scholarly journals Isolation and Identification of Osmophilic Yeast from unripe Jackfruit (Artocarpus heterophyllus Lamk.)

2019 ◽  
Vol 1 (3) ◽  
pp. 269-279
Author(s):  
Nanik Suhartatik ◽  
Mimin Nurjanah ◽  
Kapti Rahayu Kuswanto
Keyword(s):  
Agar Medium ◽  
Candida Rugosa ◽  
Morphological Characteristics ◽  
Geotrichum Candidum ◽  
Morphological Characterization ◽  
Yeast Growth ◽  
Isolation And Identification ◽  
Physiological Characterization ◽  
Single Colony ◽  
Candida Lambica

Osmophilic yeast are microbes that are able to live on media with high sugar concentrations. Some types of osmophilic yeast can break down organic compounds, for example, starch. This research was aimed to isolate and identify existing osmophilic yeast in unripe jackfruits. In this research, the unripe jackfruit was left rotting and 2.5 g of the sample were taken to be solved in 25 ml 0.85% NaCl and diluted. The GYP (glucose-yeast-peptone) agar medium with the glucose concentration of 30–50% was suitable for osmophilic yeast growth. Isolate purification was performed using the streaking method from single colony. Isolates were identified based on physiological and morphological characteristics. The physiological characterization were carried out by running sugar assimilation test on the tool kit API 20C AUX, while the morphological characterization was carried out by performing macroscopic and microscopic observations. Four isolates were assumed to be Candida lambica, 3 isolates were assumed to be Candida rugosa and two isolates were assumed to be Geotrichum candidum. It was found out that 44.4% of yeast in the unripe jackfruit were Candida lambica, 33.3% was Candida rugosa and 22.2% was Geotrichum candidum. Candida lambica was the most common osmophilic yeast found in the jackfruit.

scholarly journals Isolation and identification of native degrading fungi from Wastewater's pollutants in cardboard recycling industry

2019 ◽  
Author(s):  
Zeinab Hosseini ◽  
Mohammad Taghi Ghaneian ◽  
AbbAsali Jafari Nadoushan ◽  
Mohammad Hassan Ehrampoush ◽  
Seyedeh Mahdieh Namayandeh ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Scientific Literature ◽  
Agar Medium ◽  
Morphological Characteristics ◽  
Fungal Species ◽  
Isolation And Identification ◽  
Recycling Industry ◽  
Native Fungi ◽  
Wastewater Pollutants ◽  
Industry Wastewater

Introduction: By increasing the amount and diversity of bio-environmental wastewater pollutants, it is essential to be controlled and planed the environmental contaminants to eliminate or minimize. Cardboard recycling industry wastewater has the potential of environmental pollution; it is contaminated with various fungal species that may be effective in the decomposition and removal of pollutants. The aim of this study was the isolation and identification of native fungal species that decontaminate organic pollutants in the cardboard recycling industry wastewaters. Methods: In this study, which is a descriptive study, samples were randomly taken from different area of wastewater of cardboard recycling industry. Samples were cultured on Saboraud dextrose agar medium for isolation of probable fungal species, which decomposers of wastewater. The isolated fungal colonies were enumerated and purified based on morphological characteristics and microscopic analyzing as far as possible. Results: The average count of the total number of fungi species in wastewater of cardboard recycling industry was 2.5×102 CFU/ml of these 2.1×101 were saprophytic fungi. The most prevalent fungal species were identificated from the wastewater include Aspergillus niger, Aspergillus flavus, Penicillum, Geotrichum, Mucor, Drechselera, Paecillomyces, Alternaria, Fusarium and Absidia. The highest frequency of fungal species Aspergillus nige, Aspergillus flavus and Penicillum were 30.89%, 22.76% and 14.63% respectively. Conclusion: It seems that native fungi isolated from the cardboard recycling industry wastewater in this study are from fungi that have the potential for bioremediation of wastewater contaminants based on scientific literature and can be used for evaluating of their ability to decompose wastewater from this industry.  

THE PREDOMINANT BACTERIA IN NATURAL ZOOGLOEAL COLONIES: I. ISOLATION AND IDENTIFICATION

1967 ◽  
Vol 13 (12) ◽  
pp. 1671-1682 ◽  
Author(s):  
Richard F. Unz ◽  
Norman C. Dondero
Keyword(s):  
Waste Water ◽  
Single Cell ◽  
Agar Medium ◽  
Isolation And Identification ◽  
Group I ◽  
Yeast Autolysate ◽  
Conventional Methods ◽  
Group Ii ◽  
Produce Acid

Direct, single-cell isolations of bacteria, primarily from natural, branching, waste water zoogloeas, were made by micromanipulation. Isolations were also made by conventional methods. Direct isolates were classified, chiefly with regard to zoogloea formation, into two groups designated group I (zoogloea-forming) and group II (nonzoogloea-forming). Casitone – glycerol – yeast autolysate agar medium was best for the isolation of group I bacteria. Group I isolates reduced nitrate to gas, possessed urease and catalase, and gave positive oxidase reactions. They were generally able to hydrolyze gelatin but, with one exception, did not produce acid from carbohydrates, and none produced H2S, indole, or acetylmethylcarbinol or utilized Koser citrate. Group II strains were usually more diverse on differential tests and could be distinguished from group I strains. Group I strains were characterized as Zoogloea strains and were found to be the predominant bacteria in natural, branching, zoogloeal colonies.

scholarly journals The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods)

Elkawnie ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 222
Author(s):  
Opik Taupik Kurahman ◽  
Astri Yuliawati ◽  
Lusi Haerunnisa ◽  
Ateng Supriyatna ◽  
Tri Cahyanto ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Pathogenic Bacteria ◽  
Morphological Characterization ◽  
Chicken Manure ◽  
Isolation And Identification ◽  
Fish Food ◽  
Commercial Fish ◽  
Fish Intestine ◽  
Commercial Fish Food ◽  
Eating Animal

Abstract: In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar,  Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.

scholarly journals Overview of Agaricus Subrufescens Strains used in the Last 15 Years in Brazil and other Countries and Current Potential Materials for the Future

2021 ◽  
Author(s):  
Diego Cunha Zied ◽  
Wagner G. Vieira Junior ◽  
Douglas M. M. Soares ◽  
Cassius V. Stevani ◽  
Eustáquio S. Dias ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
High Weight ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Agaricus Blazei ◽  
Internal Transcribed Spacer Region ◽  
Commercial Use ◽  
Agaricus Subrufescens ◽  
Current Potential

Abstract The mushroom Agaricus subrufescens has been synonymous with Agaricus blazei and Agaricus brasiliensis during the last decades and there has been much discussion with regards to the origin, distribution, and nomenclature of this mushroom. Therefore, we conducted a genetic and morphological characterization of the mycelium and mushroom of four commercial strains currently cultivated in Brazil (ABL CS7, ABL 18/01, ABL 98/11, and ABL 16/01) together with an assessment of their agronomic behavior and compared these results with those of other strains used during the last 15 years. All the A. subrufescens strains characterized here are phylogenetically related to the Americas/Europe specimens, bearing an internal transcribed spacer region of type A (ABL 16/01) or both types A and B (ABL 18/01, ABL 98/11, and ABL CS7). We did not find any correlation between the morphological characteristics of the mycelial colonies and the agronomic behavior of the strains. Strains ABL 98/11 and ABL 16/01 produced the best yields and morphological characteristics for the mushrooms, indicating their high weight, which enhances the commercialization of the mushroom and justifies their longstanding commercial use over the last 15 years.

scholarly journals Isolation and identification of motile Aeromonas spp.from different sources

2011 ◽  
Vol 5 (3) ◽  
pp. 56-65
Author(s):  
Samira Y. Yousif ◽  
Rasha Abid Ali Al-Khalidi
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Morphological Characterization ◽  
Isolation And Identification ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Susceptibility Tests ◽  
Different Sources

Total of 507 samples (clinical, environmental, food) were collected from different hospitals in Baghdad, water, soil, and different food stuffs. Biochemical and morphological characterization tests showed that seventeen isolates were identified as Aeromonas spp.These were farther characterized as Aeromonas hydrophila 10 isolates, Aeromonas sobria 2 isolates, Aeromonas eucrenophila 3 isolates, one isolate belongs to Aeromonas caviae and another one belongs to Aeromonas schubertii. Antibiotic susceptibility tests of all the isolates towards fifteen antibiotics agents were carried out and results showed that all isolates 100% were resistant to penicillin, ampicillin, ampiclox, 99% were resistant to lincomycin, 76.7% to cephalothin, 52.9% to cefotaxime. All isolates except one isolate of Aeromonas eucrenophila were sensitive to meropenem.

scholarly journals Non-invasive Clinical Measurement of Ocular Rigidity and Comparison to Biomechanical and Morphological Parameters in Glaucomatous and Healthy Subjects

2021 ◽  
Vol 8 ◽  
Author(s):  
Yanhui Ma ◽  
Sayoko E. Moroi ◽  
Cynthia J. Roberts
Keyword(s):  
Healthy Subjects ◽  
High Speed ◽  
Morphological Characteristics ◽  
Volume Ratio ◽  
Pulse Amplitude ◽  
Morphological Characterization ◽  
Dynamic Contour Tonometry ◽  
Stiffness Parameter ◽  
Positive Correlation

Purpose: To assess ocular rigidity using dynamic optical coherence tomography (OCT) videos in glaucomatous and healthy subjects, and to evaluate how ocular rigidity correlates with biomechanical and morphological characteristics of the human eye.Methods: Ocular rigidity was calculated using Friedenwald's empirical equation which estimates the change in intraocular pressure (IOP) produced by volumetric changes of the eye due to choroidal pulsations with each heartbeat. High-speed OCT video was utilized to non-invasively measure changes in choroidal volume through time-series analysis. A control-case study design was based on 23 healthy controls and 6 glaucoma cases. Multiple diagnostic modalities were performed during the same visit including Spectralis OCT for nerve head video, Pascal Dynamic Contour Tonometry for IOP and ocular pulse amplitude (OPA) measurement, Corvis ST for measuring dynamic biomechanical response, and Pentacam for morphological characterization.Results: Combining glaucoma and healthy cohorts (n = 29), there were negative correlations between ocular rigidity and axial length (Pearson R = −0.53, p = 0.003), and between ocular rigidity and anterior chamber volume (R = −0.64, p = 0.0002). There was a stronger positive correlation of ocular rigidity and scleral stiffness (i.e., stiffness parameter at the highest concavity [SP-HC]) (R = 0.62, p = 0.0005) compared to ocular rigidity and corneal stiffness (i.e., stiffness parameter at the first applanation [SP-A1]) (R = 0.41, p = 0.033). In addition, there was a positive correlation between ocular rigidity and the static pressure-volume ratio (P/V ratio) (R = 0.72, p < 0.0001).Conclusions: Ocular rigidity was non-invasively assessed using OCT video and OPA in a clinic setting. The significant correlation of ocular rigidity with biomechanical parameters, SP-HC and P/V ratio, demonstrated the validity of the ocular rigidity measurement. Ocular rigidity is driven to a greater extent by scleral stiffness than corneal stiffness. These in vivo methods offer an important approach to investigate the role of ocular biomechanics in glaucoma.

scholarly journals First Report of Myrothecium roridum Causing Leaf Spot on Zantedeschia aethiopica in China

Plant Disease ◽  
2014 ◽  
Vol 98 (6) ◽  
pp. 854-854 ◽  
Author(s):  
B.-J. Li ◽  
H.-Y. Ben ◽  
Y.-X. Shi ◽  
X.-W. Xie ◽  
A.-L. Chai
Keyword(s):  
Leaf Spot ◽  
Morphological Characteristics ◽  
Its Region ◽  
First Record ◽  
Conidial Suspension ◽  
Isolation And Identification ◽  
Zantedeschia Aethiopica ◽  
Calla Lily ◽  
Initial Symptoms ◽  
Myrothecium Roridum

Zantedeschia aethiopica (L.) Spreng. (calla lily), belonging to family Araceae, is a popular ornamental plant in China. In the summer of 2010, leaves of calla lily with typical symptoms of necrotic lesions were observed in a commercial glasshouse in Beijing, China (116°20′ E, 39°44′ N). The initial symptoms were circular to subcircular, 1 to 3 mm, and dark brown lesions on the leaf lamina. Under high humidity, lesions expanded rapidly to 5 to 10 mm with distinct concentric zones and produced black sporodochia, especially on the backs of leaves. Later, the infected leaves were developing a combination of leaf lesions, yellowing, and falling off; as a result, the aesthetic value of the plant was significantly impacted. Leaf samples were used in pathogen isolation. Symptomatic leaf tissues were cut into small pieces and surface sterilized with 70% ethanol for 30 s and then in 0.1% mercuric chloride solution for 1 to 3 min. After being washed in sterile distilled water three times, the pieces were plated on potato dextrose agar (PDA) and incubated at 25°C in darkness for 7 days (5). Initial colonies of isolates were white, floccose mycelium and developed dark green to black concentric rings that were sporodochia bearing viscid spore masses after incubating 5 days. Conidiophores branched repeatedly. Conidiogenous cells were hyaline, clavate, and 10.0 to 16.0 × 1.4 to 2.0 μm. Conidia were hyaline, cylindrical, both rounded ends, and 6.0 to 8.2 × 1.9 to 2.4 μm. Morphological characteristics of the fungus were consistent with the description of Myrothecium roridum Tode ex Fr. (3,4). To confirm the pathogenicity, three healthy plants of calla lily were inoculated with a conidial suspension (1 × 106 conidia per ml) brushed from a 7-day-old culture of the fungus. Control plants were sprayed with sterile water. The inoculated plants were individual with clear plastic bags and placed in a glass cabinet at 25°C. After 7 days, all inoculated leaves developed symptoms similar to the original samples, but control plants remained disease free. Re-isolation and identification confirmed Koch's postulates. For molecular identification, genomic DNA of a representative isolate (MTL07081001) was extracted by modified CTAB method (1), and the rDNA-ITS region was amplified by using primers ITS1 (5-TCCGTAGGTGAACCTGCGG-3) and ITS4 (5-TCCTCCGCTTATTGATATGC-3). The 465-bp amplicon (GenBank Accession No. KF761293) was 100% identity to the sequence of M. roridum (JF724158.1) from GenBank. M. roridum has an extensive host range, covering 294 host plants (2). To our knowledge, this is the first record of leaf spot caused by M. roridum on calla lily in China. References: (1) F. M. Ausubel et al. Current Protocols in Molecular Biology. John Wiley & Sons Inc, New York, 1994. (2) D. F. Farr and A. Y. Rossman, Fungal Databases. Syst. Mycol. Microbiol. Lab., ARS, USDA. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ , October 2013. (3) M. T. Mmbaga et al. Plant Dis. 94:1266, 2010. (4) Y. X. Zhang et al. Plant Dis. 95:1030, 2011. (5) L. Zhu et al. J. Phytopathol. 161:59, 2013.

scholarly journals Genetic and Morphological Characterization of Mangifera indica L. Growing in Egypt

HortScience ◽  
2018 ◽  
Vol 53 (9) ◽  
pp. 1266-1270 ◽  
Author(s):  
Nader R. Abdelsalam ◽  
Hayssam M. Ali ◽  
Mohamed Z.M. Salem ◽  
Elsayed G. Ibrahem ◽  
Mohamed S. Elshikh
Keyword(s):  
Genetic Diversity ◽  
Mangifera Indica ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Molecular Techniques ◽  
Germplasm Management ◽  
Fruit Crops ◽  
The Family ◽  
High Diversity

Mango (Mangifera indica L.) is a fruit crops belong to the family Anacardiaceae and is the oldest cultivated tree worldwide. Cultivars maintained in Egypt have not been investigated previously. Mango was first brought to Egypt from South Asia. Morphological and molecular techniques were used to identify the genetic diversity within 28 mango cultivars. SSR and EST-SSR were used for optimizing germplasm management of mango cultivars. Significant variations were observed in morphological characteristics and genetic polymorphism, as they ranged from 0.71% to 100%. High diversity was confirmed as a pattern of morphological and genotypes data. Data from the present study may be used to calculate the mango relationship and diversity currently grown in Egypt.

Notes on some lesser known crickets of the tribe Podoscirtini (Podoscirtinae: Gryllidae: Orthoptera) from India 

Zootaxa ◽  
2020 ◽  
Vol 4821 (1) ◽  
pp. 49-70
Author(s):  
ASHOK KUMAR MEENA ◽  
RAJAMANI SWAMINATHAN ◽  
RAJENDRA NAGAR
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Tropical Region ◽  
Two New Species

The present paper deals with a description of two new species of crickets of the subfamily Podoscirtinae (Orthoptera: Gryllidae), Trelleora khasiana sp. nov. and Indotrella maculata sp. nov., from India; besides, some additional morphological characteristics of the already reported species, Indotrella angusta Gorochov, 2003 is also presented. The morphological characterization of these two new species is supported with suitable line diagrams and plates. A checklist of crickets of Podoscirtinae for the Asia-tropical region is included.

scholarly journals MORPHOLOGICAL CHARACTERIZATION OF COFFEE (Coffea arabica L.) LANDRACES AT SEEDLING STAGE COLLECTED FROM GUJI ZONES

2018 ◽  
Vol 30 (2) ◽  
pp. 09-18
Author(s):  
I. D. Alemu ◽  
D. S. Boke
Keyword(s):  
Leaf Area ◽  
Coffea Arabica ◽  
Block Design ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Seedling Stage ◽  
Leaf Width ◽  
Seedling Height ◽  
Node Number ◽  
Randomized Block Design

The present study was aimed to determine genetic diversity of Coffea arabica landraces based on morphological characteristics at seedling stage in Guji zones. Three kebeles and twenty-five farmers’ coffee landraces were selected based on availability of Coffea arabica landraces from each district by the guidance of Developmental Agency in the kebele. Coffea arabica seeds were collected and planted at kercha nursery sub-site in plastic pots arranged on randomized block design. Seedling characteristics were collected from one year old randomly selected four coffee seedlings from each plot. The results showed that there were significant variations (p<0.05) between and within Coffea arabica landraces of the district. The seedling height of Coffea arabica collected from Didiba local was highest (11.25) followed by Sorile local (11.17) whereas Ebala local was the least. Coffea arabica from Diqisa local (5.25) was the highest for leaf height followed by Didiba local (5.13), Harobora (5.00) and Wacufora local (5.00). Similarly, Wacufora local (2.05) and Didiba local (2.53) were the highest in leaf width. The Coffea arabica landrace of Qaqali local (6.25) and Harobora local (5.75) were the highest in number of paired leaves. Correlation between the characteristics of coffee seedling showed that seedling height was high and significantly correlated with leaf width (0.62**), number of paired leaves (0.58**), node number (0.57**) and leaf area (0.64**). Similarly, leaf height was high and significantly correlated with leaf width (0.72**) and leaf area (0.77**). Generally, the increase of leaf height increases the leaf width and leaf area. Existence of morphological variation of Coffea arabica at seedling stage was used to determine Coffea arabica diversity in this study area. Therefore, Coffea arabica landraces having high seedling height, leaf height, number of paired leaves and leaf area should get attention during selection for plantation. Additionally, farmers, conservationists and other concerned bodies should take action to conserve and keep the gene pool of these coffees. As a result, it opens the door for biotechnologists to characterize coffee at molecular level and breeder scan take action on it to release superior coffee varieties.

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