scholarly journals Occurrence of pathogenic microorganisms in dessert items collected from Dhaka city

2020 ◽  
Vol 9 (1) ◽  
pp. 19-22
Author(s):  
Shohana Akter ◽  
Anasua Sarkar ◽  
Kamal Kanta Das
Keyword(s):  
Heterotrophic Bacteria ◽  
Good Health ◽  
Poor Quality ◽  
Dhaka City ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Due to delicious taste and readily availability, desserts are one of the most popular foods in Dhaka city. High amount of carbohydrate and protein in dessert items make them more susceptible to proliferation of microbial growth. Present study depicted a complete microbiological profile of some popular desserts such as, sweet, pastry, ice cream, pudding, falooda, yogurt and custard available in different food shop at Dhaka city, Bangladesh. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 106 cfu/g. In case of specific microflora, the growth of Staphylococcus spp., Klebsiella spp. and Pseudomonas spp. were observed in most of the samples indicates the poor quality of these products. Bioburdens of E. coli in sweet, pudding and yogurt were found in the range of 1.2×102 to 2.7×103 cfu/g. Salmonella spp., Shigella spp. and Vibrio spp. could not be isolated from any of the samples. Current study indicates that hygienic conditions should be maintained during preparation, packaging and retailing of dessert items in order to reduce the load of contamination in ready to eat foods which will ensure good health of consumers. Stamford Journal of Microbiology, Vol.9(1) 2019: 19-22

scholarly journals Isolation of Multi-Drug Resistant Potential Pathogenic Bacteria From Blow Fly Collected From Different Areas of Dhaka City

2021 ◽  
Vol 49 (2) ◽  
pp. 205-214
Author(s):  
Tangin Akter ◽  
Samira Jahan ◽  
Sangita Ahmed ◽  
Shanjida Sultana ◽  
Shefali Begum
Keyword(s):  
Pathogenic Bacteria ◽  
Dhaka City ◽  
Blow Fly ◽  
Salmonella Spp ◽  
Blow Flies ◽  
Fish Market ◽  
Shigella Spp ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The research work was conducted for isolation and identification of potential pathogenic bacteria from blow fly (Calliphora spp.) and to investigate their antibiotic susceptibility. For this purpose, 20 blow flies were collected from 4 selected areas (Curzon Hall, Dhaka Medical College Hospital, Fruit Store and Fish Market) of Dhaka city. A total of 40 samples (20 from external surface and 20 from alimentary gut homogenates) were prepared from the blow flies. Among the total 20 flies, 3(15%) were collected from Curzon Hall and 5(12.5%) isolates were obtained from them; 6(30%) flies were collected from DMCH which had 11(27.5%) isolates; 5(25%) flies were collected from fruit stores and 10(25%) isolates were collected from there; 6(30%) flies were collected from fish market and 14(35%) isolates were collected from there. The isolated bacteria were presumptively identified as Escherichia coli, 12 (30%); Salmonella spp., 8 (20%); Shigella spp., 4(10%); Enterobacter spp., 3 (7.5%); Klebsiella spp., 2 (5%); Bacillus spp., 7 (17.5%) and Staphylococcus spp., 4 (10%) in number which were based on morphology as observed under microscope as well as cultural and biochemical properties. All of these isolates were resistant to various antibiotics. Bacterial susceptibility showed that E. coli isolates were highly resistant (66.6%) to Penicillin, Salmonella spp. mostly resistant (62.5%) to Penicillin and Tetracycline. Klebsiella spp. Isolates were 100% resistant to Penicillin and 100% sensitive to Ciprofloxacin, Imipenem and Tetracycline. In case of Shigella spp. and Enterobacter spp., 75% and 100% isolates were resistant to Penicillin respectively. Among the Staphylococcus spp. isolates, 75% were resistant to Ampicillin and Penicillin. In case of Bacillus spp. isolates, 57.2% were resistant to Penicillin and 100% sensitive to Imipenem. Antibiotic resistance of these species affect our human health also. This study demonstrated the potential of blow flies as a vector of various pathogenic microorganisms and a mode of transmission of antibiotic resistance. Bangladesh J. Zool. 49 (2): 205-214, 2021

scholarly journals Prevalence and Antibiogram Profiling of Rotten Fruits from Different Areas of Dhaka City, Bangladesh

2020 ◽  
pp. 1-9
Author(s):  
Mahmuda Akhter Akhi ◽  
Avijit Banik ◽  
Oshin Ghurnee ◽  
Nantu Chandra Das ◽  
Showmitro Nondi ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Street Vendors ◽  
E Coli ◽  
Daily Diet ◽  
Acinetobacter Spp ◽  
Klebsiella Spp

Fruits are highly nutritious, sources of vitamins, minerals, fibers etc. and these are part of our daily diet. However, during cultivation, harvesting, transportation, handling fruits get contaminated with pathogenic microorganisms which leads to severe problems to community. Owning to this point, in current research, bacteriological analysis was performed on Sofeda, Pineapple, Grape, Banana, Apple, Orange, Guava, papaya, Jujube and Starfruit. Total 50 samples were randomly collected from market and street vendors of Dhaka city. Higher numbers of rotten fruits were present in wholesale markets. 35 strains were isolated which included Salmonella spp., Acinetobacter spp., Klebsiella spp., Vibrio cholerae, Vibrio parahaemolyticus, Staphylocoous aureus and E. coli. They were identified using biochemical test and antibiogram for selective isolates. In case of drug resistance of isolates, majority exhibited resistance against Erythromycin, Vancomycin and Amoxycillin and showing sensitivity against Ciprofloxacin and Ceftriaxone. It was observed in the current study that 100% isolates were resistant against Erythromycin, followed by Amoxycillin 90.63% and vancomycin 86.25%, where only 35.27% isolates were resistant against Ciprofloxacin. In case of sensitivity 64.73% isolates were sensitive against Ciprofloxacin followed by Ceftriaxone 66.25%. 

scholarly journals Bacterial Assessment of Unpasteurized Fruit Juices Sold in Port Harcourt, Nigeria

2020 ◽  
pp. 1-7
Author(s):  
Constancy Prisca Aleru ◽  
Vivian Nkemkanma Agi ◽  
Kingsley Njoku
Keyword(s):  
Fruit Juices ◽  
Plate Count ◽  
Heterotrophic Plate Count ◽  
E Coli ◽  
Port Harcourt ◽  
Bacillus Spp ◽  
Tiger Nuts ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

This study assessed the bacterial quality of fresh fruit juices sold in some restaurants in Port Harcourt, Nigeria. A total of twenty (20) fruit juice samples were collected from four (4) different types of fruit juices sold at four (4) different restaurants. The fruits that were used for this study are: Orange, Watermelon, Pineapple and Tiger nuts. Samples of the fruit juices were collected for bacteriological assessment using heterotrophic plate count and most probable number techniques, while CHRO Magar for E. coli and other coliforms was used for the detection of E. coli. The results showed that E. coli, Bacillus spp, Klebsiella spp, Streptococcus spp and Staphylococcus spp were present in the fruit juices. The results of the heterotrophic plate count showed that the bacterial count ranged from 2.7 x 102 (Orange) to 7.1 x 103 (Tiger nuts), while the results of the total coliform count ranged from 11 MPN/100 ml to 28 MPN/100 ml. However, the study revealed that the total E. coli count ranged from 0 to 7. The number of E. coli in each of the fruit juices and restaurants ranged from 6 to 13, Bacillus spp 12 to 19, Klebsiella spp 4 to 10, Streptococcus spp 6 to 11 and Staphylococcus spp 4 to 13. Bacillus spp had the highest percentage (31.4%), followed by Staphylococcus spp (20.1%). The highest percentage of bacteria was recovered from Tiger nuts (29.4%), followed by Watermelon (28.4%). Regular monitoring of the quality of fresh fruit juices sold in restaurants in Port Harcourt and other parts of Nigeria should therefore be enforced.

scholarly journals Evaluation Microbiologique Et Origines De La Contamination Des Produits De 4ème Gamme Vendus Sur Les Marchés D’abidjan, Cote D'ivoire

2016 ◽  
Vol 12 (36) ◽  
pp. 273
Author(s):  
Louise Atchibri Anin ◽  
Patrice Désiré Yapi Assoi Yapi ◽  
Yapo Thierry Monnet ◽  
Marie-Ange Yiwo Yapi ◽  
Chantal Lêniféré Soro ◽  
...  
Keyword(s):  
Food Contamination ◽  
Poor Quality ◽  
Raw Material ◽  
Ananas Comosus ◽  
Salmonella Spp ◽  
E Coli ◽  
Quality Of Water ◽  
Tomato Puree ◽  
The City

The sources and degree of microbial contamination of 4 th range products or ready-to-eat products made from traditional processes were evaluated in three markets in the city of Abidjan. This study initially consisted of searching for microorganisms such as Staphylococcus aureus, faecal coliforms, E. coli and Salmonella spp. Then, Ishikawa's method or diagram was used to determine potential sources of food contamination consisting of three (3) fruit and two (2) fruit vegetables and one (1) leafy vegetable, respectively, pineapple (Ananas comosus) papaya (Carica papaya), watermelon (Citrulus lanatus), onion (Allium cepa), tomato (Lycopersicum esculentum Mill.) and amaranthus (Amaranthus hybridus). Fecal coliform loads were very high at the fruit level (1.8 × 103 - 9.1 × 102 CFU / g), onion (1.3 × 104 CFU / g), tomato puree (1, 82 × 103 CFU / g) and an E. coli load at the tomato puree which is 9.1 × 102 CFU / g. The analysis using the Ishikawa diagram shows that contamination sources were the poor quality of water, raw material, lack of sanitation, illiteracy, unsafe traditional practices and sellers hygiene.

scholarly journals Identification and antibiogram study of bacteria isolated from different street food

2018 ◽  
Vol 4 (3) ◽  
pp. 279-287
Author(s):  
Mohamed Ahmed Khalif ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Md Aoulad Hosen
Keyword(s):  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Wide Spectrum ◽  
Salmonella Spp ◽  
Street Vendor ◽  
Street Food ◽  
Food Borne ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Food borne diseases are an increasingly recognized problem involving a wide spectrum of illnesses caused by bacterial contamination of food.Microorganism poses potential human health problems and is mainly transmitted through consumption of contaminated foods .Bangladesh is one of the densely populated country where majority of the people consume cheap foods prepared in unlicensed food selling points. The objective of the present study was to investigate the microbiological quality of different street food (Amra, Fusca, Chanachur and Guava) sold by various street vendor at Dinajpur, Bangladesh. A total of 20 samples were collected randomly from street vendors and tested for the presence of bacteria following standard microbiological method used for isolation, enumaration and identification of bacreria. Among the samples all had bacterial contamination. The total viable count (TVC) in diferrent street food samples was ranged from 8.0 × 105 CFU/g to 6.7x107 CFU/g. Among samples Escherichia coli 7(35%), Staphylococcus spp. 6(30%), Klebsiella spp. 5(25%), Salmonella spp. 1(5%) and Shigella spp. 1 (5%) were isolated. Escherichia coli were found highest 3(50%) Amra samples and 2(40%) Guava samples, then another organisms Staphylococcus spp. was found highest 2(50%) Chanachur and 2(40%) Fusca samples and then Klebsiella spp. was found highest 2(40%) Fusca samples. Antibiotic sensitivity test showed that Shigella spp., Staphylococcus spp., Klebsiella spp., Salmonella spp. and Escherichia coli were sensitive to Ciprofloxacin and Salmonella spp. were resistance to Gentamycin. All isolates found resistant to Cefixime, Cefalexin, Erythromycin, Fusidic acid, Cefuroxime and Aztreonam. All of the sample harbor multidrug resistant food borne bacteria which might cause public health hazards if these antibiotic resistance transfer to human.Asian J. Med. Biol. Res. September 2018, 4(3): 279-287

scholarly journals Prevalence of Pathogenic Bacteria in Shrimp Samples Collected from Hatchery, Local Markets and the Shrimp Processing Plant

2016 ◽  
Vol 29 (1) ◽  
pp. 7-10 ◽  
Author(s):  
M Majibur Rahman ◽  
Farjana Rahman ◽  
Farzana Afroze ◽  
Farzana Yesmin ◽  
Kazi Kaniz Fatema ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Processing Plant ◽  
Salmonella Spp ◽  
Economic Interests ◽  
Local Markets ◽  
E Coli ◽  
Export Quality ◽  
Klebsiella Spp ◽  
Vibrio Spp

Export quality frozen shrimps comprise as one of the major economic interests in Bangladesh. During cultivation or processing and packaging, microbial condition is an important issue to maintain the quality of shrimps. Eight shrimp samples, of which, one from hatchery, 3 from local markets and 4 processed export quality samples were studied for microbiological risk assessment (MRA). One hatchery, three market and two export quality shrimp samples were found to contain bacterial pathogens probationary identified as Escherichia coli, Klebsiella spp., Vibrio spp., Aeromonas spp., Pseudomonas spp., Listeria spp., Shigella-like organisms, Staphylococcus aureus, and Salmonella spp. and two other processed export quality shrimp samples were completely free of pathogens of any type. Surprisingly, the later two processed export quality shrimp samples showed antibacterial activity against E. coli, Klebsiella sp. and Vibrio sp.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 7-10

scholarly journals Evaluation of microbial contamination level and the drug susceptible pattern of the isolates cultivated from famous dessert food

Food Research ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 426-430
Author(s):  
T. Akter ◽  
T. Ishma ◽  
S.R. Razzeb ◽  
S.H.M. Uddin ◽  
M. Islam ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
Heterotrophic Bacteria ◽  
Ice Cream ◽  
Drug Susceptibility ◽  
Diffusion Method ◽  
Contamination Level ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Present study endeavored to evaluate the microbial contamination level along with their drug resistant pattern in some popular desert food items collected from different food shops in Dhaka city, Bangladesh. The microbial evaluation was conducted through conventional cultural methods and drug susceptibility test was executed through disc diffusion method. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 105 CFU/g. In case of specific microflora, the growth of Staphylococcus spp. was very high in sweet, faluda, milk cake and ice cream as estimated up to 105 CFU/g, halua and sweet yogurt showed 104 CFU/g while rest of the samples revealed 103 CFU/g. E. coli was found only in faluda and ice cream up to 103 CFU/g whereas Klebsiella spp. was estimated in all the samples within the range of 102 CFU/g to 105 CFU/g. Salmonella spp., Pseudomonas spp. and Bacillus spp. were totally absent in all the samples. Most of the isolates were found to be resistant against most of the antibiotics. Meanwhile, streptomycin (10 µg), gentamicin (10 µg), azithromycin (15 µg), and nalidixic acid (5 µg) were effective drug against both E. coli and Staphylococcus spp.

scholarly journals Bacteriological Quality Assessment of Raw Beef Sold in Sylhet Sadar

2016 ◽  
Vol 13 (2) ◽  
pp. 9-16 ◽  
Author(s):  
Farhana Jahan ◽  
A T M Mahbub-E-Elahi ◽  
A B Siddique
Keyword(s):  
Viable Count ◽  
Total Viable Count ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Fresh Meat ◽  
Gram Negative ◽  
Bacteriological Quality ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

This study was conducted to assess the bacteriological quality of fresh raw beef sold in different markets of Sylhet Sadar. A total of seventy five (75) fresh raw beef samples were randomly collected from seven  major markets (Shibgonj, Mirabazar, Kazitula, Ambarkhana, Madina market, Bandar bazar and Sheikh ghat) of Sylhet Sadar. Total Viable Count of the samples ranged between 2.5 × 105 to 2.25 × 108cfu/g. Acceptability for consumption of these samples as satisfactory, acceptable and rejected were 40, 32 and 28%, respectively. A total of 115 bacterial isolates of 5 genera were identified including gram negative Escherichia coli [15(10%)], Salmonella spp. [20(13.33%)], Klebsiella spp. [30(20%)], Enterobacter spp. [10(6.67%)] and gram positive Staphylococcus spp. [40(26.67%)]. The presence of these organisms in fresh meat from conventional beef is alarming.DOI: http://dx.doi.org/10.3329/agric.v13i2.26654The Agriculturists 2015; 13(2) 09-16

scholarly journals Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers

2015 ◽  
Vol 4 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Marufa Sharmin ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Salmonella Spp ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23

scholarly journals Perfil de resistência e ou sensibilidade antibacteriana de enterobactérias coletadas de ovos de p. expansa

2020 ◽  
Vol 11 (5) ◽  
pp. 149-158
Author(s):  
Rosildo Mendes Evangelista Sobrinho ◽  
Adriana Malvásio ◽  
Aparecido Osdimir Bertolin
Keyword(s):  
Salmonella Spp ◽  
E Coli ◽  
De Se ◽  
Shigella Spp ◽  
Klebsiella Spp

A ordem Testudines conhecidos atualmente como quelônios, compreendem as tartarugas, jabutis e cágados). Esses animais são ovíparos e depositam seus ovos em diferentes ambientes. Diante do exposto objetivou-se identificar e traçar o perfil de resistência e ou sensibilidade antimicrobiana de enterobactérias coletadas de ovos P. expansa. Os microrganismos foram coletados de 10 ninhos na praia alta município de Lagoa da Confusão-TO. Foram identificadas 1.200 colônias de enterobactérias, distribuídas entre os gêneros Enterobacter ssp. Citrobacter spp. Shigella spp. Hafnia ssp. Klebsiella spp. Proteus spp. Salmonella spp.  e a espécie E. coli. sendo Enterobacter ssp. (33,33%), Citrobacter spp. (20,83%), Shigella spp. (12,5%), E. coli (20,83%) Hafnia ssp. (8,33%), Klebsiella spp. (2,08%), Proteus spp. (1,67%), Salmonella spp. (0,42%). Quanto ao perfil de resistência e ou sensibilidade obtidos foi detectado multirresistência aos antimicrobianos em todos os microrganismos, alguns com resistência a mais de 03 dos antibióticos testados. Os resultados obtidos neste estudo ressaltam a importância de se conhecer o perfil de enterobactérias isoladas de ovos de P. expansa, tendo em vista que tais bactérias são encontradas como integrantes da microbiota residente e transitória de P. expansa e da areia das praias. E. coli, Shigella spp., Hafnia spp., e Citrobacter spp. foram os microrganismos que apresentaram resistência ao maior número de antimicrobianos utilizados. Enterobacter spp, E. coli e Citrobacter spp. foram as enterobactérias encontradas em maior número de ovos, podendo estar relacionadas com a microbiota da areia da praia, contaminando os ovos por via horizontal.

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