Synthesis, spectral characterization, DFT and in vitro antibacterial activity of Zn(II), Cd(II) and Hg(II) complexes derived from a new thiosemicarbazide

A new series of mono-nuclear complexes, Cd2+, Zn2+ and Hg2+ derived from 4,4-dimethyl-2,6-dioxo-N-phenylcyclohexanecarbothioamide (HDDPT) have been prepared and characterized. The complexes adopted the molecular formulae; [Hg(DDPT)Cl(H2O)], [Cd (HDDPT) 2Cl2] and [Zn (HDDPT)2Cl2], respectively. IR data showed that HDDPT ligand acts as OS bidentate either in neutral form as in Cd2+, Zn2+ complexes or mononegative chelate in Hg2+complex. An octahedral environment was suggested for Zn2+ and Cd2+ complexes and a tetrahedral arrangement for Hg2+ complex. DFT method we used to confirm the geometries of title compounds and evaluate other energetic parameters such as HOMO, LUMO, hardness, softness and electronegativity. Also, the thermal stability was examined by TGA and DrTGA and the associated thermodynamic parameters of activation were determined using Coats-Redfern and Horowitz-Metzger methods. Also, all compounds were screened for antibacterial activity using ciprofloxacin and gentamicin drugs by inhibition zone diameter method technique.

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In vitro SCREENING ANTIBACTERIAL ACTIVITY OF Bidens pilosa LINNÉ AND Annona crassiflora MART. AGAINST OXACILLIN RESISTANT Staphylococcus aureus (ORSA) FROM THE AERIAL ENVIRONMENT AT THE DENTAL CLINIC

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652014000400011 ◽

2014 ◽

Vol 56 (4) ◽

pp. 333-340 ◽

Cited By ~ 19

Author(s):

Jeferson Junior da Silva ◽

Cláudio Daniel Cerdeira ◽

Juliana Moscardini Chavasco ◽

Ana Beatriz Pugina Cintra ◽

Carla Brigagão Pacheco da Silva ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Toxicity Testing ◽

Inhibition Zone ◽

Bidens Pilosa ◽

Total Absence ◽

Mtt Method ◽

In Vitro Antibacterial Activity ◽

Annona Crassiflora

Currently multiresistant Staphylococcus aureus is one common cause of infections with high rates of morbidity and mortality worldwide, which directs scientific endeavors in search for novel antimicrobials. In this study, nine extracts from Bidens pilosa (root, stem, flower and leaves) and Annona crassiflora (rind fruit, stem, leaves, seed and pulp) were obtained with ethanol: water (7:3, v/v) and their in vitro antibacterial activity evaluated through both the agar diffusion and broth microdilution methods against 60 Oxacillin Resistant S. aureus (ORSA) strains and against S. aureus ATCC6538. The extracts from B. pilosa and A. crassiflora inhibited the growth of the ORSA isolates in both methods. Leaves of B. pilosa presented mean of the inhibition zone diameters significantly higher than chlorexidine 0.12% against ORSA, and the extracts were more active against S. aureus ATCC (p < 0.05). Parallel, toxicity testing by using MTT method and phytochemical screening were assessed, and three extracts (B. pilosa, root and leaf, and A. crassiflora, seed) did not evidence toxicity. On the other hand, the cytotoxic concentrations (CC50 and CC90) for other extracts ranged from 2.06 to 10.77 mg/mL. The presence of variable alkaloids, flavonoids, tannins and saponins was observed, even though there was a total absence of anthraquinones. Thus, the extracts from the leaves of B. pilosa revealed good anti-ORSA activity and did not exhibit toxicity.

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In vitro antibacterial activity of some systemic and topical antihistaminic preparations

Clinical & Investigative Medicine ◽

10.25011/cim.v32i6.10657 ◽

2009 ◽

Vol 32 (6) ◽

pp. 232

Author(s):

Julide Sedef Gocmen ◽

Unase Buyukkocak ◽

Osman Caglayan

Keyword(s):

Antibacterial Activity ◽

Inhibition Zone ◽

Diffusion Method ◽

Blood Levels ◽

Minimal Bactericidal Concentration ◽

High Concentration ◽

Diphenhydramine Hydrochloride ◽

In Vitro Antibacterial Activity ◽

Pheniramine Maleate

Purpose: In vitro antibacterial activity of topical and systemic antihistaminic preparations containing different active substrates against the standard strains of two bacteria was evaluated. Methods: Four topical and 3 systemic preparations containing pheniramine maleate, chlorophenoxamine hydrochloride, and diphenhydramine hydrochloride were studied. The antibacterial activities of these preparations against strains of S. aureus (American Type Culture Collection, ATCC 29213) and S. epidermidis (ATCC 25212) were tested using the disc diffusion method. In addition, the Minimal Innhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of parenteral preparations for these two bacteria were determined. Results: Pheniramine maleate-topical and pheniramine maleate-systemic had no activity against bacteria, but the others showed various rates of activity. Chlorophenoxamine hydrochloride-topical and chlorophenoxamine hydrochloride-systemic were the most effective (P < 0.05). Despite the same active substrate content, diphenhydramine hydrochloride-topical-1 and diphenhydramine hydrochloride-topical-2 yielded different results when they were compared with each other or with the other preparations. Diphenhydramine hydrochloride-topical-2 had a relatively higher rate of activity than diphenhydramine hydrochloride-topical-1. Inhibition zone diameters were 16.9±1.5 mm 12.3±0.5 mm for S .aureus, 17.4±1.0 mm 0 mm for S .epidermidis respectively (P < 0.05). MIC values of parenteral preparations were equal to or above 125 ?g/ml. Conclusion:, MIC values of parenteral preparations were higher than their blood levels in clinical use. Thus, effects of parenteral preparations may not have been reflected in routine clinical practice. However, topical forms have antibacterial activity due to additive substrates and the use of high concentration levels at the site of application. Therefore, in selection of topical forms for appropriate cases, these effects should also be taken into consideration. The antibacterial activity of topical antihistaminic preparations may be useful in certain dermatological pathology.

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In Vitro Antibacterial Activity of Black Soldier Fly (Hermetia Illucens) Larva Extracts Against Gram-Negative Bacteria

Buletin Peternakan ◽

10.21059/buletinpeternak.v43i2.42833 ◽

2019 ◽

Vol 43 (2) ◽

Author(s):

Harlystiarini Harlystiarini ◽

Rita Mutia ◽

I Wayan Teguh Wibawan ◽

Dewi Apri Astuti

Keyword(s):

Antibacterial Activity ◽

Inhibition Zone ◽

Test Method ◽

Diffusion Method ◽

Bacterial Strains ◽

Black Soldier Fly ◽

E Coli ◽

In Vitro Antibacterial Activity ◽

Zone Growth

The aim of this study was to evaluate the in vitro antibacterial activity of Black soldier fly (BSF) larva extract. The BSF larva was extracted using methanol and then tested for antibacterial activity using agar diffusion method (zone growth inhibition). The antibacterial activity was conducted against Salmonella sp. and Escherichia coli, two important bacterial strains in poultry, using six dilution levels (10 mg/ml, 20 mg/ml, 40 mg/ml, 80 mg/ml, 160 mg/ml and 320 mg/ml). All the results were subjected analyze using t-test method. Based on the diameter of the inhibition zone, the BSF larva extract has a strong (P<0.05) antibacterial activity against Salmonella sp. and E. coli when the concentration used 320 mg/ml. In addition, BSF larva extract also contain high amount of lauric acid (49.18%), a saturated fatty acid that has been proven to proposes as antibacterial agent. Therefore, it could be concluded that the BSF larva extract could be used as a candidate for antibacterial substances.

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Antibacterial activity of Brazilian Northeast plants against Corynebacterium pseudotuberculosis

Research Society and Development ◽

10.33448/rsd-v10i11.19875 ◽

2021 ◽

Vol 10 (11) ◽

pp. e509101119875

Author(s):

Maria Emília Alcântara ◽

Antonio Pedro Fróes de Farias ◽

Maria da Conceição Aquino de Sá ◽

Soraya Castro Trindade ◽

Roberto Meyer ◽

...

Keyword(s):

Antibacterial Activity ◽

Small Ruminants ◽

Inhibition Zone ◽

Corynebacterium Pseudotuberculosis ◽

High Prevalence ◽

In Vitro Antibacterial Activity ◽

Agar Well Diffusion Assay ◽

Brazilian Northeast ◽

Diffusion Assay

The production of small ruminants is an important economic activity of the brazilian Northeast, but some diseases have a high prevalence in this region, such as caseous lymphadenitis (CL), caused by Corynebacterium pseudotuberculosis. The treatment of CL is often ineffective, which justifies the search of new active principles from plants, mainly of the region, to have an accessible treatment. For this reason, the present study was carried out to evaluate the in vitro antibacterial activity of Annona squamosa, Azadirachta indica, Allium sativum, Prosopis juliflora and Portulaca oleracea against C. pseudotuberculosis. Agar well diffusion assay (AWD) and broth microdilution - to determine minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) - evaluated the antimicrobial activity. The highest antibacterial potential was obtained by ethanolic extracts of A. indica leaf (MIC 0,12 mg/mL for 2 strains), A. squamosa stalk (MIC 0,55 mg/mL for 3 strains) and shell (MIC 0,6 mg/mL for 3 strains). These extracts also presented the highest inhibition zone in AWD (30 mm, 38 mm and 32 mm, respectively). A. squamosa and A. indica have high antimicrobial potential against C. pseudotuberculosis.

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Phytochemical Analysis and In Vitro Antibacterial Activity of Soymida febrifuga (Roxb.) Juss. and Hemidesmus indicus (L.)

Indian Journal Of Applied Research ◽

10.15373/2249555x/dec2013/14 ◽

2011 ◽

Vol 3 (12) ◽

pp. 57-59 ◽

Cited By ~ 1

Author(s):

K. Riazunnisa K. Riazunnisa ◽

◽

U. Adilakshmamma U. Adilakshmamma ◽

C. Habeeb khadri

Keyword(s):

Antibacterial Activity ◽

Phytochemical Analysis ◽

Hemidesmus Indicus ◽

In Vitro Antibacterial Activity

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In vitro antibacterial activity of some spice extracts against methicillin-resistant Staphylococcus aureus isolated from nose of food handlers

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.03.412 ◽

2020 ◽

Vol 12 (03) ◽

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methicillin Resistant Staphylococcus Aureus ◽

Food Handlers ◽

Methicillin Resistant ◽

In Vitro Antibacterial Activity

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In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

International Journal of Bioassays ◽

10.21746/ijbio.2016.04.0011 ◽

2016 ◽

Vol 5 (04) ◽

pp. 4512

Author(s):

Jackie K. Obey ◽

Anthoney Swamy T* ◽

Lasiti Timothy ◽

Makani Rachel

Keyword(s):

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Ethanolic Extract ◽

Ethanol Extract ◽

E Coli ◽

Zones Of Inhibition ◽

In Vitro Antibacterial Activity ◽

Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

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Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

RENDICONTI LINCEI ◽

10.1007/s12210-021-00991-5 ◽

2021 ◽

Author(s):

Filippo Fratini ◽

Margherita Giusti ◽

Simone Mancini ◽

Francesca Pisseri ◽

Basma Najar ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Diffusion Method ◽

Minimal Bactericidal Concentration ◽

Coagulase Negative Staphylococci ◽

Disk Diffusion Method ◽

In Vitro Antibacterial Activity ◽

High Concentrations ◽

Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

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