scholarly journals Etiology of UTI and Frequency of ESBL Producing Bacteria Isolated from Patients of Dhaka Medical College Hospital with Their Antimicrobial Susceptibility Pattern

2017 ◽  
Vol 25 (1) ◽  
pp. 26-31
Author(s):  
Shahin Ara Begum ◽  
Shabeen Afreen ◽  
Farook Ahamed ◽  
Akhtaruzzaman Chowdhury ◽  
Mohammad Jobayer ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bacterial Infections ◽  
Medical College Hospital ◽  
Urine Samples ◽  
Resistant Bacteria ◽  
Coagulase Negative Staphylococcus ◽  
Biochemical Tests ◽  
College Hospital ◽  
Medical College ◽  
Tract Infections

Background: Urinary tract infections (UTIs) are the most common of all bacterial infections and occur at any time in the life of an individual. ESBL producing bacteria particularly Escherichia.coli is one of the most common causes of UTIs both in community and healthcare associated settings. Emergence of multidrug resistance (MDR) is quite alarming and cause failure of empirical treatment of UTIs. As a result increase the morbidity and mortality rate in the developing countries like Bangladesh.Objective: The objective of this study was to find out the bacteria causing UTI from urine culture and detection of ESBL producing Esch.coli and K.pneumoniae with their anti- microbial susceptibility pattern.Materials and Methods: A total of 1750 urine samples were collected from patients with symptoms and suspected UTI. Clean catch mid-stream urine samples were collected from indoor and outdoor patients of Dhaka Medical College Hospital during January 2015 to July 2015.Urine specimens were cultured in 5% Blood agar and MacConkeys agar media. The isolated bacteria were identified by gram staining and biochemical tests. Antimicrobial susceptibility and detection of ESBL were done by disc diffusion method.Result: Out of 1750 urine samples, 403(23.03%) were positive by culture. Among the culture positive cases, 216 (53.59%) were female and 187 (46.41%) were male. The most common isolated bacteria were Esch.coli 295(73.20%) followed by Pseudomonas aeuroginosa 85(21.09%), K.pneumoniae10(2.48%), Proteus spp. 4(.99%), Acinetobacter spp. 5(1.2%), Coagulase negative Staphylococcus (CONS) 4(.99%). Among the isolated Esch.coli and K.pneumoniae, ESBL producing bacteria were 202 (68.47%) and 5 (50%) respectively. All the isolated bacteria showed low level susceptibility to all antibiotics that are used during the study period.Conclusion: Treatment of UTIs is difficult when caused by multidrug resistant bacteria. Analysis of culture and sensitivity data should be done periodically to identify ESBL producing bacteria for proper treatment of UTIs.J Dhaka Medical College, Vol. 25, No.1, April, 2016, Page 26-31

scholarly journals Characterization of extended spectrum β-Lactamase producing bactieria isolated from urinary tract infections

2019 ◽  
Vol 45 (1) ◽  
pp. 23-33
Author(s):  
Munawar Sultana ◽  
Anowar Khasru Parvez ◽  
Khandokar Fahmida Sultana ◽  
Sanjoy Kumer Mukharje ◽  
M Anwar Hossain
Keyword(s):  
Urinary Tract ◽  
Multidrug Resistant ◽  
Medical College Hospital ◽  
Urine Samples ◽  
Rrna Gene ◽  
College Hospital ◽  
Unique Challenge ◽  
Medical College ◽  
Extended Spectrum ◽  
Tract Infections

Background: A unique challenge for clinical microbiologists, clinicians, infection control professionals is to deal with extended-spectrum β-lactamase (ESBL) producing pathogens. The study was aims to isolate ESBL producing bacteria from urine samples of Urinary Tract Infection (UTI)-patients and to analyses their phenotypic and genotypic characteristics. Methods: A total of 90 urine samples from UTI patients were collected from Enam Medical College Hospital and Gonoshastha Medical College Hospital, Savar, Dhaka, Bangladesh; between May-2012 to August-2012. A total 75gram negative isolates were retrieved and screened for ESBL production by the Double Disk Diffusion Synergy Test (DDST). Isolates with ESBL phenotype were further characterised by antibiotic susceptibility testing, PCR and sequencing of β-lactamase genes. Results: Cultural and biochemical assay combined with 16S rRNA gene based phylogenetic identification confirmed that Escherichia spp. were predominant pathogens associate with UTI (41%), and the rest were distributed within the genus Enterobacter spp. 26%, Klebsiella spp. 21% , and Pseudomonas spp.10%. Total 31 isolates were phenotypically confirmed as ESBLs through DDST. The multidrug resistant (MDR) and ESBL producing bacteria showed high resistance to cefotaxime (96%), cefixime (90%) and imipenem (32%). PCR reaction was carried out targeting the genes blaTEM, blaCTX. Dominant ESBL class was CTX-M (65%) followed by TEM (52%). All ESBL isolates except 7 possesses multiple plasmids indicating possibility of both chromosomal and plasmid inheritance of ESBLs. Conclusions: This study shows a high prevalence of ESBL producing MDR in UTI patients among these two hospitals of Bangladesh indicating the necessity of alternative therapeutic intervention. Bangladesh Med Res Counc Bull 2019; 45: 23-33

scholarly journals Isolation and Antimicrobial Susceptibility Pattern of Urinary Escherichia Coli in Dhaka Medical College Hospital, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 40-44
Author(s):  
Tashmin Afroz Binte Islam ◽  
SM Shamsuzzaman ◽  
Irin Rahman ◽  
Faria Ferdousi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Test Reaction ◽  
College Hospital ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern

Background: Escherichia coli is the major bacterial pathogen being isolated and reported from urine, globally. For these uropathogens the therapeutic management becomes limited, so knowledge about the antimicrobial resistance pattern of these agents at a specific area may help the doctors to choose correct treatment regimen. The aim of the current study was to detect the common uropathogens from urine and the antimicrobial susceptibility pattern of Escherichia coli in Dhaka Medical College Hospital (DMCH).Methods: Samples were collected over a period of 12 months from July 2011 to June 2012 from the patients of DMCH irrespective of age and sex. Samples were cultured in blood agar and MacConkey agar media and organisms were identified by different biochemical tests such as oxidase test, reaction in MIU and simmon's citrate media and different sugar fermentation tests. Data were analyzed using Microsoft Excel (2007).Results: From total 300 urine samples, 177 (59%) Gram-positive and Gram-negative bacteria were isolated. Among them, Esch. coli was the most predominant 112 (63.28%) organism followed by Enterobacter spp. (11.87%). The prevalence of Esch. coli was significantly higher in females (63.39%) of younger age group than in males (36.61%). Esch. coli was highly sensitive to imipenem (100%) followed by ceftriaxone (62.50%), ceftazidime (55.36%) and azitromycin (35.72%) respectively. Low sensitivity patterns were found against doxycycline (13.39%), co-trimoxazole (16.07%), amoxiclav (21.43%) and ciprofloxacin (23.22%) respectively.Conclusion: The result of this study provides insight into the high proportion of multidrug resistant Escherichia coli and creates a need to report them routinely in laboratories.Anwer Khan Modern Medical College Journal Vol. 7, No. 1: Jan 2016, P 40-44

scholarly journals Antibiotic Sensitivity Pattern of Uropathogens at Khwaja Yunus Ali Medical College Hospital

KYAMC Journal ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 610-613
Author(s):  
Abdullah Akhtar Ahmed ◽  
Ahmed Nawsher Alam ◽  
Rejwana Fatmi ◽  
Md Mashiur Rahman ◽  
Marufa Sultana Munia ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Medical College Hospital ◽  
Urine Samples ◽  
College Hospital ◽  
Health Care Center ◽  
E Coli ◽  
Medical College ◽  
Staphylococcus Saprophyticus ◽  
Sensitivity Pattern ◽  
Culture Positive

Increasing resistance among urinary tract pathogens to conventional drugs is found alarming worldwide. The aim of this study was to obtain data on susceptibility patterns of isolated uropathogens from urine samples of patients attending at Khwaja Yunus Ali Medical College Hospital, Enayetpur, Sirajgonj, Bangladesh a tertiary health care center of remote area to currently used antimicrobial agents. A total of 656 urine samples were studied of which 163 (24.8%) were culture positive. Among 163 culture positive cases all uropathogenic isolates were identified. Among the isolates, E. coli was the most predominant 116 (71.1%) followed by Pseudomonas aeruginosa 29 (17.8%). Staphylococcus saprophyticus 12 (7.4%) and Klebsiella pneumoniae 4 (2.4%) and Acenatobacter sp. 2 (1.2%). The majority of isolated E. coli were sensitive to Meropenam (58.6%) and Amikacin (57.7%) followed by gentamicin (52.6%), amoxiclav (43.1%), ciprofloxacin (42.2%). Sensitivity & resistance rate in Staphylococcus saprophyticus were same (50%) in Cephradine . Where complete (100%) resistanc e was shown to Ampicillin in Pseudomona s aeruginosa , Klebsiella pneumoniae and Acenatobacter sp. However most of isolates were poorly sensitive to ampicillin (1.7%), erythromycin (2.5%), cefuroxin (3.4%) and amoxicillin (9.5%). This area-specific monitoring studies aimed to gain knowledge about the type of pathogens responsible for UTIs and their resistance patterns may help the clinician to choose the right empirical treatment. Meropenem, amoxiclav, amikacin, gentamicin, second-generation of both cephalosporins and quinilones are found the most sensitive against the common uropathogens which might be used in the treatment of UTI.KYAMC Journal Vol. 6, No.-2, Jan 2016, Page 610-613

scholarly journals Bacteriological Etiology of Flood Affected Diarrhoeal Patients Admitted in Dhaka Medical College Hospital and Antibiogram of the isolates

2016 ◽  
Vol 2 (1) ◽  
pp. 13-17
Author(s):  
Abdur Rahman ◽  
Aliya Rashid ◽  
Shaheen Ara Begum ◽  
Aktheruzzaman Chowdhury ◽  
Shahin Ara Begum ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Age Groups ◽  
Medical College Hospital ◽  
Diffusion Method ◽  
Aeromonas Species ◽  
Biochemical Tests ◽  
College Hospital ◽  
E Coli ◽  
Medical College ◽  
Almost All

Diarrhoea is a self-limiting  disease  caused by various  enteropathogens and antimicrobial  therapy  is  an  important adjunct  to  fluid  therapy  in  the  management of the  cases.  We have studied  the  flood-affected  diarrhoeal  (post-flood diarrhoea) patients  admitted in  Dhaka Medical College Hospital  during  August to September,  2007.  A total  of ll4 faecal  samples  were  collected  from  the  patients of all age  groups,  and  investigated  for  bacterial  enteropathogens. Specimens  were cultured  in  appropriate  media and  identification  of the  organisms  showing  growth  was done  by relevant  biochemical  tests.  In addition, the isolates of Vbrio cholera wer confirmed  using  pollvalent  antisera. Sensitivity of the isolated organisms was done by disc diffusion method.  Among the isolated bacterial enteropathogens, majority was V. cholera (46, 40.3%). Other  isolates  included Escherichia coli (18,  23.0%),  Aeromonas species  (8,10.O%)  and V parahaemolyticus  (5,  7.0%). No pathogenic bacteria were isolated from 27 of the cases.  No Salmonella or Shigella was detected in any case. Majority of the cases  (24, 52.0%)  infected  with V cholerae  were  children  having  less than  L0 years of  age. Most of the isolates of V cholerae (77.7%) were sensitive to Ciprofloxacin.  Almost all of the V cholerae isolates (97.90 ) were resistant to Tetracycline, followed by Co-trimoxazloe (93,5%)  and  Nalidixic  acid (59.2%).  All of the E. coli isolates (100.0%) were sensitive to Ceftriaxone, followed by Ceftazidime (88.8%) and Ciprofloxacin (77.7oh).  Thus it  can  be concluded  that  Ciprofloxacin and Ceftriaxone  can  be considered  as the drugs  ofchoice  for  the  treatment  of  post-flood  diarrhoea  along  with proper  rehydration  therapy.Bangladesh  J Med Microbiol2008;  02 (01):13-17DOI: http://dx.doi.org/10.3329/bjmm.v8i1.21929

scholarly journals Antibiotic resistance pattern of bacteria causing urinary tract infection in a private medical college hospital, Dhaka

2017 ◽  
Vol 16 (1) ◽  
pp. 42-47
Author(s):  
Mahmuda Siddiqua ◽  
Ahmed Nawsher Alam ◽  
Sonia Akter ◽  
Reena Saad Ferdousi
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
College Hospital ◽  
E Coli ◽  
Medical College ◽  
Tract Infections ◽  
Resistance Rates

Background and objective: Urinary tract infection (UTI) is one of the frequently seen infections both in the world and in our country as well. Escherichia coli (E. coli) aremost frequently isolated in complicated or uncomplicated, nosocomial or community acquired urinary tract infections. To guide the empirical therapy, the resistance pattern of E. coli responsible was evaluated throughout the period in this study.Material and Method: Urine samples from outpatient / inpatient department of Ibn Sina Medical College Hospital between 1st January, 2015 and 31st December, 2015 were retrospectively analyzed. Presence of ?105 colony forming units/ml in urine culture was considered as significant for UTI. Isolated bacteria were identified by standard laboratory techniques and antibiotic susceptibility testing was performed by Kirby-Bauer disk diffusion method using Clinical Laboratory Standard Institute (CLSI) criteria.Result: A total of 271(13.4%) uropathogens were isolated. Overall E. coli accounted for 180 (66.42%) of all isolates. Resistance rates of E. coli to antimicrobial agents was demonstrated to be as follows: cefuroxime 82%, nalidaxic acid 74%, azithromycin 56%, cefotaxime52%, ceftazidime50%, cefixime 47%, cotrimoxazole 43%, ceftriaxone 41%, ciprofloxacin38%, amoxicillin- clavulanic acid 31%, cefepime30%, and low resistance which ranges from 9 to 1% included gentamycin 9%, meropenem 3%, imipenem2%, nitrofurantoin2% and amikacin 1%.Conclusion: As resistance rates show regional differences, it is necessary to regularly monitor regional resistance pattern to determine the appropriate empiric antibiotic treatment. The national antibiotic usage policies must be reorganized according to data obtained from these studies.Bangladesh Journal of Medical Science Vol.16(1) 2017 p.42-47

scholarly journals Monitoring Antimicrobial Susceptibility in bacterial isolates causing Urinary Tract Infections in a Tertiary Hospital in Kathmandu

2020 ◽  
Vol 19 (1) ◽  
pp. 133-141
Author(s):  
Vivek Kumar Singh ◽  
Mahesh Kumar Chaudhary ◽  
Megha Raj Banjara ◽  
Reshma Tuladhar
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Tertiary Care ◽  
Urine Samples ◽  
Care Hospital ◽  
Biochemical Tests ◽  
Tract Infections

   Urinary tract infection is the most common infection in females worldwide. One in three women experiences at least one episode of urinary tract infection during their lifetime. The objective of this study was to determine the etiology and antimicrobial profile of urinary tract infection. A cross-sectional study was conducted at a tertiary care hospital in Nepal. Thirteen hundred clean catch mid-stream urine samples were tested through standard microbiological techniques. The isolates from urine samples were identified from biochemical tests. Antibiotic susceptibility testing was performed through the Kirby-Bauer disc diffusion technique following CLSI guidelines. The prevalence of urinary tract infection was found at 24.23%. Escherichia Coli was a predominant etiological agent followed by Staphylococcus aureus. The majority of the infection was found between the age group 21-40, with females mostly infected. Most of the microorganisms were isolated from emtergency, obstetrics-gynecology, and nephrology wards. Most of the isolates were resistant to ampicillin, whereas the majority of the gram-positive isolates were resistant to penicillin.A large number of isolates were found to be sensitive to Gentamycin and nitrofurantoin. Routine antimicrobial susceptibility testing of the isolates causing urinary tract infection is recommended to reduce antimicrobial resistance and for the  proper treatment of infection.

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