scholarly journals Determination of antibacterial residues in raw cattle beef in some selective areas of Mymensingh District during Eid-ul-Adha

2021 ◽  
Vol 18 (2) ◽  
Author(s):  
M. Comruzzaman ◽  
A. K. M. H. Kabir ◽  
A. Nahar ◽  
N. Hassan ◽  
M. M. Alam
Keyword(s):  
Public Health ◽  
High Performance ◽  
Health Concern ◽  
Animal Origin ◽  
Withdrawal Period ◽  
Drug Residue ◽  
The Mean ◽  
Public Health Hazards ◽  
Antibiotic Residue

Background: Antibiotic residue in foods of animal origin is a growing public health concern in recent time. Indiscriminate use of antibacterials in food animal production and ignoring proper withdrawal period may lead to drug residue in milk, beef and eggs. The present study was conducted to detect antibacterial residues in raw cattle beef samples. Methods: One hundred cattle beef samples were collected from four Upazillas of Mymensingh named Gofargaon, Fulpur, Dhobaura and Bhaluka, Bangladesh during the period of Eid-ul-adha in 2018. The residue of two most commonly used antibacterials named oxytetracycline (OTC) and sulphadimidin (SDI) were quantified by using High Performance Liquid Chromatography (HPLC). Results The mean concentration of OTC and SDI residues in raw beef samples were 0.145 µg/ml and 0.094 µg/ml and the concentration ranged from 0.104-0.435 µg/ml and 0.083-0.357 µg/ml, respectively. About 59% and 43% beef samples contained some levels of OTC and SDI residue, respectively. Importantly, 19% and 12% beef samples exceeded maximum residue limit (MRL) for OTC and SDI, respectively. Conclusions: The results indicate that drug residue is common in raw beef samples and a good proportion of beef samples exceeded MRL. Farmer’s education to ensure rational use of antibacterials and to follow withdrawal period may help control this public health hazards in Bangladesh context.

scholarly journals Simultaneous HPLC Determination of Three Bioactive Alkaloids in the Asian Medicinal Plant Stephania rotunda

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Sothavireak Bory ◽  
Sok-Siya Bun ◽  
Béatrice Baghdikian ◽  
Fathi Mabrouki ◽  
Sun Kaing Cheng ◽  
...  
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Solvent System ◽  
Hplc Method ◽  
Photodiode Array Detection ◽  
Linear Behavior ◽  
Photodiode Array ◽  
Array Detection ◽  
The Mean

A reliable high-performance liquid chromatography (HPLC) method coupled with photodiode array detection has been developed and validated for the determination of three major alkaloids: cepharanthine, tetrahydropalmatine and xylopinine in Stephania rotunda Lour. (Menispermaceae) collected in Cambodia. The chromatographic separation was carried out on a Symmetry C8 column (250 mm x 4.6 mm, 5 μm, Waters), with an isocratic solvent system of 25 mM potassium phosphate buffer (pH 3.5) – acetonitrile. UV detection was performed at 282 nm. Good linear behavior over the investigated concentration ranges was observed with values of r2>0.9964 for all the analytes. The method was reproducible with intra- and inter-day variations of less than 3.91%. The mean recoveries of the analytes ranged from 95.7 to 104.6%. The proposed method was linear, accurate, precise and specific. The validated method was successfully applied to quantify the three alkaloids in various parts of Stephania rotunda and in tubers collected from different Cambodian regions. The results indicated that the developed HPLC method could be used for the quality control of S. rotunda.

scholarly journals A Green HPLC Method for Determination of Nine Sulfonamides in Milk and Beef, and Its Greenness Assessment with Analytical Eco-Scale and Greenness Profile

2020 ◽  
Vol 103 (4) ◽  
pp. 1181-1189
Author(s):  
Xiaoyun Duan ◽  
Xiaofeng Liu ◽  
Yue Dong ◽  
Jing Yang ◽  
Jing Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Animal Origin ◽  
Chromatography Method ◽  
Limit Of Quantitation ◽  
Liquid Chromatography Method ◽  
Animal Origin Foods

Abstract Background Sulfonamides have been widely used in the prevention and clinical treatment of bacterial diseases in livestock and poultry. The use of sulfonamides increases the risk of veterinary drug residues in animal derived foods. The traditional reversed phase liquid chromatography methods for sulfonamides residues detection in animal derived foods have the problem of high consumption of organic solvents. Objective The aim of this study was to establish a green high-performance liquid chromatography method for the detection of sulfonamides residues in different animal-origin foods. Method The sample extraction solutions were purified by the Agela Cleanert PEP-2 cartridge and analyzed by the high-performance liquid chromatography method using ethanol as the green alternative solvent. Results The proposed method was validated in terms of linear range (20–1000 μg/kg), limit of detection (3.0–12.3 μg/kg), limit of quantitation (10–43 μg/kg), accuracy (80.7–101.3%), and repeatability and reproducibility (RSD <5.9% and RSD <8.5% respectively). Conclusions The proposed method is an environmentally friendly, sensitive and reliable high-performance liquid chromatography method for simultaneous determination of sulfonamide residues in animal-origin foods. Highlights In this work, we firstly developed a green high-performance liquid chromatography method for simultaneous determination of the residues of nine sulfonamides in milk and beef with ethanol as the green alternative solvent.

scholarly journals Determination of Ethoxyquin by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to the Survey of Residues in Food Products of Animal Origin

2010 ◽  
Vol 93 (1) ◽  
pp. 277-283 ◽  
Author(s):  
Yoichi Aoki ◽  
Akira Kotani ◽  
Naomi Miyazawa ◽  
Kazunari Uchida ◽  
Yu Igarashi ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
High Performance ◽  
Calculated Data ◽  
Food Products ◽  
Animal Origin ◽  
Tokyo Metropolitan Area ◽  
Chicken Fat ◽  
Standard Solution ◽  
Residue Levels

Abstract An analytical method using HPLC with fluorescence detection (HPLC-FL) has been developed and applied for the survey of residue levels of ethoxyquin in a variety of food products of animal origin. HPLC was performed using a silica octadecylsilane column, butylhydroxytolueneacetonitrilewater (0.05 + 800 + 200, v/v/v) mobile phase, and detection at excitation and emission wavelengths of 370 and 415 nm, respectively. HPLC/MS was used to confirm whether a chromatographic peak was ethoxyquin. The LOQ of the foods was 0.01 µg/g, except for pig fat and cow's milk, and the RSD (n = 6) at 0.1 µg/mL of the standard solution was 1.12. The accuracy of the calculated data of the standard solution was within the range of 94.0 to 101.2. Recoveries of ethoxyquin from the food products of cattle, pigs, chickens, and salmon were more than 71.0 with an RSD of <9.3, except for chicken liver at different concentration levels, including the lower LOQ, the maximum residue limit (MRL), and in some tissues, twice the MRL. Residue levels of ethoxyquin in 33 commercially available food products of animal origin that were purchased on the west side of the Tokyo metropolitan area were surveyed. Contents of ethoxyquin residues in three chicken fat samples by the HPLC-FL method were 0.08, 0.03, and 0.04 µg/g, all less than the MRL (5 µg/g).

Determination of β -Sitosterol with Chemical Course and Material Applications in Jatropha Seed Oil by High Performance Liquid Chromatography

2012 ◽  
Vol 577 ◽  
pp. 69-72 ◽  
Author(s):  
Shu Yu Liu ◽  
Anaerguli Maihemuti
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Seed Oil ◽  
Reversed Phase ◽  
Analytical Recovery ◽  
Relative Standard ◽  
The Mean ◽  
Jatropha Seed

A simple and rapid high performance liquid chromatography (HPLC) assay was developed to identify and measure theβ-sitosterol with chemical course and material applications in jatropha seed oil. The stigmasterol was isolated with a good selectivity by HPLC employing reversed phase C18 columns. The components were separated by mobile phase of methanol-water (99/1, v/v) and detected at 205nm. The quantitation of the stigmasterol was reproducible and the method relative standard deviation is 1.1%. The mean analytical recovery was 96.2%.

scholarly journals Multiresidue method for the simultaneous analysis of antibiotics and mycotoxins in feeds by ultra-high performance liquid chromatography coupled to tandem mass spectrometry

2021 ◽  
Author(s):  
Ü.İ. Konak ◽  
H.A. Yatmaz ◽  
Ş. Nilüfer ◽  
T. Erkaymaz ◽  
M. Certel
Keyword(s):  
High Performance ◽  
Safety Issue ◽  
Animal Health ◽  
Animal Origin ◽  
Limit Of Quantification ◽  
Multiresidue Method ◽  
Animal Feeds ◽  
Relative Standard ◽  
Feed Samples

AbstractResidues in animal feeds and foods of animal origin have been important safety issue concerning both human and animal health. A multiresidue method for determination of eight mycotoxins and ten antibiotics was developed and validated in animal feeds by using QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by UHPLC-MS/MS. Optimisation of UHPLC-MS/MS parameters was performed to achieve good separation and resolution. The method was validated according to the European Commission Decision 2002/657/EC. Matrix matched calibration curves showed good r2 (≥0.995) values, and limit of quantification (LOQ) values varied between 1.2 and 5.2 μg kg−1. Average recoveries ranged from 60 to 102% with relative standard deviations of 2.2 and 15.6% for all type of feed samples except for tetracyclines, lincomycin, tylosin, ochratoxin A, and fumonisin (B1 and B2).

scholarly journals Different perspectives associated with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs: Bangladesh perspectives

2018 ◽  
Vol 2 (2) ◽  
pp. 71-78
Author(s):  
Md Zobidul Kabir ◽  
Alam Khan ◽  
Md Jahanur Rahman ◽  
Moizur Rahman
Keyword(s):  
Monitoring System ◽  
Quantitative Methods ◽  
Public Awareness ◽  
Veterinary Drugs ◽  
Bank Loan ◽  
Health Concern ◽  
Animal Origin ◽  
Withdrawal Period ◽  
Capacity Enhancement ◽  
Substantial Role

Veterinary drugs have proven to be one of the most valuable tools in preserving animal health. With an increase in the use and availability of veterinary drugs, residues of veterinary drugs in animal origin food has become a public health concern and has received much attention from government agencies, consumer level, media and other concerned public interest groups. The objectives of the study were to identify the perspectives associated with the poor management for maintenance of withdrawal period of veterinary drugs in Bangladesh so that the development of relevant strategies for maintenance of withdrawal period of veterinary drugs could be achieved in context of environmental realities in Bangladesh. The survey was interpretive in nature using quantitative methods. A non-experimental and descriptive research design was used to conduct this study. The survey study was carried out at seven divisions (i.e. Rangpur, Rajshahi, Khulna, Dhaka, Sylhet, Barishal and Chattogram) in Bangladesh during July 2012 to June 2018 by using close ended questionnaires among randomly selected respondents. Survey instruments were also designed to capture the perspectives of two different groups of government veterinarians: Group 1– Veterinary Surgeons of DLS, Bangladesh and Group 2 –Upazila Livestock Officers of DLS, Bangladesh. The findings indicated the knowledge, beliefs and perceptions of respondents. The research findings divulge that different perspectives/dimensions associated with economic loss, compensation support, awareness, ethical motivation, monitoring system, proper legislation and guideline, cooperative and group livestock production as well as marketing system had „moderate to substantial role‟ with the management for maintenance of withdrawal period of veterinary drugs in Bangladesh. Besides these, findings also indicated that other perspectives/dimensions associated with allocation of subsidies and Bank loan with low interest, opportunity of livestock/ poultry insurance services, capacity enhancement for monitoring system, availability of technology to producers level, extension work with motivation, magistracy empowerment for veterinarians, legal influence, capacity enhancement of DLS staff, building of public awareness on drug residue problem and availability of effective equipment as well as modern analytical techniques for diagnostic analysis of chemical/drug residues also had „moderate to substantial role‟ with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs. Findings indicated that the two groups of veterinarians possessed near to similar/same perceptions across different dimensions/perspectives associated with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs in Bangladesh and that no significant differences in perspectives existed (p> .05). Findings of this study could be implemented for formulation of effective policy as well as guideline with the development of relevant strategies for maintenance of withdrawal period of veterinary drugs in context of environmental realities in Bangladesh. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 71-78

scholarly journals LC-MS/MS METHOD FOR THE SIMULTANEOUS DETERMINATION OF CEFEPIME AND TAZOBACTAM IN DOG PLASMA

2018 ◽  
Vol 2 (1) ◽  
pp. 36-45
Author(s):  
Kiran R. Patil ◽  
Sukanta K. Naik ◽  
Vineet S. Zope ◽  
Rajesh P. Chavan ◽  
Ravindra D. Yeole
Keyword(s):  
High Performance ◽  
Kinetic Studies ◽  
Hplc Method ◽  
Tandem Mass ◽  
Isocratic Elution ◽  
Simultaneous Quantification ◽  
Dog Plasma ◽  
Tandem Mass Spectrometric ◽  
The Mean

A high performance liquid chromatography (HPLC) method with tandem mass spectrometric detection (MS/MS) has been developed and validated for the simultaneous quantification of cefepime and tazobactam in dog plasma. The method was developed on amide column with isocratic elution. The developed method is simple and economic in terms of sample preparation. The method is specific, sensitive, accurate, precise and robust. The method was successfully applied for pre-clinical pharmaco-kinetic studies in dogs. The Tmax was found to be 0.5 hr, the mean Cmax and AUC(0-12) displayed dose proportionate response.

scholarly journals Quality assessment of selected co-trimoxazole suspension brands marketed in Nairobi County, Kenya

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257625
Author(s):  
Beatrice Njeri Irungu ◽  
Lilian C. Koech ◽  
Joyce M. Ondicho ◽  
Lucia K. Keter
Keyword(s):  
Public Health ◽  
United States ◽  
High Performance ◽  
Opportunistic Infections ◽  
Poor Quality ◽  
United States Pharmacopeia ◽  
Health Concern ◽  
Fixed Dose ◽  
States Pharmacopeia

Introduction Quality of medicines in both developed and developing countries is sometimes compromised due to infiltration of counterfeit, substandard or degraded medicines into the markets. It is a public health concern as poor quality medicines endanger public health where patients are exposed to chemical toxins and/or sub-therapeutic doses. This could lead to reduced treatment efficacy and promote development of drug resistance. Co-trimoxazole, a fixed dose combination of sulfamethoxazole and trimethoprim, is a broad spectrum for bacterial diseases and is also used as a prophylaxis for opportunistic infections in HIV infected individuals. This study evaluated quality of selected co-trimoxazole suspension brands marketed in Nairobi County, Kenya. Methods A total of 106 samples were collected, categorized into 15 brands and evaluated for active pharmaceutical ingredient content (API) and pH following United States Pharmacopeia. Assay for API was conducted using High Performance Liquid Chromatography. Results were compared with pharmacopeia references. Visual examination of labels and confirmation of retention status of the brands with Pharmacy and Poisons Board retention register was carried out. Results The samples were primarily of local origin (86.7%). On October 23, 2019, retention status of six of the fifteen brands documented were no longer listed in the Pharmacy and Poisons Board retention register. Of the 106 samples tested 70.6% and 86.8% were compliant with United States Pharmacopeia (USP) specifications for pH and API respectively while 84.0% adhered to packaging and labelling requirements. Conclusion This study has demonstrated that majority of co-trimoxazole suspensions tested were compliant with USP requirements. Additionally, it has provided evidence of poor quality co-trimoxazole medicines that could compromise treatment of infectious diseases in children. This emphasizes the need for regular quality assurance tests to ensure only quality medicines are in the market.

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