Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract

Recently, there has been a high demand from the population for dietary supplements that have antioxidant activity. First of all, these are dietary supplements with green tea leaf extract, because green tea contains catechins in a fairly high amount. Nowadays, according to the current legislation of Ukraine the qualitative composition and quantitative content of biologically active substances in dietary supplements are not established, which to a certain extent affects the quality and safety of these products. The aim of the study was to determine the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Materials and methods. Three dietary supplements from different manufacturers “Green Tea Extract”, “Green Tea Extract”, “Green Tea” were chosen for the study. Qualitative analysis was performed by thin layer chromatography, for quantification spectrophotometry in the UV-region was used. Results. The presence of catechins was found in the studied dietary supplements, caffeine was found only in trace amounts. The total content of catechins is 146.80 ± 1.36 mg, 79.00 ± 0.88 mg, 28.00 ± 0.75 mg, and in terms of green tea extract, which is stated in dietary supplements for dietary supplements “Green Tea Extract”, “Green Tea Extract”, “Green Tea”, respectively. Conclusions. Qualitative and quantitative analysis of some groups of BAS of three dietary supplements with green tea leaf extract was performed. It was found that dietary supplements “Green Tea Extract” and “Green Tea” meet the requirements of the US Pharmacopoeia 38 in terms of catechins. Based on the study, it can be concluded that the problem of compliance with dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

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Studi In-Vivo Ekstrak Daun Teh Hijau (Camellia Sinensis) sebagai Alternatif anti Bakteri Eschericia Coli pada Ayam Broiler

Jurnal Sain Veteriner ◽

10.22146/jsv.44953 ◽

2019 ◽

Vol 37 (2) ◽

pp. 172

Author(s):

Bambang Sutrisno ◽

R. Wasito ◽

Kurniasih Kurniasih ◽

Sitarina Widyarini ◽

Yuli Purwandari Kristianingrum ◽

...

Keyword(s):

Green Tea ◽

Broiler Chickens ◽

Leaf Extract ◽

Histopathological Examination ◽

Green Tea Extract ◽

E Coli ◽

Group B ◽

Tea Extract ◽

Tea Leaf

The prevalence of colibasillosis in chicken farms in Indonesia is very high, treatment using antibiotics is experiencing resistance, so it is necessary to look for alternatives to antibacterial. The study was aimed to determine the antibacterial effect of green tea leaf extract on broiler chickens infected with Eschericia coli by looking at the score of macroscopic lesions strengthened by histopathological examination, heterophile examination, plasma protein and fibrinogen. The research used 20 day old broilers (DOC) which were randomly divided into 4 groups, group A group B, group C and group D, each consisting of 5 DOC broilers. While maintaining ND and Gumboro vaccines on schedule like maintenance in general. At the age of 21 days all broilers in each group began to be treated as controls (Group A) without infecting E. coli and were not given 0,1g/ml water extract of green tea leaves (Camillia sinensis). Group B, intratracheal-infected broilers with local strains of E.coli were 108 cells / ml according to 0,5 Mc Farland standard, and were not given green tea leaf extract. Group C, broilers infected by intratracheal with local strains of E. coli 108 cells / ml by 0,5 Mc Farland standard, and given to drink green tea leaf extract (Camillia sinensis) 0,1 g/ml and group D, broilers were given drinking green tea leaf extract (Camillia sinensis) 0,1g/ml. During the treatment all of chickens were given food and drink ad libitum. Fourteen days after infection of E.coli, 5 chickens in each group were collected to collect blood for heterophyll, total plasma protein (TPP) and fibrinogen. And then were euthanasied with Mg SO4 saturated solution intravenously injection and necropsied for gross and histpathological examination. Analysis of blood tests results were used one way of anova (SPSS version 22 program), whereas for gross and histopathological examination with descriptive analysis. The results showed that the gross examination and histopathological organs of brolier infected with E. coli without being given a green tea extract experienced airsacculitis, pericarditis, perihepatitis and peritonitis, whereas broilers infected with E. coli and given green tea extract does not indicate the presence of inflammation. Examination of heterophile counts and blood fibrinogen levels had shown a difference (P <0.05), in broilers infected with E. coli and given green tea extracts had lower amounts of hetrophils and fibrinogen levels. While blood TPP levels were not significantly different (P> 0.05). The conclusion can be drawn, that the study of in vivo green tea extract (Camelia sinensis) 0,1g/ml has the potential to inhibit the infection of Eschericia coli bacteria in broiler chickens.

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Synthesis and Characterisation of Thin Films using Tanners Cassia, Nerium, Basil Leaf Extract Doped with Green Tea Extract Deposited by Single Dip Coating Method

Journal of Environmental Nanotechnology ◽

10.13074/jent.2015.09.153200 ◽

2015 ◽

Vol 4 (3) ◽

pp. 37-41

Keyword(s):

Thin Films ◽

Green Tea ◽

Leaf Extract ◽

Dip Coating ◽

Green Tea Extract ◽

Coating Method ◽

Dip Coating Method ◽

Tea Extract

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The study of the qualitative composition and the quantitative content of phenolic compounds in dietary supplements with lingonberry

Žurnal organìčnoï ta farmacevtičnoï hìmìï ◽

10.24959/ophcj.21.243782 ◽

2021 ◽

Vol 19 (4(76)) ◽

pp. 40-46

Author(s):

Oleksandr Yu. Maslov ◽

Mykola A. Komisarenko ◽

Serhii V. Kolisnyk ◽

Olga V. Antonenko ◽

Olena V. Kolisnyk ◽

...

Keyword(s):

Phenolic Compounds ◽

Dietary Supplements ◽

Total Content ◽

Hydroxycinnamic Acids ◽

Biologically Active ◽

Qualitative Composition ◽

Relevant Today ◽

Quantitative Content ◽

Layer Chromatography

Aim. Today, there are a lot of dietary supplements with lingonberry at the pharmaceutical market of Ukraine; therefore, the analysis and quality control of these products are relevant. In this connection, the aim of the research was to study the qualitative composition and determine the quantitative content of phenolic compounds in dietary supplements with lingonberry.Materials and methods. Such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM) were chosen for the study. The qualitative analysis was performed by thin layer chromatography (TLC), spectrophotometry was used for the quantitative determination.Results and discussion. Hydroquinone derivatives, flavonoids and hydroxycinnamic acids were found in the dietary supplements analyzed. The total content of phenolic compounds was 8.70, 0.26, 0.30 %, flavonoids – 6.37, 0.15, 0.12 %, hydroxycinnamic acids – 0.94, 0.06, 0.13 %, and hydroquinone derivatives – 1.01, 0.04, 0.03 % in such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM), respectively. Conclusions. The qualitative and quantitative analysis of the dietary supplements with lingonberry analyzed has been performed. “Extract of lingonberry” (MEDAGROPROM) dietary supplement meets the requirements of the State of Pharmacopoeia of Ukraine 2.0, whereas “Lingonberry” (Danikafarm) and “Lingonberry nano” (LSS SYSTEM) do not. Based on the results of the study it can be concluded that the problem of compliance of dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

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Study and evaluation antioxidant activity of dietary supplements with green tea extract

Current issues in pharmacy and medicine science and practice ◽

10.14739/2409-2932.2021.2.233306 ◽

2021 ◽

Vol 14 (2) ◽

pp. 215-219

Author(s):

O. Yu. Maslov ◽

S. V. Kolisnyk ◽

M. A. Komisarenko ◽

A. A. Altukhov ◽

K. V. Dynnyk ◽

...

Keyword(s):

Antioxidant Activity ◽

Dietary Supplements ◽

Green Tea ◽

Platinum Electrode ◽

Green Tea Extract ◽

Natural Sources ◽

Low Level ◽

A Value ◽

Potentiometric Measurements ◽

Tea Extract

The aim of the work is to determine and evaluate the antioxidant activity of dietary supplements with green tea extract, as well as to introduce conditional terms of the level of AOA. Materials and methods. The object of the study was dietary supplements of different manufactures: “Green Tea Extract” of Natural Sources, USA (DS1), “Extract of green tea” of Elit-Pharm, Ukraine (DS2) and “Green tea” of Pharmakom, Ukraine (DS3). Potentiometric measurements were conducted by pH meter Hanna 2550 (Germany) with a combined platinum electrode EZDO 5010. Epigallocatechin-3-O-gallate ˃98.0 % (Sigma Aldrich), K3[Fe(CN)6], K4[Fe(CN)6], NaHPO4, KH2PO4 were analytical grade. Results. It was established that a value of the antioxidant activity of DS1 was 36.51 mmol/tab, DS2 – 29.78 mmol/tab, and DS3 – 16.67 mmol/tab. DS1 had the highest value of the antioxidant activity, which correlated with the content of catechins (r2 = 0.9314). According to the proposed conditional terms of antioxidant activity dietary supplements, DS1 and DS2 possessed a low level of antioxidant activity, whereas DS3 corresponded to very low level of antioxidant activity. Conclusions. It was found that the studied dietary supplements with green tea extract have antioxidant activity, which correlates with the content of catechins.

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The influence of the granulation process on the qualitative composition and quantitative content of biologically active substances in oak bark

10.18411/spc-15-02-2019-04 ◽

2019 ◽

Author(s):

O. B. Trifonova ◽

O. V. Evdokimova

Keyword(s):

Biologically Active Substances ◽

Biologically Active ◽

Qualitative Composition ◽

Granulation Process ◽

Quantitative Content ◽

Active Substances

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Selected physicochemical and solubilization properties of pharmacopeal solutions of dry green tea leaf extract (Ext. Camellia sinensis L. folium aqu. siccum)

Herba Polonica ◽

10.2478/hepo-2018-0012 ◽

2018 ◽

Vol 64 (2) ◽

pp. 68-76

Author(s):

Zbigniew Marczyński ◽

Beata Skibska ◽

Sławomira Nowak ◽

Izabela Rychlińska ◽

Jerzy Jambor ◽

...

Keyword(s):

Green Tea ◽

High Performance ◽

Thermal Exposure ◽

Green Tea Extract ◽

Oral Dosage ◽

Solid Oral Dosage Form ◽

Preventive Activity ◽

Tea Extract ◽

Tea Leaf ◽

Risk Of Cancer

Summary Introduction: Green tea offers not only pleasant, delicate flavor, but also provides health benefits. The extract contains, among others, polyphenols responsible for antioxidant and anti-inflammatory properties. They reduce the risk of cancer and their presence exerts preventive activity against cardiovascular diseases. Objective: Analysis of selected physicochemical and solubilizing properties of pharmacopoeial-true solutions of dry green tea extract. Methods: The caffeine content was determined in the extract and in dry residue after solubilization by high performance liquid chromatography. The process of micellar solubilization of cholesterol granules and ketoprofen was carried out in model solutions of green tea extract. Results: The obtained results indicate that the prepared ‘ex tempore’ leaf green tea infusion subjected to short thermal exposure will be characterized by significant solubilization abilities. Conclusions: The outcomes of the research pointed to the possibility of developing a solid oral dosage form with titrated dry green tea extract of expected pharmacotherapeutic profile.

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Study on the composition of fatty and organic acids of the feverfew herb (Tanacetum Parthenium (L.) Schultz Bip.)

Farmatsevtychnyi zhurnal ◽

10.32352/0367-3057.5.20.07 ◽

2020 ◽

pp. 61-67

Author(s):

К. Р. Гордєй ◽

Т. М. Гонтова

Keyword(s):

Organic Acids ◽

Carboxylic Acids ◽

Mass Spectrometric ◽

Biologically Active Substances ◽

Biologically Active ◽

Mass Spectrometric Method ◽

Qualitative Composition ◽

Tanacetum Parthenium ◽

Quantitative Content ◽

Active Substances

In recent years, herbal medicines have become increasingly popular for the treatment of many diseases because they have a complex and multifactorial influence on the body. Fatty and organic acids are important groups of biologically active substances for the improvement of many pathological human’s conditions. Feverfew (Tanacetum parthenium (L.) Schultz Bip.) is a promising source of biologically active substances with many years of success in medicine and insufficiently studied chemical composition. The analysis of fatty and organic acids is one of the directions of our comprehensive study of the feverfew herb grown in Ukraine. The aim of the work was to conduct a study of the qualitative composition and quantitative content of fatty and organic acids in the feverfew herb grown in Ukraine. For the study, we used the feverfew herb, which was grown in the scientific fields of the Botanical Garden of the National University of Ukraine (2019). The studies were carried out by a gas chromatography-mass spectrometric method on an Agilent Technologies 6890N chromatograph with a 5973 mass spectrometric detector. 35 carboxylic acids were found and identified in the samples. Among them 21 acids are saturated, 5 are unsaturated and 9 are aromatic. The total content of carboxylic acids in feverfew herb was 26.54 mg/g. Among the identified fatty and organic acids in the feverfew herb the greatest amount of the following acids was observed: succinic (4.89 mg/g), levulinic (4.34 mg/g), citric (2.73 mg/g), palmitic (2.15 mg/g), linoleic (1.71 mg/g), oxalic (1.53 mg/g) and linolenic (1.07 mg/g) acids. Among the aromatic acids in the feverfew herb the dominant compounds were p-coumaric (2.4%), benzoic (1.89%) and ferulic (1.40%). For the first time, a chromatography-mass spectrometric determination of the qualitative composition and quantitative content of organic and fatty acids in feverfew herb collected in Ukraine was carried out. The data obtained indicate a varied composition and rich content of acids in domestic raw materials and will be used to create medicines based on the feverfew herb.

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