scholarly journals The wound healing effect of Iris forentina on full thickness excisional skin wounds: A histomorphometrical study

2015 ◽  
Vol 11 (1) ◽  
pp. 86 ◽  
Author(s):  
Seyed Abbas Mirmalek ◽  
Tina Parsa ◽  
Yekta Parsa ◽  
Soheila Yadollah-Damavandi ◽  
Seyed Alireza Salimi-Tabatabaee ◽  
...  
Keyword(s):  
Herbal Remedy ◽  
Treated Group ◽  
Volume Density ◽  
Skin Wound ◽  
Collagen Bundle ◽  
Vascular Density ◽  
Skin Wounds ◽  
Full Thickness ◽  
Positive Effects ◽  
Mean Diameter

<p class="Abstract"><em>Iris florentina</em> is an ancient herbal remedy which was prescribed by many physicians due to its positive effects on dermatological problems, specifically skin wounds. Hence, we aimed to investigate the effects of this agent on full-thickness excisional skin wounds in laboratory rats. Three groups of rats (180 ± 20 g; n=8) with 1 cm diameter full-thickness excisional skin wound received daily treatment with I. florentina 10% ointment, and a group with no treatment. By using histomorphometrical methods, the amount of fibroblast population, collagen bundle synthesis, and vascularization (length density, mean diameter and volume density of the vessels) were estimated. Outcome of the study exhibited that the increase in fibroblast proliferation rate, collagen bundle synthesis, vascular density and vascular mean diameter in<em> I. florentina-</em>treated group were significant in comparison to the untreated group (p&lt;0.05). Results indicated that <em>I. florentina</em> can be prescribed as a herbal medicine for treatment of skin wounds or be used as an additional agent for today's common medicines.</p><p> </p>

scholarly journals FIBROIN SILK BASED FILMS FOR RAT’S FULL-THICKNESS SKIN WOUND REGENERATION

2016 ◽  
Vol 18 (3) ◽  
pp. 74-84
Author(s):  
L. A. Safonova ◽  
M. M. Bobrova ◽  
O. I. Agapova ◽  
A. Yu. Arkhipova ◽  
A. V. Goncharenko ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Acid Solution ◽  
Skin Wound ◽  
Cytotoxicity Test ◽  
Skin Wounds ◽  
Full Thickness ◽  
Casting Method ◽  
Formic Acid Solution ◽  
Full Thickness Skin ◽  
Thickness Skin

Aimof this study is to research an effect of silk fi broin fi lms fabricated by casting method upon Wistar rat’s full-thickness skin wound regeneration.Materials and methods.4 different kinds of fi lms with protein concentration equal to 20 mg/ml were fabricated: fi lms from silk fi broin aqueous solution, fi lms from silk fi broin formic acid solution, fi lms from silk fi broin aqueous solution containing 30% collagen by weight, fi lms from silk fi broin formic acid solution containing 30% collagen by weight. All kinds of fi lms were fabricated by casting method on polished Tefl on surface. Scanning electron microscopy was applied to research fi lms’ surface structure. Cytotoxicity test of the fi lms was realized on mouse 3T3 fi broblasts model by MTT assay. Manufactured fi lms were utilized to regenerate full-thickness skin wounds in Wistar rats.Results.It was shown that fi lms’ surface was characterized by micro- and nanorelief in the form of roughness. The proliferative activity of mouse 3T3 fi broblasts increased during 7 days of cytotoxicity test. Fabricated fi lms enlarge the regeneration rate of full-thickness Wistar rat skin wounds an average of 25%. Histological analysis indicated structural skin restoration without any infl ammatory tissue.Conclusion.All fabricated fi lms are non-cytotoxic and characterized by appropriate structure for the adhesion and proliferation of fi broblasts. The application of fi lms for full-thickness skin wound regeneration increases its restoration rate which is confi rmed by histological examination. 

scholarly journals Ascorbic acid for the healing of skin wounds in rats

2009 ◽  
Vol 69 (4) ◽  
pp. 1195-1201 ◽  
Author(s):  
CC. Lima ◽  
APC. Pereira ◽  
JRF. Silva ◽  
LS. Oliveira ◽  
MCC. Resck ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Healing Process ◽  
Collagen Fibre ◽  
Experimental Period ◽  
Treated Group ◽  
Healing Time ◽  
Skin Wound ◽  
Control Group ◽  
Skin Wounds ◽  
Skin Repair

BACKGROUND: Healing is a complex process that involves cellular and biochemical events. Several medicines have been used in order to shorten healing time and avoid aesthetic damage. OBJECTIVE: to verify the topical effect of ascorbic acid for the healing of rats' skin wounds through the number of macrophages, new vessels and fibroblast verifications in the experimental period; and analyse the thickness and the collagen fibre organization in the injured tissue. METHODS: Male Rattus norvegicus weighing 270 ± 30 g were used. After thionembutal anesthesia, 15 mm transversal incisions were made in the animals' cervical backs. They were divided into two groups: Control Group (CG, n = 12) - skin wound cleaned with water and soap daily; Treated Group (TG, n = 12) - skin wound cleaned daily and treated with ascorbic acid cream (10%). Samples of skin were collected on the 3rd, 7th and 14th days. The sections were stained with hematoxylin-eosin and picrosirius red for morphologic analysis. The images were obtained and analysed by a Digital Analyser System. RESULTS: The ascorbic acid acted on every stage of the healing process. It reduced the number of macrophages, increased the proliferation of fibroblasts and new vessels, and stimulated the synthesis of thicker and more organized collagen fibres in the wounds when compared to CG. CONCLUSION: Ascorbic acid was shown to have anti-inflammatory and healing effects, guaranteeing a suiTable environment and conditions for faster skin repair.

scholarly journals Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Poonam Shakya ◽  
A. K. Sharma ◽  
Naveen Kumar ◽  
Remya Vellachi ◽  
Dayamon D. Mathew ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Repair ◽  
Healing Process ◽  
Skin Wound ◽  
Skin Wounds ◽  
Full Thickness ◽  
Open Wound ◽  
Full Thickness Skin ◽  
Group I ◽  
Thickness Skin

An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P<0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

scholarly journals TopicalHypericum perforatumImproves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Soheila Yadollah-Damavandi ◽  
Mehdi Chavoshi-Nejad ◽  
Ehsan Jangholi ◽  
Noushin Nekouyian ◽  
Sahar Hosseini ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Healing Process ◽  
Treated Group ◽  
Volume Density ◽  
Diabetic Rat ◽  
Control Group ◽  
Wound Healing Process ◽  
Numerical Density ◽  
Full Thickness ◽  
Closure Rate

Delayed wound healing process is one of the most important concerns in diabetes. Healing of wounds has four phases, namely, hemostasis, inflammation, proliferation, and remodeling. For a successful repair, all four factors must occur properly. Hence, we aimed to evaluate the healing effects ofHypericum perforatum(HP) on full-thickness diabetic skin wounds by using stereological methods. Forty-eight female diabetic rats were randomly divided into four groups (n=12): gel base treated group, HP 5% gel treated group, HP 10% gel treated group, and the control group which received no treatment. A circular 1 cm2full-thickness wound was created on the animal’s neck and wound area was measured every three days. After sacrificing the animals, skin samples were fixed and prepared for stereological evaluations. Based on the results, HP treated group showed faster wound closure rate in comparison with control and vehicle groups (P<0.05). In addition, numerical density of fibroblasts, volume density of collagen bundles, and mean diameter and volume densities of the vessels in HP group were significantly higher than control and vehicle groups. The results of this study showed that HP has the ability to improve tissue regeneration by enhancing fibroblast proliferation, collagen bundle synthesis, and revascularization.

scholarly journals Hollow polydopamine nanoparticles loading with peptide RL-QN15: a new pro-regenerative therapeutic agent for skin wounds

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Huiling Sun ◽  
Ying Wang ◽  
Tiantian He ◽  
Dingwei He ◽  
Yan Hu ◽  
...  
Keyword(s):  
Therapeutic Agent ◽  
Skin Wound ◽  
Skin Wounds ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Oral Ulcers ◽  
Regenerative Therapies ◽  
Injured Skin

Abstract Background Although the treatments of skin wounds have greatly improved with the increase in therapeutic methods and agents, available interventions still cannot meet the current clinical needs. Therefore, the development of new pro-regenerative therapies remains urgent. Owing to their unique characteristics, both nanomaterials and peptides have provided novel clues for the development of pro-regenerative agents, however, more efforts were still be awaited and anticipated. Results In the current research, Hollow polydopamine (HPDA) nanoparticles were synthesized and HPDA nanoparticles loading with RL-QN15 (HPDAlR) that was an amphibian-derived peptide with obvious prohealing activities were prepared successfully. The characterization, biodistribution and clearance of both HPDA nanoparticles and HPDAlR were evaluated, the loading efficiency of HPDA against RL-QN15 and the slow-releasing rate of RL-QN15 from HPDAlR were also determined. Our results showed that both HPDA nanoparticles and HPDAlR exerted no obvious toxicity against keratinocyte, macrophage and mice, and HPDA nanoparticles showed no prohealing potency in vivo and in vitro. Interestingly, HPDAlR significantly enhanced the ability of RL-QN15 to accelerate the healing of scratch of keratinocytes and selectively modulate the release of healing-involved cytokines from macrophages. More importantly, in comparison with RL-QN15, by evaluating on animal models of full-thickness injured skin wounds in mice and oral ulcers in rats, HPDAlR showed significant increasing in the pro-regenerative potency of 50 and 10 times, respectively. Moreover, HPDAlR also enhanced the prohealing efficiency of peptide RL-QN15 against skin scald in mice and full-thickness injured wounds in swine. Conclusions HPDA obviously enhanced the pro-regenerative potency of RL-QN15 in vitro and in vivo, hence HPDAlR exhibited great potential in the development of therapeutics for skin wound healing. Graphic Abstract

scholarly journals Effect of Lyophilized Growth Factors (LGF) Derived from Equine Platelets on Experimentally Induced Skin Wound Healing in Mongrel Dogs

2021 ◽  
Vol 10 (2) ◽  
pp. 75-82
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Healing Process ◽  
Treated Group ◽  
Skin Wound ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Thickness Skin ◽  
The Right ◽  
Tgf Β1

Lyophilized growth factor (LGF) is a novel advanced platelets rich protein growth factor. It has been successfully applied in various fields of regenerative medicine including management of chronic and non-healing wounds and ulcers. Our study aimed to evaluate the effect of subcutaneous infiltration of equine platelets derived lyophilized growth factors on healing of induced full thickness skin wound in dogs. Four healthy mongrel dogs of different ages were used in the current work. Three bilateral critical sized skin wounds were done on the back of each dog. After 24 hours, the right-side wounds were injected in 4 cardinal points around each wound by Saline/lidocaine (control wounds) and simultaneously reconstituted LGF was subcutaneously injected around the left side wounds (LGF treated wounds). Wound contraction was monitored physically and histopathologically. The expression of TGF-β1 and NF-κB was evaluated in wound specimens of both groups. A significant reduction in wound size was recorded in LGF treated group compared to saline treated group. The histopathological scoring of the healing progress revealed significant increase in the degree of re-epithelization bridging the wound edges and collagen deposition in LGF-treated wounds compared to control non-treated wounds along the experimental periods. Additionally, the expression of TGF-β1 and NF-κB showed significant elevation in LGF-treated wounds compared with their expression in control wounds. In conclusion, LGF therapy could be a superior candidate as a regenerative therapy in skin wounds that can positively impact healing process of the cutaneous wounds.

Effect of Fibrin-binding Synthetic Oligopeptide on the Healing of Full-thickness Skin Wounds in Streptozotocin-induced Diabetic Rats

2012 ◽  
Vol 19 (7) ◽  
pp. 1321-1328
Author(s):  
Jae-Eun Chung ◽  
Yun-Jeong Kim ◽  
Yoon-Jeong Park ◽  
Ki-Tae Koo ◽  
Yang-Jo Seol ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jian Zhang ◽  
Yongjun Zheng ◽  
Jimmy Lee ◽  
Jieyu Hua ◽  
Shilong Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Large Animal ◽  
Skin Wounds ◽  
Tgfβ Signaling ◽  
Pulsatile Release ◽  
Ecm Extracellular Matrix

AbstractEffective healing of skin wounds is essential for our survival. Although skin has strong regenerative potential, dysfunctional and disfiguring scars can result from aberrant wound repair. Skin scarring involves excessive deposition and misalignment of ECM (extracellular matrix), increased cellularity, and chronic inflammation. Transforming growth factor-β (TGFβ) signaling exerts pleiotropic effects on wound healing by regulating cell proliferation, migration, ECM production, and the immune response. Although blocking TGFβ signaling can reduce tissue fibrosis and scarring, systemic inhibition of TGFβ can lead to significant side effects and inhibit wound re-epithelization. In this study, we develop a wound dressing material based on an integrated photo-crosslinking strategy and a microcapsule platform with pulsatile release of TGF-β inhibitor to achieve spatiotemporal specificity for skin wounds. The material enhances skin wound closure while effectively suppressing scar formation in murine skin wounds and large animal preclinical models. Our study presents a strategy for scarless wound repair.

scholarly journals Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2554
Author(s):  
Marek Konop ◽  
Anna K. Laskowska ◽  
Mateusz Rybka ◽  
Ewa Kłodzińska ◽  
Dorota Sulejczak ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

Sign in / Sign up

Export Citation Format

Share Document