scholarly journals State of antioxidant protection system in carp hepatopancrease affected by associative Aeromonas Hydrophila infection with Saprolegnia Sp.

2020 ◽  
pp. 85-89
Author(s):  
Kh. Ya. Solopova ◽  
◽  
O. I. Vishchur ◽  
M. Z. Korylyak ◽  
I. Ye. Solovodzinska ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Aeromonas Hydrophila ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Defense System ◽  
Peroxidation Processes ◽  
Experimental Group

Bacterial diseases are responsible for heavy mortality in both wild and cultured fish. Co-infections are very common in nature and occur when hosts are infected by two or more different pathogens either by simultaneous or secondary infections so that two or more infectious agents are active together in the same host. Associative infections pose a significant threat to fish farming, so it is necessary to develop effective means of prevention and treatment of these infections; it is advisable to study in detail their impact on various fish. The aim of the study was to determine the intensity of lipid peroxidation processes and the activity of the antioxidant defense system in the body of carp suffering from aeromonosis and the associated infection of aeromonosis with saprolegniosis. The research was conducted at the Lviv Research Station of the Institute of Fisheries of NAAS. There were three groups of fish, 5 in each. The control group consisted of clinically healthy fish, the first experimental group (D1) – carps affected by Aeromonas hydrophila infection, the second experimental group (D2), which consisted of carps affected by associative Aeromonas hydrophila infection with Saprolegnia Sp. The results of studies have shown that carp with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. leads to the activation of lipid peroxidation processes, especially the primary product of LPO. Thus, in the hepatopancreas of fish there was an increase of 1.4 (p <0.05) in the reasonable content of diene conjugates and there was a tendency to increase the content of TBA-active products. At the same time, the inhibitory effect of pathogenic factors of this disease on the activity of the enzymatic link of the antioxidant defense system was stated. In particular, carp patients with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. had higher catalase and superoxide dismutase activity, respectively, 1.3 (p <0.05) and 2.3 (p <0.01) times. The results of experimental studies made it possible to expand and deepen the mechanisms of the pathogenesis of the co-infection of Aeromonas hydrophila infection with Saprolegnia Sp. and to use the data to develop effective treatments.

scholarly journals Antioxidant status of piglets under nitrate-nitrite load and the action of corrective factors

2020 ◽  
Vol 3 (2) ◽  
pp. 53-59
Author(s):  
K. Y. Leskiv ◽  
B. V. Gutyj ◽  
D. F. Gufriy ◽  
V. I. Khalak ◽  
O. G. Demchuk
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Diene Conjugates ◽  
Peroxidation Processes

The article presents the results of research on the state of the antioxidant defense system of piglets under nitrate-nitrite toxicosis and the action of corrective factors (methionine, phenarone and methyphene). Nitrate-nitrite load in piglets caused inhibition of the activity of enzymes of the antioxidant defense system, that indicates a decrease in the activity of superoxide dismutase, catalase and glutathione peroxidase in the serum of testing animals. The lowest activity of antioxidant enzymes under chronic nitrate-nitrite toxicosis was on the 60th day of the experiment in the serum of piglets of the control group, where accordingly the activity of superoxide dismutase decreased by 14 %, catalase activity – by 18 %, glutathione peroxidase activity – by 12 %. Lipid peroxidation processes are activated in the blood of animals, namely the level of lipid hydroperoxides and diene conjugates increases under the conditions of experimental nitrate-nitrite load. It should be noted that the highest level of lipid peroxidation products in the serum of piglets with chronic nitrate-nitrite toxicosis was on the 60 th day of the testing, and accordingly the content of lipid hydroperoxides increased by 67 % and diene conjugates – by 33 %. Administration of methionine, phenarone and methyphene under nitrate load contributed to the activation of the antioxidant defense system compared to animals in the control group. The use of antioxidant drugs in sick piglets also contributed to the inhibition of lipid peroxidation, as indicated by a decrease in the content of lipid hydroperoxides and diene conjugates in the serum of experimental group. More pronounced antioxidant effect was shown by administrating methyphene. The mechanism of action of phenarone and methyphene is associated with the direct effect of its components on the inhibition of the absorption of metabolites that have a toxic effect on cell membranes of animals. As a result of the adsorbing action of zeolite, which is part of the drugs, in the digestive tract there is a decrease in the concentration of substances that can be substrates for lipid peroxidation, as well as removal of toxic metabolites in the blood, which are prooxidants. This process occurs by osmosis and diffusion of these substances through the capillaries of the microvilli of the small intestine and their subsequent fixation on the sorbent granules.

scholarly journals The effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy

2020 ◽  
Vol 22 (100) ◽  
pp. 36-40
Author(s):  
I. S. Varkholiak ◽  
B. V. Gutyj ◽  
V. I. Kushnir ◽  
N. V. Nazaruk ◽  
O. I. Lisnyak ◽  
...  
Keyword(s):  
Heart Failure ◽  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Intraperitoneal Administration ◽  
Antioxidant Defense System ◽  
Feed Additives ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Experimental Group

Experimental studies obtained in recent years indicate the urgency of developing drugs of complex action to prevent the development of cardiovascular disease in dogs. This requires a much deeper study of the pathogenesis of heart failure, including cardiomyopathy. The aim of the study was the effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy. To reproduce heart failure in rats, an experimental model was used by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks. The study was performed on white sexually mature young male Wistar rats weighing 180–200 g, which were kept on the standard diet of the institute vivarium of the State Research Control Institute of Veterinary Drugs and Feed Additives. For the study, three groups of rats of 6 animals in each were formed: control group – intact animals; experimental group R1, in which animals were simulated doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin; experimental group R2, in which animals after injection of doxorubicin, intragastrically administered the drug “Bendamine” at a dose of 20 mg/kg. According to studies, doxorubicin intoxication in rats enhances LPO processes, as indicated by an increased content of lipid hydroperoxides by 47.4 %, diene conjugates – by 21.4 % and TBA-active products – by 24.9 % for animals of the control group. Doxorubicin cardiomyopathy in rats has a decrease in the activity of antioxidant enzymes in the blood of animals. In particular, the activity of catalase decreases by 36.9 % (P ≤ 0.001), and the activity of SOD – by 20.3 % (P ≤ 0.001). In the study of the activity of the glutathione link of the antioxidant defense system, similar changes were found as in the study of the activity of catalase and SOD. The use of the drug “Bendamine” in the rats of the experimental group helped to inhibit the excessive formation of lipid peroxidation products and increase the activity of both enzymatic and non-enzymatic parts of the antioxidant system.

scholarly journals Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

2019 ◽  
Vol 4 (1) ◽  
pp. 14-18
Author(s):  
Nadezhda A. Kurashova ◽  
Ekaterina A. Kudeyarova ◽  
Ekaterina O. Kuznetsova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Antioxidant Protection ◽  
Defense System ◽  
Total Antioxidant ◽  
Peroxidation Processes

Background.Today infertile marriage is not only a serious medical, but also a socio-demographic and economic problem. Male factor contributes averagely to half of the cases of the disease in couples. Such factors as high levels of reactive oxygen species and oxidative stress have been reported to compromise the process of spermatogenesis and sperm function in men. Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality.Aims.To study the characteristics of the intensity of the processes of lipoperoxidation and antioxidant status in the ejaculate of men with different variants of spermograms.Materials and methods.We examined 69 men with primary infertility and 155 fertile men. The content of lipid peroxidation components and antioxidant protection was determined by spectrophotometric method.Results.The results of the study in men with infertility and asthenozoospermia showed decreased total antioxidant activity of sperm by 50 % and α-tocopherol by 52 %, and in men with infertility and oligozoospermia, decreased total antioxidant activity of sperm by 47 % and α-tocopherol by 41 %.Conclusions.The analysis indicates a change in the parameters of the system of lipid peroxidation – antioxidant defense system and confirms the development of oxidative stress in them. Depending on the pathological state of the ejaculate in men of reproductive age, lipid peroxidation processes have their own characteristics. In men with oligozoospermia, peroxidation processes occur more intensively. Activation of lipid peroxidation – antioxidant defense system processes can be both a consequence and a cause of various metabolic changes in the human body.

scholarly journals Characteristics of lipid peroxidation processes and factors of the antioxidant defense system in chronic atrophic gastritis and gastric cancer

2022 ◽  
Vol 20 (4) ◽  
pp. 63-70
Author(s):  
O. V. Smirnova ◽  
V. V. Tsukanov ◽  
A. A. Sinyakov ◽  
O. L. Moskalenko ◽  
N. G. Elmanova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gastric Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Defense System ◽  
Glutathione S Transferase

Background. The problem of gastric cancer remains unresolved throughout the world, while chronic atrophic gastritis (CAG) increases the likelihood of its development by 15 times. In the Russian Federation, the incidence of gastric cancer (GC) is among the highest, with it prevailing among males. One of the leading mechanisms in molecular pathology of membranes is lipid peroxidation (LPO). The severity of oxidative membrane damage depends on concomitant diseases, contributing to emergence and progression of pathological processes and development of cancer. Currently, the problem of LPO is unsolved in biological systems.The aim of this study was to investigate the state of LPO and antioxidant defense system in CAG and GC. Materials and methods. The parameters were studied in 45 patients with CAG and 50 patients with GC. The control group included 50 practically healthy volunteers without gastrointestinal complaints, who did not have changes in the gastric mucosa according to the fibroesophagogastroduodenoscopy (FEGDS) findings.Results. In patients with CAG, an increase in malondialdehyde, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase was found in the blood plasma compared with the control group. In patients with CAG, lipid peroxidation was activated, and the malondialdehyde level increased by 3.5 times relative to normal values. At the same time, the body fought against oxidative stress by increasing the activity of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase. All patients with GC showed pronounced oxidative stress in the blood plasma in the form of a 45-fold increase in malondialdehyde. The activity of the main antioxidant enzyme superoxide dismutase was reduced in GC. Catalase was activated, which indicated pronounced oxidative stress, significant damage to blood vessels, and massive cell death. Glutathione-related enzymes (glutathione S-transferase and glutathione peroxidase) and the antioxidant protein ceruloplasmin were activated, which also indicated significant oxidative stress and severe intoxication in patients with GC.Conclusion. Depending on the stage and type of cancer, an in-depth study of lipid peroxidation and factors of the antioxidant defense system can be used to correct therapy and prevent cancer and can serve as markers of progression and prognosis in gastric cancer. 

The state of lipid peroxidation processes and the antioxidant defense system in obstructive sleep breathing disorders

Therapy ◽  
2020 ◽  
Vol 5_2020 ◽  
pp. 102-106
Author(s):  
Miroshnichenko A.I. Miroshnichenko ◽  
Petrova A.A. Petrova ◽  
Ivanov K.M. Ivanov ◽  
Krasikov S.I. Krasikov ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The State ◽  
Defense System ◽  
Sleep Breathing Disorders ◽  
Breathing Disorders ◽  
Obstructive Sleep ◽  
Peroxidation Processes

scholarly journals Рarameters of Oxidative Stress in Women with Diffuse Mastopathy in the Menstrual Cycle Course

2020 ◽  
pp. 40-47
Author(s):  
Lyudmila A. Grebenkina ◽  
Marina A. Darenskaya ◽  
Elena V. Osipova ◽  
Evgeniya V. Galchenko ◽  
Anastasia S. Lyubochko ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Menstrual Cycle ◽  
Antioxidant Defense ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Breast Diseases ◽  
Control Group ◽  
Defense System ◽  
General Hypothesis ◽  
Conjugated Trienes

Background: Despite the active study of the main pathogenetic mechanisms of benign breast diseases, there is still insufficient information on the lipid peroxidation – antioxidant defense processes activity in women with diffuse mastopathy in the course of menstrual cycle. Such knowledge is necessary for the early detection and prevention of hyperplastic processes and for the development of pathogenically based antioxidant therapy. Research hypothesis: the parameters of the lipid peroxidation – antioxidant defense system in women with diffuse mastopathy vary depending on the period of the menstrual cycle. Objective: to assess the activity of lipid peroxidation and antioxidant system reactions in women with diffuse mastopathy during the menstrual cycle. Materials and Methodology: The study included 29 women: 12 healthy ones (mean age 29.6 ± 2.32 years) and 17 women with diffuse mastopathy (mean age 28.8 ± 3.2 years). All women underwent standard collection of anamnesis and clinical examinations. For all women laboratory tests were performed to determine the concentration of lipid peroxidation products and antioxidant defense components. The tests were conducted every 3 days – on days 1–3, 4–6, 7–9, 10–12, 13–15, 16–18, 19–21, 22–24, and on days 25–28. Spectrophotometric and fluorometric methods were used. Results: Patients with diffuse mastopathy showed an increase in double bonds on days 1–3 (by 1.3 times), days 7–9 (by 1.22 times), 19–21 (by 1.19 times), and on days 22–24 (by 1.53 times); in conjugated dienes – on days 1–3 (by 1.4 times), 4–6 (by 1.2 times), 7–9 (by 1.47 times), 16–18 (by 1.48 times; P = .02), and on days 19–21 (by 1.38 times); in ketodienes and conjugated trienes (during the whole cycle) – on days 1–3 (by 2.64 times), 4–6 (by 1.6 times), 7–9 (by 1.72 times), 10–12 (by 1.39 times), 13–15 (by 1.36 times), 16–18 (by 3.46 times), 19–21 (by 2 times), 22–24 (by 2.54 times), and on days 25–28 (by 3.1 times); in thiobarbituric acid reactants – on days 19–21 (by 1.36 times) and 22–24 (by 1.27 times) compared with the control group. Patients with diffuse mastopathy showed an increase in total antioxidant activity on days 10–12 (by 1.67 times) and 16–18 (by 1.5 times); a decrease in superoxide dismutase activity – on days 16–18 (by 1.09 times) and 25–28 (by 1.25 times), in oxidized glutathione levels – on days 16–18 (by 1.23 times), 22–24 (by 1.14 times), and days 25–28 (by 1.25 times); an increase in reduced glutathione – on days 1–3 (by 1.3 times); a decrease in retinol content – on days 1–3 (by 1.64 times), 4–6 (by 1.2 times; P = .044), 10–12 (by 1.36 times), 19–21 (by 1.24 times) and on days 25–28 (by 1.18 times), and in ascorbate content – on days 7–9 (by 1.11 times) and 10–12 (by 1.16 times). Conclusion: During our study, we revealed an imbalance of lipid peroxidation – antioxidant defense system. For normalizing the balance in lipid peroxidation – antioxidant defense system, patients with diffuse mastopathy in the luteal phase of the menstrual cycle are recommended to use antioxidant drugs. To prove the general hypothesis further clinical trials are warranted.

scholarly journals The activity of the enzyme link of the antioxidant defense system of cows organism in experimental fasciolosis

2021 ◽  
Vol 23 (101) ◽  
pp. 3-7
Author(s):  
D. V. Frejuk ◽  
V. V. Stybel
Keyword(s):  
Redox Reactions ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
European Convention ◽  
Control Group ◽  
Defense System ◽  
Free Radical Reactions ◽  
Reversible Redox ◽  
Other Substances ◽  
Experimental Group

The antioxidant defense system of the organism controls and inhibits all stages of free radical reactions, starting from their initiation and ending to the formation of hydroperoxides and TBA-active products. The main mechanism of control of these reactions is associated with the chain of reversible redox reactions of metal ions, glutathione, ascorbate, tocopherol and other substances, the value of which is especially important for the preservation of long-standing macromolecules of nucleic acids and proteins, some components of membranes. The purpose of the research was to investigate the activity of the enzyme link of the antioxidant defense system of cows organism in experimental fasciolosis. Twelve cows of 4–5 years of age, Black-Spotted breed, were selected for the experiments, from which 2 groups were formed, six animals in each. Animals in control group (C) were clinically healthy. Animals of experimental group (E) were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed – selection and keeping of analogue animals in groups. The cows' ration was balanced in terms of nutrients and minerals. All animal manipulations were carried out in accordance with the European Convention for the Protection of Vertebrate Animals, which are used for experimental and scientific purposes. According to the results of our research, in cows affected by fasciolosis invasion, catalase activity in their blood decreased by 21.5 %, and superoxide dismutase activity by 30 %. In the research of the glutathione link of the antioxidant defense system, it was found that the activity of both glutathione peroxidase and glutathione reductase is also reduced in experimental fasciolosis. Thus, on the 25th day of the experiment, a decrease in these enzymes by 26.6 and 20.5 % is set. Thus, with the development of fasciolosis in ruminants, the balance in the complex “Antioxidant system – Lipid peroxidation” is disturbed, which in turn leads to complications of the disease. In the future it is planned to investigate the state of the non-enzymatic part of the antioxidant defense system of cows organism with experimental fasciolosis.

Lipid peroxidation and antioxidant defense system in patients with kidney cancer

2021 ◽  
pp. 36-40
Author(s):  
A. V. Alyasova ◽  
K. N. Kontorshchikova ◽  
Z. V. Amoev ◽  
E. А. Farafontova ◽  
T. N. Gorshkova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Kidney Cancer ◽  
Antioxidant Defense ◽  
Free Radical Oxidation ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Peroxidation Processes

Purpose of the study: to assess the state of lipid peroxidation processes in patients with kidney cancer in relation to the clinical factors of the prognosis of the disease and against the background of surgical intervention.Material and methods. For the study, we used blood plasma samples from 300 patients with kidney cancer T1–4N 0–1M0–1, obtained before surgery and 8–9 days after surgery. Oxidative stress was assessed by the intensity of free radical processes by the method of induced biochemiluminescence on the BKhL-07 biochemiluminometer apparatus (Medozons Co., Russia), the activity of lipid peroxidation processes was assessed by the accumulation of initial products in the blood plasma – diene conjugates (DC), triene conjugates (TC) and fial Schiff bases (OSh) according to I. A. Volchegorsky et al. method. (1989). Statistical processing of the results was carried out using Statistica 12 and Microsoft Offie Excel 2010 programs.Results. In patients with kidney cancer, there was a decrease in the level of DC, an increase in OSh and the ratio of Osh / (DC + TC) compared with the control (p ≤ 0.05), against the background of depletion of the resources of the antioxidant defense system. The content of DC did not differ from the norm with the size of the primary tumor of the kidney pT1–pT2 and decreased (p ≤ 0.05) in the case of the presence of the tumor pT3–pT4. At the same time, there was an increase in the OSh content (p ≤ 0.05). In patients with thrombi in the inferior vena cava system, a decrease in the level of DC was observed (p ≤ 0.05), the OSh level did not differ from that of healthy individuals. In the postoperative period, there was an increase in the content of DC (p ≤ 0.05) and a decrease in the Osh / (DC + TC) coeffiient (p ≤ 0.05) compared with the initial level.Conclusion. Kidney cancer is accompanied by the activation of free radical oxidation processes against the background of depletion of the resources of the antioxidant defense system. Changes in the pro-antioxidant homeostasis are interrelated with the size of the primary tumor and the presence of a tumor thrombus. In the postoperative period, the activity of free radical oxidation processes in the patient’s body decreases.

MORPHOLOGICAL INDICATORS OF BLOOD, RESISTANCE OF THE ORGANISM AND MEAT PRODUCTIVITY OF PIGLET AT APPLICATION OF 20-HYDROXYEXDISONE

2020 ◽  
Vol 1 (3) ◽  
pp. 371-377
Author(s):  
A.G. Solovieva ◽  
◽  
K.T. Erimbetov ◽  
O.V. Obvintseva ◽  
◽  
...  
Keyword(s):  
Blood Plasma ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Natural Resistance ◽  
Defense System ◽  
Growing Period ◽  
Meat Productivity ◽  
Experimental Group ◽  
In Blood Plasma

The effectiveness of using 20-hydroxyecdysone in the diet of piglets during the growing period and its effect on the formation of natural resistance, the antioxidant defense system of the body and their meat productivity were studied. The introduction of 20-hydroxyecdysone into the diet at the rate of 30 mg per kg of feed positively influenced on the bactericidal and lysozyme activity of blood serum, which in the experimental group were higher by 12,9 and 14,0%, respectively, compared with the control. The activity of superoxide dismutase and catalase enzymes in blood plasma in piglets of the experimental group was statistically significantly higher compared to the control. On the contrary, the content of malondialdehyde in blood plasma was statistically significantly lower in piglets of the experimental group compared with the control (17,1%, P≤0,01). In piglets of the experimental group, in comparison with the control, the output of muscle tissue (by 5,3%, P≤0,01), the area of the “muscle eye” (34,5%, P≤0,05) was statistically significantly higher, with less adipose tissue outlet.

scholarly journals Influence of psychoemotIonal stress on the functIonal actIvIty of neutrophIlIc granulocytes and lIpId peroxIdatIon IntensIty In qualIfIed athletes In competItIve perIod

2016 ◽  
Vol 1 (5) ◽  
pp. 90-95
Author(s):  
Базарин ◽  
Kirill Bazarin ◽  
Савченко ◽  
Andrey Savchenko
Keyword(s):  
Lipid Peroxidation ◽  
Functional Activity ◽  
Antioxidant Defense ◽  
Emotional State ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Objective Data ◽  
Neutrophilic Granulocytes ◽  
Defense System ◽  
Situational Anxiety

The EEG can be used to obtain objective data on the level of situational anxiety in athletes. The dynamics of the psy-cho-emotional state of the athletes during the annual training-competitive macrocycle is characterized by the following features: in the preparatory and transition periods demonstrated by athletes level of anxiety and psycho-emotional stress is lower than in the control group. Competitive period is usually characterized by significant increase in anxiety. Competitive loads experienced by athletes on the background of low anxiety, lead to less pronounced decrease in func-tional activity of neutrophils and activity of the antioxidant defense system.

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