PRELIMINARY STUDY ON THE EFFECT OF WATERMELON JUICE AND MILK SOLUTION ON DE-ADHESION, DEVELOPMENTAL STAGES AND HATCHING RATE OF EGGS AND SURVIVAL OF FRY OF CLARIAS GARIEPINUS

2021 ◽  
Vol 5 (1) ◽  
pp. 314-318
Author(s):  
Abba Salisu ◽  
Bashir Ibrahim Umar ◽  
Amina Umar Usman ◽  
Murtala Namadiana Muhammad ◽  
Mubarak Sani Yakubu ◽  
...  
Keyword(s):  
Survival Rate ◽  
Developmental Stages ◽  
Clarias Gariepinus ◽  
Hatching Rate ◽  
Slight Variation ◽  
Low Oxygen ◽  
Watermelon Juice ◽  
Water Recirculation ◽  
Preliminary Study ◽  
Pathogenic Infection

Sticky layer of Clarias gariepinus eggs cause the clumping of eggs immediately after fertilization, this create low oxygen, and increase pathogenic infection, thereby reducing fertilization and hatching rate hence reduces in mass production of C. gariepinus seed. Although various substances are use in the removal of the sticky layer, no information is available about the use of watermelon. Therefore, the present study, aim in exploring the potential of using watermelon in removing the sticky layer of C. gariepinus to enhance production. Fertilized eggs were treated with watermelon juice (T1) for comparison with 14 g L-1 milk solution (T2 as de-adhesion reference) and water as control (T0 as adhesion reference). The treatments were agitated with aeration for 30 minutes, rinsed with water, and poured on respective netted hatching trough, with water recirculation for incubation at 27 0C for 24 h period. Except for T0, no clumping of eggs was observed in the remaining groups. Slight variation in attaining a developmental stage among eggs was observed in eggs treated with watermelon. Hatching and survival rate in T2 are higher than T1 and T0 (P<0.05). However, watermelon indicated the potential, as it removed the sticky layer while maintaining hatching and survival rate as in the control (T0) (P>0.05), more should be explored before adoption of watermelon for de-adhesion and incubation of C. gariepinus eggs.

scholarly journals Pengaruh penambahan bahan pengencer sperma terhadap fertilitas spermatozoa ikan lele dumbo (Clarias gariepinus)

2014 ◽  
Vol 1 (1) ◽  
pp. 46
Author(s):  
Prama Hartami ◽  
Muhammad Nur ◽  
Eva Ayuzar
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Clarias Gariepinus ◽  
Fertilization Rate ◽  
Coconut Water ◽  
Hatching Rate ◽  
Aceh Province ◽  
Quality Value ◽  
Natural Milk

Penelitian ini dilaksanakan selama 9 hari yang bertempat di Laboratorium Hatcheri dan Teknologi Budidaya Universitas Malikussaleh, Aceh Utara,Propinsi Aceh. Penelitian ini bertujuan untuk mengatahui pengaruh penambahan bahan pengencer sperma terhadap fertilitas spermatozoa ikan lele dumbo dan memperoleh jenis bahan pengencer yang terbaik dalam pemijahan ikan lele dumbo. Penelitian ini di gunakan metode eksperimen laboratorium yang di gunakan pola rancangan acak lengkap (RAL) non faktorial dengan 4 perlakuan 3 ulangan. Bahan uji yang digunakan adalah spermatozoa ikan lele dumbo, Ovaprim, Akuadest, Susu steril, Air kelapa muda, Larutan Infus/ NaCl. Parameter yang di ukur ialah daya fertilitas telur, derajat penetasan telur, kelangsungan hidup benih. Berdasarkan hasil penelitian menunjukkan bahwa penggunaan Aquades sebagai pengencer merupakan bahan yang terbaik dimana fertilitas didapatkan sebesar 98.33 %, derajat penetasan telur sebesar 77.28 %, tingkat kelangsungan hidup larva sebesar 93.44 %. Hasil uji analisis statistik untuk semua parameter uji menunjukkan F hitung > dari F Tabel untuk perlakuan Aquades sebagai bahan pengencer. Sementara itu, hasil pengukuran parameter kualitas air seperti suhu 26,07 – 28,15 0C dan pH air 8,67 – 8,95.This research was done for 9 days in Laboratory of Hatchery and Aquaculture Technology Malikussaleh University, North Aceh, Aceh Province. The aim of this research was to know effect of different sperm solutions for effectiveness fertilization rate of Clarias gariepinus. This research used non factorial completely randomized treatment with three replications on each treatments that using aquadest, natural milk, coconut water and NaCl fisiologis as the sperm solutions. The test observed in this research was fertilization rate, hatching rate, and survival rate. Result showed us that the used of aquadest as the sperm solutions got the best goals which was 98,33 % for ferlilization rate, 77,28 % for hatching rate, and 93,44 % for survival rate. Based on statistical analyze also showed that f count > f table in 99.99 % level of trust for the first treatment with value 29.417 > 7.59. Meanwhile the water quality value that measurable during this research was 26.07 – 28.15 0C and 8.67 – 8.95 for pH.

scholarly journals Induction Of Ovulation Using Ovaprim And Its Impact On Reproductive Parameter Of Clarias gariepinus

2020 ◽  
Vol 3 (2) ◽  
pp. 25
Author(s):  
Paul Morisson Anak Gaung ◽  
Zalina Ismail ◽  
Asilah Abdul Mutalib ◽  
Fazhana Ismail ◽  
Shaibatul ’ Islamiah Che Man ◽  
...  
Keyword(s):  
Survival Rate ◽  
Clarias Gariepinus ◽  
Hatching Rate ◽  
Reproductive Parameter ◽  
Induction Of Ovulation ◽  
Breeding Technique ◽  
Aquaculture Industry ◽  
Experimental Treatments ◽  
Different Doses

Sustainability of the aquaculture industry can be secure through the induce breeding technique. One way that induce breeding can do is to increase the rate of maturation and ovulation in making sure the availability of good quality fish seed all year round. Here, we investigate how Ovaprim hormone impact the reproduction of Clarias gariepinusincluding spawning rate, fecundity, hatching and survival rate. For this purpose, Six broodstocks were divided in three experimental treatments and injected intramuscularly with different doses of Ovaprim hormone of T1: 0.25ml/kg for female and 0.125ml/kg for male, T2: 0.5ml/kg for female and 0.25ml/kg for male and T3: 1.0ml/kg for female and 0.5ml/kg for male. According to our results, broodstock injected with Ovaprim hormone of dose of T3: 1.0ml/kg for female and 0.5ml/kg for male showed higher eggs production and hatching rate compared to other treatment. The results of this study suggest that a dosage of 1.0ml/kg Ovaprim for female and 0.5ml/kg for male have a better effect on the reproductive parameter of Clarias gariepinus.

125 IN VITRO-PRODUCED BOVINE EMBRYOS VITRIFIED IN GLASS CAPILLARIES USING TCM-199 OR DULBECCO'S PHOSPHATE-BUFFERED SALINE AS VITRIFICATION-WARMING MEDIA

2007 ◽  
Vol 19 (1) ◽  
pp. 180 ◽  
Author(s):  
N. Mucci ◽  
G. G. Kaiser ◽  
G. Rios ◽  
R. H. Alberio ◽  
L. B. Ferré ◽  
...  
Keyword(s):  
Survival Rate ◽  
Control Group ◽  
Internal Diameter ◽  
Hatching Rate ◽  
External Diameter ◽  
Low Oxygen ◽  
Embryo Survival ◽  
Glass Capillaries ◽  
Phosphate Buffered Saline

In previous studies (unpublished data) we observed that the replacement of open pulled straws (OPS) with glass capillaries (GC) did not affect the embryo survival rate after vitrification–warming. The aim of this work was to evaluate the post-Cryopreservation survival rate of in vitro-produced bovine blastocysts (B) and expanded blastocysts (eB) using Dulbecco&apos;s phosphate-buffered saline (PBS) or TCM-199 (TCM; Sigma-Aldrich, St Louis, MO, USA) as holding medium during vitrification and warming in glass capillaries. Cumulus–oocyte complexes were in vitro-matured and fertilized as previously described (Mucci et al. 2006 Theriogenology 65, 1551–1562), and cultured in 4 well plates in groups of 50 in 400-�L drops in serum-free CR1aa under low oxygen condition. Grade B1, B2, and eB were selected at Day 7 post-insemination and allocated to 3 groups: vitrification in TCM, vitrification in PBS, and control (without vitrification). Vitrification and warming were performed according to Vajta et al. (1998, Mol. Reprod. Dev. 51, 53–58), replacing OPS with GC (Tecnon Argentina S.A., Buenos Aires, Argentina); 75 mM length, 1.4 mM internal diameter, 1.6 mM external diameter). Briefly, B and eB were incubated in 1.78 M ethylene glycol (EG) and 1.3 M dimethyl sulfoxide (DMSO) in TCM or PBS supplemented with 20% estrous cow serum (ECS) for 3 min. Embryos were then transferred for 25 s to TCM or PBS supplemented with 3.56 M EG, 2.6 M DMSO, 0.5 M sucrose, and 20% ECS (vitrification solution: VS). Loading of embryos (2 per capillary) was performed by touching a 1-�L drop of VS with the capillary. After this, each capillary was immediately submerged into and stored in liquid nitrogen. Warming was performed by placing the capillary tip directly into TCM or PBS supplemented with 0.25 M sucrose for 5 min. Embryos were then transferred to TCM or PBS containing 0.15 M sucrose and 20% ECS for 1 min. After warming, embryos were cultured for 72 h in CR1aa + 5% ECS to evaluate embryo survival (hatching rate). Data was analyzed using the CATMOD procedure (SAS Institute, Inc., Cary, NC, USA). No interaction was found between holding media and embryo stage. Vitrified-warmed embryos had a significantly lower hatching rate compared with the control group (P &lt; 0.05), whereas no differences were found between TCM and PBS. Expanded blastocysts had a higher hatching rate than blastocysts (P &lt; 0.05). In conclusion, TCM can be replaced with PBS for its use in vitrification procedures. This protocol modification allows a simplified use of this technique in field conditions.

scholarly journals MANAJEMEN PEMBENIHAN LELE MUTIARA (Clarias sp.) DENGAN APLIKASI PROBIOTIK DI UNIT PELAYANAN TEKNIS PENGEMBANGAN TEKNOLOGI PERIKANAN BUDIDAYA (UPT PTPB) KEPANJEN, MALANG, JAWA TIMUR

2018 ◽  
Vol 7 (2) ◽  
pp. 84 ◽  
Author(s):  
Regita Ardyanti ◽  
Daruti Dinda Nindarwi ◽  
Luthfiana Aprilianita Sari ◽  
Putri Desi Wulan Sari
Keyword(s):  
Survival Rate ◽  
Clarias Gariepinus ◽  
Hatching Rate

Lele (Clarias sp.) merupakan jenis ikan konsumsi air tawar dengan tubuh memanjang dan kulit licin. Ikan lele MUTIARA adalah strain baru ikan lele Afrika Clarias gariepinus Burchell, 1822 unggul hasil pemuliaan Badan Penelitian Pemuliaan Ikan (BPPI) Sukamandi yang telah dinyatakan lulus pada Penilaian Pelepasan Jenis/Varietas pada tanggal 27 Oktober 2014, dengan nama ikan lele MUTIARA (Mutu Tinggi Tiada Tara). Praktek Kerja Lapang ini dilaksanakan di Unit Pelayanan Teknis Pengembangan Teknologi Perikanan Budidaya Kepanjen, Kabupaten Malang, Jawa Timur pada tanggal 18 Januari - 12 Februari 2016. Praktek Kerja Lapang ini adalah metode partisipasi aktif dengan mengikuti secara langsung kegiatan yang dilakukan dilapangan. Pengambilan data dilakukan dengan cara observasi, wawancara dan partisipasi aktif. Hasil pengamatan menunjukkan bahwa manajemen pembenihan ikan lele mutiara dengan aplikasi probiotik dilakukan dengan pemijahan alami meliputi: persiapan kolam, seleksi induk, pemijahan, fase penetasan telur, pemeliharaan larva dan benih, pemberian pakan, pengendalian hama penyakit dan pembuatan pakan fermentasi. Manajemen Pembenihan Lele Mutiara (Clarias sp.)dilakukan secara alami dengan perbandingan induk jantan dan betina adalah 1 : 1. Jumlah telur yang dihasilkan adalah 71.648 butir, telur yang terbuahi adalah 47.376 butir dan telur yang berhasil menetas adalah 37.310 butir sehingga didapatkan Hatching Rate (HR) sebesar 78,7%. Kelangsungan hidup larva ikan gurami awal pemeliharaan larva berjumlah 37.310 ekor dan jumlah akhir pemeliharaan larva berjumlah 35.000 ekor sehingga didapatkan Survival Rate (SR) sebesar 93,80%.

Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran
Keyword(s):  
Survival Rate ◽  
Experimental Design ◽  
Aromatase Inhibitor ◽  
Fish Larvae ◽  
Clarias Gariepinus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

Optimization of ultraviolet irradiation of Clarias gariepinus (Burchell, 1822) eggs for androgen production

Zygote ◽  
2021 ◽  
pp. 1-6
Author(s):  
Victor Tosin Okomoda ◽  
Haziqah Jumahat Nursyaza ◽  
Ijabo Oga Samuel ◽  
Anuar Hassan ◽  
Abraham Sunday Oladimeji ◽  
...  
Keyword(s):  
Survival Rate ◽  
Uv Irradiation ◽  
Uv Light ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Chromosome Counting ◽  
Complete Inactivation ◽  
Cell Cleavage ◽  
Optimal Protocol ◽  
Prolonged Duration

Summary The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were activated with sperm from diploid C. gariepinus and cold shocked at 5°C for 5 min just moments before cell cleavage. Ploidy analysis was performed using karyotype chromosome counting. The results obtained suggested that the further the distance, the better the hatchability rate, however prolonged duration seemed to significantly reduced hatchability. All treatments with surviving progenies at the end of the study showed evidence of successfully diploid gynogen (2n = 56) induction at different percentages. However, the optimal protocol that gave a moderately high hatchability/survival rate and completely induced gynogens was exposure of the eggs to UV irradiation at 20 cm for 1 min. It was concluded that the distance and duration of UV irradiation affects gynogenetic induction in African catfish C. gariepinus.

scholarly journals ANALISIS KARAKTER REPRODUKSI DAN PERFORMA BENIH HIBRID IKAN NILA PANDU F6 DENGAN IKAN NILA NILASA (Oreochromis niloticus) Analysis of the Character Reproduction and Seed Hybrid Performance 0f Tilapia Fish F6 Pandu with Nilasa Tilapia (Oreochromis niloticus)

2017 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Dio Patria Yustysi ◽  
Fajar Basuki ◽  
Titik Susilowati ◽  
Tristiana Yuniarti
Keyword(s):  
Water Quality ◽  
Growth Rate ◽  
Survival Rate ◽  
Specific Growth Rate ◽  
Oreochromis Niloticus ◽  
Egg Size ◽  
Specific Growth ◽  
Fish Farming ◽  
Hatching Rate ◽  
Feed Conversion

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dari hibridisasi ikan nila Pandu F6 dengan nila Nilasa terhadap karakter reproduksinya dan performa benih yang dihasilkan. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini: perlakuan A (Pandu F6 ♀><Pandu F6 ♂), B (Nilasa ♀><Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), dan D (Nilasa ♀><Pandu F6 ♂). Data yang diamati meliputi fekunditas, daya tetas telur, diameter dan bobot telur, panjang dan bobot larva TL, panjang dan bobot larva lepas kuning telur, kelulushidupan, laju pertumbuhan spesifik, konversi pakan dan kualitas air. Hasil penelitian menunjukan nilai terbaik pada fekunditas sebesar 1191,67 ± 239,45 butir/200 g, daya tetas telur sebesar 80,93 ± 3,90%, kelulushidupan sebesar 81,55 ± 7,54%, laju pertumbuhan spesifik sebesar 7,26 ± 0,25, konversi pakan 0,54 ± 0,02 didapatkan pada perlakuan C, akan tetapi untuk diameter dan bobot telur, panjang dan bobot larva Kuning Telur, dan panjang dan bobot larva lepas kuning telur tidak berbeda nyata. Hasil tersebut menunjukan bahwa hibridisasi (perlakuan C) memberikan pengaruh yang nyata (P < 0,05) terhadap fekunditas, daya tetas telur, kelulushidupan, laju pertumbuhan spesifik, dan konversi pakan tetapi tidak berpengaruh terhadap diameter dan bobot telur, panjang dan bobot larva, dan panjang dan bobot larva lepas kuning telur. Kata kunci: Hibridisasi, Ikan Nila, Karakter Reproduksi, Performa Benih  ABSTRACT This research aims to know the influence of hybridization tilapia fish Pandu F6 with nila Nilasa against the character of the reproduction and seed performance. This research was conducted with the experimental method using Random Design complete (RAL) with 4 treatments and three replicates. The treatments in this study: the treatment A (Pandu F6 F6 ♀ >< Pandu ♂), B (Nilasa ♀>< Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), and D (Nilasa ♀ >< Pandu F6 ♂). The observed data covering fecundity, hatching rate, egg size, yolk sack larva length and weight, length and weight of the egg yolk off larvae, survival rate, specific growth rate, feed conversion rate, and water quality. The results showed the best value on the fecundity of 1191.67±239.45 eggs/200 g, Hatching rate 80.93 ± 3.90%, Survival rate of 81.55 ± 7.54%, specific growth rate of 7.26 ± 0.25, conversion fodder 0.54 ± 0.02 obtained at the treatment C, but for the diameter and weight of egg, larval length and weight TL, and the length and weight of the yolk egg off larvae for each treatment do not differ markedly. The results showed that hybridization (treatment C) gives a real influence (P < 0.05) of fecundity,hatching rate, survival rate but not with the egg size, larva weight and length, and the length and weight of larvae off yolk. Water quality on the spawning, hatching eggs and larvae found on the maintenance of a decent range for tilapia fish farming Keywords: hybridization, tilapia fish, character of the reproduction, seed performance 

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