scholarly journals Investigation of microbiological spoilage and demonstration of the anti-bacterial activity of the major imported fruits within Dhaka Metropolis

2015 ◽  
Vol 4 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Farahnaaz Feroz ◽  
Jessica Das Senjuti ◽  
Jannatun Tahera ◽  
Kamal Kanta Das ◽  
Rashed Noor
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Activity ◽  
Huge Number ◽  
Pseudomonas Spp ◽  
Dragon Fruit ◽  
Fruit Samples ◽  
Fungal Load ◽  
Viable Bacteria ◽  
Alcoholic Extraction

With a previous observation of the in vitro anti-bacterial traits posed by both local and imported fruit alcoholic extracts, present study further endeavored principally to chalk out such properties of the imported fruit blends without alcoholic extraction. Experiments regarding microbiological load demonstrated the prevalence of huge number of total viable bacteria (~107 cfu/g) as was also observed in the earlier research while the fungal load was nominal. Among the pathogenic bacteria, Pseudomonas spp. was found to be more prevalent, closely followed by staphylococcal proliferation. The study of anti-bacterial activity revealed that guava, apple and malta samples possessed strong anti-bacterial traits while the dragon fruit samples exhibited least activity and the orange samples were found with moderate anti-bacterial activity. On the basis of our earlier results, the findings of the current study thus put forward a comparison among the alcoholic extracts and the crude fruit blends. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22752 Stamford Journal of Microbiology, Vol.4(1) 2014: 1-4

scholarly journals In vitro anti-bacterial activity of Prosopis juliflora leafs extract against pathogenic bacteria

2017 ◽  
Vol 5 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Ali M. Badri ◽  
◽  
Mohamed I. Garbi ◽  
Ahmed S. Kabbashi ◽  
Mahmoud S. Saleh ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Prosopis Juliflora ◽  
Bacterial Activity

scholarly journals Microbiological safety of commonly available eye ointments and their in-vitro anti-bacterial potency

2019 ◽  
Vol 35 (2) ◽  
pp. 122-126
Author(s):  
Mrityunjoy Acharjee ◽  
Rakhi Baishnaib ◽  
Rashed Noor
Keyword(s):  
Microbiological Quality ◽  
Positive Control ◽  
Fungal Contamination ◽  
Zone Diameter ◽  
Pseudomonas Spp ◽  
Cultural Methods ◽  
Bacillus Spp ◽  
Viable Bacteria ◽  
Staphylococcus Spp

Current study attempted to check out the microbiological quality of some common sterile ointment commonly implemented for the treatment of eye infections in Bangladesh. Seventeen (17) different eye ointment (T-Mycin, Aprocin, Bactin, Optimox and Cloram, Hypomer gel, Sonexa, Polytracin, Cero, Aristobet, Lotepred, Herpigel, Gentob, Xoviral, Zirgan, Xovir, Tomycin, Tobirax, AFm-plus and Parafresh) were microbiologically examined through common and traditional cultural methods. Total viable bacterial and fungal contamination was found up to 106 and 105 cfu/ml respectively. Among the 17 samples T-Mycin, Aprocin, Sonexa, Polytracinand Tomycinwere free from fungal contamination. All the samples significantly exceeded United States Pharmacopeia (USP) or British Pharmacopeia (BP) limit (<102 cfu/ml) in case of Total viable bacteria and fungus contamination. While the coliforms (Escherichia coli and Klebsheilla spp.) were absent in all samples, the prevalence of Staphylococcus spp. was 100% in all samples up to 103 while the Bacillus spp. was found up to 102 cfu/ml. Pseudomonas spp. was cultivated in TMycin, Aprocin Hypomer gel, Sonexa, Polytracin, Cero, Aristobet and Lotepred up to 103cfu/ml. All the 5 drugs showed their antibacterial potency with satisfactory range of zone diameter againstE. coli, Pseudomonas spp., Staphylococcus spp., Klebsheilla spp. and Bacillus spp. In case of all 17 ointments the average zone diameter range was noticed within 13mm-22mm. The highest zone diameter (22mm) was recorded against E. coli produced by Aprocin and minimum zone diameter (13mm) was recorded against Bacillus spp. produced by Bactin, Optimox and Cloram.Gentamicin and Streptomycin were used as positive control against the tested bacteria. Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 122-126

scholarly journals Chemical composition and antimicrobial activity of fruit essential oils of Myrica gale, a neglected non-wood forest product

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Kristina Ložienė ◽  
Juozas Labokas ◽  
Vaida Vaičiulytė ◽  
Jurgita Švedienė ◽  
Vita Raudonienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Chiral Phase ◽  
Myrica Gale ◽  
Broth Microdilution Method ◽  
Fruit Samples

The study aimed to establish the chemical composition of fruit essential oils of M. gale and test their activities against the selected pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii), yeasts (Candida albicans, C. parapsilosis), fungi (Aspergillus fumigatus, A. flavus) and dermatophytes (Trichophyton rubrum, T. mentagrophytes). Fruit samples from natural (Western Lithuania) and anthropogenic (Eastern Lithuania) M. gale populations were studied separately. Essential oils were isolated from dried fruits by hydrodistillation and analysed by GC/FID and GC/MS methods; enantiomeric composition of α-pinene was established by chiral-phase capillary GC. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) of essential oils were determined using the broth microdilution method. Plants from the natural population with a humid marine climate accumulated significantly higher amounts of fruit essential oils (3.34±0.05%) than those from the anthropogenic population with a more continental climate (2.71±0.22%). In total, 39 volatiles including α-pinene (23.52–27.17%), 1,8-cineole (17.19–18.84%) and α-phellandrene (9.47–10.03%) as main compounds were identified. Chiral analysis demonstrated that (1S)-(–)-α-pinene prevailed over (1R)-(+)-α-pinene and amounted to 94.09–95.28% of all fraction of this monoterpene. The antimicrobial study in vitro indicated that C. parapsilosis, dermatophytes and Aspergillus fungi were more susceptible to fruit essential oils of M. gale, whereas E. coli and C. albicans were weakly inhibited even at the highest essential oil concentration. The strongest growth-inhibitory and bactericidal effect of sweet gale essential oil was established on S. aureus. This could be attributed to the major essential oil compounds with known antimicrobial activity, such as α-pinene, 1,8-cineole and a-phellandrene. Keywords: Myrica gale; essential oil; chemical compounds; terpenes; enantiomers; antimicrobial.

scholarly journals The Inhibitory Effect of some Plant Extracts on some Pathogenic Bacteria

2019 ◽  
Vol 24 (1) ◽  
pp. 62
Author(s):  
Yasmeen I. Al-Hadidy ◽  
Sabraa S. Yaseen ◽  
Ghazwan M. Saleh
Keyword(s):  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Clinical Importance ◽  
Eucalyptus Camaldulensis ◽  
Inhibitory Effect ◽  
Assay Method ◽  
E Coli ◽  
Eucalyptus Leaves ◽  
Alcoholic Extraction

The purpose of the present study is to assay the inhibitory effect of five concentrations 10, 20, 30, 40 and 50 % of the aqueous and alcoholic extracts for leaves of the Sidr (Zizyphusspin-csit) and Eucalyptus leaves (Eucalyptus camaldulensis) in vitro, against the three types of the experimental bacteria, which Escherichia coli, Staphylococcus aureus and Salmonella typhimurium for their clinical importance as common, pathogenic in urinary tract infection by using the well diffusion assay method .The results showed that antimicrobial activity depends on kind of extract and tested microorganism. and the most bioactivity was done by methanol extract than the aqueous extract, the methanolic extract of Sidr leaves presented  the inhibitory effectiveness of all the species used  in this study ,the highest inhibition was 36 mm at 50%concentration against E. coli and methanolic extract of eucalyptus leaves showed an inhibitory effect on the growth of all bacteria except E. coli, showed resistance to the extract, As for the aquatic extracts, all the bacteria species illustrated resistance to the aquatic extracts of the leaves of the Sidr except Staph .aureus Inhibition rates were recorded at 17 and 15 mm at the concentration (30 and 20%), respectively, and the aquatic extracts of Eucalyptus leaves had no inhibitory effect on the growth of all the species used in this study, except S. typhimurium bacteria and all concentrations used. The  result of the qualitative chemical detection of the active compounds found in the extract indicated that it s contains, mainly like flavonids, alkaloids, Tannins and phenols, Saponins, Glycosides. in aquatic and alcoholic extraction.     http://dx.doi.org/10.25130/tjps.24.2019.010

scholarly journals Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers

2015 ◽  
Vol 4 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Marufa Sharmin ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Salmonella Spp ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23

scholarly journals Presence of microorganisms in commonly used baby cosmetics, available in Dhaka City

2020 ◽  
Vol 9 (1) ◽  
pp. 9-11
Author(s):  
Farahnaaz Feroz ◽  
Kamal Kanta Das
Keyword(s):  
Production Process ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Storage Conditions ◽  
Dhaka City ◽  
Acceptable Limit ◽  
Fungal Load ◽  
Viable Bacteria ◽  
And Storage

Cosmetics especially baby products should be safe as children are immunocompromised which means their immune system is not developed enough to combat infections caused by the presence of any pathogenic bacteria. Different factors like chemical composition of cosmetics, handling and storage conditions can influence the chance of contamination of cosmetic products. This study aimed to evaluate the microbiological quality of different commercially available baby cosmetics such as body lotion, body wash or soap, baby shampoo, baby oil of different popular brands sold in Dhaka city. In the current investigation four different brands of cosmetics were subjected to bacteriological and mycological screening. Total viable bacteria and fungal load in all the samples of Brand 1 was in average of 105 cfu/g which exceeded the acceptable limit. The samples were found to contain pathogenic bacteria as well, especially Klebsiella spp. The findings indicate that the quality Brand 1 products were not satifactory and might cause harm to children and other consumers. Microbial load of other brands fell within the acceptable limit which indicate their good quality. The incidence of microorganisms in Brand 1 might be due to contamination during the production process or storage under unhygienic condition. Therefore in order to avoid infections in children due to microbial contamination of cosmetics items it is essential to take precautions during production process and storage in appropriate conditions and avoiding use of cosmetics products on damaged skin. Stamford Journal of Microbiology, Vol.9(1) 2019: 9-11

scholarly journals Isolation and quantification of bacterial isolates from citrus fruits and determination of their anti-bacterial activity against selected pathogens

2015 ◽  
Vol 3 (1) ◽  
pp. 30-33
Author(s):  
Rowshan Akter ◽  
Nusrat Islam Tanu ◽  
Md Aftab Uddin
Keyword(s):  
Fungal Growth ◽  
Bacterial Activity ◽  
Citrus Limon ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Fruit Samples ◽  
Staphylococcus Spp ◽  
Vibrio Spp

The present study was undertaken with the aim of investigating the isolation and quantification of microorganisms from the citrus fruit samples collected from different areas of Dhaka city. Out of ten samples studied, the range of total viable bacterial proliferation was approximately 102 to 107 cfu/g. Among the specific bacterial pathogens, prevalence of Klebsiella spp. was found in five samples, Listeria spp., Staphylococcus spp. and Pseudomonas spp. in four samples and Vibrio spp. in three samples only. The presumptive identification of these isolates was done by the conventional cultural, microscopic and biochemical tests. Fungal growth was also observed in four samples within the range of 1.2×103 to 3.6×103 cfu/g. Interestingly, Prunus mume/domestica (plum) showed the anti-bacterial activity against all the laboratory bacterial isolates tested. Among other samples, Tanarindus bacilus (tamarind) was found to exhibit activity against Escherichia coli, Pseudomonas spp., Salmonella spp., Vibrio spp., and Listeria spp. On the other hand, Monifera indica (mango) showed anti-bacterial efficacy against E. coli, Staphylococcus spp. and Listeria spp. and Citrus limon (lemon) only against Pseudomonas spp. and Listeria spp. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22750 Stamford Journal of Microbiology, Vol.3(1) 2013: 30-33

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

2018 ◽  
Vol 17 (6) ◽  
pp. 167-174 ◽  
Author(s):  
Małgorzata Schollenberger ◽  
Tomasz M. Staniek ◽  
Elżbieta Paduch-Cichal ◽  
Beata Dasiewicz ◽  
Agnieszka Gadomska-Gajadhur ◽  
...  
Keyword(s):  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Mentha Piperita ◽  
Mentha Spicata ◽  
Plant Essential Oils ◽  
Plant Pathogenic Bacteria ◽  
Interspecies Hybrids

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

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