scholarly journals Dose Optimization With Pituitary Gland Hormone For Induced Breeding Of Bata Fish (Labeo Bata)

1970 ◽  
Vol 37 (1) ◽  
pp. 70-77
Author(s):  
MI Miah ◽  
AA Mamun ◽  
MMR Khan ◽  
MM Rahman

An experiment on induced breeding of bata fish (Labeo bata) was carried out to determine the optimum dose of pituitary gland hormone. The present study consisted of four treatments namely TI, T2, T3, and T4 with three replications of each. Twenty-four pairs of male and twelve pairs of female were selected from the brood rearing ponds and the average body wt of the female and male were 390±10 and 258±9 g respectively. To observe the effective dose for induced breeding, the females were first injected at the rate of 0.5 (T1), 1.0 (T2), 1.5 (T3) and 2.0 (T4) mg PG/kg body wt. while the second doses were performed at the rate of 4.0 (T1), 4.5 (T2), 5.0 (T3), and 5.5 (T4) mg PG/kg body wt. after 6 h from the first dose. On the other hand the males were administered a single dose of 2 mg PG/kg body wt. at the time of second dose of the female. The ovulation rate, fertilization rate, hatching rate and survival rate were determined. Among the four treatments T2 (1.0 mg PG/ kg body wt.) showed the best result in terms of ovulation rate (89%), fertilization rate (84%), hatching rate (85%) and survival rate (84%). The dose of 1.0 mg PG/kg body wt. can be used in induced breeding of bata fish (L. bata) for the development of hatchery propagation. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9871 BJAS 2008; 37(1): 70-77


2012 ◽  
Vol 9 (2) ◽  
pp. 305-310
Author(s):  
MM Rahman ◽  
MI Miah ◽  
KR Hasan ◽  
SM Farid

Artificial propagation of Mastacembelus pancalus was conducted in order to determine the optimum dose of pituitary  gland (PG) hormone at the hatchery of the Field Laboratory Complex, Faculty of Fisheries, Bangladesh Agricultural  University, Mymensingh. The present study consisted of two trials and each trial had four treatments (T1, T2, T3 and  T4, respectively) with three replications of each. In the experiment single dose and double dose had been used for T1,  T2, T3 and T4, respectively to evaluate the efficiency on ovulation rate, fertilization rate, and hatching rate of M.  pancalus eggs. The hatchlings were reared in aquarium up to 30 days and survival rate was determined. Treatment-3  (T3) of double dose (170mg PG kg-1 and 60mg PG kg-1 body weight of fish for female and male respectively) showed  better results in terms of ovulation rate (90.03±2.56%), fertilization rate (90±0.81%), hatching rate (80±1.84%), and  survival rate (40.00±3.19%) was recorded than other treatments of single and double dose. The water temperature  was recorded 27 to 31°C. In the present study, treatment-3 (T3 in double dose) was the best findings in terms of  ovulation rate, fertilization rate, and hatching rate which can be used in artificial propagation of M. pancalus for the  development of hatchery production. May and June are the suitable months for artificial propagation of the said  species.   DOI: http://dx.doi.org/10.3329/jbau.v9i2.11045   J. Bangladesh Agril. Univ. 9(2): 305–310, 2011



2013 ◽  
Vol 19 (2) ◽  
pp. 111-118
Author(s):  
SM Farid ◽  
MI Miah ◽  
MAB Habib ◽  
MM Rahman

Five hormone doses viz., 40, 60, 70, 85 and 90 mg of Pituitary gland (PG)/kg body endangered Tarabaim (Macrognathus aculeatus) were tested and they were designated as Treatments T1, T2, T3, T4 and T5, respectively. Significantly (p<0.05) higher fertilization (86%) and hatching rates (50%) were obtained from T5 (90 mg of PG extract/kg body weight) than those of other treatments. However, T4 (85 mg of PG extract/kg, body weight) and T3 (70 mg of PG extract/kg body weight) gave the highest (90%) ovulation rate. In May and June, the highest fertilization rate of 82% and 86% respectively and hatching rate 45% and 75%, respectively were obtained at the temperature ranged from 27.0 to 33.0ºC. In conclusion, PG extract at a dose of 90 mg/kg body weight appeared to be the suitable dose for artificial propagation of M. aculeatus, May and June are the suitable months for its artificial propagation.DOI: http://dx.doi.org/10.3329/pa.v19i2.16951 Progress. Agric. 19(2): 111 - 118, 2008



2021 ◽  
Vol 8 (1) ◽  
pp. 171-179
Author(s):  
Md Zahid Hasan ◽  
Md Fakhrul Islam ◽  
Syed Ariful Haque ◽  
Md Saiful Islam ◽  
Md Mokhlasur Rahman ◽  
...  

An experiment on induced breeding of gang magur, Hemibagrus menoda (Hamilton, 1822) using Ovatide hormone was carried out at in the Field Laboratory Complex of Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. This study consists of three treatments each with three replications. The objective of the experiment was to find out the effective dose of Ovatide hormone for induced breeding. A total number of 54 brood fish were used for the experiment among which 36 were male and 18 were female. Brood fish were kept in the ratio of 2♂:1♀ for breeding purpose. Female brood fish were injected at the rate of 7, 5, 3 ml Ovatide/kg body weight while the males were injected with 3, 2.5 and 1.5 ml Ovatide/kg body weight respectively in T1, T2, and T3 at the same time. The brood fish were injected with single dose of Ovatide in all treatments. Ovulation rates were 0%, 100%, 63%; fertilization rates were 0%, 97%, and 90%, and hatching rates were 0%, 95% and 76% in treatments T1, T2, and T3 respectively. Fertilized eggs were incubated for 21-22 h in all the treatments. Higher ovulation rate (100 %), fertilization rate (97%), hatching rate (95%) and survival rate (85%) were found in treatment T2. Therefore, the optimum dose of Ovatide hormone for induced breeding of H. menoda is 2.5 and 5 ml Ovatide/kg body weight of male and female brood fish, respectively. Res. Agric., Livest. Fish.8(1): 171-179, April 2021



2018 ◽  
Vol 13 (3) ◽  
pp. 213
Author(s):  
Jojo Subagja ◽  
Vitas Atmadi Prakoso

Ikan baung (Hemibagrus nemurus) memiliki prospek bagus untuk dikembangkan sebagai komoditas budidaya di Indonesia karena citarasa daging yang enak banyak diminati konsumen. Dewasa ini, peningkatan produksi benih dapat dilakukan melalui pemijahan buatan dengan manipulasi hormonal. Penelitian yang dilakukan bertujuan untuk mengevaluasi produksi induk ikan baung melalui pemijahan buatan dengan dosis penyuntikan GnRH-a berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan November 2017. Induk betina ikan baung yang digunakan berukuran 465,0 ± 71,8 g; dan induk jantan 426,3 ± 46,8 g. Induksi dilakukan dengan penyuntikan hormon GnRH-a dengan tiga dosis yang berbeda pada induk betina (0,3; 0,5; dan 0,7 mL/kg bobot badan), dan ikan jantan dengan dosis 0,4 mL/kg bobot badan. Masing-masing perlakuan terdiri atas tiga ekor induk betina sebagai ulangan. Hormon disuntikkan secara intramuskular, diberikan dua kali penyuntikan 35% dari dosis total pada penyuntikan pertama, dan 65% diberikan pada penyuntikan kedua, dengan interval waktu penyuntikan delapan jam. Parameter yang diamati yaitu jumlah telur ovulasi, derajat pembuahan, derajat penetasan, dan sintasan larva selama tujuh hari. Hasil penelitian menunjukkan bahwa dosis penyuntikan hormon GnRH-a 0,5 mL/kg pada induk ikan baung saat proses pemijahan buatan menghasilkan derajat penetasan yang lebih tinggi dibandingkan dosis 0,3 dan 0,7 mL/kg; serta sintasan larva yang lebih baik dibandingkan dosis 0,7 mL/kg. Sedangkan nilai jumlah telur yang berhasil ovulasi dan derajat pembuahan yang relatif lebih baik ditemukan pada perlakuan dosis 0,7 mL/kg dibandingkan dengan dosis 0,3 dan 0,5 mL/kg.Asian redtail catfish (Hemibagrus nemurus) has a good prospect to be developed as aquaculture commodity in Indonesia. It was proved by the demand of consumers regarding to its delicious flesh taste. Recently, efforts to increase seedling production could be implemented through artificial spawning with hormonal manipulation. This study was conducted to evaluate the production of broodstocks through artificial spawning with different doses of GnRH-a. Study was conducted at Research Institute for Freshwater Fisheries Germplasm, Cijeruk, Bogor in November, 2017. The broodstock used in this study was 465.0 ± 71.8 g for females, and 426.3 ± 46.8 g for males. Induction was performed by injection of GnRH-a hormone with three different doses on female broodstocks (0.3, 0.5 and 0.7 mL/kg of body weight), and male broodstocks with a dose of 0.4 mL/kg of body weight. Each treatment consisted of three females as replications. The hormone was injected intramuscularly, given twice injection: 35% of the total dose is given at the first injection, and the remaining 65% was given at the second injection, with an injection time interval of eight hours. The results showed the observed parameters of number of the ovulated eggs, fertilization rate, hatching rate, and larval survival for seven days, treatment dosage of 0.5 mL/kg showed better results (P<0.05) than the dosage of 0.3 mL/kg and 0.7 mL/kg. Parameters observed were number of ovulated eggs, fertility rate, hatching rate, and survival rate for seven days. The results showed that the dosage of 0.5 mL/kg GnRH-a injection to the broodstock during artificial spawning resulted in higher hatching rate compared to 0.3 and 0.7 mL/kg and better larval survival rate than the dosage of 0.7 mL/kg. Meanwhile, the number of ovulated eggs and fertility rate was relatively better in the treatment of 0.7 mL/kg compared with the dosage of 0.3 and 0.5 mL/kg.



2016 ◽  
Vol 1 (3) ◽  
pp. 553-568 ◽  
Author(s):  
Md Habibur Rahman ◽  
Md Anisur Rahman ◽  
Md Mer Mosharraf Hossain ◽  
Syeda Maksuda Yeasmin ◽  
Abdulla Al Asif

Labeo bata is one of the important minor carps in Bangladesh with great demand as good table fish. The study was conducted to observe the breeding performance at different management practices in Mafatema, Rupali, Modhumoti and Anan fish hatchery and disinfection treatments of water, eggs and fry in Jessore, Bangladesh during 10 March 2014 to 15 May 2015. An improvement in broodstock nutrition and feeding has been shown to greatly improve seed production. Protein and lipid percentage of broodstock diet have been identified as major dietary factors. Protein level was 24.77%, 23.47%, 18.08%, 17.78% and lipid level was 11.07%, 9.50%, 7.74%, 8.14% in Mafatema, Rupali, Modhumoti and Anan fish hatchery respectively. Three concentrations of four chemical-formalin (10, 20, 30 mg/L), malachite green (1, 3, 5 mg/L), NaCl (1, 2, 3 g/L) and methylene blue ( 1, 3, 5 mg/L) treatment regimes and a control were compared for efficacy in treating L. bata eggs to prevent fungus and bacterial infection and improve hatch and survival rate of fry. Highest correlation value between absolute fecundity and body weight (r=.938, p<.05) and total length (r=.891, p<.05) and gonadosomatic index (26.2%) were found in Mafatema fish hatchery among four experimental hatcheries at 24.77% protein and 11.07% lipid level. Better fertilization rate (84.2±5.17%) and hatching rate (82.0±4.30%) were found in Mafatema and Rupali fish hatchery respectively that has significant difference (P<0.05) from that of Modhumoti and Anan fish hatchery at higher protein and lipid level. Lowest deformity rate (6.05+2.65) was observed in Mafatema fish hatchery that was significantly different (P<0.05) from that of Modhumoti fish hatchery. In case of disinfection treatment, methylene blue at 1mg/L bath treatment daily for 4 days showed significantly higher hatching rate (92.33±3.51%) and survival rate (94.33±4.73%).Asian J. Med. Biol. Res. December 2015, 1(3): 553-568



1970 ◽  
Vol 6 (2) ◽  
pp. 361-366
Author(s):  
MFA Molla ◽  
MR Amin ◽  
MN Sarowar ◽  
M Muhammadullah

The paper reports the first incidence of successful induction of breeding in the riverine catfish Rita rita using carp pituitary gland (PG) extract. A breeding trial using four PG doses viz. 80, 100, 120 and 140mg/kg body weight of fish was conducted to optimize the dose of pituitary gland (PG) extract in terms of induction of ovulation in female. The male received a dose of 40mg PG/kg body weight in all cases and was sacrificed for collection of milt. The best performance was shown by the fish treated with 100mg PG/kg body weight in respect of inducing ovulation in females and fertilization and hatching rates of eggs. Keywords: Induced breeding; Rita rita; Ovulation; Fertilization rate; Hatching rate DOI: 10.3329/jbau.v6i2.4835 J. Bangladesh Agril. Univ. 6(2): 361-366, 2008



2017 ◽  
Vol 12 (2) ◽  
pp. 43 ◽  
Author(s):  
Sulaeman Sulaeman ◽  
Ravi Fotedar

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.



2018 ◽  
Vol 13 (2) ◽  
pp. 115
Author(s):  
Lies Setijaningsih ◽  
Jojo Subagja ◽  
Deni Radona ◽  
Brata Pantjara ◽  
Anang Hari Kristanto ◽  
...  

Permintaan ikan belida cukup tinggi untuk memenuhi kebutuhan konsumsi dan bahan baku olahan makanan yang masih mengandalkan tangkapan dari alam. Tingginya eksploitasi mengakibatkan populasi ikan dari tahun ke tahun semakin berkurang dan terancam punah. Teknologi pembenihan ikan belida dengan penggunaan hormon diduga dapat meningkatkan produksi larva untuk keperluan budidaya. Penelitian dilakukan untuk mengevaluasi performasi pemijahan ikan belida secara alami dan buatan melalui induksi hormon HCG dan LHRH analog. Pemijahan alami dan buatan dilakukan menggunakan induk betina sebanyak enam ekor dan jantan tiga ekor (rasio 2:1). Induk yang digunakan berukuran panjang 46 ± 5 cm dan bobot 2.209 ± 623 g. Pemijahan buatan dilakukan dengan induksi hormon HCG dosis 500 IU/kg dan LHRH analog dosis 0,5 mL/kg. Penyuntikan HCG dan LHRH diberikan dengan interval 24 jam. Hasil penelitian menunjukkan pada pemijahan buatan (induksi hormon) diperoleh induk yang memijah sebanyak dua ekor dengan nilai diameter telur (3,1 ± 0,3 mm); fekunditas (282-907 butir); derajat pembuahan (21%-40%); derajat penetasan (56%-75%); dan sintasan (30%-50%); sedangkan pada pemijahan alami diperoleh satu ekor induk yang memijah dengan nilai diameter telur (3,5 ± 0,3 mm); fekunditas (1.616 butir); dan derajat pembuahan (86,7%); selama tiga bulan pengamatan secara intensif dengan frekuensi pemijahan enam kali. Aplikasi teknologi pemijahan dengan menggunakan induksi hormon dapat menghasilkan produk larva pada ikan belida.The demand for fish consumption of Clown Knifefish is considerably high. However, the fish supply for consumption and processed food still relies on wild-caught. Such exploitation has decreased the population of Clown Knifefish to a point that it can become an endangered species. Efforts on the breeding technology of Clown Knifefish through natural and artificial propagation using of hormones have shown promising results. The current study was conducted to evaluate the performance of Clown Knifefish in natural and artificial spawning conditions using the induction of HCG hormone and LHRH analogues. The natural and artificial spawning treatments were conducted using six fish females and three fish males (a ratio of 2:1). The average Broodstocks’ size was 46 ± 5 cm in length and 2,209 ± 623 g in weight. The artificial spawning was conducted with HCG hormone induction (dose of 500 IU/kg body weight) and LHRH analogue (dose: 0.5 mL/kg). HCG and LHRH injections were given within 24 hours interval. The results showed that the artificial spawning (hormone induction) had successfully caused two broodstock to spawn producing eggs with the diameter of 3.1 ± 0.3 mm; fecundity of 282-907 eggs; fertilization rate of 21%-40%; hatching rate of 56%-75%; and survival rate of 30%-50%. In the natural spawning, one broodstock had spawned with the egg diameter of 3.5 ± 0.3 mm; fecundity of 1,616 eggs; and fertilization rate of 86.7%. This study concludes that spawning technology applications using hormonal induction can increase the production larvae of Clown Knifefish broodstock.



2014 ◽  
Vol 1 (1) ◽  
pp. 46
Author(s):  
Prama Hartami ◽  
Muhammad Nur ◽  
Eva Ayuzar

Penelitian ini dilaksanakan selama 9 hari yang bertempat di Laboratorium Hatcheri dan Teknologi Budidaya Universitas Malikussaleh, Aceh Utara,Propinsi Aceh. Penelitian ini bertujuan untuk mengatahui pengaruh penambahan bahan pengencer sperma terhadap fertilitas spermatozoa ikan lele dumbo dan memperoleh jenis bahan pengencer yang terbaik dalam pemijahan ikan lele dumbo. Penelitian ini di gunakan metode eksperimen laboratorium yang di gunakan pola rancangan acak lengkap (RAL) non faktorial dengan 4 perlakuan 3 ulangan. Bahan uji yang digunakan adalah spermatozoa ikan lele dumbo, Ovaprim, Akuadest, Susu steril, Air kelapa muda, Larutan Infus/ NaCl. Parameter yang di ukur ialah daya fertilitas telur, derajat penetasan telur, kelangsungan hidup benih. Berdasarkan hasil penelitian menunjukkan bahwa penggunaan Aquades sebagai pengencer merupakan bahan yang terbaik dimana fertilitas didapatkan sebesar 98.33 %, derajat penetasan telur sebesar 77.28 %, tingkat kelangsungan hidup larva sebesar 93.44 %. Hasil uji analisis statistik untuk semua parameter uji menunjukkan F hitung > dari F Tabel untuk perlakuan Aquades sebagai bahan pengencer. Sementara itu, hasil pengukuran parameter kualitas air seperti suhu 26,07 – 28,15 0C dan pH air 8,67 – 8,95.This research was done for 9 days in Laboratory of Hatchery and Aquaculture Technology Malikussaleh University, North Aceh, Aceh Province. The aim of this research was to know effect of different sperm solutions for effectiveness fertilization rate of Clarias gariepinus. This research used non factorial completely randomized treatment with three replications on each treatments that using aquadest, natural milk, coconut water and NaCl fisiologis as the sperm solutions. The test observed in this research was fertilization rate, hatching rate, and survival rate. Result showed us that the used of aquadest as the sperm solutions got the best goals which was 98,33 % for ferlilization rate, 77,28 % for hatching rate, and 93,44 % for survival rate. Based on statistical analyze also showed that f count > f table in 99.99 % level of trust for the first treatment with value 29.417 > 7.59. Meanwhile the water quality value that measurable during this research was 26.07 – 28.15 0C and 8.67 – 8.95 for pH.



2019 ◽  
Vol 1 (33) ◽  
pp. 68-76
Author(s):  
Son Phuoc Lai ◽  
Tu Thi Cam Le

The trail on artificial reproduction of dwarf gourami(Trichogaster lalius) with HCG at different dosages is to identify the maturation ability of Gophers (Trichogaster lalius) and compare the influence of HCG at different doses on the reproductive efficiency of dwarf gourami.The experiment consisted of 4  treatments (NT): 3000UI(NT1), 4000UI(NT2), 5000UI(NT3), 6000UI/kg(NT4) females. Each treatment was repeated 3 times. The results showed that during the  conditioning process, environmental factors such as temperature and pH were in the appropriate range for the maturation process of broodstock. The highest maturation rate is 40% in females and 45.83% in males. The survival rate in the conditioning process was quite low at 88.33% in males and 66.67% in females,  which however did not affect the results of the experiment. For stimulation to reproduction by HCG at different doses, the fertility rate fluctuates from 33.33% - 66.67% fertility ability ranges from 268.56 to 389.72 eggs / g female, fertilization rate 61.50%, and hatching rate 98.71%. The most appropriate dose of HCG for  broccoli is 4000UI and the highest fertility of the four treatments is 389.72 eggs/g females.



Sign in / Sign up

Export Citation Format

Share Document