scholarly journals Anti-diabetic Activity of Buchanania lanceolata Wight Extracts in Streptozocin persuade Diabetic Animal Model

2021 ◽  
Vol 12 (5) ◽  
pp. 6741-6752
Keyword(s):  
Glucose Uptake ◽  
Aqueous Extract ◽  
Lipid Level ◽  
Folk Medicine ◽  
Diabetic Animal ◽  
Albino Rats ◽  
Diabetic Patients ◽  
Oral Toxicity ◽  
Myoblast Cell

Diabetes mellitus is a well-established metabolic syndrome that originates from a complete or virtual requirement of insulin and low insulin action, which leads to hyperglycemia and malformation in the regulation of lipids, proteins, and carbohydrates. Micro and macrovascular difficulties are mainly developed in diabetic patients when compared with normal individuals. The plant variety selected for this study was Buchanania lanceolata Wight (B. lanceolata), which comes under the family of Anacardiaceae, which was originated in India and Myanmar. In the current research, an aqueous extract of B. lanceolata was isolated from the plant barks. Glucose uptake was carried out by using L6 myoblast cell lines, and the results showed an improved glucose uptake in vitro by the aqueous extract of bark of B. lanceolata. Moreover, it was also studied for acute dose oral toxicity and anti-diabetic studies in Wistar albino rats. The aqueous extract did not demonstrate any toxic conditions or mortality at single dose feeding of 2000 mg/kg/p.o and was examined for 15 days. It was also found out the variations in glucose level, hematology parameters, lipid level, and biochemical factors of both control and treated animals. Currently, the natural drug gained attention among researchers because of the problems developed by the allopathic remedial representative. The examination of anti-diabetic agents of an herbal source that are used in folk medicine is thus of huge significance. Management of diabetes with artificial medicines is an expensive and high probability for side effects. As a result, it is necessary to develop plant-based medicines for diabetes. B. lanceolata is a promising herbal drug for diabetic treatment not only in Ayurveda but also in other folk medicine.

scholarly journals Evaluation of Aqueous Extract of Alphonsea sclerocarpa for in vivo and in vitro Antidiabetic Potentials in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ravi Shankar N ◽  
Ram Kishore ◽  
Puranik SB
Keyword(s):  
Blood Glucose ◽  
Glucose Uptake ◽  
Aqueous Extract ◽  
Insulin Level ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Oral Glucose ◽  
In Vivo Evaluation

The purpose of current investigation was to investigate in vivo and in vitro anti-diabetic potentials of aqueous extract of Alphonsea sclerocarpa leaves against alloxan induced diabetes in albino rats. Two in vivo and one in vitro methods were performed for the evaluation of aqueous extract for antidiabetic activity. For in-vivo evaluation, diabetes was induced in albino rats by administering a single dose of alloxan. The study was designed to test the acute effect of aqueous extract of Alphonsea sclerocarpa (AEAS) to reduce blood glucose in OGTT. The chronic study of 21 days was performed against diabetic rats and blood glucose was determined at 1st , 7 th, 14th and 21st day. In chronic in vivo study, serum parameters insulin, urea, creatinine, total cholesterol, triglycerides, ALT and AST were also estimated at 21st day to determine the effects of aqueous and aqueous extracts on complications of diabetes mellitus. Glucose uptake by hemidiaphragm assay was performed to test the ability of extract to utilize glucose. In Oral Glucose Tolerance Test, standard glibenclamide and aqueous extract (200mg/kg and 400mg/kg) treated animals have shown significant reduction in blood glucose at 90 mins but at 120 mins. In chronic model the aqueous extract effectively reduced blood glucose levels (P<0.001) at 14th and 21st day of study in therapeutic groups and effect was comparable to that of standard. The extract could also significantly (P<0.001) reduce concentrations of SGOT, triglycerides, cholesterol and urea in serum and significantly (P<0.001) increased the insulin level in blood which proves beneficial effects of the extract in diabetes. The change in concentrations of SGPT and urea were less significant (P>0.01). The presence of extract in glucose uptake assay could significantly increase utilization of the glucose by rat hemidiaphragm. The aqueous extract of Alphonsea sclerocarpa possess significant antidiabetic properties against alloxan induced diabetic animals.

scholarly journals Delving into the Antiurolithiatic Potential of Tribulus terrestris Extract Through –In Vivo Efficacy and Preclinical Safety Investigations in Wistar Rats

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jyoti Kaushik ◽  
Simran Tandon ◽  
Rishi Bhardwaj ◽  
Tanzeer Kaur ◽  
Surinder Kumar Singla ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Kidney Stones ◽  
Wistar Rats ◽  
Lethal Dose ◽  
Tribulus Terrestris ◽  
Oral Toxicity ◽  
Preclinical Safety

Abstract Modern treatment interventions for kidney stones are wrought with side-effects, hence the need for alternative therapies such as plant-based medicines. We have previously documented through in vitro studies that statistically optimized aqueous extract of Tribulus terrestris (Zygophyllaceae family) possesses antiurolithic and antioxidant potential. This provides strong scientific foundation to conduct in vivo efficacy and preclinical safety studies to corroborate and lend further proof to its ability to prevent and cure kidney stones. The preventive and curative urolithiatic efficacy in experimentally induced nephrolithiatic Wistar rats, along with preclinical toxicity was evaluated following oral administration of statistically optimized aqueous extract of T. terrestris. Treatment showed augmented renal function, restoration of normal renal architecture and increase in body weight. Microscopic analysis of urine revealed excretion of small sized urinary crystals, demonstrating that treatment potentially modulated the morphology of renal stones. Tissue enzymatic estimation affirmed the antioxidant efficacy of treatment with reduced free radical generation. Significant upregulation of p38MAPK at both the gene and protein level was noted in hyperoxaluric group and interestingly treatment reversed it. Acute oral toxicity study established the Median Lethal Dose (LD50) to be greater than 2000 mg/kg body weight (b.wt.) No observed adverse effect level (NOAEL) by repeated oral toxicity for 28 days at 750 mg/kg b.wt. was noted. This study lends scientific evidence to the safe, preventive and curative potential of statistically optimized aqueous extract of T. terrestris at a dose of 750 mg/kg b.wt. and suggests that the extract shows promise as a therapeutic antiurolithic agent.

scholarly journals Glycemia Lowering Effect of an Aqueous Extract of Hedychium coronarium Leaves in Diabetic Rodent Models

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 629 ◽  
Author(s):  
Ling-Shan Tse ◽  
Po-Lin Liao ◽  
Chi-Hao Tsai ◽  
Ching-Hao Li ◽  
Jiunn-Wang Liao ◽  
...  
Keyword(s):  
Animal Models ◽  
Aqueous Extract ◽  
Low Density Lipoprotein ◽  
Folk Medicine ◽  
Density Lipoprotein ◽  
Insulin Level ◽  
Diabetic Animal ◽  
Hedychium Coronarium ◽  
History Of ◽  
History Of Use

Hedychium coronarium has a long history of use worldwide as a food and in folk medicine. In this study, we aimed to investigate the effect of an aqueous extract of H. coronarium leaves (HC) on type 2 diabetes mellitus (T2DM). Two types of animal models were used in this study: Streptozotocin (STZ)-induced T2DM (Wistar rats; N = 8) and C57BKSdb/db mice (N = 5). After treatment with HC for 28 days, glucose tolerance improved in both of the diabetic animal models. As significant effects were shown after 14 days of treatment in the STZ-induced T2DM model, we carried out the experiments with it. After 28 days of treatment with HC, the levels of cholesterol, triglyceride, high-density lipoprotein, and low-density lipoprotein were significantly improved in the STZ-induced T2DM model. The lesions degree of islet β-cells was decreased after the HC treatment. Although the insulin level increased moderately, the aldosterone level was significantly decreased in the HC-treated groups, suggesting that aldosterone might play an important role in this effect. In summary, HC is a natural product and it is worth exploring its effect on T2DM.

‘In vivo’ and ‘in vitro’ activity of Larrea divaricata Cav. on EL-4 cells

2010 ◽  
Vol 30 (8) ◽  
pp. 965-971 ◽  
Author(s):  
Roberto Davicino ◽  
Rosario Alonso ◽  
Claudia Anesini
Keyword(s):  
Cell Proliferation ◽  
Aqueous Extract ◽  
Tritiated Thymidine ◽  
Folk Medicine ◽  
Thymidine Uptake ◽  
Stimulatory Action ◽  
T Lymphoma ◽  
Larrea Divaricata

Larrea divaricata is a plant widely used in folk medicine in Argentina. It has been demonstrated that an aqueous extract of L. divaricata possesses a biphasic effect on cell proliferation, at low concentrations exerts a stimulatory action and at high concentrations exerts anti-proliferative effects upon the T lymphoma BW 5147; therefore, we propose in this paper to test the effect of the extract ‘in vitro’ and ‘in vivo’ in another T-cell lymphoma named EL-4. It was analyzed ‘in vitro’ cell proliferation by tritiated thymidine uptake and the effect of the extract on tumors induced in mice analyzing tumor progression and survival.The results showed that the aqueous extract induced the proliferation of tumor cells at all the concentrations studied. The results ‘in vivo’ showed that the aqueous extract stimulated significantly the size of tumors and that untreated mice lived longer than those treated. It is important to be very careful when plant extracts are selected for the treatment of several diseases. Consequently, before using a plant extract, specific scientific studies must be undertaken on different models to certificate therapeutic and adverse effects. Moreover, it can be said that L. divaricata has a specific anti-tumor mechanism of action depending on the targets.

scholarly journals Investigation of ginger (Zingiber officinale) aqueous extract as an anti-diabetic in vitro

2021 ◽  
Vol 913 (1) ◽  
pp. 012108
Author(s):  
P Pakan ◽  
K Lidia ◽  
M Riwu
Keyword(s):  
Side Effects ◽  
Glucose Uptake ◽  
Aqueous Extract ◽  
Zingiber Officinale ◽  
Control Group ◽  
Plain Water ◽  
Ginger Extract ◽  
Mouse Tissues ◽  
Treatment Of Diabetes

Abstract Diabetes mellitus is a condition of metabolic imbalance, indicated by a high level of blood glucose (hyperglycemia) resulting from a reduction of insulin secretion, action, or both. People with diabetes suffer from a lack or deficiency of insulin or insulin resistance. The metabolic imbalances are often not satisfactorily corrected using conventional medicines and even cause some side effects, which can be detrimental. Research on herbal medicines for the treatment of diabetes is urged by the need to reduce unwanted side effects common with conventional medicines/treatments used in glucose regulation. This study aims to investigate the antidiabetic effect of Ginger (Zingiber officinale) aqueous extract in improving the glucose uptake in mouse tissues in vitro. This study is a true experimental research design with a posttest-only control group design. There were three groups of mice in this study: the control group, which were only given plain water; the second group of mice with 5% aqueous ginger extract and the last group were given 25% aqueous ginger extract. All groups were given treatment for four consecutive weeks, then dissected their cardiac muscle, skeletal muscle, pancreas, and liver tissues to analyze the glucose uptake. The result showed that both the ginger aqueous extract groups were able to increase the glucose uptake of the mice. In conclusion, this research has shown that aqueous ginger extract may have improved the glucose uptake in most tissues of the mice in the groups. Therefore, ginger could have great potential as an alternative way in the treatment of diabetes type 2.

scholarly journals L-cysteine Supplementation Increases Blood Levels of Hydrogen Sulfide and Nitrite, and Decreases Insulin Resistance and Vascular Inflammation in Zucker Diabetic Rats

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 405-405
Author(s):  
Sushil Jain
Keyword(s):  
Insulin Resistance ◽  
Hydrogen Sulfide ◽  
Animal Studies ◽  
Vascular Inflammation ◽  
Diabetic Animal ◽  
Diabetic Rats ◽  
Blood Levels ◽  
Diabetic Patients ◽  
Zdf Rats

Abstract Objectives Diabetic patients have lower blood levels of hydrogen sulfide (H2S). H2S has potent antioxidant, anti-inflammatory and anti-atherosclerotic effects in vitro and in animal studies. This study examined the hypothesis that supplementation with L-cysteine, an endogenous precursor of H2S increases blood levels of H2S and lowers insulin resistance and vascular inflammation biomarkers in type 2 diabetes using Zucker diabetic (ZDF) rats as a model. Methods Starting at age of 6 weeks, ZDF rats were supplemented orally, daily gavages for 8 weeks with saline-placebo (D, n = 8) or L-cysteine (LC, n = 12, 1 mg/kg BW) and fed a high calorie diet. 6 weeks age rats without any supplementation were considered baseline (BL) rats. Results Fasting blood levels of D rats showed lower H2S and elevated HGb, MCP-1 and insulin resistance when compared with baseline in which there was no onset of diabetes. LC supplementation significantly (P &lt; 0.05) increased blood levels of H2S (37%), and NO2 (30%) and lowered levels of GHb (9%), MCP-1 (31%), TNF (31%) and HOMA insulin resistance (25%) compared with levels seen in saline supplemented D. The blood levels of GHb and IR showed a significant correlation (P &lt; 0.05) with concentrations of H2S and nitrite in LC-supplemented ZDF rats. Conclusions This shows that L-cysteine supplementation can increase levels of H2S and NO2 in diabetic animal model, and needs to be validated as an adjuvant therapy for the reduction of vascular inflammation and insulin resistance in the diabetic patient population. Funding Sources This study was supported by the NCCIH.

scholarly journals Biological Evaluation ofPupalia lappaceafor Antidiabetic, Antiadipogenic, and Hypolipidemic Activity BothIn VitroandIn Vivo

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Vivek Kumar ◽  
Parag Jain ◽  
Kalpana Rathore ◽  
Zabeer Ahmed
Keyword(s):  
Cell Line ◽  
Reference Group ◽  
Lipid Level ◽  
Biological Evaluation ◽  
Diabetic Rats ◽  
Hypolipidemic Activity ◽  
Albino Rats ◽  
Reference Drug

Objective. The present study assesses the effect ofPupalia lappacea(L.) Juss. (Amaranthaceae) (PL) leaves ethanolic extract on adipocytes, blood glucose level, and lipid level in streptozotocin (STZ) induced diabetic rats.Materials and Methods. Male Albino rats were rendered diabetic by a single moderately sized dose of STZ (45 mg/kg, intraperitoneally) at once before starting the treatment. Animals were divided into five groups: normoglycemic control, diabetic control, reference group (glibenclamide, 5.0 mg/kg), AS001 (250 mg/kg extract), and AS002 (500 mg/kg extract) each containing six animals forin vivostudy. Antidiabetic and hypolipidemic activity of extract were determined byin vivomethod on STZ induced diabetic rats. Antiadipogenic activity was determined byin vitromethod on 3T3-L1 cell line in comparison to simvastatin as reference drug.Result. The extract showed significant fall in fasting serum glucose (FSG), that is, 234.68 and 211.61 mg/dL, in STZ induced diabetic animals for dose groups AS001 and AS002, respectively. ThePLextract also exhibited noteworthy antiadipogenic activity on 3T3-L1 cell line. The value of inhibitory concentration (IC50) ofPLextract to reduce adipocyte cells was found to be 662.14 μg/mL.Conclusion. ThePLextract exhibited significant antiadipogenic, antidiabetic, and hypolipidemic activities.

scholarly journals STUDIES ON IN VITRO ANTIDIABETIC ACTIVITIES OF HOPEA PONGA AND VITEX LEUCOXYLON

2017 ◽  
Vol 9 (2) ◽  
pp. 263 ◽  
Author(s):  
Arun Kashivishwanath Shettar ◽  
Ankala Basappa Vedamurthy
Keyword(s):  
Glucose Uptake ◽  
Aqueous Extract ◽  
Yeast Cells ◽  
Antidiabetic Activity ◽  
Significant Activity ◽  
Soxhlet Apparatus ◽  
Ic50 Value ◽  
Uptake Assay ◽  
Glucose Uptake Assay

<p><strong>Objective: </strong>Evaluating antidiabetic property of <em>Hopea ponga</em> and <em>Vitex leucoxylon</em> extracts by using <em>in vitro</em> assays.</p><p><strong>Methods: </strong>The exhaustive serial extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were evaluated for antidiabetic activity by employing standard <em>in vitro</em> techniques (α-amylase and glucose uptake assay using yeast model in which the effects of extracts on α-amylase and glucose uptake was tested by considering the percentage of inhibition of α-amylase and increase in glucose uptake in yeast cells).</p><p><strong>Results: </strong><em>In vitro</em> antidiabetic studies show that in case of <em>Hopea ponga</em> methanol extract showed comparable antidiabetic activity with percentage of α-amylase inhibition 51.7925±0.92794 % and with IC50 value 96.53 µg and it was less on comparison with standard i.e. 71.0907±0.67796% with IC50 value 70.33 µg and in case of glucose uptake assay aqueous extract showed higher activity over all remaining extracts with percentage of inhibition 49.8100±0.62476% and with IC50 value 250.95 µg. whereas in case of <em>Vitex leucoxylon</em> aqueous extract exhibited significant activity in both performed assays i. e α-amylase inhibition and glucose uptake assay with percentage 54.6147±0.46397% and 57.1337±0.44201% respectively when compared to other solvent extracts.</p><p><strong>Conclusion: </strong>Results confirm that aqueous extract of <em>Vitex leucoxylon</em> exhibited highest antidiabetic activity among all extracts. Additional studies are needed for purification, characterization and structural elucidation of bioactive compounds from aqueous extract and also confirm its antidiabetic property by <em>in vivo</em> studies. The present study provides scientific evidence that the leaves of <em>Hopea ponga and Vitex leucoxylon</em> possess anti-diabetic efficacy. Thus, considering its relative antidiabetic potency, these extracts are the useful therapeutic agents for treating and management of diabetes.</p>

scholarly journals In vivo and in vitro antidiabetic potential of Taraxacum officinale root extracts

2020 ◽  
Vol 33 (3) ◽  
pp. 168-175
Author(s):  
Lana YM. Juee ◽  
Alaadin M. Naqishbandi
Keyword(s):  
Glucose Tolerance ◽  
Glucose Uptake ◽  
Aqueous Extract ◽  
Taraxacum Officinale ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Diabetic Mice ◽  
Phytochemical Study

AbstractTaraxacum officinale F.H. Wigg (Asteraceae) root is traditionally used to treat diabetes, dyspepsia, heartburn, anorexia and hepatitis. In this work, petroleum ether, chloroform, methanol and aqueous extracts of T. officinale root were evaluated for their antidiabetic activity in normoglycemic and alloxan-induced diabetic mice at two concentrations (200 and 400 mg/kg) using antidiabetic and subcutaneous glucose tolerance tests. Herein, in vitro glucose uptake assay was performed using HepG2 and 2-NDBG, while LC-MS/MS was employed for the phytochemical study of the main active constituents in the active extract. In the experiments, T. officinale root aqueous extract (400 mg/kg) showed a significant decrement in blood glucose level (62.33%, p ≤0.05), while other extracts (p >0.05) showed insignificant activity – in alloxan-induced diabetic mice with no apparent effect on the normoglycemic model. The extracts also showed an insignificant reduction in glucose levels (p >0.05) in the subcutaneous glucose tolerance test. However, a significant glucose uptake enhancement (149.6724%, p ≤0.05) was exhibited by the aqueous extract. Phytochemical study of the aqueous extract showed higher total phenolic than total flavonoid content, in which chlorogenic acid, protocatechuic acid, and luteolin-7-glucoside were identified.

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