scholarly journals RESEARCH ON BIOCOMPATIBILITY OF CANCELLOUS - DERIVED ACELLULAR SCAFFOLD

2011 ◽  
Vol 14 (4) ◽  
pp. 74-84
Author(s):  
Quan Minh To ◽  
Buu Gia Tran ◽  
Oanh Thi Hoang Nguyen ◽  
Vu Nguyen Doan ◽  
Ngoc Kim Phan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Immune Responses ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cancellous Bone ◽  
Blood Cells ◽  
Umbilical Blood ◽  
Acellular Scaffold ◽  
Umbilical Blood Cells ◽  
Chloroform Methanol

Xenogeneic cancellous bone is a abundant resource on bone tissue engineering. But xenograft can arise a serious of immune responses. Thus, we decellularize xenogeneic cancellous bone for obtaining a great number of acellular and low antigenic scaffolds. Porcine cancellous bone (PCB) (4x4x3,5 cm3) were decellularized by acetone or chloroform/methanol (1:1). Biocompatibility of acellular scaffold was evaluated by seeding umbilical blood cells (UBCs) into scaffold and transplanting acellular scaffold to xeno-recipient. The result shows that PCB-derived scaffold treated in two decellularizing solutions have no cell. After 3 days, UBCs adhered on surface of scaffold and proliferated contiously after 12 days. After 8 days, scaffold makes a weak imflammatory response. The intial results show that PCB-derived acellular scaffold can apply in bone tissue engineering.

Preparation of Phosphorylated Chitosan/ Chitosan/ Hydroxyapatite Composites by Co-Precipitation Method

2009 ◽  
Vol 79-82 ◽  
pp. 401-404 ◽  
Author(s):  
Bin Li ◽  
Xin Bo Wang ◽  
Jin Huan Ma ◽  
Long Nan Huang
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cancellous Bone ◽  
Bending Strength ◽  
Weight Ratio ◽  
Strength Test ◽  
Precipitation Method ◽  
Co Precipitation

In this work, the PCS/CS/HA composites with different weight ratios were prepared through a co-precipitation method. The properties of these composites were characterized by means of the XRD, the IR, the SEM and the bending strength test. The value of bending strength of the PCS/CS/ HA composite with a weight ratio of 10/30/60 was measured about 34.93 MPa which is 1.6 times high of the cancellous bone. The composite is appropriate to be used as materials for bone tissue engineering.

Development of porous and antimicrobial CTS–PEG–HAP–ZnO nano-composites for bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (120) ◽  
pp. 99385-99393 ◽  
Author(s):  
Arundhati Bhowmick ◽  
Nilkamal Pramanik ◽  
Piyali Jana Manna ◽  
Tapas Mitra ◽  
Thirupathi Kumara Raja Selvaraj ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cancellous Bone ◽  
Nano Composites ◽  
Osteoblast Cell

We have developed porous, antimicrobial, biodegradable, and pH and blood compatible CTS–PEG–HAP–ZnO nanocomposites having good mechanical properties and osteoblast cell proliferation abilities to mimic cancellous bone in bone tissue engineering.

scholarly journals Synthesis and Characterization of Hierarchical Mesoporous-Macroporous TiO2-ZrO2 Nanocomposite Scaffolds for Cancellous Bone Tissue Engineering Applications

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Shima Mahtabian ◽  
Zahra Yahay ◽  
Seyed Mehdi Mirhadi ◽  
Fariborz Tavangarian
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cancellous Bone ◽  
Structural Integrity ◽  
Simultaneous Thermal Analysis ◽  
Engineering Applications ◽  
X Ray ◽  
Bet Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering has been introduced several decades ago as a substitute for traditional grafting techniques to treat bone defects using engineered materials. The main goal in bone tissue engineering is to introduce materials and structures which can mimic the function of bone to restore the damaged tissue and promote cell restoration and proliferation. Titania and zirconia are well-known bioceramics which have been widely used in tissue engineering applications due to their unsurpassed characteristics. In this study, hierarchical meso/macroporous titania-zirconia (TiO2-ZrO2) nanocomposite scaffolds have been synthesized and evaluated for bone tissue engineering applications. The scaffolds were produced using the evaporation-induced self-assembly (EISA) technique along with the foamy method. To characterize the samples, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), simultaneous thermal analysis (STA), and Brunauer-Emmett-Teller (BET) analysis were performed. The results showed that TiO2-ZrO2 scaffolds can be produced after sintering the samples at 550°C for 2 h. Among samples with different weight percentages of zirconia and titania, the sample containing 13 wt.% zirconia was considered as the optimum sample due to its structural integrity. This scaffold had pore size, pore wall size, and mesopores in the range of 185±66 μm, 15±4 μm, and 7-13 nm, respectively. The specific surface area obtained from the BET theory, total volume, and mean diameter of pores of this sample was 13.627 m2g1-, 0.03788 cm3g-1, and 11 nm, respectively. The results showed that the produced scaffolds can be considered as the promising candidates for cancellous bone regeneration.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

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