scholarly journals Sonoelectrochemical Synthesis of Silver Nanoparticles in Sodium Polyacrylate Solution

2021 ◽  
Vol 11 (4) ◽  
pp. 12202-12214
Keyword(s):  
Silver Nanoparticles ◽  
Nanoparticle Synthesis ◽  
Ultrasonic Field ◽  
Sodium Polyacrylate ◽  
Silver Colloids ◽  
High Antibacterial Activity ◽  
Vis Spectroscopy ◽  
Sonoelectrochemical Synthesis ◽  
The Stability ◽  
Increasing Temperature

The paper shows the effectiveness of a “green” synthesis of silver nanoparticles (AgNPs) in sodium polyacrylate (NaPA) solutions by sonoelectrochemical method using silver sacrificial anodes. Using the cyclic voltammetry in the ultrasonic field in the range of E from 1.0 to -1.0 V, the temperature and NaPA concentration are the main parameters influencing the rate of synthesis and the size of AgNPs. As these parameters increase, the rate of nanoparticle synthesis increases. According to TEM studies, with increasing temperature and decreasing NaPA concentration, there is a tendency to increase the size of AgNPs. However, in all of the cases, the size of AgNPs does not exceed 30 nm. Using the UV–Vis spectroscopy, it was found that the position of the absorption peak at c.a. 500 nm, corresponding to the silver nanoparticles, is practically not shifted during numerous cycles. This fact may indicate the stability of sonoelectrochemical synthesis of AgNPs in time. Synthesized AgNPs revealed high antibacterial activity against gram-positive and gram-negative strains of typical pathogens of nosocomial infections, demonstrating the prospect of using sonoelectrochemical technique for obtaining silver colloids as a component of bactericidal drugs.

scholarly journals Sonoelectrochemical synthesis of silver nanoparticles in polyvinylpyrrolidone solutions

2021 ◽  
Vol 4 (1) ◽  
pp. 82-87
Author(s):  
M. V. Shepida ◽  
◽  
M. A. Sozanskyi ◽  
Yu. V. Sukhatskiy ◽  
A. S. Mazur ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Formation Rate ◽  
Ultrasonic Field ◽  
Colloidal Solutions ◽  
Complex Ions ◽  
Diffusion Kinetic ◽  
Wide Range ◽  
Sonoelectrochemical Synthesis ◽  
The Stability ◽  
Sonochemical Reduction

The results of investigations of the influence of main parameters (surfactant concentration and temperature) on the synthesis of silver nanoparticles (AgNPs) by the sonoelectrochemical method in polyvinylpyrrolidone (PVP) solutions by cyclic voltammetry (CVA) are presented. It is shown that the ultrasonic field (22 kHz) leads to an increase in the anodic and cathodic currents by ~30 %. A scheme of the AgNPs formation has been proposed, which includes the following main processes: 1) dissolution of sacrificial silver anodes at E = 0.2...1.0 V with the formation of [AgPVP]+ complex ions; 2) cathodic and sonochemical reduction of the latter to Ag(0); 3) formation of AgNPs. It has been established that with an increase in PVP concentration from 1 to 4 g·L-1, the anodic and cathodic currents decrease by 40–60 %. The formation rate of AgNPs also decreases. The growth of anodic and cathodic currents and the formation rate of nanoparticles in the range of 20…60 °C corresponds to the diffusion-kinetic action of the temperature factor. The CVA curves practically do not change in time, which indicates the stability of anodic and cathodic processes at prolonged sonoelectrochemical synthesis. The character of the UV-Vis spectra of AgNPs colloidal solutions in PVP with the 405…410 nm absorption maximum is the same in a wide range of nanoparticle concentrations.

scholarly journals Sunlight-Induced Synthesis of Non-Target Biosafety Silver Nanoparticles for the Control of Rice Bacterial Diseases

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 2007
Author(s):  
Hongyi Shang ◽  
Zehao Zhou ◽  
Xuemin Wu ◽  
Xuefeng Li ◽  
Yong Xu
Keyword(s):  
Silver Nanoparticles ◽  
Acute Toxicity ◽  
Laser Scanning ◽  
Control Plant ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Bacterial Diseases ◽  
Rice Bacterial Blight ◽  
High Antibacterial Activity ◽  
Vis Spectroscopy

Silver is an important and efficient bactericide. Nanoscale silver has a large specific surface area, high target adhesion, strong permeability and high bactericidal activity. At present, the control of plant bacterial diseases is difficult, and the resistance of plant bacterial pathogens develops rapidly. Silver nanoparticles are expected to become a new generation of agrochemical to control plant bacterial diseases. In this study, a simple and green natural sunlight-induced method was used to prepare carboxymethylcellulose sodium-stabilized silver nanoparticles (CMC-SNs) with a particle size of around 13.53 ± 4.72 nm. CMC-SNs were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), energy-dispersive spectrometry (EDS), X-ray diffraction (XRD) and UV-vis spectroscopy and found to be spherical and evenly dispersed. The bacteriostatic activity of the CMC-SNs toward Xanthomonas oryzae pv. oryzae (Xoo) was tested. The minimum inhibitory concentration (MIC) of CMC-SNs to Xoo was 1 mg/L, and the minimum bactericidal concentration (MBC) was 2 mg/L. In addition, the antibacterial mechanism was studied by scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM), which confirmed that the CMC-SNs had high antibacterial activity. In order to verify its impact on the environment, we conducted an acute toxicity test on zebrafish and found that Half lethal concentration (LC50) > 100 mg/L in zebrafish, or no acute toxicity. The ability of CMC-SNs to control rice bacterial blight was verified by a pot experiment.

scholarly journals Green and Rapid Synthesis of Anticancerous Silver Nanoparticles bySaccharomyces boulardiiand Insight into Mechanism of Nanoparticle Synthesis

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Abhishek Kaler ◽  
Sanyog Jain ◽  
Uttam Chand Banerjee
Keyword(s):  
Silver Nanoparticles ◽  
Reaction Time ◽  
Cell Mass ◽  
Nanoparticle Synthesis ◽  
Silver Ions ◽  
Saccharomyces Boulardii ◽  
Breast Cancer Cell Lines ◽  
X Rays ◽  
Metallic Nanoparticle ◽  
Vis Spectroscopy

Rapidly developing field of nanobiotechnology dealing with metallic nanoparticle (MNP) synthesis is primarily lacking control over size, shape, dispersity, yield, and reaction time. Present work describes an ecofriendly method for the synthesis of silver nanoparticles (AgNPs) by cell free extract (CFE) ofSaccharomyces boulardii. Parameters such as culture age (stationary phase growth), cell mass concentration (400 mg/mL), temperature (35°C), and reaction time (4 h), have been optimized to exercise a control over the yield of nanoparticles and their properties. Nanoparticle (NP) formation was confirmed by UV-Vis spectroscopy, elemental composition by EDX (energy dispersive X-rays) analysis, and size and shape by transmission electron microscopy. Synthesized nanoparticles had the size range of 3–10 nm with high negative zeta potential (−31 mV) indicating excellent stability. Role of proteins/peptides in NP formation and their stability were also elucidated. Finally, anticancer activity of silver nanoparticles as compared to silver ions was determined on breast cancer cell lines.

scholarly journals Synthesis of Silver Nanoparticles using Egg White: Dye Degradation and Antimicrobial Potential

2021 ◽  
Vol 12 (2) ◽  
pp. 2361-2372
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Dye Degradation ◽  
Egg White ◽  
Blue Dye ◽  
Second Phase ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
The Stability ◽  
And Staphylococcus Aureus

In recent years, developing nanoparticles with green processes is gaining huge attention due to its cost-effectiveness, simplicity and non–toxic precursors. The present study utilized the potential of egg white for the synthesis of stable silver nanoparticles (EW-AgNPs). In order to characterize the EW-AgNPs, various techniques have been employed. UV-vis spectroscopy (300-700nm) was used to study the λmax, which highlighted the peak at 422nm. Further, the stability of synthesized EW-AgNPs was studied using Zeta potential, the value of -16.4 mV was obtained, indicating the stability of developed EW-AgNPs in the solution. Transmission electron microscopy was used specifically to visualize the shape and size of synthesized EW-AgNPs, the images showed spherical to the diverse shape of EW-AgNPs. In the first phase, the EW-AgNPs were studied for dye degradation along with NaBH4. The enhanced dye degradation of blue dye was obtained with EW-AgNPs+NaBH4, showing 90- 100% degradation from 100- 25 mgL-1 dye solution, respectively. Further, in the second phase, antimicrobial activity (Zone of Inhibition) of EW-AgNPs was analyzed against Escherichia coli and Staphylococcus aureus. A higher ZOI was obtained for E.coli (16mm) than S. aureus (12.4mm). The present study proved egg white's ability to develop stable silver nanoparticles, which was further found to be effective for blue dye degradation and antimicrobial activity.

scholarly journals Rapid and Efficient Synthesis of Silver Nanofluid Using Electrical Discharge Machining

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Kuo-Hsiung Tseng ◽  
Heng-Lin Lee ◽  
Chih-Yu Liao ◽  
Kuan-Chih Chen ◽  
Hong-Shiou Lin
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Process Parameters ◽  
Electrical Discharge ◽  
Electrical Discharge Machining ◽  
Silver Ions ◽  
Nano Fluid ◽  
Vis Spectroscopy ◽  
The Stability ◽  
The Relationship

The electrical discharge machining (EDM) system has been proven feasible as a rapid and efficient method for silver nanofluid preparation. This study prepared the silver nano-fluid via EDM and investigated the relationship between its process parameters and product characteristics. The prior study had found that the silver nano-fluid prepared by EDM contained both silver nanoparticles and silver ions. Silver ions had revealed the cause of the high suspension of the silver nanoparticles. To examine the relationship between the stability of silver nanofluid and the process parameters, this study quantified the relationship of process parameters to the material removal rate (MRR) of silver electrode and silver ion output rate (IOR) in the fluid, in order to achieve the most effective process parameter condition. Furthermore, the stability of silver nano-fluid was analyzed by various devices, including UV-Vis spectroscopy, size-distribution, and Zeta-potential analyzer. The effects of MRR, IOR, particle size, Zeta-potential, and optical properties of silver nanofluid under different process parameters are also discussed.

scholarly journals Fortunella japonica extract as a reducing agent for green synthesis of silver nanoparticles

2018 ◽  
Vol 7 (3) ◽  
pp. 1570
Author(s):  
Nguyen Phung Anh ◽  
Truong Thi Ai Mi ◽  
Duong Huynh Thanh Linh ◽  
Nguyen Thi Thuy Van ◽  
Hoang Tien Cuong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Volume Ratio ◽  
Diffusion Method ◽  
High Antibacterial Activity ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Average Size

A rapid way of synthesizing silver nanoparticles (AgNPs) by treating Ag+ ions with a green Fortunella Japonica (F.J.) extract as a combined reducing and stabilizing agent was investigated. The reaction solutions were monitored using UV-Vis spectroscopy, the size and shape of crystals were determined by scanning electron microscopy and transmission electron microscopy, the crystalline phases of AgNPs were presented by X–ray diffraction, and the relation of nanoparticles with Fortunella Japonica extract was confirmed using fourier transform infrared spectroscopy. The results indicated that no formation of AgNPs had taken place in the dark during 24 hours at room temperature and 40 oC. Meanwhile, it was found that the rate of AgNPs formation increased rapidly under the sunlight. The effects of the synthesis factors on the AgNPs formation were investigated. The suitable conditions for the synthesis of AgNPs using F.J. extract were determined as follows: F.J. extract was mixed with AgNO3 1.75 mM solution with the volume ratio of 3.5 AgNO3 solution/1.5 F.J. Extract, stirred 300 rpm for 150 minutes at 40 oC under sunlight illumination. At these conditions, AgNPs showed high crystalline structure with the average size of 15.9 nm. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E. coli and B. subtilis bacteria. The green synthesized AgNPs performed high antibacterial activity against both bacteria.  

scholarly journals Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 2082 ◽  
Author(s):  
Salem S. Salem ◽  
Ehab F. EL-Belely ◽  
Gniewko Niedbała ◽  
Maryam M. Alnoman ◽  
Saad El-Din Hassan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Inhibition Zone ◽  
In Vitro Cytotoxicity ◽  
Ag Nps ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
The Stability

An endophytic strain of Streptomyces antimycoticus L-1 was isolated from healthy medicinal plant leaves of Mentha longifolia L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV–vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs with a particle size of 13–40 nm. Further, the stability of the Ag-NPs was assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month or by measuring the NPs surface charge (−19.2 mV) by zeta potential analysis (ζ). The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations (6.25–100 ppm) against the pathogens Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium with a clear inhibition zone ranging from (9.5 ± 0.4) nm to (21.7 ± 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC50) = 5.7 ± 0.2 ppm). With respect to antibacterial and in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected as a safe dose for loading onto cotton fabrics. The scanning electron microscopy connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric elements. Moreover, the nano-finished fabrics exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing cycles, indicating the stability of the treated fabrics.

scholarly journals Biological Synthesis of Silver Nanoparticles by Cell-Free Extract ofSpirulina platensis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Gaurav Sharma ◽  
Nakuleshwar Dut Jasuja ◽  
Manoj Kumar ◽  
Mohammad Irfan Ali
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Large Scale ◽  
Cost Effective ◽  
Biological Synthesis ◽  
Cell Free Extract ◽  
Tem Analysis ◽  
High Antibacterial Activity ◽  
Vis Spectroscopy ◽  
Average Size

The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract ofSpirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract ofS. platensisshowed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is,Escherichia coli, MTCC-9721;Proteus vulgaris, MTCC-7299;Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is,Staphylococcus aureus, MTCC-9542;S. epidermidis, MTCC-2639;Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is31.3±1.11inP. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.

scholarly journals ANTIMICROBIAL AND ANTICANCER ACTIVITY OF SILVER NANOPARTICLES FROM EDIBLE MUSHROOM: A REVIEW

2017 ◽  
Vol 10 (3) ◽  
pp. 37 ◽  
Author(s):  
Priyadarshni Karuppiah Chandran ◽  
Mahalingam Pambayan Ulagan
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Low Cost ◽  
Nanoparticle Synthesis ◽  
Colon Adenocarcinoma ◽  
Breast Adenocarcinoma ◽  
Metallic Silver ◽  
Liver Carcinoma ◽  
X Ray ◽  
Vis Spectroscopy

ABSTRACTBiologically inspired nanoparticle synthesis is currently a rapid expanding area of research in nanotechnology. Nanoparticle synthesis utilizing thebioresources such as plants and microbes appears to be a viable, low-cost, and eco-friendly approach. Especially mushrooms can be used for largescalesynthesis of silver nanoparticles as mushroom produces many proteins that reduce the silver nitrate during the biosynthesis. Silver nanoparticlescan be characterized using ultraviolet-visible (UV-VIS) spectroscopy, fourier transform infrared spectroscopy, X-ray diffraction, scanning electronmicroscopy, energy dispersive X-ray, and transmission electron microscope. Silver nanoparticles possess high antibacterial activity since silver indifferent forms has been extensively used as a medicine for curing diseases and promote wound healing. Silver nanoparticles have high surfacespecific area, which will lead to excellent antimicrobial activity as compared with bulk metallic silver. Further, the silver nanoparticles show anticanceractivity against various cell lines such as human epidermoid larynx carcinoma (HEP-2), colon adenocarcinoma (HCT-116), breast adenocarcinoma(MCF-7), liver carcinoma (Hep-G2), and intestinal adenocarcinoma (Caco2) were well documented. This review intends to present green synthesis ofsilver nanoparticles and their application as antimicrobial and anticancer agents.Keywords: Silver nanoparticles, Bioresources, Mushroom, Antimicrobial activity, Anticancer property.

scholarly journals Investigation of Nanoparticle Metallic Core Antibacterial Activity: Gold and Silver Nanoparticles against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 22 (4) ◽  
pp. 1905
Author(s):  
Jimmy Gouyau ◽  
Raphaël E. Duval ◽  
Ariane Boudier ◽  
Emmanuel Lamouroux
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metallic Nanoparticles ◽  
Catalytic Reduction ◽  
Gold And Silver Nanoparticles ◽  
High Antibacterial Activity ◽  
Vis Spectroscopy ◽  
Broth Microdilution Method

Multidrug-resistant (MDR) bacteria constitute a global health issue. Over the past ten years, interest in nanoparticles, particularly metallic ones, has grown as potential antibacterial candidates. However, as there is no consensus about the procedure to characterize the metallic nanoparticles (MNPs; i.e., metallic aggregates) and evaluate their antibacterial activity, it is impossible to conclude about their real effectiveness as a new antibacterial agent. To give part of the answer to this question, 12 nm gold and silver nanoparticles have been prepared by a chemical approach. After their characterization by transmission electronic microscopy (TEM), Dynamic Light Scattering (DLS), and UltraViolet-visible (UV-vis) spectroscopy, their surface accessibility was tested through the catalytic reduction of the 4-nitrophenol, and their stability in bacterial culture medium was studied. Finally, the antibacterial activities of 12 nm gold and silver nanoparticles facing Staphylococcus aureus and Escherichia coli have been evaluated using the broth microdilution method. The results show that gold nanoparticles have a weak antibacterial activity (i.e., slight inhibition of bacterial growth) against the two bacteria tested. In contrast, silver nanoparticles have no activity on S. aureus but demonstrate a high antibacterial activity against Escherichia coli, with a minimum inhibitory concentration of 128 µmol/L. This high antibacterial activity is also maintained against two MDR-E. coli strains.

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