scholarly journals Histochemical method of diagnosis of mixed pasteurellosis and ascaridosis bird disease

2018 ◽  
Vol 20 (92) ◽  
pp. 142-144
Author(s):  
V. M. Plys ◽  
T. I. Fotina
Keyword(s):  
Blood Vessels ◽  
Pasteurella Multocida ◽  
Histological Section ◽  
Histochemical Method ◽  
Control Group ◽  
Ascaridia Galli ◽  
Smooth Muscle Tissue ◽  
Violet Color ◽  
Purple Color ◽  
Experimental Group

For the first time was developed an effective and specific histochemical method for the diagnosis of mixed pasteurellosis and ascaridosis diseases of birds, which can be used in conjunction with bacteriological, serological and helminthocoproscopic studies, and in cases where it is impossible to diagnose serologic methods. The proposed method of histochemical diagnostics can be used for the detection of pasterelo-ascaridium carriers during mixed pasteurellosis and ascaridosis disease of birds. In the birds of the experimental group (clinically diseased bird), the pasteurial antigen in the histological preparations of the organs tissues after the histochemical study looked like local and diffuse clusters of oval and deep form of intense purple color and local clusters of oblong-shaped larvae with the presence of a head and a tail of blue and eggs of light purple color On histological sections of tissues and organs of the control group (clinically healthy bird), such changes were absent. For investigation of the organs of the experimental group (clinically diseased poultry), we have established the following areas of accumulation of pasteurial antigen and larvae and eggs of ascarids: in the heart – observed postponement of the antigen Pasteurella multocida in the form of strips throughout the area of the histological cut along the length of the muscle fibers and the loss of their delineation and fragmentation; in the liver – observed the diffuse location of the Pasteurella multocida antigen throughout the histological section in the lumen of the blood vessels and around them, the Disse spaces, between the hepatocytes; histochemically detected reduction of AsAT – by 84.3% and bilirubin – by 74.5%; in the muscular stomach – there was a diffuse postponement of the pasteurial antigen throughout the histologic section, but the largest accumulation was observed around the fibers of smooth muscle tissue of dark purple; in the spleen – the presence of Pasteurella multocida antigen throughout the histological section around lymphoid clusters, blood vessel walls, and intensive deposition of the antigen were observed around the splenic artery in the form of a deep, intense violet color; in the duodenum – observed diffuse deposition of the Pasteurella multocida antigen across the entire histologic area around the blood vessels, lymphatic follicles, villi of the mucous membrane, between the enterocytes, the mucosal muscle folds, the intramural ganglion nodules in the form of the deep form; Intestinal wall, blood vessels, licorice glands, larvae of ascarid species of Ascaridia galli species were observed blue, around granular grains around the dead larvae; Histochemically, decreased activity of alkaline phosphatase – by 70% and mucin – by 82.4%; in the kidneys – locally observed the presence of a Pasteurella multocida pasteur antigen around the intercellular veins, a capsule of the nephron, a circular oval-shaped distal tubule, a dark violet color; Histochemically, creatinine decreased by 45.4%.

scholarly journals The Effect of Subconjunctival Bevacizumab on Angiogenesis in Rabbit Model

2020 ◽  
Vol 55 (4) ◽  
pp. 290
Author(s):  
Nurwasis Nurwasis ◽  
Diana Yuliawati ◽  
Evelyn Komaratih ◽  
Heriyawati Heriyawati
Keyword(s):  
Blood Vessels ◽  
Healing Process ◽  
Rabbit Model ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Histopathological Analysis ◽  
Subconjunctival Injection ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

The wound healing process is major cause of glaucoma surgery failure and enhances the incapability of controlling the IOP. This aim of study was to evaluate the angiogenesis effect of Bevacizumab injection on the amount and density of blood vessels in the rabbit model after trabeculectomy. It was a true experimental study using 16 eyes of 16 New Zealand White Rabbit (Oryctolagus cuniculus) that divided into two groups. The control group was treated with 0.05 ml of Balanced Saline Solution (BSS). The experimental group was treated with subconjunctival injection of 1.25 mg Bevacizumab in 0.05 BSS. All rabbit was sacrificed, and the eye was enucleated. Thus, the bleb area was dissected after 14 days. Histopathological analysis was performed to evaluate the amount and density of blood vessels. The mean amount of blood vessels in the control group was 22.63 ± 11.02, and the experimental group was 14,75 ± 4.92 (p=0.043). The mean of blood vessel density in the control group was 19.10 ± 1.69 %, and the experimental group was 16.53 ± 2.90 % (p=0.029)%. There was a statistically significant difference between the two groups (p<0.05). Subconjunctival bevacizumab in the rabbit model reduces the amount and density of blood vessels compared with subconjunctival BSS injection.

scholarly journals The Effect of Subconjunctival Bevacizumab on Angiogenesis in Rabbit Model

2021 ◽  
Vol 55 (4) ◽  
pp. 290
Author(s):  
Nurwasis Nurwasis ◽  
Diana Yuliawati ◽  
Evelyn Komaratih ◽  
Heriyawati Heriyawati
Keyword(s):  
Blood Vessels ◽  
Healing Process ◽  
Rabbit Model ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Histopathological Analysis ◽  
Subconjunctival Injection ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

The wound healing process is major cause of glaucoma surgery failure and enhances the incapability of controlling the IOP. This aim of study was to evaluate the angiogenesis effect of Bevacizumab injection on the amount and density of blood vessels in the rabbit model after trabeculectomy. It was a true experimental study using 16 eyes of 16 New Zealand White Rabbit (Oryctolagus cuniculus) that divided into two groups. The control group was treated with 0.05 ml of Balanced Saline Solution (BSS). The experimental group was treated with subconjunctival injection of 1.25 mg Bevacizumab in 0.05 BSS. All rabbit was sacrificed, and the eye was enucleated. Thus, the bleb area was dissected after 14 days. Histopathological analysis was performed to evaluate the amount and density of blood vessels. The mean amount of blood vessels in the control group was 22.63 ± 11.02, and the experimental group was 14,75 ± 4.92 (p=0.043). The mean of blood vessel density in the control group was 19.10 ± 1.69 %, and the experimental group was 16.53 ± 2.90 % (p=0.029)%. There was a statistically significant difference between the two groups (p<0.05). Subconjunctival bevacizumab in the rabbit model reduces the amount and density of blood vessels compared with subconjunctival BSS injection.

Effect of Antiplatelet Therapy Combining Aspirin with Tirofiban After Percutaneous Coronary Intervention on the Incidence of Re-occlusion of Blood Vessels and Platelet Aggregation Rate in Patients with Acute Myocardial Infarction

2021 ◽  
Vol 7 (5) ◽  
pp. 4049-4056
Author(s):  
Tongtong Wu ◽  
Fang Cheng
Keyword(s):  
Platelet Aggregation ◽  
Blood Vessels ◽  
Antiplatelet Therapy ◽  
Peak Velocity ◽  
Coronary Intervention ◽  
Left Ventricular ◽  
Control Group ◽  
Aggregation Rate ◽  
Percutaneous Coronary ◽  
Experimental Group

Objective. To explore the effect of antiplatelet therapy combining aspirin with tirofiban after percutaneous coronary intervention (PCI) on the incidence of re-occlusion of blood vessels and platelet aggregation rate in patients with acute myocardial infarction (AMI). Methods. A total of 104 AMI patients treated in the Department of Cardiovascular Medicine of our hospital from March 2017 to March 2018 were selected for retrospective analysis, and those who met the inclusion criteria were divided into the experimental group (n=52) and the control group (n=52) by sealed envelope randomization. After admission, all patients received the PCI, then the combined therapy of aspirin and tirofiban was given to the patients in the experimental group, and the patients in the control group orally took the clopidogrel. By detecting the values of n-terminal pro-brain natriuretic peptide (NT-proBNP) level, platelet active function indicators, etc. of patients in both groups after treatment, the treatment effect of antiplatelet in AMI patients after PCI with different drugs was analyzed. Results. After treatment, the levels of the maximum platelet aggregation rate (MPAR), CD63, CD62P, MA, NT-proBNP and left ventricular end-diastolic volume (LVEDV) were significantly lower in the experimental group than in the control group (P<0.001), and the R time, K time, CI values, left ventricular ejection fraction (LVEF), the peak velocity of early diastolic wave (peak E)/peak velocity of late diastolic wave (peak A) under mitral valve (E/A) were significantly higher in the experimental group than in the control group (P<0.001), and during follow-up, the incidence rate of re-occlusion of blood vessels was significantly lower in the experimental group than in the control group (P<0.05). Conclusion. The above results indicated that combining aspirin with tirofiban has a better effect than clopidogrel in the antiplatelet therapy for AMI patients after PCI, and therefore it is recommended.

scholarly journals Angiogenesis, fibrinogenesis and presence of synechiae after exeresis of a swine vocal fold mucosal microflap and use of topical mitomycin-C

2010 ◽  
Vol 25 (1) ◽  
pp. 80-85 ◽  
Author(s):  
Vinicius Ribas de Carvalho Duarte Fonseca ◽  
Osvaldo Malafaia ◽  
Jurandir Marcondes Ribas Filho ◽  
Paulo Afonso Nunes Nassif ◽  
Nicolau Gregori Czeczko ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Mitomycin C ◽  
Vocal Fold ◽  
Vocal Folds ◽  
Free Edge ◽  
Control Group ◽  
Collagen Deposition ◽  
Group A ◽  
Immunohistochemical Assessment ◽  
Experimental Group

PURPOSE: To evaluate swine vocal fold healing in a period of 30 days after topical mitomycin-C application. METHODS: Twelve swine underwent exeresis of mucosal flaps from the free edge of the anterior third of both vocal folds with a cold instrument (laryngeal scissors). The animals were divided into two groups: EG (Experimental Group), consisting of 6 animals undergoing topical MMC application (4 mg/dL) on the operated area for 4 minutes; CG (Control Group), 6 animals undergoing topical saline solution application on the operated area for 4 minutes. After 30 days, the animals were sacrificed and the larynges were collected and examined for the presence of synechiae as well as a histological immunohistochemical assessment of immature and mature collagen deposition, number of blood vessels and myofibroblasts. RESULTS: Mature collagen deposition in the EG was 452.12 μm² and 1332.31μm² in the CG; immature collagen deposition was 1511.73μm² in the EG and 1020.61μm² in the CG. The number of myofibroblasts was 1.556 in the EG and 3.583 in the CG. The number of blood vessels was 2.565 in EG and 6.917 in the CG. There were no synechiae in the two studied groups. CONCLUSIONS: There was an increase in immature collagen deposition in the experimental group when compared with the control group. There was a decrease in mature collagen deposition in the experimental group when compared with the control group. There was a decrease in the number of myofibroblasts in the experimental group when compared with the control group. A decrease in blood vessels was observed in the experimental group when compared with the control group. There was no synechia formation in either studied group.

Morphometric analysis of peroxisomes in hepatocytes of alcohol-fed rats

1989 ◽  
Vol 47 ◽  
pp. 1100-1101
Author(s):  
D.C. Dominguez ◽  
J.T. Ellzey
Keyword(s):  
Caloric Intake ◽  
Volume Density ◽  
Controlled Study ◽  
Control Group ◽  
Numerical Density ◽  
Cacodylate Buffer ◽  
Paired Control ◽  
Experimental Group ◽  
Liver Peroxisomes ◽  
Single Lesion

Peroxisomes which participate in 1ipid metabolism have been shown to be altered in several metabolic disorders and toxic conditions. In alcoholic liver disease, the single lesion most frequently found is lipid accumu1ation in hepatocytes. However, the mechanisms for this 1ipid accumu1ation are not clear. The occurrence of modifications of liver peroxisomes due to excess alcohol consumption has not been subjected to a controlled study. We utilized a combination of cytochemica1 and morphometrictechniques to study the size and number of liver peroxisomes in rats fed an alcohol-supplemented diet compared to those of matched-paired control animals.Male Sprague-Daw1ey rats (400-500 g) received a liquid diet. The experimental group (N = 5/group) was fed a diet containing 30% ethanol-derived calories (EDC) and the control group was fed an isocaloric diet to 30% EDC. A pair feeding procedure was employed to control for caloric intake. Small pieces of liver randomly selected, were fixed in 2.3% -glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.2, incubated in a DAB medium and postfixed with. 2% aqueous osmium tetroxide. EM photographs were taken from sections of 3 tissue blocks from each sample (7,200X) with a Zeiss EM10-A (60 kV). With the use of a point counting method and a digital planimeter the volume density (Vv) and numerical density (Nv) were determined.

Values and intergroup perceptions of East Germans and West Germans in 1998 1This research was supported by a grant from the German Research Society (We 1106/3-1).

1999 ◽  
Vol 58 (4) ◽  
pp. 263-272
Author(s):  
Jörg Doll ◽  
Michael Dick
Keyword(s):  
Research Society ◽  
Frame Of Reference ◽  
Control Group ◽  
Perceived Similarity ◽  
The West ◽  
Need For Uniqueness ◽  
German Research ◽  
East German ◽  
Experimental Group ◽  
East West

The studies reported here focus on similarities and dissimilarities between the terminal value hierarchies ( Rokeach, 1973 ) ascribed to different groups ( Schwartz & Struch, 1990 ). In Study 1, n = 65 East Germans and n = 110 West Germans mutually assess the respective ingroup and outgroup. In this intra-German comparison the West Germans, with a mean intraindividual correlation of rho = 0.609, perceive a significantly greater East-West similarity between the group-related value hierarchies than the East Germans, with a mean rho = 0.400. Study 2 gives East German subjects either a Swiss (n = 58) or Polish (n = 59) frame of reference in the comparison between the categories German and East German. Whereas the Swiss frame of reference should arouse a need for uniqueness, the Polish frame of reference should arouse a need for similarity. In accordance with expectations, the Swiss frame of reference significantly reduces the correlative similarity between German and East German from a mean rho = 0.703 in a control group (n = 59) to a mean rho = 0.518 in the experimental group. Contrary to expectations, the Polish frame of reference does not lead to an increase in perceived similarity (mean rho = 0.712).

Rorschach Characteristics in Adolescents with Systemic Lupus Erythematosus

2006 ◽  
Vol 28 (1) ◽  
pp. 100-118 ◽  
Author(s):  
J. Santoantonio ◽  
L. Yazigi ◽  
E. I. Sato
Keyword(s):  
Lupus Erythematosus ◽  
Case Control Study ◽  
Activity Index ◽  
Disease Activity Index ◽  
Female Adolescents ◽  
Control Group ◽  
Intelligence Scale ◽  
The Mean ◽  
Experimental Group ◽  
Control Study

The purpose of this study was to investigate the personality characteristics in adolescents with SLE. The research design is a case-control study by means of the Rorschach Method and the Wechsler Intelligence Scale. Study group: 30 female adolescents with lupus, 12–17 years of age. The SLE Disease Activity Index was administered during the period of psychological evaluation. Control group: 32 nonpatient adolescents were matched for age, sex, and socioeconomic level. In the Wechsler Intelligence Scale the mean IQ of the experimental group was significantly lower than that of the control group (77 and 98, respectively, p < .001). In the Rorschach, the lupus patients showed greater difficulty in interpersonal interactions, although they displayed the resources to process affect and to cope with stressful situations. A positive moderate correlation (p = .069) between the activity index of the disease and the affect constriction proportion of the Rorschach was observed: the higher the SLEDAI score, the lower the capacity to process affect. There is a negative correlation between the activity index of the disease and the IQ (p = .001): with a higher activity index of the disease, less intellectual resources are available.

In a Working Mood?

2008 ◽  
Vol 20 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Leonard Reinecke ◽  
Sabine Trepte
Keyword(s):  
Cognitive Task ◽  
Prior Experience ◽  
Computer Game ◽  
Control Group ◽  
Mood Management ◽  
Management Processes ◽  
Quasi Experimental ◽  
Personal Media ◽  
Experimental Group ◽  
Subsequent Task

Abstract. This quasi-experimental study examined the effects of exposure to a computer game on arousal and subsequent task performance. After inducing a state of low arousal, participants were assigned to experimental or control conditions via self-selection. Members of the experimental group played a computer game for five minutes; subjects in the control group spent the same amount of time awaiting further instructions. Participants who were exposed to the computer game showed significantly higher levels of arousal and performed significantly better on a subsequent cognitive task. The pattern of results was not influenced by the participants' prior experience with the game. The findings indicate that mood-management processes associated with personal media use at the workplace go beyond the alteration of arousal and affect subsequent cognitive performance.

Impact of an educative program on dialysis patients’ knowledge and mental health: controlled before and after program

2018 ◽  
Vol 10 (2) ◽  
pp. 20204
Author(s):  
Laetitia Idier ◽  
Aurélie Untas ◽  
Nicole Rascle ◽  
Michèle Koleck ◽  
Maider Aguirrezabal ◽  
...  
Keyword(s):  
Mental Health ◽  
Depressive Symptoms ◽  
Vascular Access ◽  
Educational Program ◽  
Psychological State ◽  
Control Group ◽  
Dialysis Patients ◽  
Emotional Change ◽  
The Impact ◽  
Experimental Group

Introduction:Psychological impact of Therapeutic Patient Education (TPE) for dialysis patients is rarely evaluated since the focus of many studies is on medical variables (i.e., adherence).Objectives:The aims of this study were: 1) to estimate the impact of a TPE program on knowledge, depression and anxiety, 2) to examine change in knowledge as a mediator of the effects of a TPE program on mental health.Method:This study was conducted in three hemodialysis units and comprised two groups: an experimental group with education and a control group with routine care. The program was based of 5 educative sessions. Knowledge, depressive and anxious symptoms were assessed with self-reported outcomes measured before and 3 months after the program.Results:The sample comprised 125 patients. Knowledge about vascular access and nutrition (p < 0.01) and depressive symptoms increased in the experimental group (p < 0.01). Analysis of mediation showed that changes in knowledge about vascular access were a significant mediator of the effects of the program on depressive symptoms (F = 4.90;p = 0.01).Discussion:Knowledge acquired during an educational program could lead to an emotional change. Improving knowledge often leads to an awareness of the risks that can modify the psychological state of patients by reminding them of their vulnerability. This study shows that it is required to be attentive to the way of transmitting knowledge. It’s necessary adapting this transmission to the needs of patients and promoting the acquisition of psychosocial competence too.Conclusion:This study shows that knowledge acquired during an educational program can lead to an emotional change in the short term. A long-term follow-up of the population should be interesting to observe these emotional effects.

Соматические дисфункции у детей с задержкой речи

2017 ◽  
pp. 61-65
Author(s):  
E. L. Kuznetsova ◽  
L. A. Dultsev ◽  
E. V. Safin
Keyword(s):  
Standard Treatment ◽  
Control Group ◽  
Complex Therapy ◽  
Osteopathic Treatment ◽  
Positive Effect ◽  
Experimental Group

Goal of research - the study aims to examine the osteopathic profi le of children with dysarthria and to develop recommendations for osteopathic correction of somatic dysfunctions in 2-3 year old children presenting this pathology.Materials and methods. 30 2-3 year old children with the symptoms of dysarthria took part in the research. All the children were divided into 2 groups: the control group of 15 children received standard treatment, and the experimental group of 15 children received both standard and osteopathic treatment. The dysarthria severity and the osteopathic profi le were evaluated with account of the number of somatic dysfunctions at global, regional, and local levels.Results. The osteopathic correction was shown to have a positive effect on dysarthria severity. The study established a correlation between the dysarthria severity in children and the number of somatic dysfunctions at the local level.Conclusion. The study suggests using osteopathic correction of somatic dysfunctions in the complex therapy of dysarthria in children.

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