scholarly journals Electrophoretic pattern of seed proteins in Trifolium L. and its taxonomic implications

2013 ◽  
Vol 20 (1) ◽  
pp. 19-26 ◽  
Author(s):  
N. M. George ◽  
A. Ghareeb ◽  
N. M. Fawzi ◽  
S. Saad
Keyword(s):  
Cluster Analysis ◽  
Numerical Analysis ◽  
Seed Protein ◽  
Electrophoretic Pattern ◽  
Seed Proteins ◽  
Hierarchical Cluster ◽  
Protein Profiles ◽  
Sds Page ◽  
Plant Taxon ◽  
Taxonomic Implications

The taxonomic delimitations of 61 taxa of the genus Trifolium L. belonging to presently accepted five sections, namely Lotoidea, Mistyllus, Vesicaria, Chronosemium and Trifolium are evaluated, based on numerical analysis of their electrophoretic seed protein profiles. The dendrogram, resulted from the hierarchical cluster analysis of SDS-PAGE profiles of seed proteins conform, with some restrictions, to the present splitting of the genus Trifolium into the sections but not into the subsections and series.DOI: http://dx.doi.org/10.3329/bjpt.v20i1.15461Bangladesh J. Plant Taxon. 20(1): 19-26, 2013 (June)

scholarly journals Differences in tomato seed protein profiles obtained by SDS-PAGE analysis

2008 ◽  
Vol 53 (1) ◽  
pp. 13-23
Author(s):  
Elizabeta Miskoska-Milevska ◽  
Blagica Dimitrievska ◽  
Koo Poru ◽  
Zoran Popovski
Keyword(s):  
Seed Protein ◽  
Seed Proteins ◽  
Protein Profiles ◽  
Protein Fragment ◽  
Tomato Seed ◽  
Protein Fragments ◽  
Molecular Weights ◽  
Tomato Seeds ◽  
Sds Page ◽  
Total Seed

The protein profiles of tomato seeds from sub-species ( subsp. cultum Brezh., subsp. subspontaneum Brezh. and subsp. spontaneum Brezh.) were analyzed using SDS-PAGE technique. Electrophoreograms and denzitograms of total, soluble and non-soluble proteins of 31 different samples have showed quantitative and qualitative differences. Qualitative differences in electrophoregrams of total seed proteins refer to protein fragments in zone A (114 kDa, 83 kDa and 65 kDa) and protein fragment in zone C (17 kDa). Qualitative differences in electrophoregrams of soluble seed proteins refer to protein fragment in zone A (94 kDa). Qualitative differences in electrophoregrams of nonsoluble seed proteins refer to protein fragments with molecular weights of: 210 kDa, 85 kDa, 67 kDa and 26 kDa.

scholarly journals Perbandingan Metode Hierarchical Cluster Analysis untuk Analisis Keragaman Hayati Trypanosoma evansi dari Indonesia Berdasarkan Profil Protein (COMPARISON OF HIERARCHICAL CLUSTER ANALYSIS METHODS FOR BIODIVERSITY ANALYSIS OF TRYPANOSOMA EVANSI

2018 ◽  
Vol 18 (4) ◽  
pp. 516
Author(s):  
Didik Tulus Subekti ◽  
Ichwan Yuniarto ◽  
Sulinawati Sulinawati
Keyword(s):  
Cluster Analysis ◽  
Hierarchical Cluster Analysis ◽  
Binary Data ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Hierarchical Cluster ◽  
Trypanosoma Evansi ◽  
Protein Profiles ◽  
Sds Page

Hierarchical Clustering Analysis (HCA) has long been known to be useful for the analysis of biodiversity of microorganisms based on SDSPAGE protein profile (sodium dodecyl sulfate polyacrylamide gel electrophoresis). However, varying methods of HCA consequently produce variability of analysis results and interpretations. Therefore, it is necessary to evaluate and further determine the most appropriate method which could described the biodiversity based on protein profiles of T.evansi isolates from Indonesia. Eleven isolates of T.evansi from different geographic locations were run on SDS PAGE. Furthermore, SDS PAGE protein profiles from eleven isolates were converted into binary data and analyzed using five different methods of HCA i.e. Average Linkage, Complete Linkage, Single Linkage, Ward Linkage and McQuitty Linkage, respectively.Data were also analyzed by multidimensional scaling (MDS) and densitogram. The analysis showed that the dendrogram constructed with Ward Linkage gives the best results and corresponding with densitogram, MDS and able to describe the geographical origins of isolates.

Taxonomic significance of seed protein profiles in Acacia, with special reference to Acacia emilioana

2003 ◽  
Vol 16 (1) ◽  
pp. 35 ◽  
Author(s):  
Alicia L. Lamarque ◽  
Renée H. Fortunato
Keyword(s):  
Seed Protein ◽  
Seed Proteins ◽  
High Concentration ◽  
Protein Patterns ◽  
Qualitative And Quantitative ◽  
Sds Page ◽  
Electrophoretic Data ◽  
Main Protein ◽  
Protein Components ◽  
Total Seed

Total seed proteins of 10 Acacia species were examined by SDS–PAGE. The protein patterns showed qualitative and quantitative differences among the taxa analysed. The main protein components of most species examined had MW's in the range of 38.5–49.0 × 103. Subgenus Aculeiferum differed from subg. Acacia in the presence of a high concentration of proteins in the range of 20–24.5 × 103. Hierarchical clustering of the 10 taxa was undertaken, based on Jaccard distances calculated from electrophoretic data. The species grouped in two main clusters, representing the two subgenera of Acacia that occur in America, namely subg. Acacia and subg. Aculeiferum. The taxonomic placement of Acacia emilioana, a species with uncertain sectional affinity within subg. Aculeiferum, is discussed.

scholarly journals Pollen grains and seed-morphology as related to Biochemical Patterns in five species of genus Ocimum L (Lamiaceae Juss.) of Saudi Arabia

2017 ◽  
Vol 8 ◽  
pp. 42
Author(s):  
Wael Taha Kasem
Keyword(s):  
Seed Protein ◽  
Pollen Grains ◽  
Seed Morphology ◽  
Protein Profiles ◽  
Electrophoretic Patterns ◽  
Sds Page ◽  
Exine Ornamentation ◽  
Seed Characters ◽  
Molecular Patterns ◽  
Scanning Electron

<p>This study aims to investigate the pollen and seed characters by light microscopy (LM) and scanning electron microscope (SEM) as related to biochemical data of seed protein and esterase isoenzymes by SDS-PAGE technique in the five Saudi Arabian <em>Ocimum</em> L. species. The detailed description for pollen and seed in addition electrophoretic patterns were conducted by means of numerical analyses based on thirty-nine parameters. The pollen grains were zonocolpate, hexacolpate, prolate to subprolate. Three types of exine ornamentation were recognized, perforate, reticulate and granulate. Also,  The periclinal, the anticlinal and the boundaries between cells are described by the aid of SEM which exhibited four main distinct types of nutlets sculpture undulate, quirky, circular and straight. Molecular patterns of protein profiles and esterase (EC.3.1.1.1) showed that esterase could be considered as positive markers than protein, minimum and maximum gene / gene expression of esterase isoenzymes are demonstrated. The relationships between the studied taxa were demonstrated as a phenogram. </p>

scholarly journals Use of Seed Protein Profiles to Characterize Peanut Cultivars1

1994 ◽  
Vol 21 (2) ◽  
pp. 152-159 ◽  
Author(s):  
C. M. Bianchi-Hall ◽  
R. D. Keys ◽  
H. T. Stalker
Keyword(s):  
Seed Protein ◽  
Cultivar Identification ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Genetic Material ◽  
Seed Storage Proteins ◽  
Molecular Techniques ◽  
Protein Profiles ◽  
Sds Page

Abstract In the last 10 to 15 yr, the development of biotechnology and molecular techniques has allowed great advancements toward the identification of cultivars among plant species. In legumes, the success of cultivar identification depends on the species under investigation, the type and variability of genetic material found in cultivars, and the technology used for investigations. In this study, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to assess diversity of peanut (Arachis hypogaea L.) seed protein profiles. The objectives of this investigation were a) to assess diversity of protein profiles in peanuts for cultivar identification using SDS-PAGE and b) to determine the extent of variability of seed storage proteins (SSP) among samples of cultivars originating from different locations. The first study included 34 cultivars grown at Lewiston, NC and the second one included nine cultivars grown at six locations. The results of both studies indicated that it is possible to differentiate between subspecies but not to associate a particular profile with only one specific cultivar. Within subspecies, cultivars clustered in more than one group and most cultivars that grouped together were genetically related.

scholarly journals Contribution to the Knowledge of Rocky Plant Communities of the Southwest Iberian Peninsula

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1590
Author(s):  
Ricardo Quinto Canas ◽  
Ana Cano-Ortiz ◽  
Giovanni Spampinato ◽  
Sara del Río ◽  
Mauro Raposo ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Numerical Analysis ◽  
Iberian Peninsula ◽  
Plant Communities ◽  
Hierarchical Cluster Analysis ◽  
Hierarchical Cluster ◽  
Ecological Conditions ◽  
Southern Portugal ◽  
Rocky Habitats

The rocky habitats of southern Portugal are ecosystems with extreme xericity conditions, associated with special abiotic strains. In these unstable ecological conditions, a considerable diversity of plant communities occurs. The objective of this study, carried out in the Algarve and Monchique, and the Mariánica Range biogeographical sectors, is to compare chasmo-chomophytic communities of the southwestern Iberian Peninsula, using a phytosociological approach (Braun–Blanquet methodology) and numerical analysis (hierarchical cluster analysis). From these results, two new communities were identified, Sanguisorbo rupicolae-Dianthetum crassipedis and Antirrhinetum onubensis, as a result of floristic and biogeographical differences from other associations already described within the alliances Rumici indurati-Dianthion lusitani and Calendulo lusitanicae-Antirrhinion linkiani, both included in the Phagnalo saxatilis-Rumicetea indurate class.

scholarly journals Seed protein variability in safflower

2002 ◽  
pp. 29-34
Author(s):  
Dragana Obreht ◽  
Ljiljana Vapa ◽  
Sándor Kis ◽  
Mária-Hajos Takács ◽  
Éva-Bányai Stefánovics ◽  
...  
Keyword(s):  
Protein Content ◽  
Seed Protein ◽  
Seed Proteins ◽  
Carthamus Tinctorius ◽  
Seed Protein Content ◽  
Electrophoretic Patterns ◽  
Sds Page ◽  
The Usa ◽  
Molecular Masses ◽  
Total Seed

Total seed proteins in two safflower species (Carthamus tinctorius L. and C. lanatus L) have been separated by the SDS-PAGE method. Their molecular masses ranged from 120 to 20 kDa. All C. tinctorius genotypes under study exhibited identical electrophoretic patterns which differed from the pattern exhibited by the wild species C. lanatus in the number and position of protein bands. Differences in protein profiles occurred in regions around 60 kDa, from 43 to 36 kDa and around 30 kDa. Statistically significant differences in seed protein content were found among safflower genotypes from different countries as well as among genotypes from the same country but from different sites. The highest seed protein content was found in a genotype originating from the USA.

scholarly journals Identification of some Hibiscus germplasm through numerical analysis

1970 ◽  
Vol 13 (1) ◽  
pp. 49-54
Author(s):  
Nazmul Alam ◽  
Mostafa Kamal Pasha ◽  
Shamsuddin Ahmad
Keyword(s):  
Cluster Analysis ◽  
Numerical Analysis ◽  
Key Words ◽  
Morphological Characters ◽  
Similarity Coefficient ◽  
Numerical Analyses ◽  
Similarity Coefficients ◽  
Taxonomic Relationship ◽  
Plant Taxon

Numerical analyses of 68 morphological characters of 12 varieties/forms belonging to four species of Hibiscus were carried out by calculating Sørensens and Sneath and Sokal similarity coefficients followed by cluster analysis and construction of dendrograms for visual appreciation of taxonomic relationship within this family. The Sørensens similarity coefficient varied between 0.211 and 0.919 and Sneath and Sokal similarity coefficient ranged between 0.142 and 0.890, indicating much variation between the species. Key words: Hibiscus, Numerical analysis, Identification, Cluster analysis, Dendrogram DOI: 10.3329/bjpt.v13i1.593 Bangladesh J. Plant Taxon. 13(1): 49-54, 2006 (June)

scholarly journals Quercus rotundifolia Lam. Woodlands of the Southwestern Iberian Peninsula

Land ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 268
Author(s):  
Ricardo Quinto Canas ◽  
Ana Cano-Ortiz ◽  
Carmelo Maria Musarella ◽  
Sara del Río ◽  
Mauro Raposo ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Numerical Analysis ◽  
Iberian Peninsula ◽  
Plant Communities ◽  
Hierarchical Cluster ◽  
Holm Oak ◽  
Oak Woodlands ◽  
Ecological Features ◽  
New Association ◽  
Quercus Rotundifolia

The holm oak woodlands as ecotonic phytocoenoses occur under different ecological conditions, and frequently representing the climax of edaphoxerophilous series of crests and siliceous rocky areas. In this paper we study the floristic, ecological, and biogeographical differences of the edaphoxerophilous holm oak woodlands of the southwestern Iberian Peninsula, included in the Querco rotundifoliae-Oleenion sylvestris suballiance. Our phytosociological (Braun–Blanquet methodology) and numerical analysis (hierarchical cluster analysis) of three formerly described association and our own samples lead us to propose a new association: Ulici argentei-Quercetum rotundifoliae, growing mostly on semihyperoceanic Monchique Sierran Biogeographic District, on rocky slopes and outcrops derived from schists and greywackes. Moreover, we present an overview of ecological features and the diversity of plant communities occurring in the serial dynamic of the thermophile holm oak woodlands of the southwest of the Iberian Peninsula.

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