scholarly journals Anti-inflammatory Activity of Pharmaceutical Gel of Ethanolic Extract from Marine Sponge Xestospongia Sp

2019 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Wa Ode Sitti Zubaydah ◽  
Wahyuni Wahyuni ◽  
Sahidin Sahidin ◽  
Tian Amalia Halik ◽  
Rina Andriani ◽  
...  
Keyword(s):  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control ◽  
Test Method ◽  
Inflammatory Activity ◽  
Extract Concentration ◽  
Dispersion Test ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The aim of this study was to determine the characteristics of the gel formula based on it�s organoleptic properties, pH, viscosity, dispersion and homogenity by stability test carried out using the cycling test method and to determine the anti-inflammatory activity of the Xestospongia Sp. in male white mice (Mus musculus) by creating an artificial edema on the mice left foot induced by 1% ?-carrageenan. The gel formula from the ethanol extract of Xestospongia Sp. sponge was physically stable in terms of its organoleptic observation, homogenity, pH and viscosity test. However, the results that were obtained after dispersion test did not fulfill the requirements. In this study, the gel formula of the ethanol extract of Xestospongia Sp. sponge was administered on the mice left foot by using the variations in extract concentration of 0.02%, 0.03%, and 0.04%, and the gel without extract as a negative control and Galtaren�gel (1% Diclofenac Sodium) as a positive control. The evaluated data were in the form of mice leg edema volume measured based on its percent of inflammation and percent of inflammatory inhibition and observed for 360 minutes. The data were analyzed by using the Kruskal-Wallis test followed by the Mann-Whitney test with a confidence level of 95%. The results of this study showed that the gel formula of ethanol extract of Xestospongia Sp. sponge has an anti-inflammatory effect on each concentration and the formula that has a large anti-inflammatory effect was obtained at extract concentration of 0.04%.

scholarly journals ANTIINFLAMMATION ACTIVITY ETHANOL EXTRACT OF MARBOSI-BOSI LEAVES (Tarenna polycarpa (Miq.) Koord.Ex Valeton) ON WHITE RAT IN CARRAGEENAN INDUCED PAW INFLAMMATION

2018 ◽  
Vol 6 (3) ◽  
pp. 1-4
Author(s):  
Haris Munandarnst ◽  
Marline N
Keyword(s):  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Group Iii ◽  
Rat Paw Edema ◽  
Group I ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Traditionally, (Tarenna polycarpa (Miq.) Koord Ex Valeton as known as marbosi-bosi which commonly found in Sibolga, North Sumatera, Indonesia has been used as antidiabetes, cholesterol, anti-inflammatory and antibacterial. Tarenna species has been found its activities as antimicrobial, anti-oxidant and anti-inflammatory activity. The aim of this study was to investigate the anti inflammatory effect ethanol extract of marbosi-bosi leaves (Tarenna polycarpa (Miq.) Koord Ex Valeton in terms of decreased edema volume of male white rat in 1% carrageenan-induced and also to determine the effective dose of extract to decrease the volume of rat paw edema Ethanol extract of marbosi-bosi (Tarenna polycarpa (Miq.) Koord Ex Valeton was obtained by maceration. The antiinflammatory activity test was divided into 5 groups. The Group I (negative control) was given CMC 0.5%, Group II (positive control) was given diclofenac sodium 2,25 mg / kg BW, while Group III, IV and V were  given marbosi-bosi leaf extract at a dose of 50, 100 and 200 mg/kgBW respectively.  Each rat was induced by 1% carrageenan subplantar injection. Examination of antiinflammatory effect was measured by using digital plethysmometer at minute of 30 to minute of 360. The data were analyzed statistically using ANOVA (analysis of variance).The results showed that negative control did not show anti-inflammatory effect had significant differences with other treatment groups. In conclusion, ethanol extract marbosi-bosi (Tarenna polycarpa (Miq.) Koord Ex Valeton has an effective anti-inflammatory activity at a dose of 100 mg / kgBW.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF MARINE SPONGE CALLYSPONGIA SP. AND ITS ACUTE TOXICITY

2019 ◽  
pp. 97-100
Author(s):  
ADRYAN FRISTIOHADY ◽  
WAHYUNI WAHYUNI ◽  
FADHLIYAH MALIK ◽  
LA ODE MUHAMMAD JULIAN PURNAMA ◽  
BARU SADARUN ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Inflammatory Activity ◽  
Toxicity Assay ◽  
Membrane Method ◽  
Activity Test ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Objective: This study aims to investigate the anti-inflammatory effect of the ethanolic extract of Callyspongia sp. using stabilization of the human red blood cell (HRBC) membrane method and its acute toxicity using brine shrimp lethality test (BSLT) method. Methods: Callyspongia sp. was macerated with 96% ethanol. Extract characterized and screened for the secondary metabolite. Anti-inflammatory activity by stabilization of the HRBC membrane method with a varied dose of 50 ppm; 100 ppm; 200 ppm; 400 ppm; 800 ppm; 1600 ppm; and 3200 ppm. Solutions observed using a photometer to describing stability and ability in preventing membranes hemolytic and statistically analyzed using SPSS. Acute toxicity carried out by the BSLT method and analyzed using Minitab®ver. 17.2.1. Results: The phytochemical screening was indicating that Callyspongia sp. contains flavonoid, alkaloid, and terpenoid. The results of the anti-inflammatory activity test showed that the percentage value of stability and hemolysis of extracts with doses of 50, 100, 200, 400, 800, 1600, and 3200 ppm were 55% and 45%, 63% and 37%, 70% and 30%, 74% and 26%, 80% and 20%, 87% and 13%, and 97% and 3%, respectively. It showed that extract of sponge Callyspongia sp. in all varied dose has activity in stabilizing the HRBC membrane thus can be potential as an anti-inflammatory. The results of acute toxicity assay showed that the value of LC50 was 1281.45 μg/ml and categorized as nontoxic to Artemia salina Leach. Conclusion: Various concentrations of Callyspongia sp. effective as an anti-inflammatory in stabilizing HRBC, and categorized as safe.

scholarly journals Anti-Inflammatory Activity of Ethanol And Fraction of Buni Leaves (Antidesma Bunius L.) on White Rat In Carrageenan Induced Paw Inflammation

1970 ◽  
Vol 7 (5) ◽  
pp. 1-5
Author(s):  
Lintang Kautsar ◽  
Panal Sitorus ◽  
Aminah Dalimunthe
Keyword(s):  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Group Iii ◽  
Group I ◽  
Anti Inflammatory ◽  
Rat Paw

Objectives: The purpose of this study was to investigate the anti-inflammatory effect ethanol extract and fraction of buni leaves (Antidesma bunius L.) in term of decreased edema volume of male white rat in 1% carrageenan-induced and also to determine the effective dose Design: The design of this study was experimental, where the extraction and fraction of buni leaves were tested for inflammation inhibition value ​​in carrageenan-induced white rats.The inflammatory activity test was divided 6 groups. The Group I (negative control) was given CMC 0.5%, Group II (positive control) was given diclofenac sodium 2,25 mg/kg bw (body weight), while Group III, IV, V and VI were given buni leaves extract and fraction at a dose of 100, 200 and 400 mg/kgbw respectively Interventions: The variable that was intervened in this study was the concentration of extract used Main outcome measure: The main measurement results in this study were to know the extracts and fractions which are capable inhibitor volume of edema in the carrageenan-induced rat paw Results: the anti-inflammatory effects from buni leaves exhibited the most effective activity to reduce edema in the rat paw i.e. EEDB 200 mg / kg bw and FEADB 200 mg / kg bw showing the same value of against positive control Conclusion: ethanol extract and ethyl acetate fraction buni leaves(Antidesma bunius L.) has an effective anti-inflammatory activity at a dose of 200 mg/kg bw.

scholarly journals Anti-Inflammatory Activity of Marine Sponge Aaptos sp. to the Plasma Interleukin-1β Level in Wistar Male Rats

2019 ◽  
Vol 4 (2) ◽  
pp. 35
Author(s):  
Adryan Fristiyohadi ◽  
Wahyuni Wahyuni ◽  
Wa OIL. Kalimin ◽  
La OMJ. Permana ◽  
Saripuddin Saripuddin ◽  
...  
Keyword(s):  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Negative Control ◽  
The Body ◽  
Inflammatory Activity ◽  
Male Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Wistar Male Rats

Inflammation is the response of the body to injury and infection characterized by swelling, heat, pain, and redness. This study aimed to investigate the anti-inflammatory effect of Aaptos sp. ethanolic extract to plasma interleukin (IL)-1β level of Wistar male rats. Aaptos sp. was macerated with 96% ethanol for 3 x 24 hours. Inflammation was induced with administration of 1% carrageenan intraplantarly. Animals were divided into 5 treatment groups, i.e., positive control  (diclofenac sodium 3598 ppm); Aaptos sp extract 50 ppm; Aaptos sp extract 100 ppm Aaptos sp extract 200 ppm; and negative control (0.5% Na CMC). After 1 hour, blood was collected and assayed using enzyme-linked immunosorbent assay (ELISA) kit. The results showed that plasma IL-1β levels of animals were decreased by Aaptos sp ethanolic extract. The administration of 50 ppm of extract showed no significant difference (p>0.05)  in IL-1β level in first and second hour measurement, but indicated a statistically significant decrease after three hour (p<0.05). The administration of 100 ppm of extract showed no significant difference (p>0.05) in every hour. Significant reduction was observed in the administration of 200 ppm of extract, but the elevation of IL-1β levels was also observed at third hour measurement. In conclusion, ethanolic extract of Aaptos sp. had anti-inflammatory activity and its effective dose was 50 ppm.   

scholarly journals AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL 70% DAUN ASHITABA (Angelica keiskei) SECARA IN VIVO DENGAN PENGINDUKSI KARAGENAN

2019 ◽  
Author(s):  
Haka As'ada ◽  
Yardi Saibi ◽  
Hendri Aldrat
Keyword(s):  
Dose Range ◽  
White Male ◽  
Ethanol Extract ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Male Rat ◽  
Control Group ◽  
Angelica Keiskei ◽  
Anti Inflammatory

Ashitaba leaves (Angelica keiskei) or also known as tommorow's leaf is plant that known to have various health benefit, one of them is as an anti-inflammatory activity. The anti-inflammatory activity of ashitaba leaves has been known through in vitro assays. This study aims to determine the anti-inflammatory activity of 70% ethanol extract of ashitaba leaves through in vivo assay. Anti-inflammatory activity was performed on white male rat of Sprague dawley strain with induction method of edema on rat's foot using 1% carrageenan 0.2 ml. Rats were divided into 5 groups. The negative control group was given a 0.5% Na-CMC suspension, a positive control group was given sodium diclofenac suspension of 5.14 mg / kgBW, and the test group was given 70% ethanol extract of ashitaba leaves at a dose of 1000; 2000; and 4000 mg / kgBW suspended in 0.5% Na-CMC. The results showed that in that dose range the 70% ethanol extract of ashitaba leaves had anti-inflammatory activity that did not depend on the dose. Percentage of edema of 70% ethanol extract of ashitaba leaves dose 1000; 2000; 4000 mg / kgBB was significantly different with negative control (p ≤ 0,05) and had percentage of edema inhibition respectively 83,95%, 79,01%, and 80,25%. The results of this study showed that 70% ethanol extract of ashitaba leaves have anti-inflammatory activity. Keywords: Ashitaba, Angelica keiskei, tommorow's leaf, anti-inflammatory, carrageenan.

scholarly journals Sesquiterpenoids and Their Anti-Inflammatory Activity: Evaluation of Ainsliaea yunnanensis

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1701
Author(s):  
Jinjie Li ◽  
Xiuting Li ◽  
Xin Wang ◽  
Xiangjian Zhong ◽  
Linlin Ji ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
Ethanolic Extract ◽  
The Self ◽  
Inflammatory Activity ◽  
Spectroscopic Methods ◽  
Activity Evaluation ◽  
Caspase 1 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Four new sesquiterpenoids (1–4) and six known sesquiterpenoids (5–10), were isolated from the EtOAc phase of the ethanolic extract of Ainsliaea yunnanensis. Their structures were established by spectroscopic methods, including 1-D, 2-D NMR and HPLC-MS. All compounds were tested for their anti-inflammatory effect by the inhibition of the activity of NLRP3 inflammasome by blocking the self-slicing of pro-caspase-1, which is induced by nigericin, then the secretion of mature IL-1β, mediated by caspase-1, was suppressed. Unfortunately none of the compounds showed an anti-inflammatory effect.

scholarly journals ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT

2018 ◽  
Vol 11 (4) ◽  
pp. 81
Author(s):  
Hanafis Sastra Winata ◽  
Rosidah Rosidah ◽  
Panal Sitorus
Keyword(s):  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Garcinia Xanthochymus ◽  
Anti Inflammatory

 Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.

scholarly journals Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Clerodendrum inerme (L.) Gaertn.

2015 ◽  
Vol 17 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Dipa Khanam ◽  
Debashish Deb ◽  
Shrabanti Dev ◽  
Masum Shahriar ◽  
Asish K Das ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Skin Diseases ◽  
Diclofenac Sodium ◽  
Latency Period ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Edema Formation ◽  
Standard Drug ◽  
Anti Inflammatory

Clerodendrum inerme (L.) Gaertn. (Verbenaceae) is very popular among the traditional practitioners in Bangladesh for the treatment of local pain and inflammation, skin diseases, topical burns etc. However, so far no scientific study has been carried out which may support its uses in traditional medicine. In the present study, we evaluated the possible analgesic and anti-inflammatory activities of the ethanol extract of C. inerme for the first time. Analgesic activity was assessed by using acetic acid-induced writhing and heat-induced pain in mice and anti-inflammatory activity using xylene-induced ear edema in mice at the doses of 250 and 500 mg/kg body weight. The extract significantly (P< 0.05) attenuated the acetic acid-induced writhing with the highest activity being observed at 500 mg/kg b.w. (45.83%) comparable to that of diclofenac sodium (57.64%), the standard drug. A significant dosedependent increase (P< 0.05) of the latency period was also observed in hot plate method. In the xylene-induced inflammation assay, the extract showed significant (P<0.05) and dose dependant inhibitory effect on the edema formation. These findings indicate that the extract has significant analgesic and anti-inflammatory activities which support the folkloric claim of this plant and thus it has a great potential as source of natural products-derived drug. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22317 Bangladesh Pharmaceutical Journal 17(1): 62-66, 2014

scholarly journals Evaluation Patch of Rhizoma Extract Kencur (Kaempferia galanga L.) as Anti-Inflammatory with Enhancer

2019 ◽  
Vol 6 (2) ◽  
pp. 59
Author(s):  
Hesti Riasari ◽  
Revika Rachmaniar ◽  
Sri Wahyuni
Keyword(s):  
Ethyl Acetate ◽  
Propylene Glycol ◽  
Ethyl Acetate Extract ◽  
Diclofenac Sodium ◽  
Pharmaceutical Preparations ◽  
Ethanol Extract ◽  
Negative Control ◽  
Hexane Extract ◽  
Kaempferia Galanga ◽  
Anti Inflammatory

Kencur (Kaempferia galanga L.) is a family of Zingiberaceae. Several studies have shown that kencur can help reduce inflammation because kencur is known to contain anti-inflammatory compounds, namely marker compounds from flavonoids, kaempferol. For the development of pharmaceutical preparations, research on anti-inflammatory plasters containing 96% ethanol extract, n-hexane extract, ethyl acetate extract and 70% ethanol extract from ginger rhizome with the addition of penetration enhancer (enhancer), namely propylene glycol. This anti-inflammatory plaster was tested for its activity in 5 groups of Wistar strain rat feet which had been induced 1% carrageenan (negative control); positive control (diclofenac sodium), ethanol96% extract, n-hexane extract, ethyl acetate extract and 70% ethanol extract from kencur rhizome and compared with plaster of kencur rhizome ethanol extract without enhancer. The results showed the effect of adding enhancers 30 minutes after administration. 96% ethanol extract and ethyl acetate extract had reduced inflammation by 79.99% in rat test animals compared to plaster ethanol extract of rhizome kencur without the addition of enhancers. Keywords :  Kaempferia galanga. L., patch, anti-inflammatory, enhancer, propylene glycol

scholarly journals Sorbaria kirilowii Ethanol Extract Exerts Anti-Inflammatory Effects In Vitro and In Vivo by Targeting Src/Nuclear Factor (NF)-κB

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 741 ◽  
Author(s):  
Jiwon Jang ◽  
Jong Sub Lee ◽  
Young-Jin Jang ◽  
Eui Su Choung ◽  
Wan Yi Li ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Ex Vivo ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
No Production ◽  
Nuclear Factor ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Inflammation is a fundamental process for defending against foreign antigens that involves various transcriptional regulatory processes as well as molecular signaling pathways. Despite its protective roles in the human body, the activation of inflammation may also convey various diseases including autoimmune disease and cancer. Sorbaria kirilowii is a plant originating from Asia, with no anti-inflammatory activity reported. In this paper, we discovered an anti-inflammatory effect of S. kirilowii ethanol extract (Sk-EE) both in vivo and in vitro. In vitro effects of Sk-EE were determined with lipopolysaccharide (LPS)-stimulated RAW264.7 cells, while ex vivo analysis was performed using peritoneal macrophages of thioglycollate (TG)-induced mice. Sk-EE significantly reduced the nitric oxide (NO) production of induced macrophages and inhibited the expression of inflammation-related cytokines and the activation of transcription factors. Moreover, treatment with Sk-EE also decreased the activation of proteins involved in nuclear factor (NF)-κB signaling cascade; among them, Src was a prime target of Sk-EE. For in vivo assessment of the anti-inflammatory effect of Sk-EE, HCl/EtOH was given by the oral route to mice for gastritis induction. Sk-EE injection dose-dependently reduced the inflammatory lesion area of the stomach in gastritis-induced mice. Taking these results together, Sk-EE exerts its anti-inflammatory activity by regulating intracellular NF-κB signaling pathways and also shows an authentic effect on reducing gastric inflammation.

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