scholarly journals Preparation and In vitro Evaluation of Mucoadhesive Tablets of Montelukast Sodium

2018 ◽  
Vol 20 (2) ◽  
pp. 123-131
Author(s):  
Farzana Akter Bithi ◽  
Tushar Saha ◽  
Nusrat Ahmed ◽  
Ikramul Hasan ◽  
Md Selim Reza
Keyword(s):  
Ex Vivo ◽  
Pharmaceutical Journal ◽  
Compression Technique ◽  
Mucoadhesive Polymers ◽  
Montelukast Sodium ◽  
Mucoadhesive Tablets ◽  
Ft Ir ◽  
Eudragit Rlpo ◽  
Ft Ir Spectroscopy

Mucoadhesive tablets of montelukast sodium were prepared in order to release the drug for a prolonged period of time so as to reduce the frequency of administration. Direct compression technique was applied using the mucoadhesive polymers which were Methocel K4M CR, Methocel K15M CR, Methocel K100M CR and Eudragit RLPO. Highest percent release of drug after 8 hours was 76% for Methocel K4M CR, 72.13% for Methocel K15M CR, 65.68% for Methocel K100M CR, 65.53% for the combination of Methocel K15M CR and Eudragit RLPO. Higuchi, Krosmeyer-Peppas was the best fitted model for drug release. The Methocel K15M CR containing drug showed good mucoadhesive strength. The highest ex-vivo mucoadhesive strength and ex-vivo residence time were observed with Methocel K15M CR and Eudragit RLPO combination. Fourier transform infrared (FT-IR) spectroscopy revealed the compatibility of drug with the polymers.Bangladesh Pharmaceutical Journal 20(2): 123-131, 2017

The Development of Pemetrexed Diacid-Loaded Gelatin-Cloisite 30B (MMT) Nanocomposite for Improved Oral Efficacy Against Cancer: Characterization, In-Vitro and Ex-Vivo Assessment

2020 ◽  
Vol 17 (3) ◽  
pp. 246-256
Author(s):  
Kriti Soni ◽  
Ali Mujtaba ◽  
Md. Habban Akhter ◽  
Kanchan Kohli
Keyword(s):  
Drug Release ◽  
Oral Delivery ◽  
Ex Vivo ◽  
Confocal Laser ◽  
Release Mechanism ◽  
Scanning Calorimetry ◽  
Sem Images ◽  
Cloisite 30B ◽  
Ft Ir

Aim: The intention of this investigation was to develop Pemetrexed Diacid (PTX)-loaded gelatine-cloisite 30B (MMT) nanocomposite for the potential oral delivery of PTX and the in vitro, and ex vivo assessment. Background: Gelatin/Cloisite 30 B (MMT) nanocomposites were prepared by blending gelatin with MMT in aqueous solution. Methods: PTX was incorporated into the nanocomposite preparation. The nanocomposites were investigated by Fourier Transmission Infra Red Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Scanning Electron Microscope (SEM) X-Ray Diffraction (XRD) and Confocal Laser Microscopy (CLSM). FT-IR of nanocomposite showed the disappearance of all major peaks which corroborated the formation of nanocomposites. The nanocomposites were found to have a particle size of 121.9 ± 1.85 nm and zeta potential -12.1 ± 0.63 mV. DSC thermogram of drug loaded nanocomposites indicated peak at 117.165 oC and 205.816 oC, which clearly revealed that the drug has been incorporated into the nanocomposite because of cross-linking of cloisite 30 B and gelatin in the presence of glutaraldehyde. Results: SEM images of gelatin show a network like structure which disappears in the nanocomposite. The kinetics of the drug release was studied in order to ascertain the type of release mechanism. The drug release from nanocomposites was in a controlled manner, followed by first-order kinetics and the drug release mechanism was found to be of Fickian type. Conclusion: Ex vivo gut permeation studies revealed 4 times enhancement in the permeation of drug present in the nanocomposite as compared to plain drug solution and were further affirmed by CLSM. Thus, gelatin/(MMT) nanocomposite could be promising for the oral delivery of PTX in cancer therapy and future prospects for the industrial pharmacy.

scholarly journals CHEMICAL MODIFICATION, CHARACTERIZATION AND EVALUATION OF MUCOADHESIVE POTENTIALITY OF ASSAM BORA RICE STARCH

2017 ◽  
Vol 9 (9) ◽  
pp. 132 ◽  
Author(s):  
Abdul Baquee Ahmed ◽  
Iman Bhaduri
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Sustained Release ◽  
Chemical Modification ◽  
Ex Vivo ◽  
Rice Starch ◽  
Modified Starch ◽  
Native Starch ◽  
Ft Ir

Objective: The objective of the present study was to chemical modification, characterization and evaluation of mucoadhesive potentiality of Assam bora rice starch as potential excipients in the sustained release drug delivery system. Methods: The starch was isolated from Assam bora rice and esterified using thioglycolic acid and characterized by Fourier transform infrared spectroscopy (FT-IR), Differential scanning calorimetry (DSC) and Nuclear magnetic resonance (NMR). The 10% w/v gel formulation based on modified bora rice starch loaded with irinotecan (0.6%) was prepared and evaluated for various rheological properties, ex-vivo mucoadhesion using goat intestine and in vitro drug release study in phosphate buffer pH 6.8.Results: The chemical modification was confirmed by FT-IR and NMR studies with the presence of the peak at 2626.74 cm-1 and a singlet at 2.51 respectively due to–SH group. Ex-vivo mucoadhesion studies showed 6.6 fold increases in mucoadhesion of the modified starch with compared to native starch (46.3±6.79g for native starch; 308.7±95.31g for modified starch). In vitro study showed 89.12±0.84 % of drug release after 6 h in phosphate buffer pH 6.8 and the release kinetics followed Non-Fickian diffusion.Conclusion: The modified Assam bora rice starch enhanced a mucoadhesive property of the native starch and thus, can be explored in future as a potential excipient for the sustained release mucoadhesive drug delivery system.

Synthesis, in vitro inhibition effect of novel phthalocyanine complexes as carbonic anhydrase and paraoxonase enzyme inhibitors

2020 ◽  
Vol 24 (08) ◽  
pp. 1047-1053
Author(s):  
Emre Güzel ◽  
Barış Seçkin Arslan ◽  
Kübra Çıkrıkçı ◽  
Adem Ergün ◽  
Nahit Gençer ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Enzyme Inhibitors ◽  
Inhibition Effect ◽  
H Nmr ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
First Time ◽  
Ft Ir Spectroscopy ◽  
C Nmr

The preparation and assessment of carbonic anhydrase and paraoxonase enzyme inhibition properties of 3-(2-(5-amino-4-(4-bromophenyl)-3-methyl-1H-pyrazol-1-yl)ethoxy)phthalonitrile (2) and its nitrogen-containing non-peripheral phthalocyanine derivatives (3 and 4) are reported for the first time. The new phthalonitrile and its phthalocyanine derivatives have been elucidated by FT-IR spectroscopy, 1H-NMR, [Formula: see text]C-NMR, mass and UV-vis spectroscopy. The results demonstrated that all synthesized compounds moderately inhibited carbonic anhydrase and paraoxonase enzymes. Among the compounds, the most active ones were found to be compound 4 for PON (Ki : 0.14 [Formula: see text]M), compound 3 for hCA I (Ki : 22.52 [Formula: see text]M) and compound 1 for hCA II (Ki : 13.62 [Formula: see text]M).

scholarly journals Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5326 ◽  
Author(s):  
Adekemi Titilayo Adesulu-Dahunsi ◽  
Kumaraswamy Jeyaram ◽  
Abiodun Isiaka Sanni ◽  
Kolawole Banwo
Keyword(s):  
Lactobacillus Plantarum ◽  
Antioxidant Activities ◽  
Scale Up ◽  
Food Industries ◽  
Ft Ir ◽  
Experimental Values ◽  
Central Composite ◽  
Ft Ir Spectroscopy ◽  
Significant Factors

Lactobacillus plantarum YO175 and OF101 isolates from Nigerian traditional fermented cereal gruel ‘ogi’, were investigated on the basis of their capability to produce exopolysaccharide (EPS) on sucrose modified deMan Rogosa Sharpe medium (mMRS). Functional groups analysis of the EPSs produced (EPS-YO175 and EPS-OF101) by Fourier-transform infrared (FT-IR) spectroscopy revealed the presence of –OH, C=O and C–H groups. The chemical composition of EPS-YO175 and EPS-OF101 showed the presence of 87.1% and 80.62% carbohydrates and 1.21% and 1.47% protein. For maximum EPS yield, three significant factors were optimized using central composite design and response surface methodology, the predicted maximum EPS produced was 1.38 g/L and 2.19 g/L, while the experimental values were 1.36 g/L and 2.18 g/L for EPS-YO175 and EPS-OF101. The EPS samples showed strong antioxidant activities in-vitro. The scale-up of the production process of the EPS will find its potential application in food industries.

scholarly journals FT-IR Spectroscopy Based Evaluation of Changes in Primary Metabolites of Amsonia orientalis after In vitro 6-benzylaminopurine Treatment

2016 ◽  
Vol 44 (1) ◽  
pp. 209-214 ◽  
Author(s):  
Arda ACEMİ ◽  
Sevgi TÜRKER-KAYA ◽  
Fazıl ÖZEN
Keyword(s):  
Ir Spectroscopy ◽  
Plant Growth ◽  
Natural Populations ◽  
Molecular Data ◽  
Primary Metabolites ◽  
Bern Convention ◽  
Ft Ir ◽  
Common Plant ◽  
Ft Ir Spectroscopy

Amsonia orientalis Decne. (syn. Rhazya orientalis (Decne.) A. DC.) is only cultivated as an ornamental plant but also has a medicinal merit. The natural populations of the species were taken under conservation as per the Bern Convention by European Council. As one of the most common plant growth regulators and synthetic agricultural fertilizers, the effects of 6-benzylaminopurine (BAP) at different concentrations on primary metabolites of in vitro propagated A. orientalis have been investigated by using Fourier transform infrared (FT-IR) spectroscopy. Among all applied concentrations, 2.0 mg l-1 BAP resulted in increment of hydrogen bonded polysaccharide and proteins concentrations, lipid structure and membrane fluidity. Treatments of 2.0 and 4.0 mg l-1 BAP increased the existence of ferulic acid which also indicates the linkage of xylan and both cellulose and hemicellulose content. There was a significant decrease in the area of the peak which indicated the C=O stretching of proteins and pectins for 0.5 and 1.0 mg l-1 BAP while there was a significant increase for 2.0 and 4.0 mg l-1 of BAP treatments. The evaluation of molecular data from FT-IR spectroscopy as in the current study might help to estimate different physiological changes at the molecular level in plants depending on plant growth regulator and/or fertilizer applications. Thus, this FT-IR based method can be applied to in vitro propagation studies and also to studies focused on effects of pesticide and/or herbicide applications on plants to understand the metabolic changes in target species.

scholarly journals Preparation and Characterization of Polyamidoamine G2.0-Hematin as a Biocatalyst for Fabricating Catecholic Gelatin Hydrogel

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Tan Phuoc Ton ◽  
Thi Yen Nhi Tran ◽  
Le Hang Dang ◽  
Kim Thi Hoang Nguyen ◽  
Phuong Doan ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
In Vitro Study ◽  
Pamam Dendrimer ◽  
Relative Activity ◽  
Oxidation Reactions ◽  
Enzyme Mimicking ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

In this study, we report that an enzyme-mimicking biocatalyst polyamidoamine (PAMAM) dendrimer G2.0-hematin (G2.0-He) was fabricated successfully. The chemical structure of G2.0-He was verified by 1H NMR and FT-IR spectroscopy. G2.0-He exhibited a size distribution from 11.6 ± 1.7   nm to 12.5 ± 2.9   nm and a zeta potential from 32.5 mV to 25.6 mV along with the enhancement of the hematin conjugation degree. The relative activity of G2.0-He was evaluated based on pyrogallol oxidation reactions at pH = 7 . The results showed that G2.0-He was more stable than horseradish peroxidase (HRP) enzyme in high H2O2 concentrations. The HRP-mimic ability of G2.0-He was also confirmed by the catalyzation when preparing catecholic gelatin hydrogels under mild conditions. Moreover, our results also revealed that these hydrogels performed with excellent cytocompatibility in an in vitro study and could be used as a potential scaffold for adhesion and proliferation of fibroblast cells. The obtained results indicated that G2.0-He is a suitable platform for altering the HRP enzyme in several biomedical applications.

scholarly journals Synthesis of New Nucleoside Analogues From Benzimidazole and Evaluation of Their Antimicrobial Activity

2016 ◽  
Vol 13 (2) ◽  
pp. 317-332
Author(s):  
Baghdad Science Journal
Keyword(s):  
Sodium Methoxide ◽  
Aromatic Aldehydes ◽  
Nucleoside Analogues ◽  
Prepared Compound ◽  
Gram Negative ◽  
Ft Ir ◽  
Anomeric Carbon ◽  
Base Moiety ◽  
Ft Ir Spectroscopy

Our goal in this research, some new nucleoside analogues was synthesized. Starting from ?-D glucose which was converted to per acetylated ?-D gluco pyronoside then converted to active from(1-Bromo Sugar (2) as a sugar moiety.The base moiety 2-substituted benzimidazole was prepared from condensation of phenylene diamine with different aromatic aldehydes, which were subjected to amino alkylation via Mannich reaction forming new nucleobase derivatives. Condensation of nucleobase with bromo sugar through nucleophilic substitution of anomeric carbon with nitrogen forming new protected nucleoside analogues then hydrolyzed with sodium methoxide in methanol to obtain our target, the free nucleoside analogues. All prepared compound were identified by FT-IR Spectroscopy and some of them with H1 –NMR and C13-NMR Spectroscopy. The synthesized nucleoside analogues were screened for their antibacterial activity in vitro against four types of bacteria including, Bacillus Staphylococcus, aureus (Gram Positive), E.Coli and Pseudoman as (Gram Negative).Also were screened against four types of Fungi (Aspergines flurs, Aspergillus fumgntnts, Aspergillus niger and pencillum).

scholarly journals Characterization of Chitosan from Shell of Snail (Achatina Fulica F) and Its Antibacterial Activity againts Staphylococcus aureus

Biota ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 22-31
Author(s):  
Umar Umar ◽  
Surahmaida Surahmaida ◽  
Rio Alta ◽  
Rizka Surya Ningrum
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Deacetylation Degree ◽  
Ft Ir ◽  
Amine Groups ◽  
One Way Anova ◽  
Test Result ◽  
Ft Ir Spectroscopy

The shell of snail contains chitin that can be transformed into chitosan through four stages, which are deproteination, demineralization, depigmentation, and deacetylation. Chitosan can be utilized as an antibacterial agent because it has amine groups. In this research, chitosan that prepared from the snail shell will be analyzed using FT-IR spectroscopy to determine the deacetylation degree (DD). The antibacterial activity test result of Staphylococcus aureus will be carried out on chitosan with various concentrations using in-vitro Kirby Bauer method and will be analyzed using One Way ANOVA with confidence level at 95%. The variations concentration of chitosan used is 300, 400, 500, 600, and 700 ppm. The result shows that the deacetylation degree of chitosan is 63.09% and the optimum concentration of chitosan that can inhibit the Staphylococcus aureus bacteria is 500 ppm with diameter inhibition is 34.33 mm.

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