all groups, and LDL levels all groups. Giving Chlorella vulgaris had effect on decreasing total cholesterol levels, triglyceride levels, and LDL levels along with increasing HDL levels.

scholarly journals Oral administration of Bali Robusta coffee (Coffea canephora) extract prevented the reduction of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training

2021 ◽  
Vol 4 (1) ◽  
pp. 37-41
Author(s):  
Kandy Rosa Ismalia ◽  
Wimpie Pangkahila ◽  
Ni Putu Sriwidyani
Keyword(s):  
Treatment Group ◽  
Physical Training ◽  
Rattus Norvegicus ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Coffea Canephora ◽  
Control Group ◽  
Testosterone Levels ◽  
Robusta Coffee ◽  
Male Wistar Rats

Background: Excessive exercise cause a deterioration of organ function and structure. Overtraining will cause reduce antioxidant reserve and excess free radical production. In this condition, there will be a decrease in the number of Leydig cells in the testis and followed by a decrease in testosterone level. Bali robusta coffee extract contains active compounds such as alkaloids, saponins, flavonoids, tannins and polyphenols. This study aims to prove whether Bali robusta coffee extract (Coffea canephora) can prevent a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training. Methods: Thirty-six rats were randomly divided into 2 groups: the control group (placebo aquabidest 2 ml) and the treatment group (Bali robusta coffee extract of 1 gram/kg BW). Both groups received excessive physical training in the form of swimming until the subjects experienced fatigue for 21-days. Results: The results showed that the mean of Leydig cell number in the treatment group was significantly higher, which was 3.70 ± 0.89 cell/field of view, compared to the control group, which was 2.92 ± 0.65 cell/field of view (p = 0.005). The mean testosterone level in the treatment group was also significantly higher, which was 6.32 ± 0.21 nmol/ml, compared to the control group, which was 1.95 ± 0.32 nmol/ml (p < 0.001).     Conclusion: The administration of Bali robusta coffee extract (Coffea canephora) prevented a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training.

scholarly journals Pemberian resveratrol oral mencegah peningkatan F2-Isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang dipapar tartrazine

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Yulyani . ◽  
Wimpie Pangkahila ◽  
Ferbian M. Siswanto
Keyword(s):  
Oral Administration ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Before And After ◽  
Male Wistar Rats ◽  
Immuno Assay ◽  
Enzyme Immuno Assay

Abstract: This study was aimed to prove that oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus). This was an experimental study using the pretest-posttest control group design. Subjects were 24 rats (Rattus norvegicus), Wistar strain, healthy, 2-3 months old, weighing 200-220 g, divided into 2 groups with 12 rats each. The first group (P0), the control group, was given a placebo of 2 ml distilled water 2 hours prior to the administration of tartrazine 75 mg/kg body weight. The second group (P1), the treatment group, was given resveratrol of 20 mg/kg 2 hour prior to the administration of tartrazine 75 mg/kg. Rats’ urine was collected before and after treatment for 4 weeks. Level of F2-isoprostane was examined by using an 8-iso-PGF2α enzyme immuno assay kit. The comparative analysis of the pretest groups showed that there was no difference between the average levels of F2-isoprostane in both groups (5.45±0.62 ng/mL in P0 group vs 5.42±0.64 ng/mL in P1 group) (P > 0.05). Meanwhile, after treatment for 4 weeks, the average level of F2-isoprostane in the P0 group was significantly different from the P1 group (6.61±0.93 ng/mL vs 3.79±0.48 ng/mL) (P < 0.01). Analysis of the treatment effect showed a significant increase of F2-isoprostane level in the P0 group, and a significant decrease in the P1 group (P < 0.01). Conclusion: Oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus).Keywords: tartrazine, resveratrol, F2-isoprostaneAbstrak: Tujuan penelitian untuk membuktikan pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan dalam urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine. Jenis penelitian eksperimental dengan menggunakan pretest-posttest control group design. Subyek penelitian ialah 24 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 2-3 bulan, dengan berat badan 200-220 gr, dibagi menjadi 2 kelompok, masing-masing berjumlah 12 ekor tikus. Kelompok pertama (P0) ialah kelompok kontrol, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan plasebo berupa aquadest 2 ml. Kelompok kedua (P1) ialah kelompok perlakuan, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan resveratrol 20 mg/kg BB. Saat sebelum dan sesudah perlakuan selama 4 minggu, urin tikus dikoleksi untuk pemeriksaan kadar F2-isoprostan menggunakan 8-iso-PGF2α enzyme immuno assay kit. Analisis komparasi sebelum perlakuan (pretest) menunjukkan rerata kadar F2-isoprostan pada kedua kelompok tidak berbeda nyata (5,45±0,62 ng/mL vs 5,42±0,64 ng/mL) (P > 0,05). Setelah perlakuan selama 4 minggu, rerata kadar F2-isoprostan pada kelompok P0 berbeda nyata dibandingkan kelompok P1 (6,61±0,93 ng/mL vs 3,79±0,48 ng/mL) (P < 0,01). Analisis efek perlakuan menunjukkan terjadi peningkatan kadar F2-isoprostan pada kelompok P0 dan penurunan bermakna pada kelompok P1 (P < 0,01). Simpulan: Pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine.Kata kunci: tartrazine, resveratrol, F2-isoprostan, urin

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

scholarly journals Efek ekstrak daun sirsak (Annona muricata Linn) terhadap profil lipid tikus putih jantan (Rattus Norvegicus)

2014 ◽  
Vol 3 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Indrawati Wurdianing ◽  
SA Nugraheni ◽  
Zen Rahfiludin
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Ldl Cholesterol ◽  
Total Cholesterol Level ◽  
Wilcoxon Test ◽  
Control Group ◽  
Annona Muricata ◽  
The Mean

Background: Lipid profile effects is a risk factor for Coronary Heart Disease. Soursop leaves (Annona muricata L) isa traditional medicine plant containing metabolic compounds that contribute to the improvement of the lipid profile.Objective: To determine the effects of soursop leaves extract on lipid profile (total cholesterol, LDL cholesterol, HDLcholesterol and triglyceride).Methods: An experimental study using randomized pre-posttest with control group design. Sample consisted of 28 maleWistar rats, were divided into four groups. The control group (K) was only given High Fat High Cholesterol (HFHC)diet and treatment groups (P1, P2, P3) were given a HFHC diet plus Annona muricata L extract with doses of 100, 200and 300 mg/kgBB per day for 28 days respectively. Data were analyzed by Wilcoxon test, Kruskal-Wallis and MannWhitney.Results: The mean total cholesterol level significantly decreased in the treatment group P1 (p = 0.028) from 60.7 mg/dl(47.6-75.3) to 45.5 mg/dl (38.4-62.4). Mean HDL cholesterol level significantly increased in the treatment group P2(p=0.043) from 26.0 mg/dl (19.7-35.3) to 27.9 mg/dl (18.8-38.0). The mean levels of LDL cholesterol and triglyceridedecreased but not significantly.Conclusion: The administration of Annona muricata L extract can decrease total cholesterol and increase HDLcholesterol significantly.

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals PENGARUH JAMU DENGAN Tribulus terrestris TERHADAP KUALITAS SPERMA TIKUS WISTAR JANTAN (Rattus norvegicus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Delano Pelealu ◽  
Lydia Tendean ◽  
Benny Wantouw
Keyword(s):  
Experimental Study ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Normal Morphology ◽  
Normal Sperm ◽  
Male Wistar Rats

Abstract: It is known that Tribulus terrestris can improve sperm quality. One of the Indonesian traditional medicine, jamu, contains this Tribulus terrestris. This study aimed to investigate the effect of jamu containing Tribulus terrestris on sperm quality which consisted of concentration, motility, and morphology of spermatozoa of male wistar rats (Rattus norvegicus). This was an experimental study. Nine male wistar rats were randomly divided into 3 groups of 3 rats each: Group P0 (control), group P1 (given 5 mg of Tribulus terrestris), and group P2 (given 10 mg of Tribulus terrestris). This study was conducted for 52 days. The results showed that the spermatozoa concentration of P1 was 49x106 sperm/ml suspension and of P2 53x106 sperm/ml suspension; both were lower than of P0 (59x106 sperm/ml suspension). The sperm motility category (A+B) of P1 was 33% and of P2 (37%); both were lower than of P0 (45%). The motility category A was not found in P1 and P2. The percentage of spermatozoa with normal morphology of P1 was 45% and the abnormal ones was 55%, meanwhile in P2 there was 49% of normal sperm morphology and 51% of abnormal morphology. Conclusion: Jamu containing Tribulus terrestris had no effect in improvement of the sperm quality.Keywords: jamu, Tribulus terrestris, sperm qualityAbstrak: Tribulus terrestris dikenal sebagai bahan yang dapat memperbaiki kualitas sperma. Salah satu jenis jamu yang diproduksi di Indonesia mengandung Tribulus terrestris Penelitian ini bertujuan untuk mengetahui pengaruh jamu dengan Tribulus terrestris terhadap konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar jantan (Rattus norvegicus). Penelitian ini menggunakan metode eksperimental. Sampel 9 ekor tikus wistar jantan (Rattus norvegicus) dibagi menjadi 3 kelompok yakni, kelompok P0 (kontrol); kelompok P1 (pemberian Tribulus terrestris 5 mg); dan kelompok P2 (pemberian Tribulus terrestris 10 mg). Penelitian dilakukan selama 52 hari. Hasil penelitian menunjukkan bahwa konsentrasi spermatozoa P1 49x106 sperma/ml suspensi dan P2 53x106 sperma/ml suspensi lebih rendah dibandingkan P0 59x106 sperma/ml suspensi. Motilitas spermatozoa kategori A+B pada P1 (33%) dan P2 (37%) lebih rendah dibandingkan pada P0 (45%). Tidak ditemukan motilitas kategori A pada P1 dan P2. Morfologi spermatozoa normal pada P1 sebesar 45% dan abnormal 55%. P2 memiliki morfologi spermatozoa normal sebesar 49% dan abnormal 51%. Simpulan: Pemberian jamu dengan Tribulus terrestris tidak meningkatkan kualitas sperma yang mencakup konsentrasi, motilitas, dan morfologi spermatozoa.Kata kunci: jamu, Tribulus terrestris, kualitas sperma

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