The Extract of Viola odorata Flowers Improves the Biochemical, Pancreas Histological, and Insulin Resistance Parameters in an Animal Model of Diabetes

Background: The antioxidant defense in the pancreas is low because they are exposed to toxic substances daily. This study aimed to evaluate the ameliorating effect of Viola odorata flowers Extract (VOE) on the pancreas histology and function in Streptozotocin (STZ)-induced diabetic rats. Methods: Forty male rats were divided into five groups, consisting of controls; STZ; and STZ plus various doses of VOE (100, 200 or 400 mg/kg). The amylase, lipase, insulin and total antioxidant capacity levels were measured in the sera. The homeostatic model assessment of insulin resistance was also measured. The histopathological alterations of the rats’ pancreases were examined microscopically. Results: The serum amylase and total antioxidant activities were reduced in diabetic rats (P=0.001). Varying doses of VOE reduced the serum amylase and glucose levels, and increased the total antioxidant activities compared to that of the diabetic rats (P<0.05). There were no significant differences in the serum lipase and insulin levels among the groups. Treatment with VOE at all doses significantly lowered the insulin resistance compared to that of the diabetic group (P=0.001). Significant reductions were observed in the areas of the pancreatic Langerhans islets and the number of beta cells in the STZ group (P=0.001). Conclusion: This study demonstrated that VOE ameliorated the adverse effects induced by STZ in the rat’s pancreas in the short-term. These effects are likely to be due to the reduced insulin resistance and amylase activity, and increased total antioxidant activity along with the histopathological alterations in the pancreas.

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The Effect of Tianmai Xiaoke Pian on Insulin Resistance through PI3-K/AKT Signal Pathway

Journal of Diabetes Research ◽

10.1155/2016/9261259 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Nana Wang ◽

Tiegang Li ◽

Ping Han

Keyword(s):

Insulin Resistance ◽

Fasting Blood Glucose ◽

Area Under The Curve ◽

Diabetic Rats ◽

Signal Pathway ◽

Chromium Picolinate ◽

Oral Glucose ◽

Model Assessment ◽

Insulin Sensitizer ◽

Type 2 Diabetic Mellitus

In the clinical setting, given the potential adverse effects of thiazolidinediones and biguanides, we often have difficulty in treatment that no other insulin sensitizers are available for use in type 2 diabetic mellitus (T2DM) patients. Tianmai Xiaoke Pian (TMXKP) is a traditional Chinese medicine tablet, which is comprised of chromium picolinate, Tianhuafen, Maidong, and Wuweizi. To understand its mechanism of action on insulin resistance, TMXKP (50 mg/kg orally) was tested in T2DM rats (induced by a high-fat diet and streptozotocin). Eight weeks later, fasting blood glucose (FBG) and oral glucose tolerance tests (OGTT) were performed. Area under the curve (AUC) and homeostatic model assessment of insulin resistance (HOMA-IR) were calculated, and PI3-K/AKT signal pathway-related genes and proteins were tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis in muscle, adipose, and liver tissues, respectively. TMXKP significantly reduced FBG, OGTT, AUC, and HOMA-IR in diabetic ratsP<0.05. Furthermore, we also observed that TMXKP could significantly decreaseIRS-1,IRS-2,PI3-K p85α, andAKT2gene expression and also IRS-1, IRS-2, PI3-K, AKT2, and p-AKT2 protein expression levelsP<0.05in diabetic rats. These findings confirm that TMXKP can alleviate insulin resistance in T2DM rats through the PI3K/AKT pathway. Thus TMXKP appears to be a promising insulin sensitizer.

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Evaluation of Antidiabetic and Antihyperlipidemic Effects of Peganum harmala Seeds in Diabetic Rats

Cholesterol ◽

10.1155/2016/7389864 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Gholamreza Komeili ◽

Mohammad Hashemi ◽

Mohsen Bameri-Niafar

Keyword(s):

Total Cholesterol ◽

Glycosylated Hemoglobin ◽

Diabetic Rats ◽

Male Rats ◽

Peganum Harmala ◽

Hydroalcoholic Extract ◽

End Of Treatment ◽

Total Antioxidant ◽

Treatment Of Diabetes ◽

Different Doses

The present study was carried out to investigate the antidiabetic and antihyperlipidemic properties of hydroalcoholic extract of Peganum harmala in streptozotocin-induced diabetic male rats. In an experimental study, 64 normal Wistar albino male rats (200–230 g) were randomly divided into 8 groups. Control and diabetic rats were treated with normal saline and three different doses (30, 60, and 120 mg/kg) of hydroalcoholic extract of Peganum harmala seeds for 4 weeks orally. At the end of treatment, blood samples were taken and glucose, triglycerides, total cholesterol, LDL-c, HDL-c, malondialdehyde (MDA), total antioxidant capacity (TCA), ALT, AST, GGT, bilirubin, and glycosylated hemoglobin (HbA1C) were determined. STZ-induced diabetic rats showed significant changes in the values of glucose, triglycerides, total cholesterol, LDL-c, MDA, TAC, ALT, AST, GGT, bilirubin, and HbA1C in comparison with normal rats. Administration of the extract to diabetic rats resulted in a remarkable decrease in glucose, lipid profiles, MDA, ALT, AST, GGT, bilirubin, and HbA1C levels and increase in TAC relative to diabetic group. The results of this study indicated that hydroalcoholic extract of Peganum harmala seeds possesses antidiabetic and hypolipidemic activities and could be useful in treatment of diabetes.

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The improvement of insulin resistance and the antioxidant capacity in type 2 diabetes mellitus rats with whiteleg shrimp shell powder (Litopenaeus vannamei)

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1684 ◽

2021 ◽

Vol 15 ◽

pp. 703-711

Author(s):

Risya Ahriyasna ◽

Tri Winarni Agustini ◽

Kis Djamiatun ◽

Def Primal

Keyword(s):

Insulin Resistance ◽

Antioxidant Capacity ◽

Model Assessment ◽

Treatment Groups ◽

Shrimp Shell ◽

Homeostatic Model Assessment ◽

Total Antioxidant ◽

Research Findings ◽

Shell Powder

As estimated having an increased incidence of about 50% until 2040, the diabetic condition could be augmented primarily from astaxanthin contained in carotenoids. This research examines and compares the influence of WSSP and AST complement on Homeostatic Model Assessment-Insulin Resistance (HOMA-IR) level and Total Antioxidant Capacity (TAC) induced high-fat diet streptozotocin (HFD-STZ) in T2DM rats. WSSP 0.89gr/200gr/d (X1) and 1.77gr/200gr/d (X2) groups; and AST supplement 0.09mg/200gr/d (X3) groups were compared with both of positive (K1) and negative (K2) groups. The treatments were administered orally for 21 days to 25 Wistar rats which each group consisted of 5 rats. HOMA-IR and TAC levels were measured by ELISA and ABTs method respectively. TAC levels significantly increased in treatment groups than K1 group (p = 0.008). The increase in the TAC level of the X2 group was greater than the X1 group (p = 0.017). HOMA IR levels were significantly lower in treatment groups than K1 group (p = 0.009). X2 group had a greater decrease in HOMA IR levels compared to X1 (p = 0.016). In consequence, the research findings show a utilitarian effect of WSSP in increasing TAC and decreasing the HOMA-IR index.

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Effects of Namya Kanom Jeen powder extract on hypoglycemic and anti-oxidant properties in Alloxan-induced diabetic rats

Bioactive Compounds in Health and Disease ◽

10.31989/bchd.v1i8.549 ◽

2018 ◽

Vol 1 (8) ◽

pp. 86

Author(s):

Preeya Dat-arun ◽

Rattana Leelawattana ◽

Pavinee Chinachoti

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Blood Glucose ◽

Water Extract ◽

Diabetic Control ◽

Diabetic Rats ◽

Water Soluble ◽

Model Assessment ◽

Homeostatic Model Assessment ◽

Anti Oxidant

Background: Diabetes mellitus (DM) is a major health care problem worldwide. Major intervention control of DM is by using medical treatments and dietetic therapy. Spices and herbs have been known to have anti-oxidant, anti-inflammation and anti-diabetic properties. Southern Thai foods known to contain phytochemicals in large amount, have been demonstrated to exhibit such properties and Namya Kanom Jeen (NKJ), a Southern Thai soup, shown to be most promising. Here, we studied the effect of NKJ water extracts on hypoglycemic and anti-oxidant properties in Alloxan-induced diabetic rats.Methods: This study aimed to assess the effect of NKJ water extract on blood glucose, insulin, malondialdehyde (MDA), homeostatic model assessment of insulin resistance (HOMA-IR) and high sensitive C-reactive protein (hs-CRP) levels in Alloxan-induced diabetic (DM) rats for 3 weeks.Results: In Alloxan-induced diabetic rats, the NKJ water soluble extracts at 200, 1,000 and 2,000 mg/kg body weight doses (n=7) significantly lowered blood glucose, insulin, MDA, HOMA-IR levels compared to diabetic control (Metformin, p < 0.05). Conclusion: In summary, feeding of NKJ aqueous extract effectively lowered baseline blood glucose, insulin, MDA and HOMA-IR in diabetic rats.Keywords: Diabetes mellitus; Anti-oxidant; Glycemic; Insulin resistance; HOMA-IR, Namya Kanom Jeen powder

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High Fiber and Beta Carotene from Sweet Potatoes and Pumpkin Improve Insulin Resistance by Inhibition of Sterol Regulatory Binding Protein 1c in Liver of Hypertriglyceridemic Rats

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.5354 ◽

2020 ◽

Vol 8 (A) ◽

pp. 898-903

Author(s):

Sunarti Sunarti ◽

Umar Santoso ◽

Abrory Agus Cahya Pramana ◽

Emy Huriyati ◽

Dianandha Septiana Rubi

Keyword(s):

Gene Expression ◽

Insulin Resistance ◽

Binding Protein ◽

Beta Carotene ◽

Male Rats ◽

Standard Diet ◽

Model Assessment ◽

High Fat ◽

High Fiber ◽

Improve Insulin Resistance

BACKGROUND: High sterol regulatory binding protein 1c (SREBP-1c) gene expression increases triglyceride synthesis, which induces insulin resistance. Short-chain fatty acids (SCFAs) from fiber fermentation and beta carotene may inhibit SREBP-1c gene expression. AIM: The aim of this study was to evaluate the high fiber and beta carotene diet on improving insulin resistance in hypertriglyceridemia rats. METHODS: A total of 25 Wistar male rats were divided into five groups: (1) Normal control (NC); (2) hypertriglyceridemia control (HC); (3) hypertriglyceridemia rats with treatment 1 (HT1); (4) hypertriglyceridemia rats with treatment 2 (HT2); and (5) hypertriglyceridemia rats with treatment 1 (HT3). The HT1, HT2, and HT3 received fiber 1.0 g; 2.0 g; and 3.1 g and beta carotene 725.7 μg; 1451.5 μg; and 2177.2 μg per day, respectively, for 6 weeks. The HC received high fat and fructose diet and the NC received a standard diet. The levels of triglyceride were analyzed using the colorimetric method before and after treatment. At the end of the study, the expression of SREBP-1c was identified by a quantitative polymerase chain reaction. RESULTS: High fat and fructose diet increased the levels of triglyceride (36.53 ± 1.27 vs. 119.79 ± 7.73), but high fiber and beta carotene diet can reduce triglyceride levels in HT1 (94.58 ± 4.53 vs. 77.70 ± 7.97); HT2 (115.58 ± 4.76 vs. 66.90 ± 3.11); and HT3 (103.87 ± 7.47 vs. 62.06 ± 4.45). The decreased triglyceride levels were related to low SREBP-1c gene expression, especially in the liver. Low SREBP-1c gene expression was correlated with homeostatic model assessment of insulin resistance index with r = 0.414; p < 0.05 in the liver and r = 0.158; p > 0.05 in white adipose tissues. CONCLUSION: High fiber and beta carotene diet can improve insulin resistance through inhibition of SREBP-1c gene expression.

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Anethum graveolens L‎. Alleviates Sperm ‎Damage by Limiting Oxidative Stress ‎and Insulin ‎Resistance ‎in ‎Diabetic Rats ‎

The Open Medicinal Chemistry Journal ◽

10.2174/1874104502014010035 ◽

2020 ◽

Vol 14 (1) ◽

pp. 35-44

Author(s):

Ebrahim Abbasi-Oshaghi ◽

Iraj Khodadadi ◽

Fatemeh Mirzaei ◽

Mehrdad Ahmadi ◽

Heidar Tayebinia ◽

...

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Morphological Changes ◽

Diabetic Rats ◽

Male Rats ◽

Diabetic Rat ◽

Anethum Graveolens ◽

Sod Activity ◽

Histological Changes ◽

Anti Oxidant

Background: It has been reported that diabetes is associated with sperm ‎damage and infertility. Objective: The purpose of this experiment was to survey the effect of Anethum graveolens L. (Dill) powder on sperm profiles, oxidative stress, insulin resistance, and histological changes in male diabetic rats. Methods: Male rats were randomly divided into 6 groups (n=7); group 1: normal rats, 2: normal rats + 100mg/kg Dill, 3: normal rats + 300mg/kg Dill, 4: diabetic rats, 5: diabetic rats + 100mg/kg Dill, and 6: diabetic rats + 300mg/kg Dill. After 2 months of treatments, the sperm profile, anti-oxidant activity, superoxide dismutase (SOD) activity, and malondialdehyde were measured. The histopathology of testis was evaluated. Hormonal changes and tumor necrosis factor-α (TNF-α) levels were measured by ELISA. Results: Total anti-oxidant and SOD activity in diabetic rats significantly decreased, while MDA concentration was significantly increased in the testis and pancreas of diabetic rats compared with control. However, the use of Dill significantly normalized these profiles. The treatment of diabetic rats with Dill changed the sperm parameters. The levels of testosterone, FSH, and LH in diabetic rats were significantly reduced, but the treatment with Dill did not alter the level of these hormones. Dill also significantly normalized testis morphological changes, insulin resistance, and inflammation. Conclusion: The use of Dill normalized oxidative stress, inflammation, and insulin resistance in diabetic rats that correlated with sperm profile and testis histological changes. The treatment of diabetic rat models with Dill did not show harmful effects on sperm profiles.

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Antihyperglycemic Activities of Extracts of the Mistletoes Plicosepalus acaciae and P. curviflorus in Comparison to their Solid Lipid Nanoparticle Suspension Formulations

Zeitschrift für Naturforschung C ◽

10.5560/znc.2014-0047 ◽

2014 ◽

Vol 69 (9-10) ◽

pp. 391-398 ◽

Cited By ~ 4

Author(s):

Hibah M. Aldawsari ◽

Abeer Hanafy ◽

Gihan S. Labib ◽

Jihan M. Badr

Keyword(s):

Insulin Resistance ◽

Blood Glucose ◽

Antioxidant Activities ◽

Diabetic Animal ◽

Diabetic Rats ◽

Solid Lipid Nanoparticle ◽

Nanoparticle Suspension ◽

Solid Lipid ◽

Lipid Nanoparticle

Abstract The antihyperglycemic activity of the extracts and preparations of solid lipid nanoparticle suspensions of two mistletoes growing in Saudi Arabia, Plicosepalus acaciae and P. curviflorus, as well as their possible antioxidant effect were investigated in a type 2 diabetic animal model. Type 2 diabetes was induced in adult maleWistar rats by a high-fat diet followed by injection of streptozotocin (STZ). The diabetic rats were treated in parallel with pioglitazone hydrochloride (PIO), non-toxic extracts of P. acaciae and P. curviflorus, as well as three different solid lipid nanoparticle (SLN) suspension formulations prepared from each of the two extracts. Blood glucose level, insulin resistance, oxidative stress parameters, and antioxidant markers were determined. The total extracts of P. acaciae and P. curviflorus as well as the SLN formulations exhibited a significant blood glucose-lowering effect associated with antioxidant effects in the diabetic rats. The SLN preparation with the highest lipid content gave the best result. Reduction of hyperglycemia and insulin resistance in the diabetic rats was, at least partly, due to the antioxidant activities of the extracts and their SLN formulations.

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Pengaruh Asam Alfa Lipoat Terhadap Kadar Trigliserida pada Tikus Diabetes Melitus Tipe 2

International Journal of Seocology ◽

10.26891/jik.v12i1.2018.13-18 ◽

2018 ◽

Vol 12 (1) ◽

pp. 13

Author(s):

Angel Chen ◽

Ismawati Ismawati ◽

Enikarmila Asni

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Triglyceride Level ◽

Diabetic Rats ◽

Male Rats ◽

Alpha Lipoic Acid ◽

Type 2 Diabetic ◽

Wistar Male Rats ◽

Diabetes Melitus

Accumulation of triglycerides associated with insulin resistance in type 2 diabetes mellitus (T2DM). Alpha lipoic acid (ALA) has hypotriglyceridemic effects by lowering triglyceride levels in blood and liver. This study aims to analyze effect of ALA on triglyceride levels in type 2 diabetic rats. Fifteen Wistar male rats (±8-12 weeks, 200-250 gram) divided into 3 groups (control, T2DM and T2DM–ALA given for 3 weeks). Diabetes was induced by giving streptozotocin (50 mg/kg.bw) and nicotinamide (110 mg/kg.bw) after 15 minutes intraperitoneally. Diagnosis of T2DM was determined if the rats have perifer blood glucose >250 mg/dL after 3 days. The results showed that triglyceride levels in ALA group (p=0,003) lower than T2DM group, T2DM group (p=0,031) had higher triglyceride level than control and no difference of triglyceride level between T2DM-ALA given group with control (p=0,696). This study concluded that ALA can affect triglyceride levels in T2DM thus prevents occurrence of hypertriglyceridemia.

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Evaluation of Tribulus terrestris Extracts Relative to Metformin on Oxidative Stress and Histopathology of the Liver for Diabetic Male Rats

Diabetology ◽

10.3390/diabetology3010004 ◽

2022 ◽

Vol 3 (1) ◽

pp. 46-55

Author(s):

Rasha A. Al-Eisa ◽

Hend M. Tag ◽

Mohamed Salah ElNaggar ◽

Heba M. A. Abdelrazek ◽

Nahla S. El-Shenawy

Keyword(s):

Oxidative Stress ◽

Antioxidant Activities ◽

Morphological Changes ◽

Rat Hepatocytes ◽

Diabetic Rats ◽

Male Rats ◽

Dependent Diabetes Mellitus ◽

Control Group ◽

Tribulus Terrestris ◽

Ros Scavenging

Insulin-dependent diabetes mellitus (IDDM) is a metabolic condition that induces blood glucose levels to rise due to insulin deficiency and the formation of reactive oxygen species (ROS). The purpose of this study is to assess how efficient the antioxidant extracts Tribulus terrestris (TT) and metformin (MET) are in reducing oxidative stress and histopathology produced by streptozotocin in rat hepatocytes. The 36 male rats weighing 170–190 g of this study were randomly sorted into 6 groups. The first group was considered a normal control group, and the second and third groups were normal and remedy with MET and TT extract, respectively. The fourth group was positive diabetic, and the fifth and sixth groups were diabetic rats that were treated with MET and TT extract, respectively. Lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST), and glutathione (GSH) were detected, and the histopathology of the liver was evaluated after 8 weeks of treatment. Compared to regulation, morphological changes in the liver were found in diabetic animals, with a rise in LPO and a change in GSH levels as well as CAT and GST activities. The oxidative stress and histological architecture of the hepatocytes caused by hyperglycemia were improved as a result of therapy in the rats with MET and TT extract. Because of its antioxidant activities, diabetic rats with TT extract are more effective than MET in normoglycemia and hepatocyte reconditioning. Beneficial intervention tends to benefit primarily from direct ROS scavenging and CAT, GST, and GSH regeneration.

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Comparative Study on the Influence of Some Medicinal Plants on Diabetes Induced by Streptozotocin in Male Rats

BioMed Research International ◽

10.1155/2019/3596287 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 4

Author(s):

Daklallah A. Almalki ◽

Sameera A. Alghamdi ◽

Atef M. Al-Attar

Keyword(s):

Oxidative Stress ◽

Saudi Arabia ◽

Medicinal Plants ◽

Diabetic Control ◽

Diabetic Rats ◽

Male Rats ◽

Therapeutic Effects ◽

Oxidative Stress Markers ◽

Histopathological Alterations ◽

Stress Markers

Medicinal plants have played an important role in the treatment of many diseases. Medicinal plants are believed to be well appropriate with the human body and to produce less side influences than the pharmaceuticals. Kingdom of Saudi Arabia has abundant and wide variety of medicinal plants whose therapeutic effects have not been adequately studied. The aim of this study was to investigate the hypoglycemic activities of the extracts of three plant species collected from Albaha region of Saudi Arabia including Olea oleaster (Oleaceae family) leaves (OLE), Juniperus procera (Cupressaceae family) leaves (JLE), and Opuntia ficus-indica (Cactaceae family) stems (OSE) on streptozotocin (STZ) diabetic male rats. The animals were distributed into eight groups. The first group was used as normal control. The second group was diabetic control. Diabetic rats of the third, fourth, and fifth groups were supplemented with OLE, JLE, and OSE, respectively. Normal rats of the sixth, seventh, and eighth groups were treated with OLE, JLE, and OSE, respectively. As expected, the mean of body weight was significantly decreased in rats of the second group. Significant increase in the value of serum glucose and decline of insulin value were observed in rats of the second group. Several alterations of lipid and protein profile and oxidative stress markers were noted in diabetic control rats. Severe histopathological alterations of pancreatic tissues were observed in untreated diabetic rats. The obtained results showed that OLE, JLE, and OSE attenuated the physiological and histopathological alterations. These new data indicate that the attenuation influences of OLE, JLE, and OSE attributed to their antioxidant properties confirmed by oxidative stress markers evaluation.

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