scholarly journals Induced breeding of the riverine catfish Rita rita

1970 ◽  
Vol 6 (2) ◽  
pp. 361-366
Author(s):  
MFA Molla ◽  
MR Amin ◽  
MN Sarowar ◽  
M Muhammadullah

The paper reports the first incidence of successful induction of breeding in the riverine catfish Rita rita using carp pituitary gland (PG) extract. A breeding trial using four PG doses viz. 80, 100, 120 and 140mg/kg body weight of fish was conducted to optimize the dose of pituitary gland (PG) extract in terms of induction of ovulation in female. The male received a dose of 40mg PG/kg body weight in all cases and was sacrificed for collection of milt. The best performance was shown by the fish treated with 100mg PG/kg body weight in respect of inducing ovulation in females and fertilization and hatching rates of eggs. Keywords: Induced breeding; Rita rita; Ovulation; Fertilization rate; Hatching rate DOI: 10.3329/jbau.v6i2.4835 J. Bangladesh Agril. Univ. 6(2): 361-366, 2008

2013 ◽  
Vol 19 (2) ◽  
pp. 111-118
Author(s):  
SM Farid ◽  
MI Miah ◽  
MAB Habib ◽  
MM Rahman

Five hormone doses viz., 40, 60, 70, 85 and 90 mg of Pituitary gland (PG)/kg body endangered Tarabaim (Macrognathus aculeatus) were tested and they were designated as Treatments T1, T2, T3, T4 and T5, respectively. Significantly (p<0.05) higher fertilization (86%) and hatching rates (50%) were obtained from T5 (90 mg of PG extract/kg body weight) than those of other treatments. However, T4 (85 mg of PG extract/kg, body weight) and T3 (70 mg of PG extract/kg body weight) gave the highest (90%) ovulation rate. In May and June, the highest fertilization rate of 82% and 86% respectively and hatching rate 45% and 75%, respectively were obtained at the temperature ranged from 27.0 to 33.0ºC. In conclusion, PG extract at a dose of 90 mg/kg body weight appeared to be the suitable dose for artificial propagation of M. aculeatus, May and June are the suitable months for its artificial propagation.DOI: http://dx.doi.org/10.3329/pa.v19i2.16951 Progress. Agric. 19(2): 111 - 118, 2008


2012 ◽  
Vol 9 (2) ◽  
pp. 305-310
Author(s):  
MM Rahman ◽  
MI Miah ◽  
KR Hasan ◽  
SM Farid

Artificial propagation of Mastacembelus pancalus was conducted in order to determine the optimum dose of pituitary  gland (PG) hormone at the hatchery of the Field Laboratory Complex, Faculty of Fisheries, Bangladesh Agricultural  University, Mymensingh. The present study consisted of two trials and each trial had four treatments (T1, T2, T3 and  T4, respectively) with three replications of each. In the experiment single dose and double dose had been used for T1,  T2, T3 and T4, respectively to evaluate the efficiency on ovulation rate, fertilization rate, and hatching rate of M.  pancalus eggs. The hatchlings were reared in aquarium up to 30 days and survival rate was determined. Treatment-3  (T3) of double dose (170mg PG kg-1 and 60mg PG kg-1 body weight of fish for female and male respectively) showed  better results in terms of ovulation rate (90.03±2.56%), fertilization rate (90±0.81%), hatching rate (80±1.84%), and  survival rate (40.00±3.19%) was recorded than other treatments of single and double dose. The water temperature  was recorded 27 to 31°C. In the present study, treatment-3 (T3 in double dose) was the best findings in terms of  ovulation rate, fertilization rate, and hatching rate which can be used in artificial propagation of M. pancalus for the  development of hatchery production. May and June are the suitable months for artificial propagation of the said  species.   DOI: http://dx.doi.org/10.3329/jbau.v9i2.11045   J. Bangladesh Agril. Univ. 9(2): 305–310, 2011


2021 ◽  
Vol 8 (1) ◽  
pp. 171-179
Author(s):  
Md Zahid Hasan ◽  
Md Fakhrul Islam ◽  
Syed Ariful Haque ◽  
Md Saiful Islam ◽  
Md Mokhlasur Rahman ◽  
...  

An experiment on induced breeding of gang magur, Hemibagrus menoda (Hamilton, 1822) using Ovatide hormone was carried out at in the Field Laboratory Complex of Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. This study consists of three treatments each with three replications. The objective of the experiment was to find out the effective dose of Ovatide hormone for induced breeding. A total number of 54 brood fish were used for the experiment among which 36 were male and 18 were female. Brood fish were kept in the ratio of 2♂:1♀ for breeding purpose. Female brood fish were injected at the rate of 7, 5, 3 ml Ovatide/kg body weight while the males were injected with 3, 2.5 and 1.5 ml Ovatide/kg body weight respectively in T1, T2, and T3 at the same time. The brood fish were injected with single dose of Ovatide in all treatments. Ovulation rates were 0%, 100%, 63%; fertilization rates were 0%, 97%, and 90%, and hatching rates were 0%, 95% and 76% in treatments T1, T2, and T3 respectively. Fertilized eggs were incubated for 21-22 h in all the treatments. Higher ovulation rate (100 %), fertilization rate (97%), hatching rate (95%) and survival rate (85%) were found in treatment T2. Therefore, the optimum dose of Ovatide hormone for induced breeding of H. menoda is 2.5 and 5 ml Ovatide/kg body weight of male and female brood fish, respectively. Res. Agric., Livest. Fish.8(1): 171-179, April 2021


Our Nature ◽  
2013 ◽  
Vol 10 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Md. Belal Hossain ◽  
Md. Mosaddequr Rahman ◽  
Md. Golam Sarwer ◽  
Md. Yusuf Ali ◽  
Ferdous Ahamed ◽  
...  

The present study compared the effectiveness of the Carp pituitary gland extract (PGE) and the synthetic hormone Ovaprim on spawning success of the stinging catfish, Heteropneustes fossilis during induced breeding. The PGE hormone was administered at 6 mg/kg of body weight for females and 2 mg/kg of body weight for males. In contrast, Ovaprim was administered at 0.3 ml/kg body weight and 0.1 ml/kg of body weight for females and males, respectively. The spawning success was higher in the Ovaprim-induced individuals with better performance recorded at all stages of spawning including latency period, ovulation, fertilization, hatching and incubation period compared to the PGE-induced individuals. In the Ovaprim induced individuals, the latency period occurred within 10 hours while in PG-induced individuals, the latency was after 15 hours. Similarly, ovulation rate was 90% for Ovaprim injected fish but lower 78.7% for PGE injected fish. Higher rate of fertilization was observed in the eggs of Ovaprim treated fishes 86.7% compared to 69.2% in PGE induced fish. On the other hand, hatching rate was 76.9% in eggs spawned from Ovaprim induced individuals compared to 72.7% in PGE induced fish and the incubation period was also shorter at 3.5 h for eggs from Ovaprim-induced fish while the PGE induced fish eggs required a 5-h incubation period. Finally, the results showed that Ovaprim treated fish yielded better results compared the PGE treated fish in terms of ovulation, fertilization and hatching rates of H. fossilis.DOI: http://dx.doi.org/10.3126/on.v10i1.7755 


2018 ◽  
Vol 13 (3) ◽  
pp. 213
Author(s):  
Jojo Subagja ◽  
Vitas Atmadi Prakoso

Ikan baung (Hemibagrus nemurus) memiliki prospek bagus untuk dikembangkan sebagai komoditas budidaya di Indonesia karena citarasa daging yang enak banyak diminati konsumen. Dewasa ini, peningkatan produksi benih dapat dilakukan melalui pemijahan buatan dengan manipulasi hormonal. Penelitian yang dilakukan bertujuan untuk mengevaluasi produksi induk ikan baung melalui pemijahan buatan dengan dosis penyuntikan GnRH-a berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan November 2017. Induk betina ikan baung yang digunakan berukuran 465,0 ± 71,8 g; dan induk jantan 426,3 ± 46,8 g. Induksi dilakukan dengan penyuntikan hormon GnRH-a dengan tiga dosis yang berbeda pada induk betina (0,3; 0,5; dan 0,7 mL/kg bobot badan), dan ikan jantan dengan dosis 0,4 mL/kg bobot badan. Masing-masing perlakuan terdiri atas tiga ekor induk betina sebagai ulangan. Hormon disuntikkan secara intramuskular, diberikan dua kali penyuntikan 35% dari dosis total pada penyuntikan pertama, dan 65% diberikan pada penyuntikan kedua, dengan interval waktu penyuntikan delapan jam. Parameter yang diamati yaitu jumlah telur ovulasi, derajat pembuahan, derajat penetasan, dan sintasan larva selama tujuh hari. Hasil penelitian menunjukkan bahwa dosis penyuntikan hormon GnRH-a 0,5 mL/kg pada induk ikan baung saat proses pemijahan buatan menghasilkan derajat penetasan yang lebih tinggi dibandingkan dosis 0,3 dan 0,7 mL/kg; serta sintasan larva yang lebih baik dibandingkan dosis 0,7 mL/kg. Sedangkan nilai jumlah telur yang berhasil ovulasi dan derajat pembuahan yang relatif lebih baik ditemukan pada perlakuan dosis 0,7 mL/kg dibandingkan dengan dosis 0,3 dan 0,5 mL/kg.Asian redtail catfish (Hemibagrus nemurus) has a good prospect to be developed as aquaculture commodity in Indonesia. It was proved by the demand of consumers regarding to its delicious flesh taste. Recently, efforts to increase seedling production could be implemented through artificial spawning with hormonal manipulation. This study was conducted to evaluate the production of broodstocks through artificial spawning with different doses of GnRH-a. Study was conducted at Research Institute for Freshwater Fisheries Germplasm, Cijeruk, Bogor in November, 2017. The broodstock used in this study was 465.0 ± 71.8 g for females, and 426.3 ± 46.8 g for males. Induction was performed by injection of GnRH-a hormone with three different doses on female broodstocks (0.3, 0.5 and 0.7 mL/kg of body weight), and male broodstocks with a dose of 0.4 mL/kg of body weight. Each treatment consisted of three females as replications. The hormone was injected intramuscularly, given twice injection: 35% of the total dose is given at the first injection, and the remaining 65% was given at the second injection, with an injection time interval of eight hours. The results showed the observed parameters of number of the ovulated eggs, fertilization rate, hatching rate, and larval survival for seven days, treatment dosage of 0.5 mL/kg showed better results (P<0.05) than the dosage of 0.3 mL/kg and 0.7 mL/kg. Parameters observed were number of ovulated eggs, fertility rate, hatching rate, and survival rate for seven days. The results showed that the dosage of 0.5 mL/kg GnRH-a injection to the broodstock during artificial spawning resulted in higher hatching rate compared to 0.3 and 0.7 mL/kg and better larval survival rate than the dosage of 0.7 mL/kg. Meanwhile, the number of ovulated eggs and fertility rate was relatively better in the treatment of 0.7 mL/kg compared with the dosage of 0.3 and 0.5 mL/kg.


2018 ◽  
Vol 13 (2) ◽  
pp. 115
Author(s):  
Lies Setijaningsih ◽  
Jojo Subagja ◽  
Deni Radona ◽  
Brata Pantjara ◽  
Anang Hari Kristanto ◽  
...  

Permintaan ikan belida cukup tinggi untuk memenuhi kebutuhan konsumsi dan bahan baku olahan makanan yang masih mengandalkan tangkapan dari alam. Tingginya eksploitasi mengakibatkan populasi ikan dari tahun ke tahun semakin berkurang dan terancam punah. Teknologi pembenihan ikan belida dengan penggunaan hormon diduga dapat meningkatkan produksi larva untuk keperluan budidaya. Penelitian dilakukan untuk mengevaluasi performasi pemijahan ikan belida secara alami dan buatan melalui induksi hormon HCG dan LHRH analog. Pemijahan alami dan buatan dilakukan menggunakan induk betina sebanyak enam ekor dan jantan tiga ekor (rasio 2:1). Induk yang digunakan berukuran panjang 46 ± 5 cm dan bobot 2.209 ± 623 g. Pemijahan buatan dilakukan dengan induksi hormon HCG dosis 500 IU/kg dan LHRH analog dosis 0,5 mL/kg. Penyuntikan HCG dan LHRH diberikan dengan interval 24 jam. Hasil penelitian menunjukkan pada pemijahan buatan (induksi hormon) diperoleh induk yang memijah sebanyak dua ekor dengan nilai diameter telur (3,1 ± 0,3 mm); fekunditas (282-907 butir); derajat pembuahan (21%-40%); derajat penetasan (56%-75%); dan sintasan (30%-50%); sedangkan pada pemijahan alami diperoleh satu ekor induk yang memijah dengan nilai diameter telur (3,5 ± 0,3 mm); fekunditas (1.616 butir); dan derajat pembuahan (86,7%); selama tiga bulan pengamatan secara intensif dengan frekuensi pemijahan enam kali. Aplikasi teknologi pemijahan dengan menggunakan induksi hormon dapat menghasilkan produk larva pada ikan belida.The demand for fish consumption of Clown Knifefish is considerably high. However, the fish supply for consumption and processed food still relies on wild-caught. Such exploitation has decreased the population of Clown Knifefish to a point that it can become an endangered species. Efforts on the breeding technology of Clown Knifefish through natural and artificial propagation using of hormones have shown promising results. The current study was conducted to evaluate the performance of Clown Knifefish in natural and artificial spawning conditions using the induction of HCG hormone and LHRH analogues. The natural and artificial spawning treatments were conducted using six fish females and three fish males (a ratio of 2:1). The average Broodstocks’ size was 46 ± 5 cm in length and 2,209 ± 623 g in weight. The artificial spawning was conducted with HCG hormone induction (dose of 500 IU/kg body weight) and LHRH analogue (dose: 0.5 mL/kg). HCG and LHRH injections were given within 24 hours interval. The results showed that the artificial spawning (hormone induction) had successfully caused two broodstock to spawn producing eggs with the diameter of 3.1 ± 0.3 mm; fecundity of 282-907 eggs; fertilization rate of 21%-40%; hatching rate of 56%-75%; and survival rate of 30%-50%. In the natural spawning, one broodstock had spawned with the egg diameter of 3.5 ± 0.3 mm; fecundity of 1,616 eggs; and fertilization rate of 86.7%. This study concludes that spawning technology applications using hormonal induction can increase the production larvae of Clown Knifefish broodstock.


Author(s):  
S Hayat ◽  
M Ramzan ◽  
M Zafarullaf ◽  
I Ahmad ◽  
Q Ali ◽  
...  

In present study the Effects of different doses of ovaprim on induced spawning activities of Rita rita were observed with an aim to standardize the dose of ovaprim for successful breeding. In the trial experiment, the females of Rita rita were treated with ovaprim at the rate of 0.5, 0.8 and 1.0ml/kg body weight and males of Rita rita in all the cases were treated at the rate of 0.4ml/kg.  The doses of ovaprim at the rates of 0.5, 0.8, 1.0 ml/kg body weight resulted in 0%, 100% and 100% ovulation respectively. In case of injection of ovaprim at the rate of 0.8ml/kg, the fertilization rate was observed to be about 70% and hatching rate was 58%, which occurred within 20-25 hours after fertilization at water temperature of 270C-290C. In case of injection of ovaprim at the rate of 1.0ml/kg, 18% fertilization rate and 5% hatching rate was observed. The doses of ovaprim at the rates of 0.8ml/kg and 0.4ml/kg body weight for female and male Rita rita respectively was found to be satisfactory for overall breeding performance and the commercially successful production of this fish. Hatchlings from over all experiment were reared up to fingerlings size to about 2 month and were finally stocked into the Chashma lake.


1970 ◽  
Vol 37 (1) ◽  
pp. 70-77
Author(s):  
MI Miah ◽  
AA Mamun ◽  
MMR Khan ◽  
MM Rahman

An experiment on induced breeding of bata fish (Labeo bata) was carried out to determine the optimum dose of pituitary gland hormone. The present study consisted of four treatments namely TI, T2, T3, and T4 with three replications of each. Twenty-four pairs of male and twelve pairs of female were selected from the brood rearing ponds and the average body wt of the female and male were 390±10 and 258±9 g respectively. To observe the effective dose for induced breeding, the females were first injected at the rate of 0.5 (T1), 1.0 (T2), 1.5 (T3) and 2.0 (T4) mg PG/kg body wt. while the second doses were performed at the rate of 4.0 (T1), 4.5 (T2), 5.0 (T3), and 5.5 (T4) mg PG/kg body wt. after 6 h from the first dose. On the other hand the males were administered a single dose of 2 mg PG/kg body wt. at the time of second dose of the female. The ovulation rate, fertilization rate, hatching rate and survival rate were determined. Among the four treatments T2 (1.0 mg PG/ kg body wt.) showed the best result in terms of ovulation rate (89%), fertilization rate (84%), hatching rate (85%) and survival rate (84%). The dose of 1.0 mg PG/kg body wt. can be used in induced breeding of bata fish (L. bata) for the development of hatchery propagation. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9871 BJAS 2008; 37(1): 70-77


2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Sari B. Moria Sembiring ◽  
Jhon H. Hutapea ◽  
A. Muzaki

Fertilization rate of the first generation of coral trout grouper, Plectropomus leopardus from wild broodstock in cultured tank was relatively low of 10-35%. Based on gonad histology analysis of 30 samples, only one male was found in early stage maturation. Therefore, it is needed to increase functional male production to ensure an increase level of egg fertilization. The experiment was conducted for 9 months using coral trout broodstock, from wild stock as control and selected F1 from culture as treatment. Broodstocks were reared in three tanks, each was 100 m3 in volume with 54 fishes/tank. All selected cultured fish (F1) in tank C were implanted with 17a-MT hormone at 50 mg/kg of body weight every month for 3 consecutive months. Wild stock fish in tank A and selected cultured fish (F1) in tank B were not implant as controls. The result showed that the number of male  increased 6 times in implanted by 17a-MT hormone compared to fish without implant only increased 2 times. Therefore, eggs fertilization and hatching rate were significantly increased by implanting F1 broodstocks with hormone17a-MT. Keywords: Functional male, broodstock, Coral trout (Plectropomus leopardus)


2020 ◽  
Vol 20 (1) ◽  
pp. 47
Author(s):  
Ahmad Teduh ◽  
Dinar Tri Soelistyowati ◽  
Odang Carman ◽  
Harton Arfah

The objectives of this research was to evaluate the inheritance of albino slayer phenotype in brushmouth (Hypostomus plecostomus, Linn 1758) through the crosses and reproduction performance. The crossing scheme consisted of reciprocal crosses between albino slayer and albino non slayer (SN, NS) and between albino slayer (SS), each spawning in pairs (1:1) with three replications and twice spawning. The fish used in this study were brushmouth fish (Hypostomus sp.) albino Slayer males 9.07 ± 0.5 cm, albino slayer females 8.96 ± 0.4 cm, albino non slayer males 8.87 ± 0.17 cm, and albino non slayer female 8.86 ± 0.16 cm. Slayer phenotype category was identified based on the caudal length fin with 4.43 ± 0.64 cm. Parameters determined included the phenotype distribution of albino slayer, total number of eggs, fertilization rate, hatching rate and survival rate. The results showed that all crossing schemes produced four phenotypes classes including normal slayer, normal non slayer, albino slayer and albino non slayer. The slayer phenotype of albino brushmouth was highest percentage (75%) in crossing with male albino slayer (SN, SS) and highest number of eggs, while the reciprocal crossing with female albino slayer (NS) produced albino slayer 55% and lower egg count. The ratio of tail fin length to body length in albino slayer phenotype was lower than normal phenotype of broom fish. The fertilization and hatching rates were not significantly different in all crosses; however the number of eggs decreased in the second spawning. The similar phenotype cross between albino slayer phenotypes (SS) resulted the best average of survival rate (92%).


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