The usefulness of re-attachability of anti-adhesive cross-linked gelatin film and the required physical and biological properties

2020 ◽  
Vol 31 (6) ◽  
pp. 351-360
Author(s):  
Tsunehito Horii ◽  
Hiroyuki Tsujimoto ◽  
Susumu Kageyama ◽  
Tetsuya Yoshida ◽  
Kenichi Kobayashi ◽  
...  
Keyword(s):  
Film Thickness ◽  
Tissue Injury ◽  
Biological Properties ◽  
Gelatin Film ◽  
Optimal Thickness ◽  
Physical Strength ◽  
Gelatin Films ◽  
Conventional Film ◽  
Buckling Test ◽  
Adhesion Effect

BACKGROUND: To overcome the unfavorable issues associated with conventional anti-adhesive HA/CMC film, we developed an anti-adhesive thermally cross-linked gelatin film. OBJECTIVE: We tried to clarify the re-attachability of the film and the required properties concerning the film thickness, stiffness and anti-adhesion effect. METHODS: To determine the optimal thickness, 5 kinds of the thickness of gelatin film and the conventional film were analyzed by the tensile test, shearing test, buckling test and tissue injury test. Finally, using the optimal film thickness, we tried to clarify the anti-adhesion effect of the reattached film. RESULTS: The tensile and shearing test showed gelatin films ≥30 μm thick had greater tensile strength and a smaller number of film fractures, than the conventional film. The buckling and tissue injury test showed gelatin films ≥60 μm thick had higher buckling strength and worse injury scores than the conventional film. The anti-adhesive effect of re-attached gelatin film using optimal thickness (30–40 μm) found the anti-adhesion score was significantly better than that of the control. CONCLUSIONS: Provided it has an optimal thickness, gelatin film can be reattached with enough physical strength not to tear, safety stiffness not to induce tissue injury, and a sufficient anti-adhesion effect.

scholarly journals Cytocompatibility and Suitability of Protein-Based Biomaterials as Potential Candidates for Corneal Tissue Engineering

2021 ◽  
Vol 22 (7) ◽  
pp. 3648
Author(s):  
Cristina Romo-Valera ◽  
Pedro Guerrero ◽  
Jon Arluzea ◽  
Jaime Etxebarria ◽  
Koro de la Caba ◽  
...  
Keyword(s):  
Soy Protein Isolate ◽  
Cell Types ◽  
Biological Properties ◽  
Protein Isolate ◽  
Sem Analysis ◽  
In Vitro Cytotoxicity ◽  
Gelatin Film ◽  
Corneal Tissue ◽  
Gelatin Films

The vision impairments suffered by millions of people worldwide and the shortage of corneal donors show the need of substitutes that mimic native tissue to promote cell growth and subsequent tissue regeneration. The current study focused on the in vitro assessment of protein-based biomaterials that could be a potential source for corneal scaffolds. Collagen, soy protein isolate (SPI), and gelatin films cross-linked with lactose or citric acid were prepared and physicochemical, transmittance, and degradation measurements were carried out. In vitro cytotoxicity, cell adhesion, and migration studies were performed with human corneal epithelial (HCE) cells and 3T3 fibroblasts for the films’ cytocompatibility assessment. Transmittance values met the cornea’s needs, and the degradation profile revealed a progressive biomaterials’ decomposition in enzymatic and hydrolytic assays. Cell viability at 72 h was above 70% when exposed to SPI and gelatin films. Live/dead assays and scanning electron microscopy (SEM) analysis demonstrated the adhesion of both cell types to the films, with a similar arrangement to that observed in controls. Besides, both cell lines were able to proliferate and migrate over the films. Without ruling out any material, the appropriate optical and biological properties shown by lactose-crosslinked gelatin film highlight its potential for corneal bioengineering.

scholarly journals Smart Starch-Gelatin Films Incorporated with Curcumin

2020 ◽  
Vol 36 (6) ◽  
pp. 1088-1095
Author(s):  
Le Thi Bich Nguyet ◽  
Vinh Tien Nguyen
Keyword(s):  
Acetic Acid ◽  
Food Packaging ◽  
Gelatin Film ◽  
Film Properties ◽  
Elongation At Break ◽  
Independent Variables ◽  
Gelatin Films ◽  
Film Forming ◽  
Central Composite Experimental Design ◽  
Central Composite

In this study, we developed a starch-gelatin film incorporated with synthesized curcumin to be used as a pH-sensitive smart material for food packaging. The film-forming mixture contained five components: starch, gelatin, glycerol, acetic acid and curcumin. The interactions of the components and their effects on the film properties were investigated by using response surface methodology with central composite experimental design. The results showed impacts of the contents of these components as independent variables on tensile strength, elongation at break, Young’s modulus and solubility of the films. The contents of starch, gelatin and glycerol significantly affect these properties, while acetic acid and curcumin do not (p<0.05). Also, it was shown that the incorporation of curcumin provided the film with the capacity to sense pH changes from neutral to basic (yellow at pH ≤ 8 and orange-red at pH ≥ 9).

Fish gelatin films laminated with emulsified gelatin film or poly(lactic) acid film: Properties and their use as bags for storage of fried salmon skin

2021 ◽  
Vol 111 ◽  
pp. 106199 ◽  
Author(s):  
Krisana Nilsuwan ◽  
Pedro Guerrero ◽  
Koro de la Caba ◽  
Soottawat Benjakul ◽  
Thummanoon Prodpran
Keyword(s):  
Lactic Acid ◽  
Gelatin Film ◽  
Poly Lactic Acid ◽  
Fish Gelatin ◽  
Film Properties ◽  
Gelatin Films

scholarly journals Physical and Biological Properties of a High-Plasticity Tricalcium Silicate Cement

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Arthur Dias Galarça ◽  
Wellington Luiz de Oliveira Da Rosa ◽  
Tiago Machado Da Silva ◽  
Giana da Silveira Lima ◽  
Neftalí Lenin Villarreal Carreño ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Film Thickness ◽  
Cell Viability ◽  
Water Solubility ◽  
Setting Time ◽  
Mineral Trioxide Aggregate ◽  
Biological Properties ◽  
High Plasticity ◽  
Silicate Matrix ◽  
Time Flow

Introduction. Mineral Trioxide Aggregate (MTA) is a tricalcium-based silicate, dicalcium silicate matrix. Despite its good biologic properties, some clinicians still claim to have difficulties in handling MTA after its preparation due to its sandy consistency. The aim of the present study was to evaluate the physicochemical properties and cytotoxicity of MTA Repair HP (Angelus, Londrina, PR, Brazil) compared with MTA Angelus (Angelus, Londrina, PR, Brazil). Materials and Method. The properties assessed were particle size, setting time, flow, film thickness, radiopacity, water solubility, compressive strength, and cytotoxicity. Statistical analysis was performed considering p < 0.05 as statistically significant. Results. For radiopacity, water absorption and solubility MTA Repair HP were statistically similar to MTA Angelus. The MTA Angelus had statistically different film thickness values, higher than MTA Repair HP (p < 0.05). Besides, MTA Angelus showed a lower and statistically different compressive strength after 28 days than MTA Repair HP (p<0.05). Additionally, MTA Repair HP set more slowly (p < 0.05). Relative to cell viability, MTA Repair HP was statistically similar to MTA Angelus after 24 and 48 h in cell viability. Conclusions. The MTA Repair HP presented similar cell viability, lower film thickness, higher flow, setting time, and compressive strength values after 28 days than MTA Angelus. In general, the MTA Repair HP presented physicochemical and biological properties similar to the MTA Angelus.

Biological properties and regulation of IL-10 related cytokines and their contribution to autoimmune disease and tissue injury

2012 ◽  
Vol 143 (2) ◽  
pp. 116-127 ◽  
Author(s):  
S.R. Hofmann ◽  
A. Rösen-Wolff ◽  
G.C. Tsokos ◽  
C.M. Hedrich
Keyword(s):  
Autoimmune Disease ◽  
Tissue Injury ◽  
Biological Properties

scholarly journals Antioxidant, Antibacterial, Water Binding Capacity and Mechanical Behavior of Gelatin-Ferula Oil Film as a Wound Dressing Material

2015 ◽  
Vol 4 (2) ◽  
pp. 103-14
Author(s):  
Gholamreza Kavoosi ◽  
Amin Shakiba ◽  
Mahmood Ghorbani ◽  
Seyed Mohammad Mahdi Dadfar ◽  
Amin Mohammadi Purfard
Keyword(s):  
Water Vapor ◽  
Wound Dressing ◽  
Water Solubility ◽  
Water Vapor Permeability ◽  
Antibacterial Activities ◽  
Gelatin Film ◽  
Vapor Permeability ◽  
Elongation At Break ◽  
Gelatin Films ◽  
Water Swelling

Background: Development of biodegradable and biocompatible films based on protein polymer with strong antioxidant and antibacterial activities has gradually obtained extensive concern in the world. In this study, the improvement of gelatin film properties incorporated with Ferula assa-foetida essential oil (FAO) as a potential antioxidant/antibacterial wound dressing film was investigated. Materials and Methods: Gelatin films were prepared from gelatin solutions (10% w/v) containing different concentration of FAO. The effect of FAO addition on water solubility, water swelling, water vapor permeability, mechanical behavior, light barrier properties as well as antioxidant and antibacterial activities of the films were examined. Results: Water solubility, water swelling and water vapor permeability for pure gelatin films were 29 ± 1.6%, 396 ± 8%, 0.23 ± 0.018 g.mm/m2.h, respectively. Incorporation of FAO into gelatin films caused a significant decrease in swelling and increase in solubility and water vapor permeability. Tensile strength, elastic modulus and elongation at break for pure gelatin films were 4.2 ± 0.4 MPa, 5.8 ± 4.2 MPa, 128 ± 8 %, respectively. Incorporation of FAO into gelatin films caused a significant decrease in tensile strength and elastic modulus and increase in elongation at break of the films. Gelatin film showed UV-visible light absorbance ranging from 280 to 480 nm with maximum absorbance at 420 nm. Gelatin/FAO films also exhibited excellent antioxidant ad antimicrobial activities. Conclusions: Our results suggested that gelatin/FAO films could be used as active films due to their excellent antioxidant and antimicrobial features for different biomedical applications including wound-dressing materials.[GMJ.2015;4(2):103-14]

Influence of anise (Pimpinella anisum L.) essential oil on the microbial, chemical, and sensory properties of chicken fillets wrapped with gelatin film

2020 ◽  
pp. 108201322093522
Author(s):  
Bahram Fathi-Achachlouei ◽  
Nima Babolanimogadam ◽  
Younes Zahedi
Keyword(s):  
Essential Oil ◽  
Shelf Life ◽  
Thiobarbituric Acid ◽  
Gelatin Film ◽  
Life Extension ◽  
Thiobarbituric Acid Reactive Substance ◽  
Chemical Parameters ◽  
Gelatin Films ◽  
Yeasts And Molds ◽  
Sensory Tests

The present study aimed to develop a novel active edible film based on gelatin incorporated with 0, 0.3, 0.6, and 0.9% w/w anise essential oil as a natural preservative and investigate the shelf life extension potential of chicken fillets during 12 days of refrigerated storage. The chicken fillets were wrapped with the essence-free and anise essential oil-loaded gelatin films, and microbial counts, chemical and sensory tests were surveyed during chilled storage. Results showed that aerobic mesophilic bacteria and Pseudomonas spp counts significantly decreased at all levels of anise essential oil during the first week of storage, while psychrotrophs, yeasts, and molds numbers began to reduce at concentrations of 0.6 and 0.9% from day 6. The using of anise essential oil caused a significant decrease of chemical parameters of chicken fillets, and the values of pH, peroxide, thiobarbituric acid reactive substance, and total volatile basic-nitrogen reached from 7.42, 5.7 meq/kg, 2.21 mg malondialdehyde/kg, and 24.94 mg N/100 g for the essence-free wrapped samples to 4.8, 6.35 meq/kg, 1.73 mg malondialdehyde/kg, and 18.78 mg N/100 g for the ones wrapped with 0.9% anise essential oil-loaded gelatin films at the end of storage day. In conclusion, application of gelatin films loaded with 0.6 and 0.9% anise essential oil can be advised for wrapping chicken fillets to prolong the shelf life for at least one week.

scholarly journals Phase Conjugation Using Four-Wave Mixing in Fast Green FCF Dye-Doped Gelatin Film

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
M. H. Majles Ara ◽  
S. Mehrabani ◽  
R. Malekfar
Keyword(s):  
Phase Conjugation ◽  
Four Wave Mixing ◽  
Gelatin Film ◽  
Total Power ◽  
Wave Mixing ◽  
Fast Green ◽  
Phase Conjugate ◽  
Gelatin Films ◽  
Fast Green Fcf ◽  
Acid Blue

We present optical phase conjugation based on the degenerate four-wave mixing (DFWM) arrangement in gelatin films doped with acid blue 3 dye (FAST GREEN FCF) using He-Ne laser of total power 50 mW. Various parameters which influence the phase-conjugate (PC) signal during the DFWM process were studied (angle of between the forward pump and the probe beam, transition of gelatin film, reflectivity as a function of forward and backward beam). A maximum phase-conjugate beam reflectivity of about 0.02% has been observed in these dye-doped gelatin films. PACS: 42.65.-k, 42.65.Hw.

scholarly journals Anisotropy Photoinduction during the Mass Associations of Dye Molecules in Gelatin Films

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Tariel Ebralidze ◽  
Nadia Ebralidze ◽  
Giorgi Mumladze
Keyword(s):  
Gelatin Film ◽  
Video Microscopy ◽  
Dye Molecules ◽  
Acridine Yellow ◽  
Gelatin Films ◽  
Saturated Solutions

By using the video microscopy, the picture of the formation of anisotropy photoinduction in the form of grains in time is shown, when anisotropy in the film is induced in individual micrograins and the concentration and sizes of the grains are modulated depending on the light exposition. This phenomenon was observed in the gelatin film dyed with the mixture prepared with the saturated solutions of Chrysophenine and Acridine Yellow mixed with 1 : 1 proportion.

Sign in / Sign up

Export Citation Format

Share Document