scholarly journals The activity of the enzyme link of the antioxidant defense system of cows organism in experimental fasciolosis

2021 ◽  
Vol 23 (101) ◽  
pp. 3-7
Author(s):  
D. V. Frejuk ◽  
V. V. Stybel
Keyword(s):  
Redox Reactions ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
European Convention ◽  
Control Group ◽  
Defense System ◽  
Free Radical Reactions ◽  
Reversible Redox ◽  
Other Substances ◽  
Experimental Group

The antioxidant defense system of the organism controls and inhibits all stages of free radical reactions, starting from their initiation and ending to the formation of hydroperoxides and TBA-active products. The main mechanism of control of these reactions is associated with the chain of reversible redox reactions of metal ions, glutathione, ascorbate, tocopherol and other substances, the value of which is especially important for the preservation of long-standing macromolecules of nucleic acids and proteins, some components of membranes. The purpose of the research was to investigate the activity of the enzyme link of the antioxidant defense system of cows organism in experimental fasciolosis. Twelve cows of 4–5 years of age, Black-Spotted breed, were selected for the experiments, from which 2 groups were formed, six animals in each. Animals in control group (C) were clinically healthy. Animals of experimental group (E) were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed – selection and keeping of analogue animals in groups. The cows' ration was balanced in terms of nutrients and minerals. All animal manipulations were carried out in accordance with the European Convention for the Protection of Vertebrate Animals, which are used for experimental and scientific purposes. According to the results of our research, in cows affected by fasciolosis invasion, catalase activity in their blood decreased by 21.5 %, and superoxide dismutase activity by 30 %. In the research of the glutathione link of the antioxidant defense system, it was found that the activity of both glutathione peroxidase and glutathione reductase is also reduced in experimental fasciolosis. Thus, on the 25th day of the experiment, a decrease in these enzymes by 26.6 and 20.5 % is set. Thus, with the development of fasciolosis in ruminants, the balance in the complex “Antioxidant system – Lipid peroxidation” is disturbed, which in turn leads to complications of the disease. In the future it is planned to investigate the state of the non-enzymatic part of the antioxidant defense system of cows organism with experimental fasciolosis.

scholarly journals State of antioxidant protection system in carp hepatopancrease affected by associative Aeromonas Hydrophila infection with Saprolegnia Sp.

2020 ◽  
pp. 85-89
Author(s):  
Kh. Ya. Solopova ◽  
◽  
O. I. Vishchur ◽  
M. Z. Korylyak ◽  
I. Ye. Solovodzinska ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Aeromonas Hydrophila ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Defense System ◽  
Peroxidation Processes ◽  
Experimental Group

Bacterial diseases are responsible for heavy mortality in both wild and cultured fish. Co-infections are very common in nature and occur when hosts are infected by two or more different pathogens either by simultaneous or secondary infections so that two or more infectious agents are active together in the same host. Associative infections pose a significant threat to fish farming, so it is necessary to develop effective means of prevention and treatment of these infections; it is advisable to study in detail their impact on various fish. The aim of the study was to determine the intensity of lipid peroxidation processes and the activity of the antioxidant defense system in the body of carp suffering from aeromonosis and the associated infection of aeromonosis with saprolegniosis. The research was conducted at the Lviv Research Station of the Institute of Fisheries of NAAS. There were three groups of fish, 5 in each. The control group consisted of clinically healthy fish, the first experimental group (D1) – carps affected by Aeromonas hydrophila infection, the second experimental group (D2), which consisted of carps affected by associative Aeromonas hydrophila infection with Saprolegnia Sp. The results of studies have shown that carp with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. leads to the activation of lipid peroxidation processes, especially the primary product of LPO. Thus, in the hepatopancreas of fish there was an increase of 1.4 (p <0.05) in the reasonable content of diene conjugates and there was a tendency to increase the content of TBA-active products. At the same time, the inhibitory effect of pathogenic factors of this disease on the activity of the enzymatic link of the antioxidant defense system was stated. In particular, carp patients with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. had higher catalase and superoxide dismutase activity, respectively, 1.3 (p <0.05) and 2.3 (p <0.01) times. The results of experimental studies made it possible to expand and deepen the mechanisms of the pathogenesis of the co-infection of Aeromonas hydrophila infection with Saprolegnia Sp. and to use the data to develop effective treatments.

scholarly journals The effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy

2020 ◽  
Vol 22 (100) ◽  
pp. 36-40
Author(s):  
I. S. Varkholiak ◽  
B. V. Gutyj ◽  
V. I. Kushnir ◽  
N. V. Nazaruk ◽  
O. I. Lisnyak ◽  
...  
Keyword(s):  
Heart Failure ◽  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Intraperitoneal Administration ◽  
Antioxidant Defense System ◽  
Feed Additives ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Experimental Group

Experimental studies obtained in recent years indicate the urgency of developing drugs of complex action to prevent the development of cardiovascular disease in dogs. This requires a much deeper study of the pathogenesis of heart failure, including cardiomyopathy. The aim of the study was the effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy. To reproduce heart failure in rats, an experimental model was used by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks. The study was performed on white sexually mature young male Wistar rats weighing 180–200 g, which were kept on the standard diet of the institute vivarium of the State Research Control Institute of Veterinary Drugs and Feed Additives. For the study, three groups of rats of 6 animals in each were formed: control group – intact animals; experimental group R1, in which animals were simulated doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin; experimental group R2, in which animals after injection of doxorubicin, intragastrically administered the drug “Bendamine” at a dose of 20 mg/kg. According to studies, doxorubicin intoxication in rats enhances LPO processes, as indicated by an increased content of lipid hydroperoxides by 47.4 %, diene conjugates – by 21.4 % and TBA-active products – by 24.9 % for animals of the control group. Doxorubicin cardiomyopathy in rats has a decrease in the activity of antioxidant enzymes in the blood of animals. In particular, the activity of catalase decreases by 36.9 % (P ≤ 0.001), and the activity of SOD – by 20.3 % (P ≤ 0.001). In the study of the activity of the glutathione link of the antioxidant defense system, similar changes were found as in the study of the activity of catalase and SOD. The use of the drug “Bendamine” in the rats of the experimental group helped to inhibit the excessive formation of lipid peroxidation products and increase the activity of both enzymatic and non-enzymatic parts of the antioxidant system.

scholarly journals Characteristics of lipid peroxidation processes and factors of the antioxidant defense system in chronic atrophic gastritis and gastric cancer

2022 ◽  
Vol 20 (4) ◽  
pp. 63-70
Author(s):  
O. V. Smirnova ◽  
V. V. Tsukanov ◽  
A. A. Sinyakov ◽  
O. L. Moskalenko ◽  
N. G. Elmanova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gastric Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Defense System ◽  
Glutathione S Transferase

Background. The problem of gastric cancer remains unresolved throughout the world, while chronic atrophic gastritis (CAG) increases the likelihood of its development by 15 times. In the Russian Federation, the incidence of gastric cancer (GC) is among the highest, with it prevailing among males. One of the leading mechanisms in molecular pathology of membranes is lipid peroxidation (LPO). The severity of oxidative membrane damage depends on concomitant diseases, contributing to emergence and progression of pathological processes and development of cancer. Currently, the problem of LPO is unsolved in biological systems.The aim of this study was to investigate the state of LPO and antioxidant defense system in CAG and GC. Materials and methods. The parameters were studied in 45 patients with CAG and 50 patients with GC. The control group included 50 practically healthy volunteers without gastrointestinal complaints, who did not have changes in the gastric mucosa according to the fibroesophagogastroduodenoscopy (FEGDS) findings.Results. In patients with CAG, an increase in malondialdehyde, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase was found in the blood plasma compared with the control group. In patients with CAG, lipid peroxidation was activated, and the malondialdehyde level increased by 3.5 times relative to normal values. At the same time, the body fought against oxidative stress by increasing the activity of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase. All patients with GC showed pronounced oxidative stress in the blood plasma in the form of a 45-fold increase in malondialdehyde. The activity of the main antioxidant enzyme superoxide dismutase was reduced in GC. Catalase was activated, which indicated pronounced oxidative stress, significant damage to blood vessels, and massive cell death. Glutathione-related enzymes (glutathione S-transferase and glutathione peroxidase) and the antioxidant protein ceruloplasmin were activated, which also indicated significant oxidative stress and severe intoxication in patients with GC.Conclusion. Depending on the stage and type of cancer, an in-depth study of lipid peroxidation and factors of the antioxidant defense system can be used to correct therapy and prevent cancer and can serve as markers of progression and prognosis in gastric cancer. 

scholarly journals Results of the use of a sorbent in a carp feed with signs of mycotoxin contamination

2021 ◽  
pp. 72-86
Author(s):  
О. Deren ◽  
◽  
N. Syrovatka ◽  
M. Koryliak ◽  
О. Dobryanska ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Physiological Parameters ◽  
Feed Additives ◽  
Control Group ◽  
Average Weight ◽  
Defense System ◽  
Diene Conjugates

Purpose. The aim of the work was to study certain morpho-physiological parameters of carp as a result of additional introduction of a mycotoxin sorbent to a feed with signs of spoilage. Methodology. During 2019–2020, two experiments were conducted in the laboratory. The first one included 7 groups-analogues of age-1+ Nyvky carp, 20 fish in each group, with an average weight of 22.5 g. The control group of fish was fed for 24 days with a compound feed with signs of spoilage, «Mykosorb» was additionally added to the diet of the experimental groups in the amounts of 0.05, 0.1; 0.15; 0.2; 0.25 and 0.4%, respectively. The second group included 4 groups of age-1+ fish, 12 fish in each group, with an average weight of 20.0 g. The diet of the control group was identical to that of the first experiment, and the experimental groups with the addition of 0.05, 0.075 and 0.1% of «Mycosorb», respectively. During the study, optimal conditions for keeping fish were provided. Fish after were measured and weighed at the end of the experiment and their weight gains were analyzed according to generally accepted methods in fish farming. The activity of antioxidant enzymes and the content of lipid peroxidation products depending on the composition of the diet were studied. Findings. The studies used compound feed with signs of spoilage as a result of violation of their storage technology, which identified three groups of toxins: Aflatoxin B1, Zearalenone and Deoxynivalenol. As a result of the first stage of the study, the rates of application of mycotoxin sorbent into the diet of carp at amounts of 0.05 - 0.2% were determined. At the same time, there was no clear proportional pattern of changes in the average weight of fish depending on the amount of introduction of «Mycosorb», the value increased in all experimental groups compared to the control by 0.4 – 15.2%. Muscles of age-1+ fish were found to have higher (p<0,001) catalase activity after application of 0.15% of the test drug by 1.4 times, 0.2% — by 46%, 0.25% — by 69 and 0.4% — by 79%. Superoxide dismutase activity also increased, except for the groups of 0.2, 0.25 and 0.4% of «Mycosorb». There was a tendency of 21.0 and 22.6% decrease in the content of diene conjugates as a result of feeding 0.05 and 0.1% sorbent and some increase as a result of feeding 0.15; 0.2; 0.25 and 0.4% additives. Studies of the next stage showed that addition of «Mykosorb» at the rate of 0.05, 0.075 and 0.1% to the feed, the average weight of age-1+ carp in the experimental groups increased by 45.4; 37.2 and 30.3%, the condition factor — by 41.9; 40.8 and 42.7%, the relative growth rate is 4.3; 5.9 and 4.6 times, respectively. The fish body height to length ratio was lower by 10.3, 7.0 and 7.4% relative to the control group. Addition of 0.05, 0.075 and 0.1% of «Mycosorb» increased resistance of fish due to an increase in the hepatopancreatic activity of antioxidant enzymes: superoxide dismutase by 28.3, 30.5 and 5.0% and catalase by 56.5 (p<0.001), 55.5 (p<0.001) and 46.3% (p<0.05). At the same time, addition of 0.05 and 0.1% of «Mykosorb» increased the content of diene conjugates by 11.3 (p<0.01) and 12.3% (p<0.01), TBC-active products by 19.7 (p<0.05) and 13.2%. Originality. For the first time, the feasibility of addition of the mycotoxin sorbent «Mykosorb» into the feed with signs of spoilage was studied. The effect of feeding age-1+ carp with diets of various compositions on some morpho-physiological parameters of carp in the conditions of model tests was carried out. Practical value. Additional weigh gains and improvements of the antioxidant defense system of the carp organism were observed. The use of «Mykosorb» in carp feeding allows reducing the negative impact of low quality feed on fish body. Key words: carp, feed, feed additives, mycotoxins, sorbent, morphological parameters, growth, antioxidant defense system

Salivary Antioxidant and Oxidative Stress Marker Levels in HIV-Positive Individuals

2019 ◽  
Vol 22 (1) ◽  
pp. 59-64
Author(s):  
Samaneh Vaziri Amjad ◽  
Poorandokht Davoodi ◽  
Mohammad Taghi Goodarzi ◽  
Hamidreza Abdolsamadi ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Oxidative Stress Marker ◽  
Hiv Positive ◽  
Control Group ◽  
Defense System ◽  
Positive Group ◽  
Total Antioxidant ◽  
And Oxidative Stress

Background:HIV infections are a worldwide health problem. HIV infection reduces CD4+ cell counts. Oxidative stress might play an important role in the stimulation of virus replication and immunodeficiency. Saliva might be the first line of defense against oxidative stress.Objective:The aim of this study was to evaluate the oxidative stress marker and antioxidant levels of saliva in HIV-infected patients by measuring total antioxidant capacity and malondialdehyde level.Methods:A total of 49 HIV-positive patients and 49 healthy HIV-negative individuals were randomly selected. All the patients were clinically examined. Five mL of unstimulated whole saliva was collected and evaluated by spectrophotometric assay. Data were analyzed with STATA 11.Results:Mean ages of the case and control groups were 28 and 33 years, respectively. Salivary malondialdehyde levels were significantly higher in the HIV-positive group (3.68±2.26) compared to the healthy control group (2.79±1.91). Levels of salivary total antioxidant capacity were significantly lower in the HIV-positive group (0.20± 0.09) compared to the control group (0.27±0.10).Conclusion:The antioxidant defense system in HIV-positive individuals was low and oxidative stress was high in this population. Saliva might be used as a diagnostic tool for antioxidant changes in HIV-positive patients in the future. There were changes in salivary antioxidant defense system and oxidative stress in HIV-positive individuals. Antioxidant supplements might help local salivary and general health statuses.

Curcumin protects the testis against cadmium-induced histopathological damages and oxidative stress in mice

2019 ◽  
Vol 39 (5) ◽  
pp. 653-661 ◽  
Author(s):  
HR Momeni ◽  
N Eskandari
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Enzymes ◽  
Adverse Effects ◽  
Cadmium Chloride ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Defense System ◽  
Total Glutathione

Background: Cadmium is an environmental pollutant which can induce the overproduction of free radicals while suppressing the antioxidant defense system. Curcumin is considered a free-radical scavenger and a potent antioxidant. This study was conducted to investigate the effect of curcumin on serum antioxidant enzymes and histopathological changes in mice treated with cadmium. Methods: In this experimental study, adult mice were divided into four groups, namely, control, cadmium chloride (5 mg kg−1), curcumin (100 mg kg−1), and curcumin+cadmium chloride. The animals received curcumin 24 h prior to cadmium chloride injection. After 24 h, blood samples were collected and used to assess the levels of malondialdehyde (MDA), antioxidant enzymes activity (catalase, superoxide dismutase, and glutathione peroxidase), total glutathione, total thiol, and hydrogen peroxide. Histopathological evaluation was also performed for testicular tissue. Results: Mice treated with cadmium showed a significant ( p < 0.001) decrease in the activity of antioxidant enzymes, serum amounts of total glutathione and total thiol, and the diameter of seminiferous tubules compared to the control group. This pollutant also significantly ( p < 0.001) increased serum levels of MDA and hydrogen peroxide and the lumen diameter of seminiferous tubules compared to the control group. In the curcumin+cadmium group, curcumin significantly ( p < 0.001) reversed the adverse effects of cadmium, compared to the cadmium group. In addition, curcumin alone significantly ( p < 0.001) increased serum glutathione peroxidase activity and thiol content compared to the control group. Conclusion: Curcumin, as a potent antioxidant, could compensate the adverse effects of cadmium on lipid and protein peroxidation, potentiated serum antioxidant defense system, and ameliorated some morphometrical parameters in the testis of cadmium-treated mice.

scholarly journals CONTRIBUTION OF PSYCHOEMOTIONAL STRESS TO THE DEVELOPMENT OF ADAPTATIONAL FAILURE IN THE COMPETITIVE PERIOD OF QUALIFIED ATHLETES

2016 ◽  
Vol 1 (2) ◽  
pp. 7-11 ◽  
Author(s):  
������� ◽  
Kirill Bazarin ◽  
�������� ◽  
Andrey Savchenko
Keyword(s):  
Emotional Stress ◽  
Functional Activity ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Objective Data ◽  
Defense System ◽  
Pronounced Decrease ◽  
Situational Anxiety ◽  
Psychoemotional State

The electroencephalography can be used to obtain objective data on the level of situational anxiety in athletes. The dynamics of the psychoemotional state of the athletes during the annual training-competitive macrocycle is characterized by the following features: in the preparatory and transition periods of the athletes the levels of anxiety and psycho-emotional stress are lower than in the control group. Competitive period is usually characterized by a significant increase in anxiety. Competitive loads experienced by athletes on the background of low anxiety lead to a less pronounced decrease in functional activity of neutrophils and activity of the antioxidant defense system.

Oophorectomy hinders antioxidant adaptation promoted by swimming in Wistar rats

2013 ◽  
Vol 38 (2) ◽  
pp. 148-153 ◽  
Author(s):  
Ulisvaldo Brunno de Oliveira Macedo ◽  
Rand Randall Martins ◽  
Francisco Paulo Freire Neto ◽  
Yonara Monique da Costa Oliveira ◽  
Aldo da Cunha Medeiros ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Wistar Rats ◽  
Antioxidant Defense ◽  
Hormone Replacement ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Defense System ◽  
Antioxidant Parameters ◽  
The Brain

Oxidative stress is associated with postmenopause and is also responsible for various metabolic alterations. The redox imbalance observed during ovarian decline can be induced experimentally by bilateral ovariectomy in rats. In addition to hormone replacement, regular moderate physical exercise is indicated to prevent several common postmenopausal diseases. This study aimed to assess the effect of daily swimming on the antioxidant defense system of oophorectomized Wistar rats. Control and oophorectomized groups were submitted to 1 h of daily swimming for 90 days. Levels of lipid peroxidation and glutathione content and the activities of superoxide dismutase enzyme and glutathione peroxidase in erythrocytes, liver, and brain were assessed every 30 days. The control group exhibited lower lipoperoxidation that was associated with a significant increase in superoxide dismutase enzyme activity, glutathione peroxidase activity, and glutathione content in erythrocytes and liver; however, swimming did not cause changes in antioxidant parameters in the brain over time. The oophorectomized group showed no antioxidant adaptation to daily swimming and had greater oxidative damage in the liver and blood. Our results suggest that ovariectomy hinders antioxidant adaptation in Wistar rats submitted to daily swimming.

scholarly journals Enzymatic response of Macrobrachium jelskii (Miers, 1877) exposed to water from urban and rural rivers in Bahia, Brazil

2021 ◽  
Vol 10 (6) ◽  
pp. e46010615638
Author(s):  
Thais Arrais Mota ◽  
Elissandra Ulbricht Winkaler ◽  
Guilherme de Oliveira ◽  
Sergio Schwarz da Rocha
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defense ◽  
Sampling Site ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Water Sampling ◽  
Defense System ◽  
Glutathione S Transferase ◽  
Rivers State ◽  
Dry And Rainy Seasons

Several biomarker enzymes such as catalase (CAT) and glutathione S-transferase (GST) can be used to measure oxidative stress in animals caused by exposure to xenobiotics. The objective of the present study was to characterize different points of the Capivari (CP1 and CP2), Paraguaçu (PG1 and PG2) and Subaé (SB1 and SB2) Rivers, state of Bahia, in relation to the presence of xenobiotics, using CAT and GST as bioindicators in M. jelskii. The water-sampling sites were considered urban or rural and in all of them signs of environmental degradation were observed. Therefore, acute exposure tests (96h) were performed with water samples collected during the dry and rainy seasons. Results showed that the activity of CAT and GST in prawns exposed to water from CP1 and CP2 were very similar, while those exposed to water from PG1, PG2, SB1 and SB2 formed distinct groups of data. Significant increase in the activity of at least one of the analyzed enzymes in each sampling site was observed, when compared to animals in the control group. This demonstrated a possible oxidative stress in M. jelskii caused by the presence of xenobiotics in the water (e.g., domestic sewage, pesticides, oil, and heavy metals). Enzymatic activities were higher in animals from experiments carried out in the rainy season, except for the CAT activity of animals exposed to water from Subaé River. This study demonstrated the potential of M. jelskii as bioindicator and contributed to the knowledge of aspects of the antioxidant defense system of this species.

scholarly journals Рarameters of Oxidative Stress in Women with Diffuse Mastopathy in the Menstrual Cycle Course

2020 ◽  
pp. 40-47
Author(s):  
Lyudmila A. Grebenkina ◽  
Marina A. Darenskaya ◽  
Elena V. Osipova ◽  
Evgeniya V. Galchenko ◽  
Anastasia S. Lyubochko ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Menstrual Cycle ◽  
Antioxidant Defense ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Breast Diseases ◽  
Control Group ◽  
Defense System ◽  
General Hypothesis ◽  
Conjugated Trienes

Background: Despite the active study of the main pathogenetic mechanisms of benign breast diseases, there is still insufficient information on the lipid peroxidation – antioxidant defense processes activity in women with diffuse mastopathy in the course of menstrual cycle. Such knowledge is necessary for the early detection and prevention of hyperplastic processes and for the development of pathogenically based antioxidant therapy. Research hypothesis: the parameters of the lipid peroxidation – antioxidant defense system in women with diffuse mastopathy vary depending on the period of the menstrual cycle. Objective: to assess the activity of lipid peroxidation and antioxidant system reactions in women with diffuse mastopathy during the menstrual cycle. Materials and Methodology: The study included 29 women: 12 healthy ones (mean age 29.6 ± 2.32 years) and 17 women with diffuse mastopathy (mean age 28.8 ± 3.2 years). All women underwent standard collection of anamnesis and clinical examinations. For all women laboratory tests were performed to determine the concentration of lipid peroxidation products and antioxidant defense components. The tests were conducted every 3 days – on days 1–3, 4–6, 7–9, 10–12, 13–15, 16–18, 19–21, 22–24, and on days 25–28. Spectrophotometric and fluorometric methods were used. Results: Patients with diffuse mastopathy showed an increase in double bonds on days 1–3 (by 1.3 times), days 7–9 (by 1.22 times), 19–21 (by 1.19 times), and on days 22–24 (by 1.53 times); in conjugated dienes – on days 1–3 (by 1.4 times), 4–6 (by 1.2 times), 7–9 (by 1.47 times), 16–18 (by 1.48 times; P = .02), and on days 19–21 (by 1.38 times); in ketodienes and conjugated trienes (during the whole cycle) – on days 1–3 (by 2.64 times), 4–6 (by 1.6 times), 7–9 (by 1.72 times), 10–12 (by 1.39 times), 13–15 (by 1.36 times), 16–18 (by 3.46 times), 19–21 (by 2 times), 22–24 (by 2.54 times), and on days 25–28 (by 3.1 times); in thiobarbituric acid reactants – on days 19–21 (by 1.36 times) and 22–24 (by 1.27 times) compared with the control group. Patients with diffuse mastopathy showed an increase in total antioxidant activity on days 10–12 (by 1.67 times) and 16–18 (by 1.5 times); a decrease in superoxide dismutase activity – on days 16–18 (by 1.09 times) and 25–28 (by 1.25 times), in oxidized glutathione levels – on days 16–18 (by 1.23 times), 22–24 (by 1.14 times), and days 25–28 (by 1.25 times); an increase in reduced glutathione – on days 1–3 (by 1.3 times); a decrease in retinol content – on days 1–3 (by 1.64 times), 4–6 (by 1.2 times; P = .044), 10–12 (by 1.36 times), 19–21 (by 1.24 times) and on days 25–28 (by 1.18 times), and in ascorbate content – on days 7–9 (by 1.11 times) and 10–12 (by 1.16 times). Conclusion: During our study, we revealed an imbalance of lipid peroxidation – antioxidant defense system. For normalizing the balance in lipid peroxidation – antioxidant defense system, patients with diffuse mastopathy in the luteal phase of the menstrual cycle are recommended to use antioxidant drugs. To prove the general hypothesis further clinical trials are warranted.

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