scholarly journals Effect of feeding management of broodstock on breeding performance of bata (Labeo bata)

2016 ◽  
Vol 1 (3) ◽  
pp. 553-568 ◽  
Author(s):  
Md Habibur Rahman ◽  
Md Anisur Rahman ◽  
Md Mer Mosharraf Hossain ◽  
Syeda Maksuda Yeasmin ◽  
Abdulla Al Asif
Keyword(s):  
Methylene Blue ◽  
Survival Rate ◽  
Lipid Level ◽  
Gonadosomatic Index ◽  
Dietary Factors ◽  
Hatching Rate ◽  
Breeding Performance ◽  
Fish Hatchery ◽  
Labeo Bata ◽  
Significant Difference

Labeo bata is one of the important minor carps in Bangladesh with great demand as good table fish. The study was conducted to observe the breeding performance at different management practices in Mafatema, Rupali, Modhumoti and Anan fish hatchery and disinfection treatments of water, eggs and fry in Jessore, Bangladesh during 10 March 2014 to 15 May 2015. An improvement in broodstock nutrition and feeding has been shown to greatly improve seed production. Protein and lipid percentage of broodstock diet have been identified as major dietary factors. Protein level was 24.77%, 23.47%, 18.08%, 17.78% and lipid level was 11.07%, 9.50%, 7.74%, 8.14% in Mafatema, Rupali, Modhumoti and Anan fish hatchery respectively. Three concentrations of four chemical-formalin (10, 20, 30 mg/L), malachite green (1, 3, 5 mg/L), NaCl (1, 2, 3 g/L) and methylene blue ( 1, 3, 5 mg/L) treatment regimes and a control were compared for efficacy in treating L. bata eggs to prevent fungus and bacterial infection and improve hatch and survival rate of fry. Highest correlation value between absolute fecundity and body weight (r=.938, p<.05) and total length (r=.891, p<.05) and gonadosomatic index (26.2%) were found in Mafatema fish hatchery among four experimental hatcheries at 24.77% protein and 11.07% lipid level. Better fertilization rate (84.2±5.17%) and hatching rate (82.0±4.30%) were found in Mafatema and Rupali fish hatchery respectively that has significant difference (P<0.05) from that of Modhumoti and Anan fish hatchery at higher protein and lipid level. Lowest deformity rate (6.05+2.65) was observed in Mafatema fish hatchery that was significantly different (P<0.05) from that of Modhumoti fish hatchery. In case of disinfection treatment, methylene blue at 1mg/L bath treatment daily for 4 days showed significantly higher hatching rate (92.33±3.51%) and survival rate (94.33±4.73%).Asian J. Med. Biol. Res. December 2015, 1(3): 553-568

scholarly journals Effects of different dietary levels of vitamin E on the ovarian development and breeding performances of Clarias batrachus (Linnaeus)

1970 ◽  
Vol 7 (1) ◽  
pp. 183-191 ◽  
Author(s):  
A Roy ◽  
MFA Mollah
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Ovarian Development ◽  
Gonadosomatic Index ◽  
Larval Growth ◽  
Clarias Batrachus ◽  
Hatching Rate ◽  
Second Phase ◽  
Breeding Performance ◽  
Significant Difference

To observe the effects of vitamin E on the ovarian development and breeding performances of Clarias batrachus an experiment was conducted in two phases. The first phase concentrated on studying the effects of vitamin E on ovarian development and the second phase on breeding performances. Eighty female C. batrachus broodfish were stocked in 8 cisterns (2.38 × 1.45m2 each) divided into 4 treatments each having two replicates. Each of the cistern was stocked with 10 females. The fish were fed with feed having different levels of vitamin E viz. 0 mg (served as control), 50 mg, 100 mg and 200 mg vitamin E/kg feed for 3 months to study the effects of vitamin E on the growth and ovarian development of C. batrachus broodfish. It was found that growth in terms of body weight of fish fed with 200 mg vitamin E/kg feed (under T4) was higher, while 50 mg vitamin E/kg feed (under T2) gave the poorest result. There was no significant difference among different treatments. Gonadosomatic index and fecundity were highest in fish treated with 100 mg vitamin E/kg feed (under T3), but there was no significant difference among the treatments. After rearing for 3 months fish were used for induction of breeding to study breeding performance. A pituitary gland (PG) dose of 100 mg/kg body weight was used in all treatments. Ovulation rate was 100% in females of all treatments. In case of fertilization and hatching rate the highest result (88.33±2.51 and 82.33±3.05 respectively) was obtained in T2 (50 mg vitamin E / kg feed), but there was no significant difference between T2 and T3. The overall result of this experiment indicates that 50 mg vitamin E/kg feed is the best dose for the breeding performance of female C. batrachus broods. Keywords: Clarias batrachus; Vitamin E; Ovarian development; Breeding performance; Larval growth DOI: 10.3329/jbau.v7i1.4983 J. Bangladesh Agril. Univ. 7(1): 183-191, 2009

scholarly journals Reproductive Performances and Egg Qualities in African Catfish (Clarias gariepinus) Broodstocks Supplemented with Curcumin and Thyroxine Hormone

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 32
Author(s):  
Livana Dethris Rawung ◽  
Damiana Rita Ekastuti ◽  
Muhammad Zairin Junior ◽  
Min Rahminiwati ◽  
Ade Sunarma ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Egg Quality ◽  
Gonadosomatic Index ◽  
Clarias Gariepinus ◽  
Density Lipoprotein ◽  
Cholesterol Concentration ◽  
Hepatosomatic Index ◽  
African Catfish ◽  
Hatching Rate ◽  
Significant Difference

An experiment was designed to study the effects of curcumin and thyroxine hormone supplementation in the diet to improve the reproductive performance of catfish broodstock by improving egg quality that eventually increases the production of seedlings. Catfish used in this experiment were supplemented with curcumin and thyroxine hormone through their feeds for 12 weeks. The results showed that there was no significant difference (p > 0.05) in the hepatosomatic index (HSI), gonadosomatic index (GSI), percentage of gonad maturity, total cholesterol concentration in the spawned eggs, high density lipoprotein (HDL) concentration in the spawned eggs, fertilization rate of spawned eggs, and hatching rate of fertilized eggs.  However, there were significant differences (p < 0.05) in the concentration of vitellogenin in the spawned eggs, egg diameter of the spawned eggs, and the triglycerides contents of spawned eggs. It was concluded that curcumin and thyroxine supplementations of African catfish increased vitellogenin concentrations and diameters of spawned eggs that have great potential to improve the reproductive performance.

scholarly journals REPRODUCTIVE PERFORMANCE OF DOMESTICATED BROODSTOCK OF SILVER PERCH, Bidyanus bidyanus (MITCHELL 1838) AND THE RELATIONSHIP BETWEEN OIL GLOBULE FRAGMENTATION AND EGG QUALITY

2017 ◽  
Vol 12 (2) ◽  
pp. 43 ◽  
Author(s):  
Sulaeman Sulaeman ◽  
Ravi Fotedar
Keyword(s):  
Survival Rate ◽  
Reproductive Performance ◽  
Egg Quality ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
High Fecundity ◽  
Fertilisation Rate ◽  
Significant Difference ◽  
The Relationship

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.

scholarly journals Dose Optimization With Pituitary Gland Hormone For Induced Breeding Of Bata Fish (Labeo Bata)

1970 ◽  
Vol 37 (1) ◽  
pp. 70-77
Author(s):  
MI Miah ◽  
AA Mamun ◽  
MMR Khan ◽  
MM Rahman
Keyword(s):  
Survival Rate ◽  
Pituitary Gland ◽  
Fertilization Rate ◽  
Ovulation Rate ◽  
Optimum Dose ◽  
Dose Optimization ◽  
Hatching Rate ◽  
Brood Rearing ◽  
Labeo Bata ◽  
Average Body

An experiment on induced breeding of bata fish (Labeo bata) was carried out to determine the optimum dose of pituitary gland hormone. The present study consisted of four treatments namely TI, T2, T3, and T4 with three replications of each. Twenty-four pairs of male and twelve pairs of female were selected from the brood rearing ponds and the average body wt of the female and male were 390±10 and 258±9 g respectively. To observe the effective dose for induced breeding, the females were first injected at the rate of 0.5 (T1), 1.0 (T2), 1.5 (T3) and 2.0 (T4) mg PG/kg body wt. while the second doses were performed at the rate of 4.0 (T1), 4.5 (T2), 5.0 (T3), and 5.5 (T4) mg PG/kg body wt. after 6 h from the first dose. On the other hand the males were administered a single dose of 2 mg PG/kg body wt. at the time of second dose of the female. The ovulation rate, fertilization rate, hatching rate and survival rate were determined. Among the four treatments T2 (1.0 mg PG/ kg body wt.) showed the best result in terms of ovulation rate (89%), fertilization rate (84%), hatching rate (85%) and survival rate (84%). The dose of 1.0 mg PG/kg body wt. can be used in induced breeding of bata fish (L. bata) for the development of hatchery propagation. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9871 BJAS 2008; 37(1): 70-77

70 EFFECT OF CRYOPROTECTANT CONCENTRATION ON THE IN VITRO SURVIVAL AND CELL PROLIFERATION OF PORCINE BLASTOCYSTS VITRIFIED USING THE OPEN PULLED STRAW SYSTEM

2008 ◽  
Vol 20 (1) ◽  
pp. 116
Author(s):  
C. Cuello ◽  
J. Sanchez-Osorio ◽  
C. Almiñana ◽  
M. A. Gil ◽  
I. Caballero ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Survival Rate ◽  
Nuclear Antigen ◽  
Proliferation Index ◽  
Hatching Rate ◽  
Hoechst 33342 ◽  
Number Range ◽  
Significant Difference ◽  
In Vitro Survival

The objective of the present project was to study the effect of the concentrations of ethylene glycol (EG) and dimethyl sulfoxide (DMSO) during vitrification on the survival and hatching rates of porcine blastocysts. Embryos were collected by laparotomy from weaned crossbred sows (n = 18), vitrified, and warmed (one-step dilution) as described by Cuello et al. (2004 Theriogenology 62, 1144–1152). Vitrification was performed in different concentrations of EG and DMSO (15%, 16%, and 17% v/v for each cryoprotectant) or in an EG-based medium (40% v/v) using superfine open pulled straws. Fresh and vitrified blastocysts were cultured for 24 h in TCM199 and assessed by stereomicroscopy during the culture. Blastocysts that reformed their blastocoelic cavities after warming, displaying a normal zona pellucida and excellent appearance, were considered viable. The in vitro survival rate was defined as the ratio of viable embryos divided by the total number of embryos cultured. The hatching rate is determined as the ratio of the number of embryos hatched in vitro to the total number embryos cultured. Some vitrified and fresh embryos classified as viable were processed for Hoechst 33342 staining and proliferation cell nuclear antigen (PCNA) inmunolocalization. The proliferation index was defined as the number of PCNA-positive nuclei divided by the total number of nuclei stained with Hoechst 33342. Data were analyzed by ANOVA using the MIXED procedure (SPSS version 11.5; SPSS, Inc., Chicago, IL, USA). Data were expressed as mean values � SEM. The survival rate was similar for fresh and vitrified blastocysts, except for blastocysts vitrified using 15% cryoprotectants, which displayed a lower (P < 0.05) survival rate (84.2 � 4.8%) than fresh blastocysts (94.6 � 5%) and blastocysts vitrified using 40% EG, 16%, or 17% EG-DMSO (88.8 � 4.9, 96.8 � 4.9, and 96.4 � 5%, respectively). Fresh blastocysts showed a higher (P < 0.05) hatching rate (80.7 � 4.5%) than their vitrified counterparts (range: 48.4 � 7.7–55.3 � 7.8%). Vitrified and fresh blastocysts showed similar cell proliferation indexes (range: 75.8 � 3.2–83.7 � 3). When only hatched blastocysts among groups were compared, the proliferation rate decreased (P < 0.05) after vitrification with 17% EG-DMSO. Among vitrification groups, there was no significant difference in the number of total cells. However, vitrified blastocysts had a lower (P < 0.05) total cell number (range: 116.6 � 6.7–124.8 � 6.6) than fresh blastocysts (195.5 � 11.4). In conclusion, under our experimental conditions, the concentration of EG-DMSO can be decreased until 16% in the vitrification medium with no reduction of the in vitro developmental ability of the blastocysts. In addition, a 40% EG-based medium can be used for vitrification with results similar to those achieved using a medium containing 16% EG-DMSO.

122 THE SURVIVAL RATE AND EMBRYONIC QUALITY OF BOVINE PARTHENOGENETIC BLASTOCYSTS POST-VITRIFICATION CRYOPRESERVATION

2010 ◽  
Vol 22 (1) ◽  
pp. 220
Author(s):  
P. Zhang ◽  
Z. W. Wang ◽  
B. Tang ◽  
J. B. Zhang ◽  
Z. Y. Li
Keyword(s):  
Survival Rate ◽  
Sucrose Solution ◽  
Polar Body ◽  
Cell Counting ◽  
Differential Staining ◽  
Hatching Rate ◽  
First Polar Body ◽  
Significant Difference

Cryopreservation of oocytes and embryos is very useful to conservation of animal genetic resources. Recently, parthenogenesis has received considerable attention as a tool for the production of stem cells. Oocytes and embryos undergo considerable morphological changes and functional damage during cryopreservation, and the survival rate is highly depending on species and developmental stage of oocytes and embryos. The aim of this study was to investigate the survival rate and embryonic quality of bovine parthenogenetic blastocysts post-vitrification cryopreservation. Cumulus-oocyte complexes (COC) from slaughterhouse ovaries were aspirated from 2-mm to 8-mm visible follicles with a 5-mL syringe. The COC were matured in vitro for 22 h in bicarbonate-buffered TCM199 media supplemented with 1 mg mL-1 of FSH, 10 mg mL-1 of LH, 1 mg mL-1 of 17-βiestradiol, and 10% FBS. After in vitro maturation, cumulus cells were removed from COC, oocytes with first polar body were activated by 5 μM ionomycin for 5 min and 2 mM 6-DMAP for 4 h. Subsequently, oocytes were co-cultured with bovine fetal fibroblast cells in SOF media supplemented with amino acids (1% NEAA and 2% EAA), 4 mg mL-1 of BSA, and 10% FBS at conditions of 38.5°C and 5% CO2 for 7 to 9 days. The good expanded blastocysts were selected and refrigerated in different vectors [glass micropipettes (GMP) and straws] and same vitrification solution (VS, 20% EG + 20% DMSO). Blastocysts were exposed to VS, loaded on vectors, and plunged into liquid nitrogen within 25 s. After two days refrigeration, vitrified blastocysts were thawed in air for 10 s and placed into 0.25 M sucrose solution for 1 min and 0.15 M sucrose solution for 5 min. Then, the blastocysts were cultured in the SOF medium same as above. Our results showed that when VS was 20% EG + 20% DMSO, the hatching rate (65%) of blastocysts loaded into GMP was significantly higher (P < 0.05) than that (19%) of blastocysts loaded into straws post-vitrification. Meanwhile, vitrified and nonvitrified blastocysts were fixed and stained for differential cell counting as described by Thouas GA et al. 2001 Reprod. Biomed. Online 3, 25-29). By the differential staining, the total cells of nonvitrified parthenogenetic bovine blastocysts were 102.7, which was higher (P > 0.05) than that (86.7) of vitrified blastocysts. Also, no significant difference (P > 0.05) was seen on ratios between vitrified blastocysts (ICM/TE = 0.22) and nonvitrified blastocysts (ICM/TE = 0.25). Our results indicated that a glass micropipette vector was much better than a straw in vitrification cryopreservation of bovine parthenogenetic blastocysts and caused less damage to blastocyst cells. This study lays the foundation for further research to increase the survival rate of vitrification cryopreservation of bovine embryos. This work was supported by the grant from national support plan, China, No. 2007BAD55B03; corresponding author: Ziyi Li.

scholarly journals Identification of causative agent for fungal infection and effect of disinfectants on hatching and survival rate of common carp (C. carpio) larvae

2016 ◽  
Vol 1 (3) ◽  
pp. 578-588 ◽  
Author(s):  
Syeda Maksuda Yeasmin ◽  
Md Anisur Rahman ◽  
Md Mer Mosharraf Hossain ◽  
Md Habibur Rahman ◽  
Abdulla Al Asif
Keyword(s):  
Methylene Blue ◽  
Survival Rate ◽  
Sodium Chloride ◽  
Malachite Green ◽  
Common Carp ◽  
Bacterial Load ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Hatching Rate ◽  
Mycological Examination

Common carp (Cyprinus carpio) is one of the commercially important and commonly cultured fish. In the hatchery intensive incubation leads to microbial overgrowth in C. carpio eggs that hamper egg development, hatchability and larval survivability. The aim of this study is to find out causes of mass mortality in C. carpio eggs during peak- breeding season between March to May 2015 at Mafatema fish hatchery, Chanchra, Jessore sadar upazilla. In the present study three disinfectants with three different concentrations in each such as methylene blue 1, 3 and 5mg/L., malachite green 1, 3 and 5mg/L., sodium chloride 1, 2 and 3g/L were used to observe the hatching rate of fertilized eggs and survival rate of larvae. Bacterial load of culture water was examined during the induced breeding of C. carpio with mycological examination of egg samples with different disinfectants. The total bacterial count fluctuated from 3.4 x 108 CFU/ml to 32.7 x 108 CFU/ml during the period of fertilization to 4days of hatching. The fertilized eggs infected by Saprolegnia spp. were appeared as tuft hairy like balls with a white cottony envelop. Among all the treatment 1mg/L methylene blue, 3mg/L malachite green and 1g/L sodium chloride showed significantly better (P<0.05) hatching rate 95·33±2·08, 88.00±2.64 and 92.33±4.04% respectively. The same concentration of methylene blue, malachite green and sodium chloride showed significantly better (P<0.05) better survival rate 95·00±4.35, 75.00±3.00 and 87.00±6.24% respectively. Finally among all the treatment 1mg/L of methylene blue showed significantly better (P<0.05) hatching and survival rate 95·33±2·08% and 95·00±4.35 % respectively. So 1mg/L of methylene blue is the best disinfectant for C. carpio fertilized egg treatment.Asian J. Med. Biol. Res. December 2015, 1(3): 578-588

scholarly journals Effect of rearing density on reproduction and embryogenesis of zebrafish Danio rerio (Hamilton, 1922)

2017 ◽  
Vol 26 (1) ◽  
pp. 77-82
Author(s):  
Md Golam Rabbane ◽  
Md Mizanur Rahman ◽  
Md Alamgir Kabir ◽  
Md Hasan Faruque
Keyword(s):  
Survival Rate ◽  
Danio Rerio ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Significant Difference ◽  
Rearing Density ◽  
Spawning Success

Effect of rearing density (5, 15, 25, 35 and 45 fish/l2) on reproductive performances such as spawning success, fertilization rate, hatching rate and embryogenesis in terms of per cent survival rate of embryos at cleavage, gastrula, segmentation, pharyngula and hatching were evaluated. Survival, hatching, fertilization rate and per cent survivability of embryos did not differ significantly between the treatments 1, 2 and 3, but showed significant difference (p < 0.05) between 4 and 5. Treatment three (25 fish/l2) had five-folds higher number of successful progeny than treatment one. Results suggest that 25 fish/l2 can be used as optimum rearing density. Dhaka Univ. J. Biol. Sci. 26(1): 77-82, 2017 (January)

scholarly journals BIOLOGI REPRODUKSI DAN TINGKAT KEBERHASILAN PEMIJAHAN IKAN BAUNG Hemibagrus nemurus (Valenciennes, 1840) POPULASI CIRATA DENGAN INKUBASI SUHU BERBEDA

2018 ◽  
Vol 13 (2) ◽  
pp. 131 ◽  
Author(s):  
Deni Radona ◽  
Jojo Subagja ◽  
Vitas Atmadi Prakoso ◽  
Irin Iriana Kusmini ◽  
Anang Hari Kristanto
Keyword(s):  
Reproductive Biology ◽  
Incubation Temperature ◽  
Gonadosomatic Index ◽  
Fertilization Rate ◽  
Maturity Stage ◽  
Hatching Rate ◽  
Water Heater ◽  
Randomized Design ◽  
Significant Difference ◽  
C 30

Ikan baung merupakan salah satu komoditas populer di Indonesia. Dalam pengembangan budidayanya masih diperlukan input teknologi terutama pada proses pembenihan. Penelitian ini bertujuan untuk mengevaluasi karakter biologi reproduksi dan keberhasilannya dalam proses pemijahan pada ikan baung populasi Cirata yang diinkubasi pada suhu 23°C-24°C, 25°C-26°C, 27°C-28°C, dan 29°C-30°C. Inkubasi induk dilakukan pada styrofoam berukuran 45 cm x 35 cm x 25 cm dengan ketebalan 3 cm. Setiap styrofoam diisi satu ekor induk yang matang gonad. Seleksi tingkat kematangan gonad dilakukan secara kanulasi dan induksi hormon menggunakan LHRH analog (0,6 mL/kg). Penyuntikan dilakukan dua kali dengan selang waktu enam jam. Styrofoam diisi air dengan ketinggian 20 cm, dilengkapi tutup pada bagian atas, water heater, dan sistem aerasi. Inkubasi suhu dilakukan secara eksperimental menggunakan rancangan acak lengkap dengan empat perlakuan suhu dan masing-masing perlakuan diulang sebanyak tiga kali. Hasil penelitian menunjukkan suhu optimal inkubasi pada induk yaitu 27°C-28°C dengan waktu laten 8 jam 35 menit, dan derajat ovulasi 100%. Secara statistik inkubasi induk pada suhu 27°C-28°C menunjukkan nilai karakter biologi reproduksi yang berbeda nyata (P<0,05) dibandingkan dengan inkubasi suhu 23°C-24°C dan 25°C-26°C. Nilai biologi reproduksi yang dihasilkan pada perlakuan terbaik tersebut adalah indeks kematangan gonad 8,6 ± 0,5%; fekunditas 23.909 ± 1.473 butir per ekor; derajat pembuahan 85,5 ± 5,5%; derajat penetasan 69,9 ± 5,0%; dan sintasan 72,3 ± 5,8%. Pada inkubasi suhu 27C°-28°C, telur terdistribusi dengan diameter telur rata-rata sebesar 1,5 mm.Asian redtail catfish is one of the most popular fish commodities in Indonesia. However, improvements in its aquaculture technology are still needed, especially in the breeding process. This study was aimed to evaluate the characters of reproductive biology and level of spawning on Asian redtail catfish from Cirata population incubated at different temperature settings of 23°C-24°C, 25°C-26°C, 27°C-28°C, and 29°C-30°C. Broodstock incubation was conducted in styrofoam boxes (sized 45 cm x 35 cm x 25 cm) with a wall thickness of 3 cm. One mature broodstock was placed inside each Styrofoam. Each styrofoam box was previously filled with freshwater of 20 cm deep, equipped with a lid on top, a water heater, and an aeration system. The gonad maturity stage of each broodstock was determined using cannulation, whilst the hormone induction used LHRH hormone analog (0.6 mL/kg). The injection was performed twice within six hours interval. The incubation was arranged in a completely randomized design (CRD) with four temperature treatments with three replicates. The results showed that the optimal incubation temperature for the broodstock was 27°C-28°C with the latent time ovulation of 8 hours 35 minutes and an ovulation rate of 100%. Statistically, the incubation of broodstock at 27°C-28°C showed a significant difference on the reproductive biological character value (P<0.05) compared to temperature ranges at 23°C-24°C and 25°C-26°C. The reproductive biology parameters generated were gonadosomatic index of 8.6 ± 0.5%; fecundity of 23,909 ± 1,473 egg per individual; fertilization rate of 85.5 ± 5.5%; hatching rate of 69.9 ± 5.0%; and survival rate of 72.3 ± 5.8%. At the incubation temperature of 27°C-28°C, the egg was distributed with an average egg diameter of 1.5 mm.

scholarly journals The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

2017 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Mulyati Mulyati ◽  
Suryati Suryati ◽  
Irfani Baga
Keyword(s):  
Survival Rate ◽  
Sea Water ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Inhibitory Ability ◽  
Significant Difference ◽  
Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

Sign in / Sign up

Export Citation Format

Share Document