Retrospective study of parasitic diseases of cattle through laboratory analysis of clinical samples at Bangladesh Livestock Research Institute, Regional Station, Baghabari, Sirajganj, Bangladesh

Background: The pillars of animal production are sanity, genetics and nutrition. Sanitary control of the herd is important to reduce production costs and maintain health. The Veterinary Clinics Hospital (HCV) of the Federal University of Rio Grande do Sul (UFRGS), located in Porto Alegre - RS, is the place of greatest casuistry of the state, with 20.000 annual attendances, between small and large animals. In view of this scenario, the present study aimed to determine the frequency and characterize the ruminants attended at HCV, in order to help in the future, in the construction of control and prevention strategies of diseases found. Materials, Methods & Results: The documents of ruminant care between January 2007 and May 2018 were searched in the archives of the Hospital of Veterinary Clinics of UFRGS. Data on species, race, sex, age and diagnosis were collected. Diagnoses were classified as conclusive and inconclusive and the cases with conclusive diagnosis were classified according to etiology: infectious and parasitic diseases, metabolic and nutritional diseases, reproductive and obstetric diseases, toxic diseases, traumatic diseases. The prevalence of diseases and characteristics of ruminants attended (species, sex, category) was calculated. During the study period, between January 2007 and May 2018, 341 ruminants were attended, with emphasis on sheep (42%), goats (39%) and cattle (18%). In addition, a camel with foreign body obstruction, a sambar deer with fracture of the first thoracic vertebra and a buffalo with ruminal impaction were attended. The care profile was mapped, with predominance of females (57%) and adults (59%). Most of the animals did not present a defined breed, but among the breeds stands out Texel, of cutting aptitude, in sheeps and Saanen and Holstein, of dairy aptitude, in goats and cattle, respectively. In the retrospective study, the diseases with infectious and parasitic etiology presented the highest prevalence with 27.5% of the attendances, followed by reproductive (17.5%), traumatic (13.5%), metabolic (10%), others (10%) and toxic (2.5%). Sixty-six animals had inconclusive diagnosis (19.5%). Discussion: The highest prevalences were infectious and parasitic diseases, with emphasis on myiasis and verminosis. So, it is perceived that management corrections are sufficient to reduce the number of occurrences of diseases and prophylactic measures such as vaccination protocol, vermifugation and adequate nutritional management are allied in this walk. Among the reproductive diseases, dystocia (42.85%), which is one of the main causes of mortality in the peripartum period, has been highlighted. Dog attack was the major cause of traumas in ruminants and urolithiasis was highlighted in metabolic diseases. In toxic diseases, copper intoxication was the most important. Sheep are extremely sensitive to this intoxication, as they tend to accumulate copper in the organism. The retrospective study made it possible to visualize the panorama of HCV UFRGS visits to ruminants in the last years, mapping the profile and determining the casuistry of the diseases. Studies of hospital veterinary casuistry are rare, mainly involving ruminants. At the end, it is concluded that studies referring to casuistry are important to know the predominant diseases in a specific area and its risk factors considering differential diagnosis and future prevention programs.

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STUDY AND DETERMINATION OF DISEASES AND PESTS OF REPAIR RASPBERRY «BRYANSKOE DIVO» IN THE SOUTH-EAST OF KAZAKHSTAN

10.32014/10.32014/2020.2518-1467.45 ◽

2020 ◽

Vol 384 (2) ◽

pp. 84-90

Author(s):

L. A. Zhailibayeva ◽

S. N. Oleichenko ◽

M. D. Esenalieva ◽

I. Demirtas

Keyword(s):

Causative Agent ◽

Pure Culture ◽

Plant Protection ◽

Soil Samples ◽

Laboratory Analysis ◽

The South ◽

Cultural Characteristics ◽

Purple Spot ◽

Research Institute

The intensity of diseases and pests was determined in LLP "Kazakh Research Institute of Plant Protection and Quarantine named after Zhazken Zhiembayev" in the test center of phytosanitary laboratory analysis for phytopathological and entomological examination. As a result of the analysis of entomological examination on the leaves of raspberries were found pests of the plant. No pests or nematodes were found in soil samples. On the basis of the symptoms of the disease and phytopathological analyses, it was found that the presented samples of raspberries are parasitic with purple spot, the causative agent of Didymella applanala, as well as secondarily inhabited by fungi of the genera Fuzarium, Aspergillius, Alternaria, Penicillium, Mucor. Classical phytopathological methods were used for the phytopathological diagnosis of raspberry disease. Determination of fungal and bacterial microflora was carried out on morphological and cultural characteristics of the colony of fungi and bacteria isolated in pure culture.

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Retrospective Study on Prevalence of Parasitic Diseases of Sheep Diagnosed At Katsina Zonal Veterinary Clinic (2012-2015)

International Journal of Scientific Research in Science Engineering and Technology ◽

10.32628/ijsrset2184118 ◽

2018 ◽

pp. 137-139

Author(s):

Zainab Yahaya

Keyword(s):

Retrospective Study ◽

Statistical Analysis ◽

Prevention And Control ◽

Tick Infestation ◽

Descriptive Statistics ◽

Parasitic Diseases ◽

Parasitic Disease ◽

Veterinary Clinic ◽

Time Period ◽

And Control

A four-year retrospective study was conducted to determine the distribution of parasitic diseases of sheep encountered at the zonal veterinary clinic katsina from 2012 to 2015. A total of 391 cases were recorded during the time period, out of which 115 cases of parasitic diseases were identified. The obtained data were subjected to statistical analysis using descriptive statistics. The result indicated that the overall prevalence of parasitic diseases is 29.40%, with helminthosis (26.60%) as the most prevalent parasitic disease in the study area. Other parasitic diseases of sheep identified during the study are lousiness (0.77%), coccidiosis (1.53%), and tick infestation (0.24%). It is recommended that farmers should be enlightened on the menace of these parasitic diseases, with emphasis on routine deworming, and use of appropriate drugs for prevention and control of these parasitic diseases.

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The Therapeutic Management Of Retroperitoneal Injuries

Acta Medica Marisiensis ◽

10.2478/amma-2013-0032 ◽

2013 ◽

Vol 59 (3) ◽

pp. 140-143

Author(s):

C Crăciun ◽

L Azamfirei ◽

M F Coroș ◽

A Hintea ◽

D Cozma ◽

...

Keyword(s):

Retrospective Study ◽

Early Diagnosis ◽

Minimally Invasive ◽

Retroperitoneal Fibrosis ◽

Surgical Techniques ◽

Parasitic Diseases ◽

Inflammatory Conditions ◽

Minimally Invasive Surgical ◽

Vascular Factor ◽

Surgical Conditions

Abstract Background: In the retro peritoneum different organs of various origins (vascular, nervous, muscular, fascia, etc.) are located. These represent the source of different surgical conditions that cause, even today, serious problems of early diagnosis and treatment. Material and method: We have conducted a retrospective study throughout a period of 8 years (2004-2011). We followed the incidence, the treatment and the evolution of several types of retroperitoneal lesions. Results: In this period 79 cases of retroperitoneal conditions were encountered, representing 0.51% from the total of 15,284 patients admitted and treated within our clinic. The most common cases were those of tumour conditions and retroperitoneal primitive tumours (RPT). Many inflammatory conditions were also encountered. There were a few cases of retroperitoneal overflows or of parasitic diseases and we did not encounter cases of retroperitoneal fibrosis or malacoplaky. Conclusions: In our study the majority of the retroperitoneal conditions were of tumour nature, especially malignant RPTs. Secondary retroperitoneal tumours (adenopathies, metastases, relapses) were less frequent. The excision of the retroperitoneal tumours is delicate, especially due to the vascular factor caused by anatomical conditions and their own vascularisation. On well-chosen cases we can use minimally invasive surgical techniques (laparoscope, punctures and eco-guided drainages), which enable a favorable post-operative evolution.

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One-step RT-PCR Ins214EPE assay for Omicron (B.1.1.529) variant detection v1

10.17504/protocols.io.b2trqem6 ◽

2021 ◽

Author(s):

Nikita Yolshin ◽

Kirill Varchenko ◽

Kseniya Komissarova ◽

Daria Danilenko ◽

Andrey Komissarov ◽

...

Keyword(s):

N Gene ◽

Clinical Samples ◽

Amplification Efficiency ◽

Analytical Sensitivity ◽

Rt Pcr ◽

Pcr Screening ◽

Increased Risk ◽

New Variant ◽

Gene Assay ◽

Research Institute

On 26 November 2021 WHO designated a new variant of concern B.1.1.529 named Omicron. This variant has a large number of mutations, some of which are concerning. Preliminary evidence suggests an increased risk of reinfection with this variant and reduced neutralization by convalescent and vaccinated sera, as compared to other VOCs. Implementation of the high-throughput rRT-PCR screening for Omicron is of great importance for monitoring the spread of this VOC in the population, especially in resource-limited countries lacking sufficient sequencing capacity. Omicron lineage B.1.1.529 (BA.1) has some indels that turned out to be a good target for its detection. In the current protocol, we use ins214EPE for this purpose. Here we describe the 1-step quantitative multiplex RT-qPCR assay consisting of the newly developed Ins214EPE detection set and widely used Hong Kong University N gene assay for SARS-CoV-2 detection (Chu et al., 2020). The assay was validated on the Omicron variant RNA kindly provided by the Pathogenic Microorganisms Variability Laboratory (Dr. Vladimir Guschin, Gamaleya Institute, Moscow, Russia) and RNA from the collection of Smorodintsev Research Institute of Influenza. Omicron RNA specimens were positive in the assay as expected. Negative controls were found negative. 10-fold serial dilutions of Omicron RNA were used to assess ins214EPE assay amplification efficiency. The amplification efficiency was 98,9% (R2 = 0,99). The developed rRT-PCR assay demonstrates high specificity. It was tested on 26 clinical samples (RNA extracted from oropharyngeal swabs) with previously characterized viruses belonging to 8 different SARS-CoV-2 lineages (including Delta B.1.617.2+AY.*) Specific signal was detected only in samples with SARS-CoV-2 Omicron lineage RNA (confirmed by whole-genome sequencing). Specificity was additionally tested on clinical samples positive for other respiratory viruses from the collection of Smorodintsev Research Institute of Influenza - influenza, parainfluenza, human seasonal coronaviruses (OC43, NL63, 229E, HKU1), hRSV, rhinoviruses, bocaviruses, metapneumovirus (33 in total) - with no false-positive results. Ins214EPE Cq 6x B.1.1.7 2x B.1.351 5x AT.1 6x B.1.617.2 4x AY.122 P.1 B.1.1.529 28,72 B.1.1.529 26,29 virus RP Cq SARS Cq Ins214 Cq RSV A 28,76 RSV A 30,56 RSV A 27,70 RSV B 31,49 RSV B 30,98 RSV B 32,33 NL63 32,20 NL63 30,42 NL63 24,95 Oc43 30,34 Oc43 30,69 Oc43 28,64 HKU1 30,06 HKU1 28,30 HKU1 30,73 229E 29,11 229E 32,52 229E 29,37 BoV 32,26 BoV 30,75 BoV 27,25 Rv 32,85 Rv 33,76 Rv 27,75 Piv1 28,63 Piv2 24,72 Piv3 27,01 Piv4 23,90 Adv 29,47 MPV 30,12 HIV A 29,13 HIV A 28,45 HIV A 28,16 39,06 c+ 34,15 26,61 28,44 Analytical sensitivity determination is underway. We consider developed assay to be useful in wide populational RT-PCR screening to assess the spread of Omicron variant.

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Analysis of the causes of the seizure and destruction of carcasses and organs in a slaughterhouse in central Italy in the 2010-2016 period

Italian Journal of Food Safety ◽

10.4081/ijfs.2018.6899 ◽

2018 ◽

Vol 7 (1) ◽

Cited By ~ 2

Author(s):

Margherita Ceccarelli ◽

Elisa Leprini ◽

Paola Sechi ◽

Maria Francesca Iulietto ◽

Luca Grispoldi ◽

...

Keyword(s):

Retrospective Study ◽

Respiratory System ◽

Preventive Measures ◽

Central Italy ◽

Economic Losses ◽

Parasitic Diseases ◽

Post Mortem ◽

Meat Safety ◽

Or Groups ◽

Control And Eradication

Meat safety and hygiene are fundamental issues for producers and endusers. To achieve these goals, the inspection of carcasses and organs at slaughter is critical. The results of post-mortem inspection are the basis for planning preventive measures against consumer risks and for limiting economic losses. In this retrospective study, the causes of the seizure and destruction of carcasses and organs were analysed at a slaughterhouse in central Italy from 2010 to 2016. 436,646 slaughtered animals were taken into consideration, for a total of 61,799 seizures (73.29% pigs, 23.87% cattle, 2.77% sheep and 0.07% horses). The organs or groups of organs that most frequently showed lesions in pigs were liver (72.38%), heart (10.77%) and pluck (10.20%); in cattle, lungs (64.86%), liver (31.20%) and stomachs (11.63%); in sheep, liver (77.15%), pluck (18.70%) and lung (3.80%); in horses, liver (75.56%), kidney (68.89%) and lung (31.11%). Among the diagnoses, parasitic diseases of the liver (ascariasis and distomatosis) were especially frequent in all species, followed by pericarditis and polyserositis in pigs and diseases affecting the respiratory system in cattle. The data obtained show that post-mortem inspection is of fundamental importance for limiting risks for the consumer and ensuring meat safety. It is also evident, even more than ten years after the coming into force of the so-called hygiene package regulations, that the slaughterhouse can still act as an epidemiological observatory to provide the data needed for the development of plans for the control and eradication of the most frequent diseases in the area.

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