scholarly journals Characterization of Phytoconstituents and Exploration of Antioxidant and Free Radical Scavanging Activities of Citrus assamensis Leaf

2018 ◽  
Vol 17 (1) ◽  
pp. 29-36
Author(s):  
Mohammad Shahriar ◽  
Mohiuddin Ahmed Bhuiyan ◽  
Md Sohel Rana
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Leaf Extracts ◽  
Total Antioxidant ◽  
Ethanol Extracts

The aim of the current investigation was to identify bioactive secondary metabolites and compare the phytoconstituents and antioxidant profile of different leaf extracts of Citrus assamensis. Preliminary phytochemical screening of chloroform extract of C. assamensis indicated the presence of alkaloids, phytosterols, phenols, tannins, glycosides, saponins and flavonoids, whereas the methanol and ethanol extracts were found to contain no saponin. The screenings of the plant revealed that, the amount of phenols was higher in methanol extract, while the ethanol extract was found to be rich in flavonoid and total antioxidant capacity. Methanol extract of C. assamensis leaf showed potent nitric oxide and hydrogen peroxide scavenging activity having IC50 value of 137.85 and 85 μg/ml, respectively. The chloroform extract was found to be highly effective in DPPH free radical scavenging, reducing power, cupric reducing antioxidant capacity and superoxide dismutase activity when compared to the standard. In case of catalase assay, all the C. assemensis leaf extracts showed highest activity at 50μg/ml. In most cases, lowest concentration of test groups showed significant (**p<0.01 & *p<0.05) peroxidase activity.Dhaka Univ. J. Pharm. Sci. 17(1): 29-36, 2018 (June)

scholarly journals Antihyperglycaemic and antioxidant activities of Crataeva adansonii DC. ssp. adansonii leaves extract on ICR mice

2020 ◽  
Vol 10 (1-s) ◽  
pp. 30-38
Author(s):  
Kokou Atchou ◽  
Povi Lawson-Evi ◽  
Kossi Metowogo ◽  
Batomayena Bakoma ◽  
Kwashie Eklu-Gadegbeku ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Total Antioxidant

Crataeva adansonii DC. ssp. adansonii is a medicinal plant belong to Capparidaceae family, used traditionally by Togolese to treat diabetes mellitus. The aim of this study was to evaluate the antihyperglycaemic and antioxidant activities of the plant leaves. The effect of hydroethanolic extract of C. adansonii ssp. adansonii leaves was assessed in vivo on blood glucose levels in fasting normal and glucose loaded hyperglycemic (oral glucose tolerance test) mice. The phytochemical screening has been done by coloring tests in chemistry. In vitro antioxidants assays had been performed by 2, 2'-azobis 2 amidinopropane dihydrochloride (AAPH) free radical scavenging assay, Ferric reducing antioxidant power (FRAP) assay, Fe2+ chelating assay and total antioxidant capacity (TAC) quantification. After 30 min glucose loading (4 g/kg), the extract 500 mg/kg was showed a significant (p < 0.001) decreased of the peak of blood glucose compared to the hyperglycemic control. The two doses of extract (250 and 500 mg/kg) did not act on the basic blood glucose compared to the normoglycemic control. Phytochemical screening revealed a presence of flavonoids, tannins, alkaloids and sterols. AAPH free radical scavenging activity, reducing power, Fe2+ chelating activity and total antioxidant capacity have shown dose-dependent antioxidant capacity and reducing power of extract compared to ascorbic acid and Butylated hydroxyl Toluene used as reference drug. These finding prove that C. adansonii ssp. adansonii leaves can be used in the treatment of diabetes mellitus and its complications related to oxidative stress and support the use of the plant in traditional medicine in diabetes treatment.  Keywords: Crataeva adansonii, antihyperglycaemic, phytochemical, antioxidant.

scholarly journals Antioxidant potential of common leafy vegetables in Bangladesh

2015 ◽  
Vol 44 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Sheikh Julfikar Hossain ◽  
M Shahina Sultana ◽  
M Iftekharuzzaman ◽  
S Amir Hossain ◽  
M Abu Taleb
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Leafy Vegetables ◽  
Basella Alba ◽  
Total Antioxidant ◽  
Ethanol Extracts ◽  
Gallic Acid Equivalent

Amount of polyphenols, flavonoids, anthocyanins in ethanol extracts and antioxidant activity of both ethanol and lipophilic extracts of common leafy vegetables in Bangladesh were compared. Among the 12 leafy vegetables, Ipomoea aquatica Forssk. showed the highest content of total polyphenols (38.9 mg gallic acid equivalent (GAE)/g extract) and flavonoids (23.2 mg (+)-catechin equivalent (CE)/g extract). Highest anthocyanins content was found in Amaranthus gangeticus L. (1.15 ?mol/g extract) followed by Pisum sativum L. (0.85 ?mol/g extract). Ethanol extracts of Brassica campestris L., Enhydra fluctuans Lour., I. aquatica, Lagenaria siceraria (Mol.) Standl. and P. sativum exhibited high DPPH free radical scavenging activity with IC50 of 104.2, 85.5, 26.9, 125 and 68.5 ?g/ml, respectively whereas for the same lipophilic pentane extracts of Basella alba L. showed the lowest (68.4 ?g/ml) IC50 followed by I. aquatica (70.4 ?g/ml), E. fluctuans (75.7 ?g/ml), L. siceraria (78.3 ?g/ml) and B. campestris (80.6 ?g/ml). I. aquatica also showed the highest NO free radical scavenging followed by B. campestris and B. alba. Highest reducing power (O.D. = 1.7) was observed for I. aquatica followed by P. sativum (O.D. = 1.12), B. alba (O.D. = 0.86) and L. siceraria (O.D. = 0.72) at 400 ?g/ml extract. They also displayed high total antioxidant capacity. Therefore, the top five potential leafy vegetables consist of both hydrophilic and lipophilic antioxidant(s), the order being I. aquatica > B. campestris > B. alba > P. sativum > and L. siceraria. DOI: http://dx.doi.org/10.3329/bjb.v44i1.22723 Bangladesh J. Bot. 44(1): 51-57, 2015 (March)

scholarly journals In Vitro Antioxidant and Thrombolytic Activities of Bridelia Species Growing in Bangladesh

2013 ◽  
Vol 5 (2) ◽  
pp. 343-351
Author(s):  
A. Anjum ◽  
M. A. Sikder ◽  
M. R. Haque ◽  
C. M. Hasan ◽  
M. A. Rashid
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Total Antioxidant

The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for free radical scavenging activity, total antioxidant capacity and total phenolic content. All of the methanol extracts of the these plants and their kupchan fractions showed moderate to strong free radical scavenging activity, the total antioxidant capacity and total phenolic content, of which the methanol extract of the leaf of B. verrucosa revealed highest activity having IC50 value of 6.35 ?g/ml. All the extractives of three plants were also studied for their thrombolytic potential. Among the three plants the carbon tetrachloride soluble fraction and methanol extract of leaf and aqueous soluble fraction of bark of B. tomentosa, methanol extract of bark of B. stipularis and carbon tetrachloride soluble fraction of leaf of B. verrucosa exhibited highest thrombolytic activity with clot lysis value of 41.46%, 34.85%, 37.04%, 36.45% and 33.72%, respectively. Standard streptokinase was used as positive control which exhibited 61.50% lysis of clot while the negative control water revealed 2.56% lysis of clot.Keywords: Antioxidant; Free radical scavenging; Total antioxidant capacity; Phenolic content; Thrombolytic activity; Bridelia.© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v5i2.13568        J. Sci. Res. 5 (2), 343-351 (2013)

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

scholarly journals In VitroAntioxidant Properties of Three Varieties ofAllium sativumL. Extracts

2010 ◽  
Vol 7 (s1) ◽  
pp. S573-S579 ◽  
Author(s):  
R. T. Narendhirakannan ◽  
K. Rajeswari
Keyword(s):  
Free Radical ◽  
Allium Sativum ◽  
Preventive Intervention ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Garlic Bulb ◽  
Total Antioxidant ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Many herbs possess antioxidant ingredients that provide efficacy by additive or synergistic activities.Allium sativumL. is a strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. The antioxidant activities of different concentrations of ethanolic extracts of garlic bulb of three varieties were determined by the four assaysi.e. DPPH radical scavenging assay, reducing power ability, hydrogen peroxide scavenging assay and total antioxidant capacity. Due to its natural origin and potent free-radical scavenging ability,Allium sativumL. could be used as a potential preventive intervention for free radical-mediated diseases.

scholarly journals in vitro Screening of Sesamum indicum Seeds for Antioxidant, Phytochemical and Biological Properties

2020 ◽  
Vol 32 (9) ◽  
pp. 2262-2266
Author(s):  
SHERAZ KHAN ◽  
SANIYA HASHIM KHAN ◽  
INAM ULLAH KHAN ◽  
WALI INAM ◽  
MUHAMMAD ASIM ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Sesamum Indicum ◽  
Total Antioxidant

Herbal medicines has been the most cost-effective and valuable medical practice to cure diseases and emphasize modern health care treatment. The present research was conducted to assess the biological activities of 10 fractions obtained from methanolic extract which was derived from dried seeds of Sesamum indicum. Antioxidant activity was assessed using DPPH radical scavenging, total antioxidant capacity and total reducing power assays. Highest free radical scavenging activity (80.3 ± 1.36%), total antioxidant capacity (104.7 ± 4.04 μg AAE/mg) and ferric reducing power activity (238.76 ± 1.23 μg AAE/mg) was shown by fraction SE. Fraction SE showed the highest phenolic contents (63.72 ± 1.5 μg GAE/mg) while fraction SG sample showed highest flavonoid contents (54.62 ± 2.61 μg QE QE/mg). Antibacterial activity was performed against four selected bacterial strains including Staphylococcus aureus, Escherichia coli, Enterobacter aerogens and Bacillus subtilis. Highest inhibition was shown by fraction SD (11 ± 1.04 mm) against Staphylococcus aureus, fraction SH against Bacillus subtilis (11 ± 1.06 mm) and fraction SB against Escherichia coli. All fractions were found inactive against the selected fungal strains. While performing antileishmanial activity, fraction SC showed highest percent mortality (78%) of Leishmania tropica. In brine shrimp lethality bioassay, fraction SG showed significant LD50 value (23.48 μg/mL).

scholarly journals Biological Activities of Bellis perennis Volatiles and Extracts

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Neslihan Kavalcioğrlu ◽  
Leyla Açık ◽  
Fatih Demirci ◽  
Betül Demirci ◽  
Hülya Demir ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Plant Materials ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activity ◽  
Dpph Scavenging

Different parts of Bellis perennis were subjected to hydrodistillation and the products were subsequently analyzed by GC and GC/MS. Aqueous and methanol extracts were prepared from the aerial parts. Antioxidant [1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, reducing activity, total antioxidant] and antimicrobial activity of the plant materials were studied. The aqueous extracts showed higher DPPH scavenging activity (85.8% at 102.5 μg/mL) than the methanol extract. Reducing power was also observed for both tested extracts, where the formation of linoleic acid peroxides was more for the aqueous extract than the methanol extract. Antimicrobial activities of all plant materials were evaluated at varying concentrations against a panel of pathogens, but without pronounced inhibitory effect.

scholarly journals IN VITRO ANTI-DIABETIC AND ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF TINOSPORA SINENSIS

2017 ◽  
Vol 9 (2) ◽  
pp. 42 ◽  
Author(s):  
Anindita Banerjee ◽  
Bithin Maji ◽  
Sandip Mukherjee ◽  
Kausik Chaudhuri ◽  
Tapan Seal
Keyword(s):  
Plant Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Inhibitory Activities ◽  
Ethanol Extracts

Objective: The aim of the present study was to evaluate the alpha (α)-amylase and alpha (α)-glucosidase inhibitory activities and in vitro antioxidant activities of the 80 % aqueous ethanol extracts of Tinosporasinensis Lour (Merr.).Methods: The 80% aq. ethanol extract of the plant was prepared. The plant extract was examined for its antioxidant activity by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content. Different concentrations (2, 4, 8,10and 15 μg/ml) of the extract was subjected to α-amylase inhibitory and α-glucosidase inhibitory activities and IC50were calculated.Results: The study revealed that the different concentrations of the plant extract possessed a very good amount of total phenolics, flavonoid and flavonol and exhibited potent radical scavenging activity using DPPH and ABTS as a substrate. The ethanol extracts exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value1.093µg and 1.04µg dry extract respectively and well compared with standard acarbose drug.Conclusion: Thus, it could be concluded that due to the presence of antioxidant components the plant extracts could be used for the treatment of hyperglycemia, diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent components from the natural resources for the clinical development of antidiabetic therapeutics.

scholarly journals Antioxidant and Membrane Stabilizing Activities of Bark of Sonneratia apetala

2016 ◽  
Vol 19 (2) ◽  
pp. 147-151 ◽  
Author(s):  
Md Emdadul Hasan Mukul ◽  
Mohammad Salim Hossain ◽  
Sayed Koushik Ahamed ◽  
Pankaj Debnath ◽  
Mariyam Akter
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Sonneratia Apetala ◽  
Reducing Power Assay

This study was carried out to investigate the antioxidant activity in terms of free radical scavenging capacity and membrane stabilizing ability of methanol extract of bark of Sonneratia apetala. The total phenol content of the extract was measured by Folin-Ciocalteu reagent. The antioxidant potential was investigated using 2,2 diphenylpicrylhydrazyl (DPPH), reducing power assay and chelating power determination. The membrane stabilizing capacity was assessed by monitoring the hypotonic solution - and heat-induced haemolysis of human erythrocytes. The total phenolic content was found 50.75 mg/gm of gallic acid equivalent. The extract exhibited significant antioxidant activity in DPPH free radical scavenging assay with IC50 value of 81.42 µg/ml as compared to the standard, BHT (IC50=42.56 µg/ml), Fe2+ ion reducing power assay and chelating power ability. In case of assay for chelating ability, EDTA represented 98.76% chelation while the plant extract showed 82.83% at concentration of 100 ?g/ml. In addition, the methanol extract of bark of this plant was found to moderately inhibit the haemolysis of human erythrocyte. In conclusion, the methanol extract of S. apetala could be valuable candidate for future development for antioxidant activity.Bangladesh Pharmaceutical Journal 19(2): 147-151, 2016

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