scholarly journals The Effect of Multistage Ammoniation on Fiber Fraction and Digestibility of Maize Stover in vitro

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
K. Dakaew ◽  
A. Abrar ◽  
A. Cherdthong
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Total Volatile ◽  
Maize Stover ◽  
Dry Matter Digestibility ◽  
Fiber Fraction ◽  
Total Volatile Fatty Acid

The main objective of this experiment was to assess the effect of multistage ammoniation on fiber fraction and digestibility of maize stover in vitro. Maize stover was treated by a multistage technique of different urea connect ration (8%, 4%, and 2%) and incubation for 14 days at room temperature.Digestibility of NDF, ADF, ADL, hemicellulose, and cellulose, in vitro dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N, total volatile fatty acid (VFA) and pH concentration were observed. However treatment maize stover and multistage ammoniation maize stover were composed by using T-test student as the statistic. There were not significantly different on fiber fraction and digestibility of maize stover. However, multistage ammoniation decreased ADF fraction by 56.67% to45.39%. The DM digestibility of multistage ammoniation on fiber fraction and digestibility of maize stover also increased by 35.18 to 45.91. The total VFA and N-ammonia of multistage ammoniation on fiber fraction and digestibility of maize stover also higher than control.Keywords: Ammoniation, Digestibility, in vitro, Maize stover

scholarly journals KECERNAAN DAN FERMENTABILITAS SUBSTRAT KOMBINASI MINERAL– FUNGI DALAM RUMEN

2019 ◽  
Vol 7 (2) ◽  
pp. 217
Author(s):  
Khanza Syahira Dhia ◽  
Kurnia A. Kamil ◽  
Hidayat Tanuwira
Keyword(s):  
Organic Matter ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Total Volatile ◽  
Variance Test ◽  
Dry Matter Digestibility ◽  
Studentized Range ◽  
Range Test ◽  
Total Volatile Fatty Acid

This study aims to determine the digestibility and fermentability of mineral-fungal combinations. The benefit of this study was to obtain data from the digestibility and fermentability of mineral-fungi combinations. The study was carried out in vitro with a completely randomized factorial 2 × 4 design with 3 replications. The variables observed were dry matter digestibility, organic matter digestibility, total VFA production (total volatile fatty acid), and NH3. The data obtained were then analyzed using variance test (ANOVA), if there was an influence on the variables measured, it will be followed by the Tukey’s Studentized Range test. The results showed that the combination of minerals gave a significant effect (P <0.05) and there was an interaction between mineral-fungi (P <0.05) in the digestibility of organic matter and VFA. The highest result was combination mineral with S. cerevisiae and as individual was combination Cu-Sc and Se-Sc. Keywords: Digestibility, Fermentability, Fungi, Mineral, Rumen.

scholarly journals Pengaruh Imbangan Jerami Padi, Dedak Padi dan Onggok Terfermentasi terhadap Kecernaan dan Produk Fermentasi Rumen Secara In Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 59-63
Author(s):  
S.N.O. Suwandyastuti ◽  
Efka Aris Rimbawanto ◽  
Ning Iriyanti
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Rice Straw ◽  
Rice Bran ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Industrial Wastes ◽  
Optimal Ratio ◽  
Dry Matter Digestibility

The influence of the ratio of fermented rice straw, rice bran and cassava solid waste upon the In vitro digestibility and rumen fermentation productABSTRACT. Chemical and physical treatments have been attempted to improve the utilization of agro industrial wastes, but the result is not efficient and caused pollution. Besides, biological treatments using the microbes have been used to improve the nutritive value and utilization of agro industrial wastes. The current experiment was conducted to find out the optimal ratio of fermented rice straw, fermented rice bran and fermented tapioca waste. There were five kinds of ratio of fermented rice straw, fermented rice bran and fermented tapioca waste, namely: K1 (70% fermented rice straw + 15% fermented rice bran + fermented tapioca waste), K2 (60% fermented rice straw + 20% fermented rice bran + 20% fermented tapioca waste), K3 (50% fermented rice straw + 25% fermented rice bran + 25% fermented tapioca waste), K4 (40% fermented rice straw + 30% fermented rice bran + 30% fermented tapioca waste) and K5 (30% fermented rice straw + 35% fermented rice bran + 35% fermented tapioca waste) An in vitro technique, using completely Randomize Block Design was applied and each treatment was repeated four times. Variables measured were Dry matter and Organic matter digestibility and rumen fermentation products (volatile fatty acid and N-NH3 concentration). The dry matter digestibility of K1, K2, K3, k4 and K5 was 29.39, 31.27, 32.33, 33.71 and 34.82%, respectively. The organic matter digestibility of K1, K2, K3, K4 and 5 was 30.82, 31.27, 32.73, 34.94, and 34.92, respectively. Volatile fatty acid concentrations of K1, K2, K3, K4 and K5 were 95.19, 91.77, 87.21, 104.31, 106.59 mM/l, respectively. N-NH3 concentrations of K1, K2, K3, K4 and K5 were 0.97, 0.93, 0.93, 1.00, 1.04 mM/l, respectively. Significant difference (P0.01) was only found in dry matter digestibility among treatments, while others variables were not significantly different among treatments. It was indicated that the optimal ratio was K4.

The effect of including Colombian rice polishings in the diet on rumen fermentation in vitro

1992 ◽  
Vol 54 (2) ◽  
pp. 275-280 ◽  
Author(s):  
D. Cardenas Garcia ◽  
C. J. Newbold ◽  
H. Galbraith ◽  
J. H. Topps
Keyword(s):  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
Total Volatile ◽  
Cellulolytic Bacteria ◽  
Medium Quality ◽  
Total Volatile Fatty Acid

AbstractThe effect of including full-fat Colombian rice polishings, at 250 or 500 g/kg dry matter (DM) or defatted Colombian rice polishings (at 500 g/kg DM) on the fermentation of a basal diet of dried grass and medium-quality hay, in the rumen simulation technique (Rusitec), was investigated. With diets which contained 0, 0·25, 0·50 full-fat or 0·50 defatted, proportions of rice polishing, values for pH were depressed (7·22, 7·19, 7·11, 7·06 (s.e.d. 0·05)) and total volatile fatty acid concentrations were increased (52·8, 52·5, 75·5, 754 (s.e.d. 2·1) mmol/l) at the high levels of inclusion of rice polishings. Concentrations of ammonia (130, 140, 228, 209 (s.e, d. 64) mg/l) and total bacterial numbers (2·32, 2·70, 3·55, 442 (s.e.d. 0·82) × 108 per ml) were elevated by rice polishings inclusion. Numbers of cellulolytic bacteria (2·52, 1·74, 1·84, 2·10 (s.e.d. 0·60) × 106 per ml) and protozoa (19·8, 16·2, 15·8, 22·2 (s.e.d. 1·51) × 103 per ml were depressed (the latter significantly P < 0·01) by the inclusion offull-fat, but not by defatted, rice polishings.

scholarly journals USING SAGO PITH AS ADHESIVE SUBSTANCE IN VITRO DIGESTIBILITY OF COMPLETE RATION BISCUIT

2020 ◽  
Vol 4 (1) ◽  
pp. 91-101
Author(s):  
Shirley Fredriksz ◽  
Lily Joris
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Rice Bran ◽  
Volatile Fatty Acid ◽  
In Vitro Digestibility ◽  
Elephant Grass ◽  
Total Volatile ◽  
Rumen Ph ◽  
Total Volatile Fatty Acid

The objective of the study was to determine in-vitro digestibility of complete ration biscuit using sago pith as adhesive substance. The experiment was arranged in Complete Randomized Design with 5 treatments and 5 replications. The treatments were P1 (50% mini elephant grass+ 20%  leucaena + 30% sago pith + 0% rice bran), P2 (50% mini elephant grass+ 20%  leucaena + 25% sago pith + 5% rice bran), P3 (50% mini elephant grass+ 20%  leucaena + 20% sago pith +10% rice bran ) and P4 (50% mini elephant grass+ 20%  leucaena + 15% sago pith + 15% rice bran). Variables measured were digestibility of dry (DM) and organic matter (OM), NH3 level, total volatile fatty acid (VFA), and rumen pH. The results show that the treatment has no significant effect on vitro digestibility of dry and organic matter. In vitro -digestibility is ranging from   90.7 – 91.4% and 87.5 – 88.4 % for DM and OM, respectively. In-vitro test on complete ration biscuit which consists of sago pith, rice bran, mini elephant grass and leucaena leucocephala has no significant  (P>0.05) on NH3 level, total volatile fatty acid and rumen pH. In conclusion, sago pith can be potentially used as adhesive substance in formulating complete ration biscuit.  

The effects of essential oil and condensed tannin on fermentation and methane production under in vitro conditions

2016 ◽  
Vol 56 (10) ◽  
pp. 1707 ◽  
Author(s):  
Brittany Pinski ◽  
Mevlüt Günal ◽  
Amer A. AbuGhazaleh
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Condensed Tannin ◽  
Total Volatile ◽  
Tannin Extract ◽  
Ch4 Production ◽  
N Concentration ◽  
Total Volatile Fatty Acid

The potential of five different essential oils (EO) and quebracho condensed tannin extract (QCT) as antimethanogenic additives in ruminant feeds were investigated. The first experiment was conducted to screen the effects rosemary oil, sage oil, cinnamon oil (CNO), eucalyptus oil and myrrh oil at 500 mg/L of culture fluid on methane (CH4) production under in vitro conditions. Rumen contents were collected from a cannulated Holstein dairy cow and used for a 24-h batch-culture experiment. Treatments were a control (CON) or CON plus EO at 500 mg/L. Results showed that CNO decreased CH4 production and, therefore, was selected for Experiment 2. The second experiment was designed to test the effects of CNO at three different dose levels on CH4 production and fermentation in 24-h batch-culture experiments. Treatments were CON or CON plus CNO supplemented at 125, 250 and 500 mg/L. Relative to CON, CNO decreased total gas production at the 250 and 500 mg/L doses. All doses of CNO decreased CH4 production. Total volatile fatty acid production was lower in cultures incubated with CNO at the 500 mg/L. Ammonia-N concentration decreased in cultures incubated with CNO at the 500 mg/L. The third experiment was designed to test the effects of QCT on CH4 production and fermentation in 24-h batch cultures. Treatments were CON or CON plus QCT at 25, 50 and 75 g/kg of diet DM. Relative to CON, total volatile fatty acid concentration increased with the 50 g/kg QCT, but was similar to the 25 and 75 g/kg QCT. The proportions of acetate decreased, while the proportions of propionate increased with the 25 g/kg QCT compared with CON. Methane production was not affected in cultures incubated with QCT. Relative to CON, all doses of QCT decreased ammonia-N concentration. In conclusion, results from the present study showed that except for CNO, EO tested in the study had no effects on rumen CH4 production. Addition of CNO to rumen cultures at 125 and 250 mg/L reduced CH4 production without negative effects on rumen fermentation. Quebracho condensed tannin-extract supplementation had no effects on CH4 production and fermentation parameters except for ammonia-N concentration.

Effect of monensin on in vitro fermentation of maize starch by hindgut contents of cattle

1990 ◽  
Vol 115 (3) ◽  
pp. 389-392 ◽  
Author(s):  
M. Marounek ◽  
O. Petr ◽  
L. Machañová
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Maize Starch ◽  
Total Volatile ◽  
Molar Percentage ◽  
In Vitro Fermentation ◽  
Consistent Effect ◽  
Total Volatile Fatty Acid

SUMMARYContents of the caecum and the colon of four steers and four cows fed concentrate-plus-roughage diets were obtained at an abattoir, transferred to the laboratory and incubated anaerobically with maize starch in the presence (10mg/l) or absence of monensin. Samples taken at the end of incubation were assayed for fermentation acids and methane production.Monensin significantly increased the molar percentage of propionate and decreased that of butyrate. Acetate percentage was not significantly influence by the addition of monensin. There was no consistent effect of monensin on total volatile fatty acid concentrations. Monensin decreased methanogenesis in all incubations. The production of methane was small, only 1 mmol/13–50 mmol of volatile fatty acid.

scholarly journals Effect Of Dietary Concentrate And Bypass Fat Supplements On In Vitro Rumen Fermentation, Digestibility And Rumen Microbial Population In Buffaloes

2020 ◽  
Author(s):  
AMIRUL FAIZ MOHD AZMI ◽  
A. Hafandi ◽  
Y. M. Goh ◽  
MOHD ZAMRI SAAD ◽  
A. B. Md ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Microbial Population ◽  
Nutritional Composition ◽  
Gas Production ◽  
Total Volatile ◽  
Brachiaria Decumbens ◽  
Total Volatile Fatty Acid ◽  
Bypass Fat

Abstract Background: The effect of Brachiaria decumbens supplemented with concentrate and bypass fat ratio 100, 70:30, 70:15:15 (w/w) on nutritional composition, in vitro rumen fermentation and microbial population in Murrah cross and Swamp buffaloes were investigated. Incubation were conducted using rumen fluid obtained from the breed of each buffalo which were fed the same based diet (100% Brachiaria decumbens). For the in vitro fermentation characteristic after 48h incubation, total gas production, pH, total volatile fatty acid (TVFA), apparent rumen degradable carbohydrate (ARDC), methane (CH4) and ammonia (NH 3) were determined. The molecular technique also was used to quantify rumen total bacteria, total protozoa, total methanogens, Fibrobacter succinogens and Ruminococcus albus.Results: The results revealed Diet C showed significantly highest in dry matter, crude fat, metabolized energy and optimum in crude protein and carbohydrate value while lower in crude fiber as compared to Diet B and Diet A (P<0.05). In both breed were showed parameters of gas production, total volatile fatty acid (TVFA) and its proportion, and total microbial population were increased parallelly with the increase of 30% concentrate levels in Diet B, while Diet C was moderate with the presence of 4% bypass fat and 26% of concentrate (P<0.05). The methane concentration as well the total methanogens population increased significantly (P<0.05) in Diet B when compared among other diets, but no significant difference was found when comparing between buffalo species. Meanwhile, pH value was slightly decreased with the dietary supplementation in both breeds, but the population of cellulolytic bacteria was not affected.Conclusions: This study showed that dietary concentrate and bypass fat supplementation had improved nutritional composition, in vitro fermentation characteristics by increasing VFA concentration, altering total microbial population, and potentially used as new diet for buffaloes in Malaysia.

Effect of 9,10-Anthraquinone on rumen methane production as studied in vitro and in vivo

2003 ◽  
Vol 2003 ◽  
pp. 122-122
Author(s):  
V. Fievez ◽  
B. Vlaeminck ◽  
W. Steinberg ◽  
I. Immig ◽  
D. Demeyer
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Rumen Fermentation ◽  
Dose Effect ◽  
Total Volatile ◽  
Total Volatile Fatty Acid

In vitro supplementation of 0.05% [on a substrate basis (wt/wt)] - but not of 0.01% - of 9,10-Anthraquinone (AQ) inhibited rumen methanogenesis, reduced total volatile fatty acid (VFA) concentrations and molar proportions of acetate (Acet), increased proportions of propionate (Prop) and butyrate (But) and resulted sometimes in H2 accumulation (Garcia-Lopez et al., 1996). In vivo administration of high amounts of AQ [5% on a substrate basis (wt/wt)] to lambs depressed CH4 and increased H2 concentrations in ruminal gases during the complete 19 days of administration, whereas original concentrations were re-installed within 6 days after the removal of AQ from the diet (Kung et al., 1996). In this experiment we aimed to study the dose effect of AQ on in vitro rumen fermentation and modifications to rumen fermentation when administering 0.05% of AQ in vivo.

Sign in / Sign up

Export Citation Format

Share Document