Soluble Dietary Fiber from Aloe Vera and Lady's Finger; Effect on Glucose Absorption in Type-2 Diabetic Model Rats

Despite considerable progress in diabetes mellitus by conventional synthetic drugs, the search for natural anti-diabetic plant products is going on. The effects of soluble dietary fibre (SDF) of Aloe Vera and Lady’s Finger on glucose absorption were evaluated by the gut perfusion method in Type-2 diabetic model rats. SDFs were extracted from Aloe Vera and Lady’s Finger by enzymatic digestion method. Both the SDF extracts of Aloe Vera and Lady’s Finger and glucose (control) suspended in buffer solution at a dose of 1.25 g/kg were perfused at a rate of 0.5 mL/min for 30 min through the gut. The % of glucose absorption observed for up to 30 min. The % of glucose absorption for Aloe Vera and Lady’s Finger during the 30 min was 52.98±5.67 and 57.74±4.81, respectively, compared to control 67.74±8.62. The p-value (0.061) for Aloe Vera was quite closer to the level of significance. On the other hand, Lady’s Finger had a non-significant effect on glucose absorption (p=0.145) in Type-2 diabetic model rats' gut. The results suggested the therapeutic potential of the SDF of Aloe Vera and Lady’s finger, which suppressed postprandial hyperglycemia after glucose ingestion.

Download Full-text

Angiotensin II causes endothelial dysfunction via the GRK2/Akt/eNOS pathway in aortas from a murine type 2 diabetic model

Pharmacological Research ◽

10.1016/j.phrs.2011.05.001 ◽

2011 ◽

Vol 64 (5) ◽

pp. 535-546 ◽

Cited By ~ 34

Author(s):

Kumiko Taguchi ◽

Tsuneo Kobayashi ◽

Yasuhiro Takenouchi ◽

Takayuki Matsumoto ◽

Katsuo Kamata

Keyword(s):

Angiotensin Ii ◽

Endothelial Dysfunction ◽

Diabetic Model ◽

Type 2 Diabetic

Download Full-text

Antidiabetic effect of enterolactone in cultured muscle cells and in type 2 diabetic model db/db mice

Cytotechnology ◽

10.1007/s10616-016-9965-2 ◽

2016 ◽

Vol 69 (3) ◽

pp. 493-502 ◽

Cited By ~ 8

Author(s):

Fang Zhou ◽

Keisuke Furuhashi ◽

Myoung Jin Son ◽

Miku Toyozaki ◽

Fumiaki Yoshizawa ◽

...

Keyword(s):

Muscle Cells ◽

Antidiabetic Effect ◽

Diabetic Model ◽

Type 2 Diabetic

Download Full-text

The Effect of Resistance Training on G6Pase Gene Expression in Liver Hepatocytes, Glucose and Insulin Resistance Levels in Type 2 Diabetic Rats

Iranian journal of diabetes and obesity ◽

10.18502/ijdo.v12i1.3634 ◽

2020 ◽

Author(s):

Ahmad Shokrolahi Ardakani ◽

Hossein Abednatanzi ◽

Mandana Gholami ◽

Nader Shakeri

Keyword(s):

Resistance Training ◽

Training Group ◽

Liver Cells ◽

Diabetic Rats ◽

Exercise Session ◽

P Value ◽

Insulin Levels ◽

And Control ◽

Type 2 Diabetic

Objective: The aim of this study was to determine the effect of 12 weeks resistance training on G6Pase expression in liver cells, as well as glucose and insulin levels in type 2 diabetic rats. Materials and Methods: In this experimental study, 16 wistar rats were selected as the research sample. After injection of nicotinamide and streptozocin to induce diabetes, the rats were randomly divided into two groups of resistance training and control. The resistance group participated in a course of resistance training for up to 12 week in five sessions per week, with intensity of 75% and a time of 30 to 45 minutes. Finally, 48 hours after the last exercise session, G6Pase expression in liver cells, as well as glucose and insulin levels were measured in both groups. Results: Comparison of resistance and control training groups showed a decrease in glucose levels (P-value= 0.001) and increased insulin levels (P-value= 0.001). Exercise also reduced the expression of G6Pase in liver cells in the resistance training group (P-value= 0.001). Conclusion: Based on the results of the study, it is recommended that diabetics use resistance training under the supervision of a specialist to reduce the negative effects of diabetes.

Download Full-text

Comparative Study on Antidiabetic Effect of Ethanolic Extract of Jute Leaf on Neonatal Streptozotocin- Induced Type-2 Diabetic Model Rat

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i3130922 ◽

2020 ◽

pp. 60-71

Author(s):

Md. Mahabub Ali ◽

Md. Asrafuzzaman ◽

Md. Mahedi Hassan Tusher ◽

Md. Hafizur Rahman ◽

Md. Tanvir Rahman ◽

...

Keyword(s):

Body Weight ◽

Lipid Profile ◽

Ethanolic Extract ◽

Liver Glycogen ◽

Serum Glucose ◽

Water Control ◽

Citrate Buffer ◽

Diabetic Model ◽

Type 2 Diabetic

Aim: Functional food and their bioactive compounds have been considered as a new approach for the prevention and management of type 2 diabetes and its complications. According to this approach current study was carried out as an elucidation of antidiabetic properties of Corchorus capsularis and Corchorus olitorius varieties of jute leaf (ethanolic extract) on nSTZ-induced type-2 diabetic rats. Methodology: The type-2 diabetic model rat was developed by a single intraperitoneal injection of freshly prepared STZ (90 mg/kg/10 ml) in sterile citrate buffer (0.1 M, pH 4.5) to rat pups (48 hour old). After three months, OGTT was performed to select diabetic (FSG > 6.5mmol/L and after 90 min of glucose load > 14 mmol/L) experimental rats. The rats were randomly divided into four groups [DWC, GT, Ext-1 and Ext-2 represent, diabetic water control, glybenclamide treated (20 mg/5 ml/kg body weight), C. capsularis treated and C. olitorius treated group (1.25 g/10 ml/kg body weight) respectively]. One group was kept with normal rats [normal water control, NWC]. The treatment was given once daily or 28 consecutive days. Fasting serum glucose, liver glycogen and lipid profile were estimated by using standard methods. Results: The results showed that Ext-1 and Ext-2 treated groups gradually decreased serum glucose level (7.15 ±0.67 to 5.94 ± 1.19 and 7.20 ± 0.93 to 5.28 ±1.03 respectively) and reducing effect by Ext-2 was significant (p=0.001). Both extract showed lower liver glycogen level compared with GT group [5.0±2.5 Vs 17.7±6.5 (Ext-1 vs GT) and 7.5±6.4 Vs 17.7±6.5 (Ext-2 vs GT)] and even Ext-1 manifested significant effect (p=0.05). Additionally, lipid profile estimation revealed no significant improvement by the consumption of both the extracts. Conclusion: On the basis of current investigations, it may be concluded that both variety of jute’s leaf demonstrated hypoglycemic properties in Type 2 diabetic model rats; further in-depth studies are recommended to explore the exact mechanism(s) of hypoglycemic effect.

Download Full-text

EFFECT OF ALOE BARBADENSIS MILLER WHOLE LEAF EXTRACT ON HYPERGLYCEMIA AND INSULIN RESISTANCE IN LOW DOSE STREPTOZOTOCIN INDUCED TYPE 2 DIABETIC RATS

Journal of Saidu Medical College, Swat ◽

10.52206/jsmc.2013.3.2.350-355 ◽

1969 ◽

Vol 3 (2) ◽

pp. 350-355

Author(s):

MEENA GUL ◽

MUHAMMAD MAZHAR HUSSAIN ◽

AYESHA BABER ◽

AMJAD ZAMAN ◽

MUSRAT ZAHRA

Keyword(s):

Insulin Resistance ◽

Body Weight ◽

Plasma Glucose ◽

Leaf Extract ◽

Low Dose ◽

Aloe Vera ◽

Control Group ◽

Sprague Dawley ◽

Type 2 Diabetic

BACKGROUND: Managing diabetes is difficult due to the number of side effects associated with drugsused for its treatment. There it is a need of an hour to look for indigenous plants which are safe and costeffective. Present study was planned to determine the effect of Aloe vera whole leaf extract and/orRosiglitazone on plasma glucose, insulin and insulin resistance in type 2 diabetic Sprague-Dawley rats.DESIGN: Randomized control trailPLACE AND DURATION OF STUDY: This study was conducted from April 2009 to Oct 2010 at theDepartment of Physiology Army Medical College, Rawalpindi in collaboration with National Institute ofHealth (NIH) Islamabad.MATERIAL AND METHOD: Type 2 DM was induced in 60 healthy Sprague-Dawley rats by feedinghigh fat diet for 2 weeks and injecting a low dose (35mg/kg) of streptozotocin intra peritoneally. Type 2diabetic rats were randomly divided into four groups, each group having 15 rats and were labeled as diabeticgroup, Aloe vera group, rosiglitazone group and combined group. The diabetic group was injected normalsaline, Aloe vera group was treated with Aloe vera whole leaf extract in dose of 300mg/kg body weight,rosiglitazone group was given 5mg/kg body weight of rosiglitazone I/P and combined group diabetic ratswere treated with 150mg/kg body weight of Aloevera extract and 2.5mg/kg body weight of rosiglitazone(halfof their effective dose) for 21 days.RESULTS: A significant reduction (p<0.001) in plasma glucose (73%), insulin (32%) and TG/HDL ratio(81%) was analyzed in combined groupascompared to diabetic control group. \CONCLUSION: The maximum impact in lowering plasma glucose, insulin and TG/HDL ratio wasrecorded in combined group, followed by rosiglitazone group and then Aloevera group.KEYWORDS:T2DM. Aloe vera, insulin resistance

Download Full-text