MORPHOLOGICAL INDICATORS OF BLOOD, RESISTANCE OF THE ORGANISM AND MEAT PRODUCTIVITY OF PIGLET AT APPLICATION OF 20-HYDROXYEXDISONE

2020 ◽  
Vol 1 (3) ◽  
pp. 371-377
Author(s):  
A.G. Solovieva ◽  
◽  
K.T. Erimbetov ◽  
O.V. Obvintseva ◽  
◽  
...  
Keyword(s):  
Blood Plasma ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Natural Resistance ◽  
Defense System ◽  
Growing Period ◽  
Meat Productivity ◽  
Experimental Group ◽  
In Blood Plasma

The effectiveness of using 20-hydroxyecdysone in the diet of piglets during the growing period and its effect on the formation of natural resistance, the antioxidant defense system of the body and their meat productivity were studied. The introduction of 20-hydroxyecdysone into the diet at the rate of 30 mg per kg of feed positively influenced on the bactericidal and lysozyme activity of blood serum, which in the experimental group were higher by 12,9 and 14,0%, respectively, compared with the control. The activity of superoxide dismutase and catalase enzymes in blood plasma in piglets of the experimental group was statistically significantly higher compared to the control. On the contrary, the content of malondialdehyde in blood plasma was statistically significantly lower in piglets of the experimental group compared with the control (17,1%, P≤0,01). In piglets of the experimental group, in comparison with the control, the output of muscle tissue (by 5,3%, P≤0,01), the area of the “muscle eye” (34,5%, P≤0,05) was statistically significantly higher, with less adipose tissue outlet.

State of antioxidant mechanisms in ischemic insults

1999 ◽  
Vol 80 (5) ◽  
pp. 371-373
Author(s):  
L. I. Reikhert
Keyword(s):  
Cerebrospinal Fluid ◽  
Blood Plasma ◽  
Antioxidant Defense ◽  
Clinical Manifestations ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Ischemic Insult ◽  
Antioxidant Mechanisms ◽  
In Blood Plasma

The activity of superoxidismutase and catalase in thrombocytes as well as content of -tocopherol and -carotin in blood plasma and cerebrospinal fluid in 193 patients with ischemic insult are studied. Incompetence of the mechanisms of antioxidant defense correlating with gravity and outcome of ischemic insult is established. Depression of various links of the antioxidant defense system in blood plasma, thrombocytes and cerebrospinal fluid affects the gravity of clinical manifestations of the disease.

scholarly journals State of antioxidant protection system in carp hepatopancrease affected by associative Aeromonas Hydrophila infection with Saprolegnia Sp.

2020 ◽  
pp. 85-89
Author(s):  
Kh. Ya. Solopova ◽  
◽  
O. I. Vishchur ◽  
M. Z. Korylyak ◽  
I. Ye. Solovodzinska ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Aeromonas Hydrophila ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Defense System ◽  
Peroxidation Processes ◽  
Experimental Group

Bacterial diseases are responsible for heavy mortality in both wild and cultured fish. Co-infections are very common in nature and occur when hosts are infected by two or more different pathogens either by simultaneous or secondary infections so that two or more infectious agents are active together in the same host. Associative infections pose a significant threat to fish farming, so it is necessary to develop effective means of prevention and treatment of these infections; it is advisable to study in detail their impact on various fish. The aim of the study was to determine the intensity of lipid peroxidation processes and the activity of the antioxidant defense system in the body of carp suffering from aeromonosis and the associated infection of aeromonosis with saprolegniosis. The research was conducted at the Lviv Research Station of the Institute of Fisheries of NAAS. There were three groups of fish, 5 in each. The control group consisted of clinically healthy fish, the first experimental group (D1) – carps affected by Aeromonas hydrophila infection, the second experimental group (D2), which consisted of carps affected by associative Aeromonas hydrophila infection with Saprolegnia Sp. The results of studies have shown that carp with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. leads to the activation of lipid peroxidation processes, especially the primary product of LPO. Thus, in the hepatopancreas of fish there was an increase of 1.4 (p <0.05) in the reasonable content of diene conjugates and there was a tendency to increase the content of TBA-active products. At the same time, the inhibitory effect of pathogenic factors of this disease on the activity of the enzymatic link of the antioxidant defense system was stated. In particular, carp patients with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. had higher catalase and superoxide dismutase activity, respectively, 1.3 (p <0.05) and 2.3 (p <0.01) times. The results of experimental studies made it possible to expand and deepen the mechanisms of the pathogenesis of the co-infection of Aeromonas hydrophila infection with Saprolegnia Sp. and to use the data to develop effective treatments.

scholarly journals Peroxidative degradation of proteins and lipids in undifferentiated connective tissue dysplasia in children, Ukraine

Narra J ◽  
2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Tetyana V. Pochynok ◽  
Maryna M. Vasiukova ◽  
Iryna S. Kudlatska-Tyshko
Keyword(s):  
Lipid Peroxidation ◽  
Connective Tissue ◽  
Blood Plasma ◽  
Antioxidant Defense ◽  
Venous Blood ◽  
Colorimetric Method ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Protein Peroxidation ◽  
Connective Tissue Dysplasia

The aim of the study was to assess the state of lipid and protein peroxidation in children with undifferentiated connective tissue dysplasia (UCTD). In this study, 63 children (33 children with UCTD and 30 children without UCTD were recruited and the indicators of lipid and protein peroxidation were measured. The enzymatic, colorimetric method was used to measure the level of total cholesterol (TC). The phospholipids were analyzed by liquid chromatography/mass spectrometry using electrospray ionization. Lipid peroxidation was studied by assessing the change of index and the end product of lipid peroxidation, malondialdehyde (MDA), using spectrophotometric method. Protein peroxidation - by the content of carbonylated protein based on 2,4 dinitrophenyl-hydrazones derivatization. The activity of the antioxidant defense system enzymes was assessed by measuring catalase (CAT) and superoxide dismutase (SOD). Our study revealed a significant increase of PPO products in the venous blood plasma and LPO products in erythrocytes in children with UCTD. Furthermore, an imbalance of the antioxidant defense system was observed in both blood plasma and erythrocytes membrane.

Manganese and Zinc Overdose - Risk of Oxidative Stress Appearance

2008 ◽  
Vol 59 (9) ◽  
Author(s):  
Mirela Ahmadi ◽  
Mihaela Pup ◽  
Lucia Olariu ◽  
Horia Vermesan ◽  
Radu Prejbeanu
Keyword(s):  
Oxidative Stress ◽  
Trace Metals ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Hepatic Iron ◽  
Defense System ◽  
Amplification Effect ◽  
Enzymes Activity ◽  
Manganese Level

The study presents the effect of Zn and Mn overdoses in relation with other trace metals in rat�s liver. All studied metals are involved in some enzymes activity, which are implicated in antioxidant defense system of the body. But, in some conditions � our case in overdoses � an imbalance can appear, and antioxidant character of a metal can become prooxidant. In this case, antagonistic or synergic behaviour in relation with other trace metals is obvious. The observed modifications are the increasing of zinc and manganese level in liver after solutions administration, and the amplification effect over the hepatic iron, with the possibility of oxidative stress appearance.

scholarly journals NONINVASIVE METHODS FOR STUDYING THE INFLUENCE OF NONFERROUS METALLURGY EMISSIONS ON HUMAN HEALTH

2019 ◽  
Vol 96 (12) ◽  
pp. 1192-1195 ◽  
Author(s):  
V. N. Rakitskii ◽  
T. V. Yudina ◽  
Marina V. Egorova ◽  
N. S. Kutakova
Keyword(s):  
Heavy Metals ◽  
Trace Elements ◽  
Health Risk ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Oxidative Status ◽  
Nonferrous Metallurgy ◽  
The Body ◽  
Defense System ◽  
Methodological Approaches

There are presented methodological approaches to assess the risk of negative factors associated with characteristic areas of metallurgical enterprises in pollution of the environment with heavy metals to human health. The use of noninvasive biotesting, based on established correlations of indices of trace elements and oxidative status in the body is suggested. As a non-invasive biological material, alveolar moisture condensate from the person (expirate) is used. The basis for the health risk assessment is information on the balance of two components of the oxidative process responsible for the adaptive function of the body - free radical oxidation and antioxidant defense system. An imbalance of these processes contributes to the development of oxidative stress, the complication of the course of many diseases. The deviation in quantitative indices of a number of elements, primarily of metals of variable valency, which participate in reactions of lipid peroxidation, showing pro - or antioxidant properties can also serve as the indicator of the effects of complex heavy metals. The identified relationship of trace elements and oxidative status allows providing the approach to the assessment of processes of peroxidation in the assessment of microelement exchange indirectly. To study the intensity of peroxidation and capacity of the antioxidant defense system in expirate a number of methodological solutions was proposed - methods of chemiluminescence and spectrophotometry, as well as biotest, allowing detect deviations in the oxidative status and based on the level of bioluminescence of lyophilized bacteria by oxidative potential of investigated expirate of alveolar moisture. The complex of methods for assessment of the degree of the health risk associated with pollution by heavy metals can be used for screening observations in selected contingents with the purpose of formation of risk groups such as individually for the choice the optimal ways of correction of disorders and the assessment of the efficiency of preventive or corrective measures. Examples of methodological approaches in one of the regions of accommodation of the enterprises of nonferrous metallurgy, and in the estimation of risk development of pathological conditions and the efficiency of the treatment for a particular worker of the metallurgical plant, as the patient in the clinic of occupational diseases.

scholarly journals System of antioxidant protection of the body of piglets under the action of feed additive “Butaselmevit-plus”

2021 ◽  
Vol 4 (2) ◽  
pp. 38-43
Author(s):  
T. V. Martyshuk ◽  
B. V. Gutyj ◽  
V. I. Khalak
Keyword(s):  
Body Weight ◽  
Antioxidant Defense ◽  
Vena Cava ◽  
Antioxidant Defense System ◽  
The Body ◽  
Feed Additive ◽  
Before And After ◽  
Experimental Group ◽  
Technological Stress ◽  
Protective Systems

The aim of the study was to investigate the effect of the feed additive “Butaselmevit-plus” on the state of the antioxidant defense system of piglets during weaning. The experiments were conducted on the basis of the LTD “KOSHET” Mukachevo district of Zakarpattia region. Two groups of piglets were formed – control and experimental, in the amount of 10 individuals in each group, selected on the principle of analogs – age, breed and body weight. Animals were fed according to the norms for this age of pigs. On the 28th day of life, the piglets were weaned from the sow and regrouped from different nests for further maintenance during fattening and rearing with a change in the structure of the diet, which served as technological stress for the animal's body. Piglets of the experimental group, from 21 to 40 days of age, were additionally fed the feed additive “Butaselmevit-plus” at a dose of 100 mg/kg body weight per day. The material for the study was blood, which was taken in the morning before feeding the animals by puncture of the cranial vena cava for 20 days of life (the period before weaning), for 25 days of life (the period before weaning), for 30 days of life (2 days after weaning), for 35 days of life (7 days after weaning), 40 days of life (12 days after weaning). It is proved that feeding piglets feed additive “Butaselmevit-plus” enhanced the antioxidant status of piglets after weaning. It was found that under conditions of technological stress, the use of feed additive “Butaselmevit-plus” in the amount of 100 mg/kg body weight per day improves the antioxidant defense of the body, as evidenced by an increase in blood 35-day-old piglets superoxide dismutase activity by 26.7 % (P < 0.001), catalase – by 39.5 % (P < 0.001), glutathione peroxidase – by 51.7 % (P < 0.05), glutathione reductase – by 52.9 % (P < 0.05), the content of reduced glutathione – at 58.3 % (P < 0.001). These results confirm the effectiveness of the use of milk thistle, methionine, selenium, and vitamins A, E, and D3 in the feed additive “Butaselmevit-plus” to piglets in the period before and after weaning to activate the protective systems of their body.

scholarly journals The activity of the enzyme link of the antioxidant defense system of cows organism in experimental fasciolosis

2021 ◽  
Vol 23 (101) ◽  
pp. 3-7
Author(s):  
D. V. Frejuk ◽  
V. V. Stybel
Keyword(s):  
Redox Reactions ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
European Convention ◽  
Control Group ◽  
Defense System ◽  
Free Radical Reactions ◽  
Reversible Redox ◽  
Other Substances ◽  
Experimental Group

The antioxidant defense system of the organism controls and inhibits all stages of free radical reactions, starting from their initiation and ending to the formation of hydroperoxides and TBA-active products. The main mechanism of control of these reactions is associated with the chain of reversible redox reactions of metal ions, glutathione, ascorbate, tocopherol and other substances, the value of which is especially important for the preservation of long-standing macromolecules of nucleic acids and proteins, some components of membranes. The purpose of the research was to investigate the activity of the enzyme link of the antioxidant defense system of cows organism in experimental fasciolosis. Twelve cows of 4–5 years of age, Black-Spotted breed, were selected for the experiments, from which 2 groups were formed, six animals in each. Animals in control group (C) were clinically healthy. Animals of experimental group (E) were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed – selection and keeping of analogue animals in groups. The cows' ration was balanced in terms of nutrients and minerals. All animal manipulations were carried out in accordance with the European Convention for the Protection of Vertebrate Animals, which are used for experimental and scientific purposes. According to the results of our research, in cows affected by fasciolosis invasion, catalase activity in their blood decreased by 21.5 %, and superoxide dismutase activity by 30 %. In the research of the glutathione link of the antioxidant defense system, it was found that the activity of both glutathione peroxidase and glutathione reductase is also reduced in experimental fasciolosis. Thus, on the 25th day of the experiment, a decrease in these enzymes by 26.6 and 20.5 % is set. Thus, with the development of fasciolosis in ruminants, the balance in the complex “Antioxidant system – Lipid peroxidation” is disturbed, which in turn leads to complications of the disease. In the future it is planned to investigate the state of the non-enzymatic part of the antioxidant defense system of cows organism with experimental fasciolosis.

scholarly journals The effect of silicon compounds on hematological indicators and content of lipids in blood of rabbits

2018 ◽  
Vol 20 (92) ◽  
pp. 190-196 ◽  
Author(s):  
A. I. Ivanitskaya ◽  
Ya. V. Lesyk
Keyword(s):  
Body Weight ◽  
Blood Plasma ◽  
Fractional Composition ◽  
The Body ◽  
Control Group ◽  
Free Access ◽  
Animal Blood ◽  
Preparatory Period ◽  
Experimental Group ◽  
In Blood Plasma

The aim of the study was to find out the effect of casting of citrate nanosіlicon absorption and metasilicate sodium on the hematological parameters and lipid content and their fractional composition in blood plasma preparatory period 10 days prior to insemination and 20 days of lactation. The research was carried out on 60 rabbits of the Hyla hybrid, divided into three groups (control and two experimental), with 20 animals in each. The control animals were fed without limitation full-grain granulated feed with free access to water. Animals of the first experimental group (E-I) fed the diet of the control group and during the course of the day poured out the silicon citrate obtained using the nanotechnology method, 50 mkg Si/kg body weight with water. Samples of the second experimental group (E-II) feed the diet of the control group and set the sodium metasilicate (Na2SiO3H2O) 2.5 mg Si/kg body weight with water. The trial lasted 95 days, including the preparatory period of 10 days, the trial – 85 days. In the preparatory period of 10 days from the beginning of the study and in a trial of 20 days of lactation (65 days of supplements) in the rabbits, samples of blood from the marginal ear vein were taken. Hematologic studies were performed using the automatic hematologic analyzer “Orphee Mythic 18”, in the blood plasma, the content of total lipids and their fractional composition were determined. It was established that the number of erythrocytes and hemoglobin concentration in the blood of the rabbits of experimental group, which were given silicon citrate, were respectively higher by 15.2% (P < 0.05) and 12.0% (P < 0.01) by 20 days lactation versus control. This may indicate a more pronounced effect of the organic compound of silicon on the hematopoietic function of the body of the rabbits. According to white blood, the differences from control were found during the presentation of supplements with higher (P < 0.05) changes in 20 days of lactation in animal blood and experimental group. It was noted that the content of triacylglycerols in plasma of blood of groups I and II was lower by 31.2 and 32.8%, respectively (P < 0.05) compared to control. In blood plasma of animals in experimental groups an increase in the fractions of phosphatidylcholine, sphingomyelin and lysolecetin was observed compared to control. Such a redistribution was due to the reduction of the fractions of phosphatidic acid, phosphatidyl ethanolamine and phosphatidylinositol, and apparently, is associated with changes in the activity of the enzymes concerned. Hematological studies and determination of the fractional composition of lipids and phospholipids of blood plasma of the rabbits show positive changes that contribute to the metabolic accumulation of energy and plastic components of their organism.

scholarly journals Effect of Germinated Pigmented Rice “Superjami” on the Glucose Level, Antioxidant Defense System, and Bone Metabolism in Menopausal Rat Model

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2184
Author(s):  
Soo Im Chung ◽  
Tae-ho Ham ◽  
Mi Young Kang
Keyword(s):  
Body Weight ◽  
Bone Metabolism ◽  
Glucose Level ◽  
Rat Model ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Rice Cultivar ◽  
The Body ◽  
Defense System ◽  
Pigmented Rice

Women experience physical, mental, and social changes during menopause. It is important to maintain a healthy diet for effective menopause management. The effect of germinated Superjami, a deep violet colored rice cultivar, on the body weight, glucose level, antioxidant defense system, and bone metabolism in a menopausal rat model was investigated. The animals were randomly divided into three groups and fed with a normal diet (ND), a control diet supplemented with 20% (w/w) non-germinated Superjami flour (NGSF), or germinated Superjami flour (GSF) for eight weeks. The NGSF and GSF groups exhibited significantly lower body weight and fat, glucose and insulin contents, adipokine concentrations, and bone resorption biomarker levels, and higher antioxidant enzyme activities and 17-β-estradiol content than the ND group (p < 0.05). The GSF group showed greater glucose homeostasis, antioxidative, and bone metabolism-improving effects compared with the NGSF group. These findings demonstrate that germination could further improve the health-promoting properties of Superjami and that this germinated pigmented rice cultivar could be useful in the treatment and management of menopause-induced hyperglycemia, oxidative stress, and bone turnover imbalance.

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